Simulation experiments of the effect of space environment on bacteriophage and DNA thin films.

The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy. To provide experimental evidence for the hypothesis that interplanetary transfer of the genetic material is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (lambda=254 nm) and high vacuum (10(-4) Pa). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.     

Modelling of Spall in an Anisotropic Aluminium Alloy

Spall caused by hypervelocity impacts at the lower range of velocities could result in significant damage to spacecraft. A number of polycrystalline alloys, used in spacecraft manufacturing, exhibit a pronounced anisotropy in their mechanical properties. The aluminium alloy AA 7010, whose orthotropy is a consequence of the meso-scale phase distribution or grain morphology, has been chosen for this investigation. The material failure observed in plate impact was simulated using a number of spall models. The Hugoniot elastic limit and spall strength have been studied as a function of orientation, and compared to experimental results.     

Ground and on-orbit command and data handling architectures for the Active Rack Isolation System microgravity flight experiment

The Active Rack Isolation System [ARIS] International Space Station [ISS] Characterization Experiment, or ARIS-ICE for short, is a long duration microgravity characterization experiment aboard the ISS. The objective of the experiment is to fully characterize active microgravity performance of the first ARIS rack deployed on the ISS. Efficient ground and on-orbit command and data handling [C&DH] segments are the crux in achieving the challenging objectives of the mission. The objective of the paper is to provide an overview of the C&DH architectures developed for ARIS-ICE, with the view that these architectures may serve as a model for future ISS microgravity payloads. Both ground and on-orbit segments, and their interaction with corresponding ISS C&DH systems are presented. The heart of the on-orbit segment is the ARIS-ICE Payload On-orbit Processor, ARIS-ICE POP for short. The POP manages communication with the ISS C&DH system and other ISS subsystems and payloads, enables automation of test/data collection sequences, and provides a wide range of utilities such as efficient file downlinks/uplinks, data post-processing, data compression and data storage. The hardware and software architecture of the POP is presented and it is shown that the built-in functionality helps to dramatically streamline the efficiency of on-orbit operations. The ground segment has at its heart special ARIS-ICE Ground Support Equipment [GSE] software developed for the experiment. The software enables efficient command and file uplinks, and reconstruction and display of science telemetry packets. The GSE software architecture is discussed along with its interactions with ISS ground C&DH elements. A test sequence example is used to demonstrate the interplay between the ground and on-orbit segments.     

High Excitation ISM and Gas

An overview is given of ISO results on regions of high excitation ISM and gas, i.e. HII regions, the Galactic Centre and Supernova Remnants. IR emission due to fine-structure lines, molecular hydrogen, silicates, polycyclic aromatic hydrocarbons and dust are summarised, their diagnostic capabilities illustrated and their implications highlighted. Based on observations with ISO, an ESA project with instruments funded by ESA Member States (especially the PI countries: France, Germany, The Netherlands, and the United Kingdom), and with the participation of ISAS and NASA.     

Deep Impact: Working Properties for the Target Nucleus – Comet 9P/Tempel 1

In 1998, Comet 9P/Tempel 1 was chosen as the target of the Deep Impact mission (A’Hearn, M. F., Belton, M. J. S., and Delamere, A., Space Sci. Rev., 2005) even though very little was known about its physical properties. Efforts were immediately begun to improve this situation by the Deep Impact Science Team leading to the founding of a worldwide observing campaign (Meech et al., Space Sci. Rev., 2005a). This campaign has already produced a great deal of information on the global properties of the comet’s nucleus (summarized in Table I) that is vital to the planning and the assessment of the chances of success at the impact and encounter. Since the mission was begun the successful encounters of the Deep Space 1 spacecraft at Comet 19P/Borrelly and the Stardust spacecraft at Comet 81P/Wild 2 have occurred yielding new information on the state of the nuclei of these two comets. This information, together with earlier results on the nucleus of comet 1P/Halley from the European Space Agency’s Giotto, the Soviet Vega mission, and various ground-based observational and theoretical studies, is used as a basis for conjectures on the morphological, geological, mechanical, and compositional properties of the surface and subsurface that Deep Impact may find at 9P/Tempel 1. We adopt the following working values (circa December 2004) for the nucleus parameters of prime importance to Deep Impact as follows: mean effective radius = 3.25± 0.2 km, shape – irregular triaxial ellipsoid with a/b = 3.2± 0.4 and overall dimensions of ∼14.4 × 4.4 × 4.4 km, principal axis rotation with period = 41.85± 0.1 hr, pole directions (RA, Dec, J2000) = 46± 10, 73± 10 deg (Pole 1) or 287± 14, 16.5± 10 deg (Pole 2) (the two poles are photometrically, but not geometrically, equivalent), Kron-Cousins (V-R) color = 0.56± 0.02, V-band geometric albedo = 0.04± 0.01, R-band geometric albedo = 0.05± 0.01, R-band H(1,1,0) = 14.441± 0.067, and mass ∼7×10¹³ kg assuming a bulk density of 500 kg m⁻³. As these are working values, {i.e.}, based on preliminary analyses, it is expected that adjustments to their values may be made before encounter as improved estimates become available through further analysis of the large database being made available by the Deep Impact observing campaign. Given the parameters listed above the impact will occur in an environment where the local gravity is estimated at 0.027–0.04 cm s⁻² and the escape velocity between 1.4 and 2 m s⁻¹. For both of the rotation poles found here, the Deep Impact spacecraft on approach to encounter will find the rotation axis close to the plane of the sky (aspect angles 82.2 and 69.7 deg. for pole 1 and 2, respectively). However, until the rotation period estimate is substantially improved, it will remain uncertain whether the impactor will collide with the broadside or the ends of the nucleus.     

The Solar and Cosmic-Ray Synodic Periodicity (1969–1998)

The synodic recurrence of the Mt. Wilson plage index (MPSI) and the Calgary cosmic ray (CR) intensity is investigated, using the wavelet power spectra in the range of 18–38 days, during the last three solar cycles. The unique temporal coincidence between the quasi–synodic MPSI and the CR periods is detected in 1978–1982 (the 21st solar cycle). In the 22nd cycle there is a very strong MPSI synodic recurrence, from 1989.5 to 1990.5, but it is absent in the CR data. In 1992.5–1993.5 the MPSI and CR recurrence phenomenon is in good accordance with the solar wind speed and cosmic ray modulation as measured during the first Ulysses passage around the Sun. The Gnevyshev gap is present in the 27-day recurrence of CR, in agreement with Kudela et al. (1999). This revised version was published online in August 2006 with corrections to the Cover Date.     

Early development of fern gametophytes in microgravity.

Dormant spores of the fern Ceratopteris richardii were flown on Shuttle mission STS-93 to evaluate the effects of micro-g on their development and on their pattern of gene expression. Prior to flight the spores were sterilized and sown into one of two environments: (1) Microscope slides in a video-microscopy module; and (2) Petri dishes. All spores were then stored in darkness until use. Spore germination was initiated on orbit after exposure to light. For the spores on microscope slides, cell level changes were recorded through the clear spore coat of the spores by video microscopy. After their exposure to light, spores in petri dishes were frozen in orbit at four different time points during which on earth gravity fixes the polarity of their development. Spores were then stored frozen in Biological Research in Canister units until recovery on earth. The RNAs from these cells and from 1-g control cells were extracted and analyzed on earth after flight to assay changes in gene expression. Video microscopy results revealed that the germinated spores developed normally in microgravity, although the polarity of their development, which is guided by gravity on earth, was random in space. Differential Display-PCR analyses of RNA extracted from space-flown cells showed that there was about a 5% change in the pattern of gene expression between cells developing in micro-g compared to those developing on earth.     

Annual solar UV exposure and biological effective dose rates on the Martian surface.

The ultraviolet (UV) environment of Mars has been investigated to gain an understanding of the variation of exposure throughout a Martian year, and link this flux to biological effects and possible survival of organisms at the Martian surface. To gain an idea of how the solar UV radiation varies between different regions, including planned landing sites of two future Mars surface missions, we modelled the total solar UV surface flux throughout one Martian year for two different dust scenarios. To understand the degree of solar UV stress on micro-organisms and/or molecules essential for life on the surface of Mars, we also calculated the biologically effective dose (BED) for T7 and Uracil in relevant wavelength regions at the Martian surface as a function of season and latitude, and discuss the biological survival rates in the presence of Martian solar UV radiation. High T7/Uracil BED ratios indicate that even at high latitudes where the UV flux is significantly reduced, the radiation environment is still hostile for life due to the persisting UV-C component of the flux.     

Scanning with charge-coupled devices

The 0.91-m Spacewatch Telescope of the Steward Observatory of the University of Arizona on Kitt Peak is dedicated to scanning with charge-coupled devices (CCDs) during the dark half of the month. We explored six modes of using CCDs for searches of gamma-ray bursters, debris in geosynchronous space, satellites of asteroids, brown dwarfs, the tenth planet, comets, cometesimals, and various types of asteroids. In the process, we gained experience with cosmic rays and artifacts in CCD observations. Each of these topics is described. I especially note that the existence of cometesimals has not been confirmed by the Spacewatch Telescope, contrary to reports published by others.This paper describes a new discipline in astrophysics, scannerscopy, of surveying with a CCD rather than with photography at a Schmidt telescope. It uses the CCD in scanning rather than in sequencing of stare exposures as is done at most observatories. This may save telescope time, and flat-fielding is rarely needed. Usually we turn the drive off, but the scanning can be done with the telescope moving. In any case, the motion on the sky is precisely followed by slaving the charge transfer of the CCD to the drift rate of the image, while the CCD is read out continuously during the observing. Our primary application of CCD scanning is on moving objects such as comets and asteroids. We also do routine astronomy with CCDs, in a transit method, and this yields a precision of better than ±0.7 arc sec.We presently use a Tektronix 2048×2048 CCD, 38 arc min wide, to a limiting magnitude ofV=20.5 (6 detection). This is successful even for discovering rare and small near-Earth asteroids. 1990 UN with a diameter of 90 m and 1991 BA at 9 m are the smallest natural objects observed outside the Earth's atmosphere to date. In a month with good conditions we find typically 2000 new main-belt asteroids and, on average, nearly two near-Earth asteroids. Only the latter are followed up with astrometry. The goal is to study magnitude-frequency relations, as well as to complete the inventory of dangerous impactors on Earth. We are designing a new CCD-scanning telecope to become an order-of-magnitude more effective in the discovery of elusive objects than the Spacewatch Telescope. The paper also describes possibilities with cameras on spacecraft that pass through the asteroid belt; thousands of small asteroids can be observed with the CCDs of CRAF and CASSINI.