An exit cavity was crucial to the polymerase activity of the early ribosome

The emergence of an RNA entity capable of synthesizing peptides was a key prebiotic development. It is hypothesized that a precursor of the modern ribosomal exit tunnel was associated with this RNA entity (e.g., "protoribosome" or "bonding entity") from the earliest time and played an essential role. Various compounds that can bind and activate amino acids, including extremely short RNA chains carrying amino acids, and possibly di- or tripeptides, would have associated with the internal cavity of the protoribosome. This cavity hosts the site for peptide bond formation and adjacent to it a relatively elongated feature that could have evolved to the modern ribosomal exit tunnel, as it is wide enough to allow passage of an oligopeptide. When two of the compounds carrying amino acids or di- or tripeptides (to which we refer, for simplicity, as small aminoacylated RNAs) were in proximity within the heart of the protoribosome, a peptide bond could form spontaneously. The growing peptide would enter the nearby cavity and would not disrupt the attachment of the substrates to the protoribosome or interfere with the subsequent attachment of additional small aminoacylated RNAs. Additionally, the presence of the peptide in the cavity would increase the lifetime of the oligopeptide in the protoribosome. Thus, subsequent addition of another amino acid would be more likely than detachment from the protoribosome, and synthesis could continue. The early ability to synthesize peptides may have resulted in an abbreviated RNA World.     

Medium Earth Orbit dynamical survey and its use in passive debris removal

The Medium Earth Orbit (MEO) region hosts satellites for navigation, communication, and geodetic/space environmental science, among which are the Global Navigation Satellites Systems (GNSS). Safe and efficient removal of debris from MEO is problematic due to the high cost for maneuvers needed to directly reach the Earth (reentry orbits) and the relatively crowded GNSS neighborhood (graveyard orbits). Recent studies have highlighted the complicated secular dynamics in the MEO region, but also the possibility of exploiting these dynamics, for designing removal strategies. In this paper, we present our numerical exploration of the long-term dynamics in MEO, performed with the purpose of unveiling the set of reentry and graveyard solutions that could be reached with maneuvers of reasonable $\mathrm{\Delta }V$ cost. We simulated the dynamics over 120–200?years for an extended grid of millions of fictitious MEO satellites that covered all inclinations from 0 to 90°, using non-averaged equations of motion and a suitable dynamical model that accounted for the principal geopotential terms, 3rd-body perturbations and solar radiation pressure (SRP). We found a sizeable set of usable solutions with reentry times that exceed $～40$ years, mainly around three specific inclination values: 46°, 56°, and 68°; a result compatible with our understanding of MEO secular dynamics. For $\mathrm{\Delta }V?300$ m/s (i.e., achieved if you start from a typical GNSS orbit and target a disposal orbit with $e<0.3$), reentry times from GNSS altitudes exceed $～70$ years, while low-cost ($\mathrm{\Delta }V?5–35$ m/s) graveyard orbits, stable for at lest 200?years, are found for eccentricities up to $e\approx 0.018$. This investigation was carried out in the framework of the EC-funded “ReDSHIFT” project.     

Toward optimizing lighting as a countermeasure to sleep and circadian disruption in space flight

Light is being used as a pre-launch countermeasure to circadian and sleep disruption in astronauts. The effect of light on the circadian system is readily monitored by measurement of plasma melatonin. Our group has established an action spectrum for human melatonin regulation and determined the region of 446-477 nm to be the most potent for suppressing plasma melatonin. The aim of this study was to compare the efficacy of 460 and 555 nm for suppressing melatonin using a within-subjects design. Subjects (N=12) were exposed to equal photon densities (7.18 x 10(12) photons/cm2/s) at 460 and 555 nm. Melatonin suppression was significantly stronger at 460 nm (p<0.02). An extension to the action spectrum showed that 420 nm light at 16 and 32 microW/cm2 significantly suppressed melatonin (p<0.04 and p<0.002). These studies will help optimize lighting countermeasures to circadian and sleep disruption during spaceflight.     

Three Years of Progress in Integrated Diagnostics

In 1983 the NSIA sponsored the first Integrated Diagnostics National Conference. The major finding from this Conference was the lack of integration of those technologies which are part of the diagnostic process. Since that time, much has been done to implement the concept of integrated diagnostics. The NSIA has produced a baseline report on the implementation of the concept. This was followed by a series of Service programs aimed at implementation. The Office of the Secretary of Defense considers solutions to the diagnostic problem to be one of its major R&D goals. This paper discusses the progress made during the last three years on the implementation of integrated diagnostics. The paper also discusses the author's ideas on where the program is going.     

The Pluto Energetic Particle Spectrometer Science Investigation (PEPSSI) on the New Horizons Mission

The Pluto Energetic Particle Spectrometer Science Investigation (PEPSSI) comprises the hardware and accompanying science investigation on the New Horizons spacecraft to measure pick-up ions from Pluto’s outgassing atmosphere. To the extent that Pluto retains its characteristics similar to those of a “heavy comet” as detected in stellar occultations since the early 1980s, these measurements will characterize the neutral atmosphere of Pluto while providing a consistency check on the atmospheric escape rate at the encounter epoch with that deduced from the atmospheric structure at lower altitudes by the ALICE, REX, and SWAP experiments on New Horizons. In addition, PEPSSI will characterize any extended ionosphere and solar wind interaction while also characterizing the energetic particle environment of Pluto, Charon, and their associated system. First proposed for development for the Pluto Express mission in September 1993, what became the PEPSSI instrument went through a number of development stages to meet the requirements of such an instrument for a mission to Pluto while minimizing the required spacecraft resources. The PEPSSI instrument provides for measurements of ions (with compositional information) and electrons from 10 s of keV to ～1 MeV in a 160°×12° fan-shaped beam in six sectors for 1.5 kg and ～2.5 W.     

Chromosome aberrations in human lymphocytes induced by 250 MeV protons: effects of dose, dose rate and shielding.

Although the space radiation environment consists predominantly of energetic protons, astronauts inside a spacecraft are chronically exposed to both primary particles as well as secondary particles that are generated when the primary particles penetrate the spacecraft shielding. Secondary neutrons and secondary charged particles can have an LET value that is greater than the primary protons and, therefore, produce a higher relative biological effectiveness (RBE). Using the accelerator facility at Loma Linda University, we exposed human lymphocytes in vitro to 250 MeV protons with doses ranging from 0 to 60 cGy at three different dose rates: a low dose rate of 7.5 cGy/h, an intermediate dose rate of 30 cGy/h and a high dose rate of 70 cGy/min. The effect of 15 g/cm2 aluminum shielding on the induction of chromosome aberrations was investigated for each dose rate. After exposure, lymphocytes were incubated in growth medium containing phytohemagglutinin (PHA) and chromosome spreads were collected using a chemical-induced premature chromosome condensation (PCC) technique. Aberrations were analyzed using the fluorescence in situ hybridization (FISH) technique with three different colored chromosome-painting probes. The frequency of reciprocal and complex-type chromosome exchanges were compared in shielded and unshielded samples.     

Giant Planet Ionospheres and Thermospheres: The Importance of Ion-Neutral Coupling

Planetary upper atmospheres-coexisting thermospheres and ionospheres-form an important boundary between the planet itself and interplanetary space. The solar wind and radiation from the Sun may react with the upper atmosphere directly, as in the case of Venus. If the planet has a magnetic field, however, such interactions are mediated by the magnetosphere, as in the case of the Earth. All of the Solar System’s giant planets have magnetic fields of various strengths, and interactions with their space environments are thus mediated by their respective magnetospheres. This article concentrates on the consequences of magnetosphere-atmosphere interactions for the physical conditions of the thermosphere and ionosphere. In particular, we wish to highlight important new considerations concerning the energy balance in the upper atmosphere of Jupiter and Saturn, and the role that coupling between the ionosphere and thermosphere may play in establishing and regulating energy flows and temperatures there. This article also compares the auroral activity of Earth, Jupiter, Saturn and Uranus. The Earth’s behaviour is controlled, externally, by the solar wind. But Jupiter’s is determined by the co-rotation or otherwise of the equatorial plasmasheet, which is internal to the planet’s magnetosphere. Despite being rapid rotators, like Jupiter, Saturn and Uranus appear to have auroral emissions that are mainly under solar (wind) control. For Jupiter and Saturn, it is shown that Joule heating and “frictional” effects, due to ion-neutral coupling can produce large amounts of energy that may account for their high exospheric temperatures.     

The James Webb Space Telescope

The James Webb Space Telescope (JWST) is a large (6.6 m), cold (<50 K), infrared (IR)-optimized space observatory that will be launched early in the next decade into orbit around the second Earth–Sun Lagrange point. The observatory will have four instruments: a near-IR camera, a near-IR multiobject spectrograph, and a tunable filter imager will cover the wavelength range, 0.6 < ; < 5.0 μ m, while the mid-IR instrument will do both imaging and spectroscopy from 5.0 < ; < 29 μ m.The JWST science goals are divided into four themes. The key objective of The End of the Dark Ages: First Light and Reionization theme is to identify the first luminous sources to form and to determine the ionization history of the early universe. The key objective of The Assembly of Galaxies theme is to determine how galaxies and the dark matter, gas, stars, metals, morphological structures, and active nuclei within them evolved from the epoch of reionization to the present day. The key objective of The Birth of Stars and Protoplanetary Systems theme is to unravel the birth and early evolution of stars, from infall on to dust-enshrouded protostars to the genesis of planetary systems. The key objective of the Planetary Systems and the Origins of Life theme is to determine the physical and chemical properties of planetary systems including our own, and investigate the potential for the origins of life in those systems. Within these themes and objectives, we have derived representative astronomical observations.To enable these observations, JWST consists of a telescope, an instrument package, a spacecraft, and a sunshield. The telescope consists of 18 beryllium segments, some of which are deployed. The segments will be brought into optical alignment on-orbit through a process of periodic wavefront sensing and control. The instrument package contains the four science instruments and a fine guidance sensor. The spacecraft provides pointing, orbit maintenance, and communications. The sunshield provides passive thermal control. The JWST operations plan is based on that used for previous space observatories, and the majority of JWST observing time will be allocated to the international astronomical community through annual peer-reviewed proposal opportunities.     

Johannes Geiss’ Investigations of Solar, Heliospheric and Interstellar Matter

Johannes Geiss is a world leader and foremost expert on measurements and interpretation of the composition of matter that reveals the history, present state, and future of astronomical objects. With his Swiss team he was first to measure the composition of the noble gases in the solar wind when in the late 1960s he flew the brilliant solar wind collecting foil experiments on the five Apollo missions to the moon. Always at the forefront of the art of composition measurements, he with his colleagues determined the isotopic and elemental composition of the solar wind using instruments characterized by innovative design that have provided the most comprehensive record of the solar wind composition under all solar wind conditions at all helio-latitudes. He discovered heavy interstellar pickup ions, from which the composition of the neutral gas of the Local Interstellar Cloud is determined, and the “Inner Source” of pickup ions. Johannes Geiss played a key role both in the in-situ measurements and modeling of molecular ions in comets, and the interpretation of these data. He and co-workers measured the composition of plasmas in the magnetospheres of Earth and Jupiter. Here we highlight Johannes Geiss’ many discoveries and seminal contributions to our knowledge of the composition of matter of the Sun, solar wind, interstellar gas, early universe, comets and magnetospheres.     

Interstellar and Inner Source Pickup Ions Observed with Swics on Ulysses

Many species of pickup ions, both of interstellar origin and from an inner, distributed source have been discovered using data from the Solar Wind Ion Composition Spectrometer (SWICS) on Ulysses. Velocity distribution functions of these ions were measured for the first time over heliocentric distances between 1.35 and 5.4 AU, both at high and low latitudes, and in the disturbed slow solar wind as well as the steady fast wind of the polar coronal holes. This has given us the first glance at plasma properties of suprathermal ions in various solar wind flows, and is enabling us to study the chemical and, in the case of He, the isotopic composition of the local interstellar cloud. Among the new findings are (a) the surprisingly weak pitch-angle scattering of low rigidity, suprathermal ions leading to strongly anisotropic velocity distributions in radial magnetic fields, (b) the efficient injection and consequent acceleration of pickup ions, especially He+ and H+, in the turbulent solar wind, and (c) the discovery of a new extended source releasing carbon, oxygen, nitrogen and possibly other atoms and molecules in the inner solar system. Pickup ion measurements are now used to study the characteristics of the local interstellar cloud (LIC) and, in particular, to determine accurately the abundance of atomic H, He, N, O, and Ne, the isotopes of He and Ne, as well as the ionization fractions of H and He in the LIC. Pickup ion observations allow us to infer the location of the termination shock and, in combination with measurements of anomalous cosmic rays, to investigate termination shock acceleration mechanisms. This revised version was published online in June 2006 with corrections to the Cover Date.