Physiologic and metabolic responses of wheat seedlings to elevated and super-elevated carbon dioxide

The metabolic consequence of suboptimal (400 μmol mol⁻¹ or ppm), near-optimal (1500 ppm) and supra-optimal (10,000 ppm) atmospheric carbon dioxide concentrations [CO₂] was investigated in an attempt to reveal plausible underlying mechanisms for the differential physiological and developmental responses to increasing [CO₂]. Both non-targeted and targeted metabolite profiling by GC–MS and LC–MS were employed to examine primary and secondary metabolites in wheat (Triticum aestivum, cv Yocoro rojo) continuously exposed to these [CO₂] levels for 14, 21 and 28 days. Metabolite profile was altered by both [CO₂] and physiological age. In general, plants grown under high [CO₂] exhibited a metabolite profile characteristic of older plants under ambient CO₂. Elevated [CO₂] resulted in higher levels of phosphorylated sugar intermediates, though no clear trend in the content of reducing sugars was observed. Transient starch content was enhanced by increasing [CO₂] to a much greater extent at 10,000 ppm CO₂ than at 1500 ppm CO₂. The percentage increase of starch content resulting from CO₂ enrichment declined as plants develope. In contrast, elevated [CO₂] promoted the accumulation of secondary metabolites (flavonoids) progressively to a greater extent as plants became mature. Elevated [CO₂] to 1500 ppm induced a higher initial growth rate, while super-elevated [CO₂] appeared to negate such initial growth promotion. However, after 4 weeks, there was no difference in vegetative growth between 1500 and 10,000 ppm CO₂-grown plants, both elevated CO₂ levels resulted in an overall 25% increase in biomass over the control plants. More interestingly, elevated atmospheric [CO₂] reduced evapotranspiration rate (ET), but further increase to the supra-optimal level resulted in increased ET (a reversed trend), i.e. ET at 1500 ppm < ET at 10,000 ppm < ET at 400 ppm. The differential effect of elevated and super-elevated CO₂ on plants was further reflected in the nitrogen dynamics. These results provide the potential metabolic basis for the differential productivity and stomatal function of plants grown under elevated and super-elevated CO₂ levels.     

The Lunar Radar Sounder (LRS) Onboard the KAGUYA (SELENE) Spacecraft

The Lunar Radar Sounder (LRS) onboard the KAGUYA (SELENE) spacecraft has successfully performed radar sounder observations of the lunar subsurface structures and passive observations of natural radio and plasma waves from the lunar orbit. After the transfer of the spacecraft into the final lunar orbit and antenna deployment, the operation of LRS started on October 29, 2007. Through the operation until June 10, 2009, 2363 hours worth of radar sounder data and 8961 hours worth of natural radio and plasma wave data have been obtained. It was revealed through radar sounder observations that there are distinct reflectors at a depth of several hundred meters in the nearside maria, which are inferred to be buried regolith layers covered by a basalt layer with a thickness of several hundred meters. Radar sounder data were obtained not only in the nearside maria but also in other regions such as the farside highland region and polar region. LRS also performed passive observations of natural plasma waves associated with interaction processes between the solar wind plasma and the moon, and the natural waves from the Earth, the sun, and Jupiter. Natural radio waves such as auroral kilometric radiation (AKR) with interference patterns caused by the lunar surface reflections, and Jovian hectometric (HOM) emissions were detected. Intense electrostatic plasma waves around 20 kHz were almost always observed at local electron plasma frequency in the solar wind, and the electron density profile, including the lunar wake boundary, was derived along the spacecraft trajectory. Broadband noises below several kHz were frequently observed in the dayside and wake boundary of the moon and it was found that a portion of them consist of bipolar pulses. The datasets obtained by LRS will make contributions for studies on the lunar geology and physical processes of natural radio and plasma wave generation and propagation.     

Dark matter search with the CALET detector on-board ISS

The CALorimetric Electron Telescope, CALET, mission is proposed for the observation of high-energy electrons and gamma-rays at the Exposed Facility of the Japanese Experiment Module on the International Space Station. The CALET has a capability to observe the electrons (without separation between e⁺ and e⁻) in 1 GeV–10 TeV and the gamma-rays in 20 MeV–several TeV with a high-energy resolution of 2% at 100 GeV, a good angular resolution of 0.06 degree at 100 GeV, and a high proton-rejection power of nearly 10⁶. The CALET has a geometrical factor of 1 m²sr, and the observation period is expected for more than three years. The very precise measurement of electrons enables us to detect a distinctive feature in the energy spectrum caused from WIMP dark matter in the Galactic halo. The excellent energy resolution of CALET, which is much better than GLAST or air Cherenkov telescopes over 10 GeV, enables us to detect gamma-ray lines in the sub-TeV region from WIMP dark matter annihilations. The CALET has, therefore, a unique capability to search for WIMP dark matter by the hybrid observations of electrons and gamma-rays.     

A noise attenuation method for medium-energy electron measurements in the radiation belt

From the viewpoint of plasma particle measurements in the radiation belt, background noise is a serious problem. High-energy particles penetrating the sensor shielding generate spurious signals, and their count rate often can be comparable to the true signals. In order to attenuate such background noise during medium-energy (5–83 keV) electron measurements, we propose the double energy analyses (DEA) method. DEA is conducted by a combination of an electrostatic analyser (ESA) and avalanche photo-diodes (APDs); ESA and APD independently determine the energy of each incoming particle. By using the DEA method, therefore, the penetrating particles can be rejected when the two energy determinations are inconsistent; spurious noise are caused only when the deposited energy at an APD is by chance consistent with the measured energy by ESA. We formulate the noise count rate and show the advantage of DEA method quantitatively.     

Improvements in and actual performance of the Plant Experiment Unit onboard Kibo,the Japanese experiment module on the international space station

In 2004, Japan Aerospace Exploration Agency developed the engineered model of the Plant Experiment Unit and the Cell Biology Experiment Facility. The Plant Experiment Unit was designed to be installed in the Cell Biology Experiment Facility and to support the seed-to-seed life cycle experiment of Arabidopsis plants in space in the project named Space Seed. Ground-based experiments to test the Plant Experiment Unit showed that the unit needed further improvement of a system to control the water content of a seedbed using an infrared moisture analyzer and that it was difficult to keep the relative humidity inside the Plant Experiment Unit between 70 and 80% because the Cell Biology Experiment Facility had neither a ventilation system nor a dehumidifying system. Therefore, excess moisture inside the Cell Biology Experiment Facility was removed with desiccant bags containing calcium chloride. Eight flight models of the Plant Experiment Unit in which dry Arabidopsis seeds were fixed to the seedbed with gum arabic were launched to the International Space Station in the space shuttle STS-128 (17A) on August 28, 2009. Plant Experiment Unit were installed in the Cell Biology Experiment Facility with desiccant boxes, and then the Space Seed experiment was started in the Japanese Experiment Module, named Kibo, which was part of the International Space Station, on September 10, 2009 by watering the seedbed and terminated 2 months later on November 11, 2009. On April 19, 2010, the Arabidopsis plants harvested in Kibo were retrieved and brought back to Earth by the space shuttle mission STS-131 (19A). The present paper describes the Space Seed experiment with particular reference to the development of the Plant Experiment Unit and its actual performance in Kibo onboard the International Space Station. Downlinked images from Kibo showed that the seeds had started germinating 3 days after the initial watering. The plants continued growing, producing rosette leaves, inflorescence stems, flowers, and fruits in the Plant Experiment Unit. In addition, the senescence of rosette leaves was found to be delayed in microgravity.     

Cosmic-ray electron spectrum above 100 GeV from PPB-BETS experiment in Antarctica

Cosmic-ray electrons have been observed in the energy region from 10 GeV to 1 TeV with the PPB-BETS by a long duration balloon flight using a Polar Patrol Balloon (PPB) in Antarctica. The observation was carried out for 13 days at an average altitude of 35 km in January 2004. The PPB-BETS detector is an imaging calorimeter composed of scintillating-fiber belts and plastic scintillators inserted between lead plates. In the study of cosmic-ray electrons, there have been some suggestions that high-energy electrons above 100 GeV are a powerful probe to identify nearby cosmic-ray sources and search for particle dark matter. In this paper, we present the energy spectrum of cosmic-ray electrons in the energy range from 100 GeV to 1 TeV at the top of atmosphere, and compare our spectrum with the results from other experiments.     

JUXTA: A new probe of X-ray emission from the Jupiter system

For the future Japanese exploration mission of the Jupiter’s magnetosphere (JMO: Jupiter Magnetospheric Orbiter), a unique instrument named JUXTA (Jupiter X-ray Telescope Array) is being developed. It aims at the first in-situ measurement of X-ray emission associated with Jupiter and its neighborhood. Recent observations with Earth-orbiting satellites have revealed various X-ray emission from the Jupiter system. X-ray sources include Jupiter’s aurorae, disk emission, inner radiation belts, the Galilean satellites and the Io plasma torus. X-ray imaging spectroscopy can be a new probe to reveal rotationally driven activities, particle acceleration and Jupiter–satellite binary system. JUXTA is composed of an ultra-light weight X-ray telescope based on micromachining technology and a radiation-hard semiconductor pixel detector. It covers 0.3–2 keV with the energy resolution of <100 eV at 0.6 keV. Because of proximity to Jupiter (∼30 Jovian radii at periapsis), the image resolution of <5 arcmin and the on-axis effective area of >3 cm² at 0.6 keV allow extremely high photon statistics and high resolution observations.