Performance of a simple Closed Aquatic Ecosystem (CAES) in space.

A simple Closed Aquatic Ecosystem (CAES) consisting of single-celled green algae (Chlorella pyrenoidosa, producer), a spiral snail (Bulinus australianus, consumer) and a data acquisition and control unit was flown on the Chinese Spacecraft SHENZHOU-II in January 2001 for 7 days. In order to study the effect of microgravity on the operation of CAES, a 1 g centrifuge reference group in space, a ground 1 g reference group and a ground 1 g centrifuge reference group (1.4 g group) were run concurrently. Real-time data about algae biomass (calculated from transmission light intensity), temperature, light and centrifugation of the CAES were logged at minute intervals. It was found that algae biomass of both the microgravity group and the ground 1 g-centrifuge reference group (1.4 g) fluctuated during the experiment, but the algae biomass of the 1 g centrifuge reference group in space and the ground 1 g reference group increased during the experiment. The results may be attributable to influences of microgravity and 1.4 g gravity on the algae and snails metabolisms. Microgravity is the main factor to affect the operation of CAES in space and the contribution of microgravity to the effect was also estimated. These data may be valuable for the establishment of a complex CELSS in the future.     

Possible nutrient limiting factor in long term operation of closed aquatic ecosystem

To investigate nutrient limitation effect on the community metabolism of closed aquatic ecosystem and possible nutrient limiting factors in the experimental food chains, depletion of inorganic chemicals including carbon, nitrogen and phosphorous was tested. A closed aquatic ecosystem lab module consisting of Chlorella pyrenoidosa and Chlamydomonas reinhardtii, Daphnia magna and associated unidentified microbes was established. Closed ecological systems receive no carbon dioxide; therefore, we presumed carbon as a first limiting factor. The results showed that the algae population in the nutrient saturated group was statistically higher than that in the nutrient limited groups, and that the chlorophyll a content of algae in the phosphorus limited group was the highest among the limited groups. However, the nitrogen limited group supported the most Daphnia, followed by the carbon limited group, the nutrient saturated group and the phosphorus limited group. Redundancy analysis showed that the total phosphorus contents were correlated significantly with the population of algae, and that the amount of soluble carbohydrate as feedback of nutrient depletion was correlated with the number of Daphnia. Thus, these findings suggest that phosphorus is the limiting factor in the operation of closed aquatic ecosystem. The results presented herein have important indications for the future construction of long term closed ecological system.     

Optimal station-keeping near Earth–Moon collinear libration points using continuous and impulsive maneuvers

In this study the gravitational perturbations of the Sun and other planets are modeled on the dynamics near the Earth–Moon Lagrange points and optimal continuous and discrete station-keeping maneuvers are found to maintain spacecraft about these points. The most critical perturbation effect near the L₁ and L₂ Lagrange points of the Earth–Moon is the ellipticity of the Moon’s orbit and the Sun’s gravity, respectively. These perturbations deviate the spacecraft from its nominal orbit and have been modeled through a restricted five-body problem (R5BP) formulation compatible with circular restricted three-body problem (CR3BP). The continuous control or impulsive maneuvers can compensate the deviation and keep the spacecraft on the closed orbit about the Lagrange point. The continuous control has been computed using linear quadratic regulator (LQR) and is compared with nonlinear programming (NP). The multiple shooting (MS) has been used for the computation of impulsive maneuvers to keep the trajectory closed and subsequently an optimized MS (OMS) method and multiple impulses optimization (MIO) method have been introduced, which minimize the summation of multiple impulses. In these two methods the spacecraft is allowed to deviate from the nominal orbit; however, the spacecraft trajectory should close itself. In this manner, some closed or nearly closed trajectories around the Earth–Moon Lagrange points are found that need almost zero station-keeping maneuver.     

Modelling the effects of microgravity on the permeability of air interface respiratory epithelial cell layers

Although it has been suggested that microgravity might affect drug absorption in vivo, drug permeability across epithelial barriers has not yet been investigated in vitro during modelled microgravity. Therefore, a cell culture/diffusion chamber was designed specifically to accommodate epithelial cell layers in a 3D-clinostat and allow epithelial permeability to be measured under microgravity conditions in vitro with minimum alteration to established cell culture techniques. Human respiratory epithelial Calu-3 cell layers were used to model the airway epithelium. Cells grown at an air interface in the diffusion chamber from day 1 or day 5 after seeding on 24-well polyester Transwell cell culture inserts developed a similar transepithelial electrical resistance (TER) to cells cultured in conventional cell culture plates. Confluent Calu-3 layers exposed to modelled microgravity in the 3D-clinostat for up to 48 h maintained their high TER. The permeability of the paracellular marker ¹⁴C-mannitol was unaffected after a 24 h rotation of the cell layers in the 3D-clinostat, but was increased 2-fold after 48 h of modelled microgravity. It was demonstrated that the culture/diffusion chamber developed is suitable for culturing epithelial cell layers and, when subjected to rotation in the 3D-clinostat, will be a valuable in vitro system in which to study the influence of microgravity on epithelial permeability and drug transport.     

空间站微重力流体实验设备需求分析

对国际空间站和中国科学实验卫星及载人飞行器上开展的微重力流体实验情况进行论述和分析,重点分析了国际空间站（ISS）微重力流体科学实验设备情况.根据中国空间微重力流体物理科学发展需求,结合国际空间站微重力流体科学实验对设备的需求,提出了未来在中国空间站开展微重力流体实验时空间实验设备需要重点考虑和解决的问题,同时提出相关设计建议.      

SJ-10卫星固体材料燃烧实验装置

为对微重力条件下固体材料着火和火焰传播特性进行研究,研制了实践十号（SJ-10）卫星固体材料燃烧实验装置.利用空间高真空条件,采用实验段内气体环境更新和控制技术,实现了在有限实验空间内对多个实验样品进行研究,并提供准确可控的实验环境条件（氧气浓度和气流速度）.通过地面试验验证,该装置可通过实验样品、氧气浓度、气流速度、点火方式等实验参数的灵活组合,实现空间实验机会的充分利用和预定科学目标.      

Advances of Microgravity Sciences

Advances of microgravity sciences in China are introduced. The research works include ground-based study and space experiments. In the recent years, the main means still are theoretical analysis, numerical simulation, ground-based experiment, and short-time microgravity experiments of drop tower. Besides, many space experiment projects are arranged. SJ-10 recoverable satellite will carry out 19 scientific experiment projects. Nine of them are for microgravity Sciences. The other ways for space microgravity experiment are with the help of Chinese Shenzhou spacecraft, Chinese Tiangong space laboratory, and Chinese space station in the near future. The Chinese space station will become main platform of Chinese microgravity sciences experiment in space.      

Spaceflight results in increase of thick filament but not thin filament proteins in the paramyosin mutant of Caenorhabditis elegans

We have investigated the effect of microgravity during spaceflight on body-wall muscle fiber size and muscle proteins in the paramyosin mutant of Caenorhabditis elegans. Both mutant and wild-type strains were subjected to 10 days of microgravity during spaceflight and compared to ground control groups. No significant change in muscle fiber size or quantity of the protein was observed in wild-type worms; where as atrophy of body-wall muscle and an increase in thick filament proteins were observed in the paramyosin mutant unc-15(e73) animals after spaceflight. We conclude that the mutant with abnormal muscle responded to microgravity by increasing the total amount of muscle protein in order to compensate for the loss of muscle function.     

空间微重力组合体及其控制

空间微重力组合体是绳系卫星系统的升级版,有更强大的功能和应用前景。可是在无控状态下,新旧两款系统的留位（系绳伸出长度不变）和回收（系绳回卷收缩）运动都不稳定,必须置入有效的控制算法才能使其稳定运行。从绳系卫星的简化动力学模型入手,抓住微重力和动态运动中的惯性力相互关系,推导出基础型和扩展型的距离速率控制算法,简明有效地抑制这些系统的不稳定运动和改进空间组合体在轨交会对接控制技术。该算法也可应用于有分布质量和弹性变形的连续系绳的动力学模型以及其他更复杂的绳系卫星（家族）系统的控制中。     

空间引力红移实验的原理与精度

通过对文献[4, 5]关于空间引力红移实验原理与精度的分析,根据爱因斯坦惯性力与引力等效的原理,提出在航天器内部,重力的大部分被惯性力抵消,因而其中的微重力比轨道重力小很多(失重).因此,应当把星载原子钟的重力势取为与微重力相当的有效重力势,而不能简单地将星载钟的重力势取为轨道重力势.另外,检验相对论红移需要将理论值与实验值进行对比,这两种数值均具有误差,而检验精度取决于误差较大者.因此,如果不提高地球重力模型(例如EGM2008)精度而只提高测量精度则不能提高检验精度.     

Biomass recycle as a means to improve the energy efficiency of CELSS algal culture systems.

Algal cultures can be very rapid and efficient means to generate biomass and regenerate the atmosphere for closed environmental life support systems. However, as in the case of most higher plants, a significant fraction of the biomass produced by most algae cannot be directly converted to a useful food product by standard food technology procedures. This waste biomass will serve as an energy drain on the overall system unless it can be efficiently recycled without a significant loss of its energy content. We report experiments in which cultures of the algae Scenedesmus obliquus were grown in the light and at the expense of an added carbon source, which either replaced or supplemented the actinic light. As part of these experiments we tested hydrolyzed waste biomass from these same algae to determine whether the algae themselves could be made part of the biological recycling process. Results indicate that hydrolyzed algal (and plant) biomass can serve as carbon and energy sources for the growth of these algae, suggesting that the efficiency of the closed system could be significantly improved using this recycling process.     

Formation control using μ-synthesis for Inner-Formation Gravity Measurement Satellite System

Inner-Formation Gravity Measurement Satellite System (IFGMSS) is used to map the gravity field of Earth. The IFGMSS consists of two satellites in which one is called “inner satellite” and the other one is named as “outer satellite”. To measure the pure Earth gravity, the inner satellite is located in the cavity of the outer satellite. Because of the shield effect of the cavity, the inner satellite is affected only by the gravitational force, so it can sense Earth gravity precisely. To avoid the collision between the inner satellite and the outer satellite, it is best to perform a real-time control on the outer satellite. In orbit, the mass of the outer satellite decreases with the consumption of its propellant. The orbit angular rate of the inner satellite varies with time due to various disturbing forces. These two parameters’ uncertainties make the C–W function be not so accurate to describe the formation behavior of these two satellites. Furthermore, the thrusters also have some uncertainties due to the unmodelled dynamics. To cancel the effects caused by the above uncertainties, we have studied the robust control method based on the μ-synthesis. This μ-synthesis eliminates the conservativeness and improves the control efficiency comparing with the H_∞ method. Finally, to test the control method, we simulate an IFGMSS mission in which the satellite runs in a sun synchronous circular orbit with an altitude of 300 km. The simulation results show the effectiveness of the robust control method. The performances of the closed-loop system with the μ-controller are tested by the μ-analysis. It has found that the nominal performance, the robust stability and the robust performance are all achieved. The transient simulation results further prove the control response is fast and the accuracy of the relative position meets the demand of the gravity measurement.     

The postmitotic Saccharomyces cerevisiae after spaceflight showed higher viability

The budding yeast Saccharomyces cerevisiae has been proposed as an ideal model organism for clarifying the biological effects caused by spaceflight conditions. The postmitotic S. cerevisiae cells onboard Practice eight recoverable satellite were subjected to spaceflight for 15 days. After recovery, the viability, the glycogen content, the activities of carbohydrate metabolism enzymes, the DNA content and the lipid peroxidation level in yeast cells were analyzed. The viability of the postmitotic yeast cells after spaceflight showed a three-fold increase as compared with that of the ground control cells. Compared to the ground control cells, the lipid peroxidation level in the spaceflight yeast cells markedly decreased. The spaceflight yeast cells also showed an increase in G2/M cell population and a decrease in Sub-G1 cell population. The glycogen content and the activities of hexokinase and succinate dehydrogenase significantly decreased in the yeast cells after spaceflight. In contrast, the activity of malate dehydrogenase showed an obvious increase after spaceflight. These results suggested that microgravity or spaceflight could promote the survival of postmitotic S. cerevisiae cells through regulating carbohydrate metabolism, ROS level and cell cycle progression.     

基于策略的遥感卫星管控方法研究

针对遥感卫星管理控制中业务应用与底层调度紧耦合导致的操作复杂、人工干预多等问题，提出一种基于策略的遥感卫星管控方法。在搭建策略管控模型的基础上，采用分层控制闭环结构，构建卫星控制闭环；提出卫星集群分域管理方法，并设置策略部署机制；详细论述了基于策略的遥感卫星管控实现方法；以海上失联舰船搜救任务为典型案例进行仿真，验证该方法可以完成异构卫星集群基于事件驱动的闭环控制。基于策略的管理方法能够有效降低遥感卫星管控业务的复杂度，实现遥感卫星集群的自动控制。     

Nostoc sphaeroides Kützing,an excellent candidate producer for CELSS

Some phytoplankton can be regarded as possible candidates in the establishment of Controlled Ecological Life Support System (CELSS) for some intrinsic characteristics, the first characteristic is that they should grow rapidly, secondly, they should be able to endure some stress factors and develop some corresponding adaptive strategies; also it is very important that they could provide food rich in nutritious protein and vitamins for the crew; the last but not the least is they can also fulfill the other main functions of CELSS, including supplying oxygen, removing carbon dioxide and recycling the metabolic waste. According to these characteristics, Nostoc sphaeroides, a potential healthy food in China, was selected as the potential producer in CELSS. It was found that the oxygen average evolution rate of this algae is about 150 μmol O₂ mg⁻¹ h⁻¹, and the size of them are ranged from 2 to 20 mm. Also it can be cultured with high population density, which indicated that the potential productivity of Nostoc sphaeroides is higher than other algae in limited volume. We measured the nutrient contents of the cyanobacterium and concluded it was a good food for the crew. Based on above advantages, Nostoc sphaeroides was assumed to a suitable phytoplankton for the establishment of Controlled Ecological Life Support System. We plan to develop suitable bioreactor with the cyanobacterium for supplying oxygen and food in future space missions.     

卫星搭载导线着火前期特性实验研究

导线绝缘层的过热及由此发生的着火和燃烧是载人航天飞行器中引起火灾的主要原因.研究微重力下导线绝缘层的可燃性对于预防飞船中火灾的发生有重要意义.本文描述了SJ-8卫星搭载实验项目导线着火前期特性硬件研制以及实验情况.实验中获得了导线着火前期的温度和辐射特性.在搭载实验中,研究了导线过载电流和导线捆绑方式对导线绝缘层着火前期特性的影响.同时将微重力下的实验结果与地面功能模拟的实验结果进行了对比.实验结果表明,微重力环境下自然对流基本消失,导线和电子电气元件的散热情况恶化,在过载的情况下,就可能过热,从而导致失火.搭载实验中导线先期着火征兆的获得,将为开发我国微重力下的着火监测和早期报警装置提供新的技术途径,为我国载人航天飞行器内安全防火的工程技术工作提供科学依据和基础数据资料.      

A guidance approach to satellite formation reconfiguration based on convex optimization and genetic algorithms

This paper presents a new approach for autonomous reconfiguration of distributed space systems, which ensures safe guidance of spacecraft formations towards the desired patterns while optimizing the total propellant consumption. The orbital transfer is reduced to the form of a convex optimization problem to guarantee rapid computation of control laws. Hence, tasks are iteratively assigned to the component platforms to detect the best reconfiguration strategy. The path-planning is entrusted to a reference satellite of the cluster, that coordinates the remaining ones by means of a procedure based on genetic algorithms. Two methods are proposed, depending on the organizational architecture of the spacecraft formation. In the first one, the maneuver is completely planned by the reference satellite, that determines final tasks and control actions for the whole cluster. As an alternative to such a fully-centralized approach, a distributed version of the algorithm is proposed: tasks are sorted by the reference satellite and transfer orbits are computed by exploiting the computational resources of the whole cluster. Whatever the considered framework, both the planners ensure a safe transition of the formation towards the target geometry. Simulation results show that, when relative distances are of the order of hundreds of meters, a mean delta-v per satellite of the order of 0.1 m/s is required to reconfigure LEO clusters within one orbital period.     

Far-UVC light as a new tool to reduce microbial burden during spacecraft assembly

This work aims to investigate far-UVC light at 222 nm as a new microbial reduction tool for planetary protection purposes which could potentially be integrated into the spacecraft assembly process. The major advantage of far-UVC (222 nm) compared to traditional germicidal UVC (254 nm) is the potential for application throughout the spacecraft assembly process in the presence of humans without adverse health effects due to the limited penetration of far-UVC light into biological materials. Testing the efficacy of 222-nm light at inactivating hardy bacterial cells and spores isolated from spacecraft and associated surfaces is a necessary step to evaluate this technology. We assessed survival of Bacillus pumilus SAFR-032 and Acinetobacter radioresistens 50v1 exposed to 222-nm light on proxy spacecraft surfaces simulated by drying the bacteria on aluminum coupons. The survival fraction of both bacteria followed a single stage decay function up to 60 mJ/cm², revealing similar susceptibility of both species to 222-nm light, which was independent of the exposure rate. Irradiation with far-UVC light at 222 nm is an effective method to decontaminate the proxy spacecraft materials tested in this study.