Detection of microlesions induced by heavy ions using liposomes filled with fluorescent dye.

In cells irradiation by heavy ions has been hypothesized to produce microlesions, regions of local damage. In cell membranes this damage is thought to manifest itself in the form of holes. The primary evidence for microlesions comes from morphological studies of cell membranes, but this evidence is still controversial, especially since holes also have been observed in membranes of normal, nonirradiated, cells. However, it is possible that damage not associated with histologically discernable disruptions may still occur. In order to resolve this issue, we developed a system for detecting microlesions based on liposomes filled with fluorescent dye. We hypothesized that if microlesions form in these liposomes as the result of irradiation, then the entrapped dye will leak out into the surrounding medium in a measurable way. Polypropylene vials containing suspensions of vesicles composed of either dipalmitoyl phosphatidylcholine, or a combination of egg phosphatidylcholine and cholesterol were irradiated at the Brookhaven National Laboratory using 56Fe ions at 1 GeV/amu. In several cases we obtained a significant loss of the entrapped dye above the background level. Our results suggest that holes may form in liposomes as the result of heavy ion irradiation, and that these holes are large enough to allow leakage of cell internal contents that are at least as large as a 1 nm diameter calcein molecule.     

Raman imagery: a new approach to assess the geochemical maturity and biogenicity of permineralized precambrian fossils

Laser-Raman imagery is a non-intrusive, non-destructive analytical technique, recently introduced to Precambrian paleobiology, that can be used to demonstrate a one-to-one spatial correlation between the optically discernible morphology and kerogenous composition of permineralized fossil microorganisms. Made possible by the submicron-scale resolution of the technique and its high sensitivity to the Raman signal of carbonaceous matter, such analyses can be used to determine the chemical-structural characteristics of organic-walled microfossils and associated sapropelic carbonaceous matter in acid-resistant residues and petrographic thin sections. Here we use this technique to analyze kerogenous microscopic fossils and associated carbonaceous sapropel permineralized in 22 unmetamorphosed or little-metamorphosed fine-grained chert units ranging from approximately 400 to approximately 2,100 Ma old. The lineshapes of the Raman spectra acquired vary systematically with five indices of organic geochemical maturation: (1) the mineral-based metamorphic grade of the fossil-bearing units; (2) the fidelity of preservation of the fossils studied; (3) the color of the organic matter analyzed; and both the (4) H/C and (5) N/C ratios measured in particulate kerogens isolated from bulk samples of the fossil-bearing cherts. Deconvolution of relevant spectra shows that those of relatively well-preserved permineralized kerogens analyzed in situ exhibit a distinctive set of Raman bands that are identifiable also in hydrated organic-walled microfossils and particulate carbonaceous matter freed from the cherts by acid maceration. These distinctive Raman bands, however, become indeterminate upon dehydration of such specimens. To compare quantitatively the variations observed among the spectra measured, we introduce the Raman Index of Preservation, an approximate measure of the geochemical maturity of the kerogens studied that is consistent both with the five indices of organic geochemical alteration and with spectra acquired from fossils experimentally heated under controlled laboratory conditions. The results reported provide new insight into the chemical-structural characteristics of ancient carbonaceous matter, the physicochemical changes that accompany organic geochemical maturation, and a new criterion to be added to the suite of evidence by which to evaluate the origin of minute fossil-like objects of possible but uncertain biogenicity.     

The cost benefits of the Spacelab system to scientific investigations and space applications

In the past, one of the major problems in performing scientific investigations in space has been the high cost of developing, integrating, and transporting scientific experiments into space. The limited resources of unmanned spacecraft, coupled with the requirements for completely automated operations, was another factor contributing to the high costs of scientific research in space. In previous space missions after developing, integrating and transporting costly experiments into space and obtaining successful data, the experiment facility and spacecraft have been lost forever, because they could not be returned to earth. The objective of this paper is to present how the utilization of the Spacelab System will result in cost benefits to the scientific community, and significantly reduce the cost of space operations from previous space programs.The following approach was used to quantify the cost benefits of using the Spacelab System to greatly reduce the operational costs of scientific research in space. An analysis was made of the series of activities required to combine individual scientific experiments into an integrated payload that is compatible with the Space Transportation System (STS). These activities, including Shuttle and Spacelab integration, communications and data processing, launch support requirements, and flight operations were analyzed to indicate how this new space system, when compared with previous space systems, will reduce the cost of space research. It will be shown that utilization of the Spacelab modular design, standard payload interfaces, optional Mission Dependent Equipment (MDE), and standard services, such as the Experiment Computer Operating System (ECOS), allow the user many more services than previous programs, at significantly lower costs. In addition, the missions will also be analyzed to relate their cost benefit contributions to space scientific research.The analytical tools that are being developed at MSFC in the form of computer programs that can rapidly analyze experiment to Spacelab interfaces will be discussed to show how these tools allow the Spacelab integrator to economically establish the payload compatibility of a Spacelab mission.The information used in this paper has been assimilated from the actual experience gained in integrating over 50 highly complex, scientific experiments that will fly on the Spacelab first and second missions. In addition, this paper described the work being done at the Marshall Space Flight Center (MSFC) to define the analytical integration tools and techniques required to economically and efficiently integrate a wide variety of Spacelab payloads and missions. The conclusions reached in this study are based on the actual experience gained at MSFC in its roles of Spacelab integration and mission managers for the first three Spacelab missions. The results of this paper will clearly show that the cost benefits of the Spacelab system will greatly reduce the costs and increase the opportunities for scientific investigation from space.     

A Geosynchronous Orbit Search Strategy

Since more than 10 years there is evidence that small-size space debris is accumulating in the geosynchronous orbit (GEO), probably as the result of breakups. Two break-ups have been reported in GEO. The 1978 break-up of an EKRAN 2 satellite, SSN 10365, was identified in 1992, and in 1992 a Titan 3C Transtage, SSN 3432, break-up produced at least twenty observable pieces. Subsequently several nations performed optical surveys of the GEO region in the form of independent observation campaigns. Such surveys suffer from the fact that the field of view of optical telescopes is small compared with the total area covered by the GEO ring. As a consequence only a small volume of the orbital element-magnitude-space is covered by each individual survey. Results from these surveys are thus affected by observational biases and therefore difficult to compare. This paper describes the development of a common search strategy to overcome these limitations. The strategy optimizes the sampling for objects in orbits similar to the orbits of the known GEO population but does not exclude the detection of objects with other orbital planes. A properly designed common search strategy clearly eases the comparison of results from different groups and the extrapolation from the sparse (biased) samples to the entire GEO environment.     

Shuttle avionics verification and integration testing

The Space Shuttle Orbiter employs a fly-by-wire control system of 200 major avionic hardware devices interfacing with five flight computers through a complex data bus system. Responses to man-in-the-loop commands are dependent on the flight software. Early program development testing of the computer and avionic hardware has been accomplished at Rockwell International's Shuttle Avionics Development Laboratory (ADL). Hardware development has led to complete multi-string system testing and flight software evaluations. This paper provides an overview of the ADL. Its role and test capabilities in support of Shuttle development are defined. The nature of computer driven test programs for the Orbiter displays, the Digital Autopilot, and flight software development describe the test bed provided by the ADL.     

Radiative and hybrid cooling of infrared space telescopes

The designs of cold space telescopes, cryogenic and radiatively cooled, are similar in most elements and both benefit from orbits distant from the Earth. In particular such orbits allow the anti-sunward side of radiatively-cooled spacecraft to be used to provide large cooling radiators for the individual radiation shields. Designs incorporating these features have predictedT _tel near 20 K. The attainability of such temperatures is supported by limited practical experience (IRAS, COBE). Supplementary cooling systems (cryogens, mechanical coolers) can be advantageously combined with radiative cooling in hybrid designs to provide robustness against deterioration and yet lower temperatures for detectors, instruments, and even the whole telescope. The possibility of such major additional gains is illustrated by the Very Cold Telescope option under study forEdison, which should offerT _tel5 K for a little extra mechanical cooling capacity.     

Deep Impact: Working Properties for the Target Nucleus – Comet 9P/Tempel 1

In 1998, Comet 9P/Tempel 1 was chosen as the target of the Deep Impact mission (A’Hearn, M. F., Belton, M. J. S., and Delamere, A., Space Sci. Rev., 2005) even though very little was known about its physical properties. Efforts were immediately begun to improve this situation by the Deep Impact Science Team leading to the founding of a worldwide observing campaign (Meech et al., Space Sci. Rev., 2005a). This campaign has already produced a great deal of information on the global properties of the comet’s nucleus (summarized in Table I) that is vital to the planning and the assessment of the chances of success at the impact and encounter. Since the mission was begun the successful encounters of the Deep Space 1 spacecraft at Comet 19P/Borrelly and the Stardust spacecraft at Comet 81P/Wild 2 have occurred yielding new information on the state of the nuclei of these two comets. This information, together with earlier results on the nucleus of comet 1P/Halley from the European Space Agency’s Giotto, the Soviet Vega mission, and various ground-based observational and theoretical studies, is used as a basis for conjectures on the morphological, geological, mechanical, and compositional properties of the surface and subsurface that Deep Impact may find at 9P/Tempel 1. We adopt the following working values (circa December 2004) for the nucleus parameters of prime importance to Deep Impact as follows: mean effective radius = 3.25± 0.2 km, shape – irregular triaxial ellipsoid with a/b = 3.2± 0.4 and overall dimensions of ∼14.4 × 4.4 × 4.4 km, principal axis rotation with period = 41.85± 0.1 hr, pole directions (RA, Dec, J2000) = 46± 10, 73± 10 deg (Pole 1) or 287± 14, 16.5± 10 deg (Pole 2) (the two poles are photometrically, but not geometrically, equivalent), Kron-Cousins (V-R) color = 0.56± 0.02, V-band geometric albedo = 0.04± 0.01, R-band geometric albedo = 0.05± 0.01, R-band H(1,1,0) = 14.441± 0.067, and mass ∼7×10¹³ kg assuming a bulk density of 500 kg m⁻³. As these are working values, {i.e.}, based on preliminary analyses, it is expected that adjustments to their values may be made before encounter as improved estimates become available through further analysis of the large database being made available by the Deep Impact observing campaign. Given the parameters listed above the impact will occur in an environment where the local gravity is estimated at 0.027–0.04 cm s⁻² and the escape velocity between 1.4 and 2 m s⁻¹. For both of the rotation poles found here, the Deep Impact spacecraft on approach to encounter will find the rotation axis close to the plane of the sky (aspect angles 82.2 and 69.7 deg. for pole 1 and 2, respectively). However, until the rotation period estimate is substantially improved, it will remain uncertain whether the impactor will collide with the broadside or the ends of the nucleus.     

Cassini Imaging Science: Instrument Characteristics And Anticipated Scientific Investigations At Saturn

The Cassini Imaging Science Subsystem (ISS) is the highest-resolution two-dimensional imaging device on the Cassini Orbiter and has been designed for investigations of the bodies and phenomena found within the Saturnian planetary system. It consists of two framing cameras: a narrow angle, reflecting telescope with a 2-m focal length and a square field of view (FOV) 0.35^∘ across, and a wide-angle refractor with a 0.2-m focal length and a FOV 3.5^∘ across. At the heart of each camera is a charged coupled device (CCD) detector consisting of a 1024 square array of pixels, each 12 μ on a side. The data system allows many options for data collection, including choices for on-chip summing, rapid imaging and data compression. Each camera is outfitted with a large number of spectral filters which, taken together, span the electromagnetic spectrum from 200 to 1100 nm. These were chosen to address a multitude of Saturn-system scientific objectives: sounding the three-dimensional cloud structure and meteorology of the Saturn and Titan atmospheres, capturing lightning on both bodies, imaging the surfaces of Saturn’s many icy satellites, determining the structure of its enormous ring system, searching for previously undiscovered Saturnian moons (within and exterior to the rings), peering through the hazy Titan atmosphere to its yet-unexplored surface, and in general searching for temporal variability throughout the system on a variety of time scales. The ISS is also the optical navigation instrument for the Cassini mission. We describe here the capabilities and characteristics of the Cassini ISS, determined from both ground calibration data and in-flight data taken during cruise, and the Saturn-system investigations that will be conducted with it. At the time of writing, Cassini is approaching Saturn and the images returned to Earth thus far are both breathtaking and promising.This revised version was published online in July 2005 with a corrected cover date.     

Comet Geology with Deep Impact Remote Sensing

The Deep Impact mission will provide the highest resolution images yet of a comet nucleus. Our knowledge of the makeup and structure of cometary nuclei, and the processes shaping their surfaces, is extremely limited, thus use of the Deep Impact data to show the geological context of the cratering experiment is crucial. This article briefly discusses some of the geological issues of cometary nuclei.     

Acceleration of the solar wind

In this review, we discuss critically recent research on the acceleration of the solar wind, giving emphasis to high-speed solar wind streams emanating from solar coronal holes. We first explain why thermally driven wind models constrained by solar and interplanetary observations encounter substantial difficulties in explaining high speed streams. Then, through a general discussion of energy addition to the solar wind above the coronal base, we indicate a possible resolution of these difficulties. Finally, we consider the question of what role MHD waves might play in transporting energy through the solar atmosphere and depositing it in the solar wind, and we conclude by examining, in a simple way, the specific mechanism of solar wind acceleration by Alfvén waves and the related problem of accelerating massive stellar winds with Alfvén waves.Paper presented at the IX-th Lindau Workshop The Source Region of the Solar Wind.On leave from the Auroral Observatory, Institute of Mathematical and Physical Sciences, University of Tromsø, N-9001 Tromsø, Norway.The National Center for Atmospheric Research is sponsored by the National Science Foundation.