The Mawrth Vallis region of Mars: A potential landing site for the Mars Science Laboratory (MSL) mission

The primary objective of NASA's Mars Science Laboratory (MSL) mission, which will launch in 2011, is to characterize the habitability of a site on Mars through detailed analyses of the composition and geological context of surface materials. Within the framework of established mission goals, we have evaluated the value of a possible landing site in the Mawrth Vallis region of Mars that is targeted directly on some of the most geologically and astrobiologically enticing materials in the Solar System. The area around Mawrth Vallis contains a vast (>1?×?10? km2) deposit of phyllosilicate-rich, ancient, layered rocks. A thick (>150?m) stratigraphic section that exhibits spectral evidence for nontronite, montmorillonite, amorphous silica, kaolinite, saponite, other smectite clay minerals, ferrous mica, and sulfate minerals indicates a rich geological history that may have included multiple aqueous environments. Because phyllosilicates are strong indicators of ancient aqueous activity, and the preservation potential of biosignatures within sedimentary clay deposits is high, martian phyllosilicate deposits are desirable astrobiological targets. The proposed MSL landing site at Mawrth Vallis is located directly on the largest and most phyllosilicate-rich deposit on Mars and is therefore an excellent place to explore for evidence of life or habitability.     

Mitigation of the impact of terrestrial contamination on organic measurements from the Mars Science Laboratory

The objective of the 2009 Mars Science Laboratory (MSL), which is planned to follow the Mars Exploration Rovers and the Phoenix lander to the surface of Mars, is to explore and assess quantitatively a site on Mars as a potential habitat for present or past life. Specific goals include an assessment of the past or present biological potential of the target environment and a characterization of its geology and geochemistry. Included in the 10 investigations of the MSL rover is the Sample Analysis at Mars (SAM) instrument suite, which is designed to obtain trace organic measurements, measure water and other volatiles, and measure several light isotopes with experiment sequences designed for both atmospheric and solid-phase samples. SAM integrates a gas chromatograph, a mass spectrometer, and a tunable laser spectrometer supported by sample manipulation tools both within and external to the suite. The sub-part-per-billion sensitivity of the suite for trace species, particularly organic molecules, along with a mobile platform that will contain many kilograms of organic materials, presents a considerable challenge due to the potential for terrestrial contamination to mask the signal of martian organics. We describe the effort presently underway to understand and mitigate, wherever possible within the resource constraints of the mission, terrestrial contamination in MSL and SAM measurements.     

好奇心号巡视器及其特点分析

"火星科学实验室"(Mars Science Laboratory,MSL)是NASA于2011年11月26日发射的火星探测器,其上的好奇心号(Curiosity)巡视器已经于2012年8月6日着陆火星;其主要科学目标包括研究火星存在生命的可能性、火星气候特征、火星地质过程,并为将来的载人着陆作准备;经过多次论证,其着陆区为盖尔撞击坑(Gale Crater)。与过去的火星巡视器相比,它携带了更加先进的科学仪器,能够精确分析采集样品的化学成分、光谱特征等;在科学工作小组的指导下,其运行模式包括行走、勘查、接近目标、接触目标与样品分析;通过上述工作,"火星科学实验室"将对火星生命及可居住性进行全面探测。     

Survival and germinability of Bacillus subtilis spores exposed to simulated Mars solar radiation: implications for life detection and planetary protection

Bacterial spores have been considered as microbial life that could survive interplanetary transport by natural impact processes or human spaceflight activity. Deposition of terrestrial microbes or their biosignature molecules onto the surface of Mars could negatively impact life detection experiments and planetary protection measures. Simulated Mars solar radiation, particularly the ultraviolet component, has been shown to reduce spore viability, but its effect on spore germination and resulting production of biosignature molecules has not been explored. We examined the survival and germinability of Bacillus subtilis spores exposed to simulated martian conditions that include solar radiation. Spores of B. subtilis that contain luciferase resulting from expression of an sspB-luxAB gene fusion were deposited on aluminum coupons to simulate deposition on spacecraft surfaces and exposed to simulated Mars atmosphere and solar radiation. The equivalent of 42 min of simulated Mars solar radiation exposure reduced spore viability by nearly 3 logs, while germination-induced bioluminescence, a measure of germination metabolism, was reduced by less than 1 log. The data indicate that spores can retain the potential to initiate germination-associated metabolic processes and produce biological signature molecules after being rendered nonviable by exposure to Mars solar radiation.     

The Dynamic Albedo of Neutrons (DAN) experiment for NASA's 2009 Mars Science Laboratory

We present a summary of the physical principles and design of the Dynamic Albedo of Neutrons (DAN) instrument onboard NASA's 2009 Mars Science Laboratory (MSL) mission. The DAN instrument will use the method of neutron-neutron activation analysis in a space application to study the abundance and depth distribution of water in the martian subsurface along the path of the MSL rover.     

Cave biosignature suites: microbes,minerals, and Mars

Earth's subsurface offers one of the best possible sites to search for microbial life and the characteristic lithologies that life leaves behind. The subterrain may be equally valuable for astrobiology. Where surface conditions are particularly hostile, like on Mars, the subsurface may offer the only habitat for extant lifeforms and access to recognizable biosignatures. We have identified numerous unequivocally biogenic macroscopic, microscopic, and chemical/geochemical cave biosignatures. However, to be especially useful for astrobiology, we are looking for suites of characteristics. Ideally, "biosignature suites" should be both macroscopically and microscopically detectable, independently verifiable by nonmorphological means, and as independent as possible of specific details of life chemistries--demanding (and sometimes conflicting) criteria. Working in fragile, legally protected environments, we developed noninvasive and minimal impact techniques for life and biosignature detection/characterization analogous to Planetary Protection Protocols. Our difficult field conditions have shared limitations common to extraterrestrial robotic and human missions. Thus, the cave/subsurface astrobiology model addresses the most important goals from both scientific and operational points of view. We present details of cave biosignature suites involving manganese and iron oxides, calcite, and sulfur minerals. Suites include morphological fossils, mineral-coated filaments, living microbial mats and preserved biofabrics, 13C and 34S values consistent with microbial metabolism, genetic data, unusual elemental abundances and ratios, and crystallographic mineral forms.     

An astrophysical view of Earth-based metabolic biosignature gases

Microbial life on Earth uses a wide range of chemical and energetic resources from diverse habitats. An outcome of this microbial diversity is an extensive and varied list of metabolic byproducts. We review key points of Earth-based microbial metabolism that are useful to the astrophysical search for biosignature gases on exoplanets, including a list of primary and secondary metabolism gas byproducts. Beyond the canonical, unique-to-life biosignature gases on Earth (O(2), O(3), and N(2)O), the list of metabolic byproducts includes gases that might be associated with biosignature gases in appropriate exoplanetary environments. This review aims to serve as a starting point for future astrophysical biosignature gas research.     

On biogenicity criteria for endolithic microborings on early Earth and beyond

Micron-sized cavities created by the actions of rock-etching microorganisms known as euendoliths are explored as a biosignature for life on early Earth and perhaps Mars. Rock-dwelling organisms can tolerate extreme environmental stresses and are excellent candidates for the colonization of early Earth and planetary surfaces. Here, we give a brief overview of the fossil record of euendoliths in both sedimentary and volcanic rocks. We then review the current understanding of the controls upon the distribution of euendolithic microborings and use these to propose three lines of approach for testing their biogenicity: first, a geological setting that demonstrates a syngenetic origin for the euendolithic microborings; second, microboring morphologies and distributions that are suggestive of biogenic behavior and distinct from ambient inclusion trails; and third, elemental and isotopic evidence suggestive of biological processing. We use these criteria and the fossil record of terrestrial euendoliths to outline potential environments and techniques to search for endolithic microborings on Mars.     

Astrobiology through the ages of Mars: the study of terrestrial analogues to understand the habitability of Mars

Mars has undergone three main climatic stages throughout its geological history, beginning with a water-rich epoch, followed by a cold and semi-arid era, and transitioning into present-day arid and very cold desert conditions. These global climatic eras also represent three different stages of planetary habitability: an early, potentially habitable stage when the basic requisites for life as we know it were present (liquid water and energy); an intermediate extreme stage, when liquid solutions became scarce or very challenging for life; and the most recent stage during which conditions on the surface have been largely uninhabitable, except perhaps in some isolated niches. Our understanding of the evolution of Mars is now sufficient to assign specific terrestrial environments to each of these periods. Through the study of Mars terrestrial analogues, we have assessed and constrained the habitability conditions for each of these stages, the geochemistry of the surface, and the likelihood for the preservation of organic and inorganic biosignatures. The study of these analog environments provides important information to better understand past and current mission results as well as to support the design and selection of instruments and the planning for future exploratory missions to Mars.     

火星进入、下降与着陆技术的新进展——以“火星科学实验室”为例

文章以"火星科学实验室"为例,对火星进入、下降和着陆(EDL)技术的新进展进行了分析和总结。首先,简要介绍了"火星科学实验室"任务需求和遇到的技术挑战;然后,详细说明了相应的对策以及具体技术方案,并针对当前所遇到的问题,列举了一些可能的解决方法;最后,对"火星科学实验室"发展的新技术、新方法进行了总结。     

火星精确着陆制导问题分析与展望

美国火星科学实验室(MSL)任务成功将“好奇”号火星车着陆到火星表面,开创了火星精确着陆探测的新局面。以MSL着陆任务为典型代表,分析了目前火星着陆探测进入、下降和着陆（Entry, Descent and Landing, EDL）过程的制导方案及制导系统的发展趋势。以在火星高海拔、复杂地形区域定点着陆为潜在工程目标,归纳了火星EDL过程面临的制导主要问题。根据未来制导系统自主性和自适应性的技术需求及潜在工程任务制导面临的问题,提出了火星EDL制导方面需要解决的关键技术,并对其在未来工程中的应用潜力进行了展望。     

The Urey instrument: an advanced in situ organic and oxidant detector for Mars exploration

The Urey organic and oxidant detector consists of a suite of instruments designed to search for several classes of organic molecules in the martian regolith and ascertain whether these compounds were produced by biotic or abiotic processes using chirality measurements. These experiments will also determine the chemical stability of organic molecules within the host regolith based on the presence and chemical reactivity of surface and atmospheric oxidants. Urey has been selected for the Pasteur payload on the European Space Agency's (ESA's) upcoming 2013 ExoMars rover mission. The diverse and effective capabilities of Urey make it an integral part of the payload and will help to achieve a large portion of the mission's primary scientific objective: "to search for signs of past and present life on Mars." This instrument is named in honor of Harold Urey for his seminal contributions to the fields of cosmochemistry and the origin of life.     

In situ microbial detection in Mojave Desert soil using native fluorescence

We report on the use of a portable instrument for microbial detection in the Mojave Desert soil and the potential for its use on Mars. The instrument is based on native fluorescence and employs four excitation wavelengths combined with four emission wavelengths. A soil dilution series in which known numbers of Bacillus subtilis spores were added to soil was used to determine the sensitivity of the instrument. We found that the fluorescence of the biological and organic components of the desert soil samples studied can be as strong as the fluorescence of the mineral component of these soils. Using the calibration derived from B. subtilis spores, we estimated that microbial content at our primary sampling site was 10(7) bacteria per gram of soil, a level confirmed by phospholipid fatty acid analysis. At a nearby site, but in a slightly different geological setting, we tested the instrument's ability to map out microbial concentrations in situ. Over a ～50 m diameter circle, soil microbial concentrations determined with the B. subtilis calibration indicate that the concentrations of microorganisms detected varies from 10(4) to 10(7) cells per gram of soil. We conclude that fluorescence is a promising method for detecting soil microbes in noncontact applications in extreme environments on Earth and may have applications on future missions to Mars.     

美国火星表面探测使命述评（上）

从1975年发射（1976年着陆火星）的海盗-1探测器以来,美国已成功执行了6次火星表面探测使命,即海盗-1与海盗-2轨道器/着陆器,＂火星探路者＂（MPF）着陆器/巡游车,＂勇气＂与＂机遇＂火星探测巡游车（MER）,以及＂凤凰＂着陆器;而推迟到2011年发射的＂好奇心＂火星科学实验室（MSL）将火星着陆技术与表面巡游车技术推向一个新的高度。从＂海盗＂着陆器到＂好奇心＂巡游车、美国历经三种火星着陆系统与三代火星表面巡游车技术的发展。三种着陆系统为着陆腿着陆系统（＂海盗＂与＂凤凰＂）,气囊着陆系统（MPF与MER）,以及空中吊机着陆系统（MSL）。三代巡游车为MPF＂旅居者＂巡游车、MER＂勇气＂与＂机遇＂巡游车,以及MSL＂好奇心＂巡游车。现在,美国在火星进入、降落与着陆（EDL）运作与表面避障移动方面,已达到技术成熟与先进的水平,满足安全着陆与表面移动探测的要求。文章阐述美国上述七项火星表面探测使命的立项背景、科学目标与有效载荷、飞行系统组成,以及飞行运作程序;分析美国火星着陆技术与表面巡游车技术的发展。     

美国火星表面探测使命述评（下）

从1975年8月发射（1976年7月着陆火星）的海盗-1探测器以来,美国已成功执行了6次火星表面探测使命,即海盗-1与海盗-2轨道器/着陆器,＂火星探路者＂（MPF）着陆器/巡游车,＂勇气＂与＂机遇＂火星探测巡游车（MER）,以及＂凤凰＂着陆器;而推迟到2011年发射的火星科学实验室（MSL）将火星着陆技术与表面巡游车技术推向一个新的高度。从＂海盗＂着陆器到MSL＂好奇心＂巡游车、美国历经三种火星着陆系统与三代火星表面巡游车技术的发展。三种着陆系统为着陆腿着陆系统（＂海盗＂与＂凤凰＂）,气囊着陆系统（MPF与MER）,以及空中吊机着陆系统（MSL）。三代巡游车为MPF＂旅居者＂巡游车、MER＂勇气＂与＂机遇＂巡游车,以及MSL＂好奇心＂巡游车。现在,美国在火星进入、降落与着陆（EDL）运作与表面避障移动方面,已达到技术成熟与先进的水平,满足安全着陆与表面移动探测的要求。文章阐述美国上述七项火星表面探测使命的立项背景、科学目标与有效载荷、飞行系统组成,以及飞行运作程序;分析美国火星着陆技术与表面巡游车技术的发展。     

The PROCESS experiment: amino and carboxylic acids under Mars-like surface UV radiation conditions in low-earth orbit

The search for organic molecules at the surface of Mars is a top priority of the next Mars exploration space missions: Mars Science Laboratory (NASA) and ExoMars (ESA). The detection of organic matter could provide information about the presence of a prebiotic chemistry or even biological activity on this planet. Therefore, a key step in interpretation of future data collected by these missions is to understand the preservation of organic matter in the martian environment. Several laboratory experiments have been devoted to quantifying and qualifying the evolution of organic molecules under simulated environmental conditions of Mars. However, these laboratory simulations are limited, and one major constraint is the reproduction of the UV spectrum that reaches the surface of Mars. As part of the PROCESS experiment of the European EXPOSE-E mission on board the International Space Station, a study was performed on the photodegradation of organics under filtered extraterrestrial solar electromagnetic radiation that mimics Mars-like surface UV radiation conditions. Glycine, serine, phthalic acid, phthalic acid in the presence of a mineral phase, and mellitic acid were exposed to these conditions for 1.5 years, and their evolution was determined by Fourier transform infrared spectroscopy after their retrieval. The results were compared with data from laboratory experiments. A 1.5-year exposure to Mars-like surface UV radiation conditions in space resulted in complete degradation of the organic compounds. Half-lives between 50 and 150?h for martian surface conditions were calculated from both laboratory and low-Earth orbit experiments. The results highlight that none of those organics are stable under low-Earth orbit solar UV radiation conditions.     

Spiders: water-driven erosive structures in the southern hemisphere of Mars

Recent data from space missions reveal that there are ongoing climatic changes and erosive processes that continuously modify surface features of Mars. We have investigated the seasonal dynamics of a number of morphological features located at Inca City, a representative area at high southern latitude that has undergone seasonal processes. By integrating visual information from the Mars Orbiter Camera on board the Mars Global Surveyor and climatic cycles from a Mars' General Circulation Model, and considering the recently reported evidence for the presence of water-ice and aqueous precipitates on Mars, we propose that a number of the erosive features identified in Inca City, among them spiders, result from the seasonal melting of aqueous salty solutions.     

Key science questions from the second conference on early Mars: geologic, hydrologic, and climatic evolution and the implications for life

In October 2004, more than 130 terrestrial and planetary scientists met in Jackson Hole, WY, to discuss early Mars. The first billion years of martian geologic history is of particular interest because it is a period during which the planet was most active, after which a less dynamic period ensued that extends to the present day. The early activity left a fascinating geological record, which we are only beginning to unravel through direct observation and modeling. In considering this time period, questions outnumber answers, and one of the purposes of the meeting was to gather some of the best experts in the field to consider the current state of knowledge, ascertain which questions remain to be addressed, and identify the most promising approaches to addressing those questions. The purpose of this report is to document that discussion. Throughout the planet's first billion years, planetary-scale processes-including differentiation, hydrodynamic escape, volcanism, large impacts, erosion, and sedimentation-rapidly modified the atmosphere and crust. How did these processes operate, and what were their rates and interdependencies? The early environment was also characterized by both abundant liquid water and plentiful sources of energy, two of the most important conditions considered necessary for the origin of life. Where and when did the most habitable environments occur? Did life actually occupy them, and if so, has life persisted on Mars to the present? Our understanding of early Mars is critical to understanding how the planet we see today came to be.     

A concept for NASA's Mars 2016 astrobiology field laboratory

The Mars Program Plan includes an integrated and coordinated set of future candidate missions and investigations that meet fundamental science objectives of NASA and the Mars Exploration Program (MEP). At the time this paper was written, these possible future missions are planned in a manner consistent with a projected budget profile for the Mars Program in the next decade (2007-2016). As with all future missions, the funding profile depends on a number of factors that include the exact cost of each mission as well as potential changes to the overall NASA budget. In the current version of the Mars Program Plan, the Astrobiology Field Laboratory (AFL) exists as a candidate project to determine whether there were (or are) habitable zones and life, and how the development of these zones may be related to the overall evolution of the planet. The AFL concept is a surface exploration mission equipped with a major in situ laboratory capable of making significant advancements toward the Mars Program's life-related scientific goals and the overarching Vision for Space Exploration. We have developed several concepts for the AFL that fit within known budget and engineering constraints projected for the 2016 and 2018 Mars mission launch opportunities. The AFL mission architecture proposed here assumes maximum heritage from the 2009 Mars Science Laboratory (MSL). Candidate payload elements for this concept were identified from a set of recommendations put forth by the Astrobiology Field Laboratory Science Steering Group (AFL SSG) in 2004, for the express purpose of identifying overall rover mass and power requirements for such a mission. The conceptual payload includes a Precision Sample Handling and Processing System that would replace and augment the functionality and capabilities provided by the Sample Acquisition Sample Processing and Handling system that is currently part of the 2009 MSL platform.     

Fluorescence microscopy as a tool for in situ life detection

The identification of extant and, in some cases, extinct bacterial life is most convincingly and efficiently performed with modern high-resolution microscopy. Epifluorescence microscopy of microbial autofluorescence or in conjunction with fluorescent dyes is among the most useful of these techniques. We explored fluorescent labeling and imaging of bacteria in rock and soil in the context of in situ life detection for planetary exploration. The goals were two-fold: to target non-Earth-centric biosignatures with the greatest possible sensitivity and to develop labeling procedures amenable to robotic implementation with technologies that are currently space qualified. A wide panel of commercially available dyes that target specific biosignature molecules was screened, and those with desirable properties (i.e., minimal binding to minerals, strong autofluorescence contrast, no need for wash steps) were identified. We also explored the potential of semiconductor quantum dots (QDs) as bacterial and space probes. A specific instrument for space implementation is suggested and discussed.