Far-UVC light as a new tool to reduce microbial burden during spacecraft assembly

This work aims to investigate far-UVC light at 222 nm as a new microbial reduction tool for planetary protection purposes which could potentially be integrated into the spacecraft assembly process. The major advantage of far-UVC (222 nm) compared to traditional germicidal UVC (254 nm) is the potential for application throughout the spacecraft assembly process in the presence of humans without adverse health effects due to the limited penetration of far-UVC light into biological materials. Testing the efficacy of 222-nm light at inactivating hardy bacterial cells and spores isolated from spacecraft and associated surfaces is a necessary step to evaluate this technology. We assessed survival of Bacillus pumilus SAFR-032 and Acinetobacter radioresistens 50v1 exposed to 222-nm light on proxy spacecraft surfaces simulated by drying the bacteria on aluminum coupons. The survival fraction of both bacteria followed a single stage decay function up to 60 mJ/cm², revealing similar susceptibility of both species to 222-nm light, which was independent of the exposure rate. Irradiation with far-UVC light at 222 nm is an effective method to decontaminate the proxy spacecraft materials tested in this study.     

Water Partitioning in Planetary Embryos and Protoplanets with Magma Oceans

The water content of magma oceans is widely accepted as a key factor that determines whether a terrestrial planet is habitable. Water ocean mass is determined as a result not only of water delivery and loss, but also of water partitioning among several reservoirs. Here we review our current understanding of water partitioning among the atmosphere, magma ocean, and solid mantle of accreting planetary embryos and protoplanets just after giant collisions. Magma oceans are readily formed in planetary embryos and protoplanets in their accretion phase. Significant amounts of water are partitioned into magma oceans, provided the planetary building blocks are water-rich enough. Particularly important but still quite uncertain issues are how much water the planetary building blocks contain initially and how water goes out of the solidifying mantle and is finally degassed to the atmosphere. Constraints from both solar-system explorations and exoplanet observations and also from laboratory experiments are needed to resolve these issues.     

The Role and Contributions of Energetic Neutral Atom (ENA) Imaging in Magnetospheric Substorm Research

Energetic Neutral Atom (ENA) imaging has contributed substantially to substorm research. This technique has allowed significant advances in areas such as observation and quantification of injected particle drift as a function of energy, observation of dynamics in the tail that are directly related to the effects of imposed (growth phase) and induced (expansion phase) electric fields on the plasma, the prompt extraction of oxygen from the ionosphere during substorms, the relationship between storms and substorms, and the timing of substorm ENA signatures. We present discussion of the advantages and shortcomings of the ENA technique for studying space plasmas. Although the technique is in its infancy, it is yielding results that enrich our understanding of the substorm process and its effects.     

JSF动力的最新进展

普惠公司为JSF研制的F135发动机已完成了系统发展和验证的关键设计评审.今年10月将开始发动机的地面试验。GE和罗-罗公司计划为JSF研制的备选发动机F136将在明年7月在GE公司进行首次试验     

Observations of the Acceleration of Polar Solar Wind Near Solar Maximum

In a previous paper (Tappin et al., 1999) we used cross-correlation analysis of high-cadence observations with the LASCO coronagraphs to trace the acceleration of the solar wind at low latitudes. In this paper we present a similar analysis carried out over the North pole of the Sun. The observations which were made in March 2000 with the C3 coronagraph show low bulk flow speeds (comparable to or lower than those seen at the equator in early 1998). We observe the acceleration continuing to the edge of the C3 field of view at about 30 R _⊙. We also observe, as at low latitude, a high-speed tail but now reaching out well beyond 2000 km s⁻¹. We do not see a clear signature of a fast polar bulk flow. We therefore conclude that at this phase of the solar cycle, any fast bulk flow occupies only a small part of the line of sight and is therefore overwhelmed by the denser slow solar wind in these observations. We also show that the fast component is consistent with observed solar wind speeds at 1 AU. This revised version was published online in August 2006 with corrections to the Cover Date.     

The role of DNA repair on cell killing by charged particles.

It can be noted that it is not simple double strand breaks (dsb) but the non-reparable breaks that are associated with high biological effectiveness in the cell killing effect for high LET radiation. Here, we have examined the effectiveness of fast neutrons and low (initial energy = 12 MeV/u) or high (135 MeV/u) energy charged particles on cell death in 19 mammalian cell lines including radiosensitive mutants. Some of the radiosensitive lines were deficient in DNA dsb repair such as LX830, M10, V3, and L5178Y-S cells and showed lower values of relative biological effectiveness (RBE) for fast neutrons if compared with their parent cell lines. The other lines of human ataxia-telangiectasia fibroblasts, irs 1, irs 2, irs 3 and irs1SF cells, which were also radiosensitive but known as proficient in dsb repair, showed moderated RBEs. Dsb repair deficient mutants showed low RBE values for heavy ions. These experimental findings suggest that the DNA repair system does not play a major role against the attack of high linear energy transfer (LET) radiations. Therefore, we hypothesize that a main cause of cell death induced by high LET radiations is due to non-reparable dsb, which are produced at a higher rate compared to low LET radiations.     

Simulation experiments of the effect of space environment on bacteriophage and DNA thin films.

The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy. To provide experimental evidence for the hypothesis that interplanetary transfer of the genetic material is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (lambda=254 nm) and high vacuum (10(-4) Pa). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.     

Properties and performance of extended target motion analysis

The problem of target motion analysis (TMA) has been the subject of an important literature. However, present methods use data estimated by a short time analysis (azimuths, Dopplers, etc.). For far sources, the nonstationarities of the array processing outputs, induced by the sources motion, may be simply modeled. This model leads one to consider directly a spatio-temporal TMA. Then new (spatio-temporal) data can be estimated. These estimates correspond to a long time analysis. Further, note that they are estimated independently of the (classical) bearings. In this general framework, the concept of source trajectory replaces the classical instantaneous bearings. Corresponding TMA algorithms are then studied. Then the study of statistical performance is carefully studied     

Influence of long-term altered gravity on the swimming performance of developing cichlid fish: including results from the 2nd German Spacelab Mission D-2.

This study presents qualitative and quantitative data concerning gravity-dependent changes in the swimming behaviour of developing cichlid fish larvae (Oreochromis mossambicus) after a 9 resp. 10 days exposure to increased acceleration (centrifuge experiments), to reduced gravity (fast-rotating clinostat), changed accelerations (parabolic aircraft flights) and to near weightlessness (2nd German Spacelab Mission D-2). Changes of gravity initially cause disturbances of the swimming performance of the fish larvae. With prolonged stay in orbit a step by step normalisation of the swimming behaviour took place in the fish. After return to 1g earth conditions no somersaulting or looping could be detected concerning the fish, but still slow and disorientated movements as compared to controls occurred. The fish larvae adapted to earth gravity within 3-5 days. Fish seem to be in a distinct early developmental stages extreme sensitive and adaptable to altered gravity; However, elder fish either do not react or show compensatory behaviour e.g. escape reactions.