Gravity effects on connective tissue biosynthesis by cultured mesenchymal cells.

Quantitative and qualitative aspects of collagen synthesis under microgravity, normal gravity and hypergravity conditions were investigated during the spacelab D-2 mission by incubating human fibroblast cultures with [3H]-proline for 0, 4, 7, 10 and 20 hours. Quantitative analysis revealed an increase of collagen synthesis under microgravity conditions, being 40% higher than 1g controls. Hypergravity samples at 1.44g, 6.6g and 10g showed a decrease in collagen synthesis with increasing g, being down to about 15% at 10g. The relative proportion of collagen from total protein synthesized, the secretion of collagen by the cells, proline hydroxylation of individual collagen alpha-chains and the relative proportions of collagens I, III and V synthesized were not affected at any of the applied conditions.     

RPC-MAG The Fluxgate Magnetometer in the ROSETTA Plasma Consortium

The fluxgate magnetometer experiment onboard the ROSETTA spacecraft aims to measure the magnetic field in the interaction region of the solar wind plasma with comet 67P/Churyumov-Gerasimenko. It consists of a system of two ultra light (about 28 g each ) triaxial fluxgate magnetometer sensors, mounted on the 1.5 m long spacecraft boom. The measurement range of each sensor is ±16384 nT with quantization steps of 31 pT. The magnetometer sensors are operated with a time resolution of up to 0.05 s, corresponding to a bandwidth of 0–10 Hz. This performance of the RPC-MAG sensors allows detailed analyses of magnetic field variations in the cometary environment. RPC-MAG furthermore is designed to study possible remnant magnetic fields of the nucleus, measurements which will be done in close cooperation with the ROSETTA lander magnetometer experiment ROMAP.     

Spectroscopic Measurement of Coronal Compositions

Although the elemental composition in all parts of the solar photosphere appears to be the same this is clearly not the case with the solar upper atmosphere (SUA). Spectroscopic studies show that in the corona elemental composition along solar equatorial regions is usually different from polar regions; composition in quiet Sun regions is often different from coronal hole and active region compositions and the transition region composition is frequently different from the coronal composition along the same line of sight. In the following two issues are discussed. The first involves abundance ratios between the high-FIP O and Ne and the low-FIP Mg and Fe that are important for meaningful comparisons between photospheric and SUA compositions and the second involves a review of composition and time variability of SUA plasmas at heights of 1.0≤h≤1.5R _⊙.     

The Genesis Solar Wind Concentrator Target: Mass Fractionation Characterised by Neon Isotopes

The concentrator on Genesis provided samples of increased fluences of solar wind ions for precise determination of the oxygen isotopic composition. The concentration process caused mass fractionation as a function of the radial target position. This fractionation was measured using Ne released by UV laser ablation and compared with modelled Ne data, obtained from ion-trajectory simulations. Measured data show that the concentrator performed as expected and indicate a radially symmetric concentration process. Measured concentration factors are up to ∼30 at the target centre. The total range of isotopic fractionation along the target radius is 3.8%/amu, with monotonically decreasing ²⁰Ne/²²Ne towards the centre, which differs from model predictions. We discuss potential reasons and propose future attempts to overcome these disagreements.     

Space Shuttle UHF communications performance evaluation

Computational investigations and experimental measurements were performed to evaluate the Space Shuttle UHF communication system performance for payload bay antenna at the proposed new location. To insure adequate communication coverage at relocated new location, the link margin for the Extravehicular Activity (EVA) astronauts and between Space Shuttle Orbiter and International Space Station (SSO-ISS) during rendezvous were analyzed. The multipath effects from the vehicle structures surrounding the antenna were investigated. The Radio Frequency (RF) electromagnetic radiation to the Orbiter Docking System (ODS) pyrotechnics was also analyzed to ensure the EMC/EMI compliances.     

Road profile recognition for autonomous car navigation and Navstar GPS support

We discuss autonomous car navigation based on updating dead reckoning (DR) by road profile recognition (RPR). The navigation system requires sensors to detect changes in altitude and driving direction which are installed in modern cars for different purposes (e.g. ABS sensors). The layout of the navigation system is discussed and simulations are carried out over driving distances of approximately 150 km on the basis of realistic road data and ordinary sensor accuracies. Positioning errors of lower than 10 m (standard deviation) are observed. To achieve this accuracy the synchronization error between measured and mapped data must be continually estimated. The introduced navigation method is ideal to complete present commercial car navigation systems using Navstar GPS.     

Surface characteristics of spacecraft components affect the aggregation of microorganisms and may lead to different survival rates of bacteria on Mars landers

Layers of dormant endospores of Bacillus subtilis HA101 were applied to eight different spacecraft materials and exposed to martian conditions of low pressure (8.5 mbar), low temperature (-10 degrees C), and high CO(2) gas composition and irradiated with a Mars-normal ultraviolet (UV-visible- near-infrared spectrum. Bacterial layers were exposed to either 1 min or 1 h of Mars-normal UV irradiation, which simulated clear-sky conditions on equatorial Mars (0.1 tau). When exposed to 1 min of Mars UV irradiation, the numbers of viable endospores of B. subtilis were reduced three to four orders of magnitude for two brands of aluminum (Al), stainless steel, chemfilm-treated Al, clear-anodized Al, and black-anodized Al coupons. In contrast, bacterial survival was reduced only one to two orders of magnitude for endospores on the non-metal materials astroquartz and graphite composite when bacterial endospores were exposed to 1 min of Mars UV irradiation. When bacterial monolayers were exposed to 1 h of Mars UV irradiation, no viable bacteria were recovered from the six metal coupons listed above. In contrast, bacterial survival was reduced only two to three orders of magnitude for spore layers on astroquartz and graphite composite exposed to 1 h of Mars UV irradiation. Scanning electron microscopy images of the bacterial monolayers on all eight spacecraft materials revealed that endospores of B. subtilis formed large aggregates of multilayered spores on astroquartz and graphite composite, but not on the other six spacecraft materials. It is likely that the formation of multilayered aggregates of endospores on astroquartz and graphite composite is responsible for the enhanced survival of bacterial cells on these materials.     

Numerical Simulation of Impact Loading on Solid Propellant Within an Orthotropic Shell

The strain–stress state of the solid propellant rocket engines (SPREs) is simulated under impact. The effect of orientation of elastic and strength properties of orthotropic organoplastic shell material on the strain–stress state of the solid propellant is investigated. Normal and oblique impact of single steel cylinder projectiles, both simultaneous and at different times of multiple, converging steel spheric particles with SPRE are investigated in this study. The investigation is conducted numerically. The numerical modeling was carried out in a three-dimensional formulation by the method of finite elements for the continuous approach of the mechanics of a deformable solid. The destruction of the anisotropic material is described by the tensor-polynomial criterion of the fourth degree, which takes into account the influence of hydrostatic pressure.     

Raman imagery: a new approach to assess the geochemical maturity and biogenicity of permineralized precambrian fossils

Laser-Raman imagery is a non-intrusive, non-destructive analytical technique, recently introduced to Precambrian paleobiology, that can be used to demonstrate a one-to-one spatial correlation between the optically discernible morphology and kerogenous composition of permineralized fossil microorganisms. Made possible by the submicron-scale resolution of the technique and its high sensitivity to the Raman signal of carbonaceous matter, such analyses can be used to determine the chemical-structural characteristics of organic-walled microfossils and associated sapropelic carbonaceous matter in acid-resistant residues and petrographic thin sections. Here we use this technique to analyze kerogenous microscopic fossils and associated carbonaceous sapropel permineralized in 22 unmetamorphosed or little-metamorphosed fine-grained chert units ranging from approximately 400 to approximately 2,100 Ma old. The lineshapes of the Raman spectra acquired vary systematically with five indices of organic geochemical maturation: (1) the mineral-based metamorphic grade of the fossil-bearing units; (2) the fidelity of preservation of the fossils studied; (3) the color of the organic matter analyzed; and both the (4) H/C and (5) N/C ratios measured in particulate kerogens isolated from bulk samples of the fossil-bearing cherts. Deconvolution of relevant spectra shows that those of relatively well-preserved permineralized kerogens analyzed in situ exhibit a distinctive set of Raman bands that are identifiable also in hydrated organic-walled microfossils and particulate carbonaceous matter freed from the cherts by acid maceration. These distinctive Raman bands, however, become indeterminate upon dehydration of such specimens. To compare quantitatively the variations observed among the spectra measured, we introduce the Raman Index of Preservation, an approximate measure of the geochemical maturity of the kerogens studied that is consistent both with the five indices of organic geochemical alteration and with spectra acquired from fossils experimentally heated under controlled laboratory conditions. The results reported provide new insight into the chemical-structural characteristics of ancient carbonaceous matter, the physicochemical changes that accompany organic geochemical maturation, and a new criterion to be added to the suite of evidence by which to evaluate the origin of minute fossil-like objects of possible but uncertain biogenicity.