Bacterial community in ancient Siberian permafrost as characterized by culture and culture-independent methods

The microbial composition of ancient permafrost sediments from the Kolyma lowland of Northeast Eurasia was examined through culture and culture-independent approaches. These sediments have been continuously frozen for 5,000 to 2-3 million years. A total of 265 Bacteria 16S rRNA gene sequences were amplified from the permafrost total-community genomic DNA and screened by amplified ribosomal 16S rRNA restriction analysis. Members of three major lineages were found: gamma-Proteobacteria (mostly Xanthomonadaceae), Actinobacteria, and Firmicutes. We also determined partial 16S rRNA gene sequences of 49 isolates from a collection of 462 aerobes isolated from these sediments. The bacteria included Actinomycetales (Arthrobacter and Microbacteriaceae); followed by the Firmicutes (Exiguobacterium and Planomicrobium); the Bacteroidetes (Flavobacterium); the gamma-Proteobacteria (Psychrobacter); and the alpha-Proteobacteria (Sphingomonas). Both culture and culture-independent approaches showed the presence of high and low G+C Gram-positive bacteria and gamma-Proteobacteria. Some of the 16S rRNA gene sequences of environmental clones matched those of Arthrobacter isolates. Two-thirds of the isolates grew at -2.5 degrees C, indicating that they are psychroactive, and all are closely related to phylogenetic groups with strains from other cold environments, mostly commonly from Antarctica. The culturable and non-culturable microorganisms found in the terrestrial permafrost provide a prototype for possible life on the cryogenic planets of the Solar System.     

Some qualitative manifestations of the physical libration of the Moon by observing stars from the lunar surface

Targets and problems of the future Japanese project ILOM (In situ Lunar Orientation Measurement), which is planned to be realized as one kind of observations of lunar rotation at the second stage of SELENE-2 mission, are briefly described in the article. Inverse problem of lunar physical libration is formulated and solved. Accuracy of libration angles depending on accuracy of measuring selenographic coordinates is estimated. It is shown that selenographic coordinates of polar stars are insensitive to longitudinal librations τ(t). Comparing coordinates calculated for two models of a rigid and deformable Moon is carried out and components sensitive to Love number k₂ and to anelastic time delay are revealed.     

Viability of barley seeds after long-term exposure to outer side of international space station

Barley seeds were exposed to outer space for 13 months in a vented metal container without a climate control system to assess the risk of physiological and genetic mutation during long-term storage in space. The space-stored seeds (S0 generation), with an 82% germination rate in 50 seeds, lost about 20% of their weight after the exposure. The germinated seeds showed normal growth, heading, and ripening. The harvested seeds (S1 generation) also germinated and reproduced (S2 generation) as did the ground-stored seeds. The culm length, ear length, number of seed, grain weight, and fertility of the plants from the space-stored seeds were not significantly different from those of the ground-stored seeds in each of the S0 and S1 generation. Furthermore, the S1 and S2 space-stored seeds respectively showed similar β-glucan content to those of the ground-stored seeds. Amplified fragment length polymorphism analysis with 16 primer combinations showed no specific fragment that appears or disappears significantly in the DNA isolated from the barley grown from the space-stored seeds. Though these data are derived from nine S0 space-stored seeds in a single exposure experiment, the results demonstrate the preservation of barley seeds in outer space for 13 months without phenotypic or genotypic changes and with healthy and vigorous growth in space.     

Effects of the geomagnetic storm on October 29–31, 2003, on mid-latitude short-wave radio paths (the data of Doppler measurements)

We present the results of spectral analysis of a signal of oblique sounding of the ionosphere. This signal was received at the Doppler phase-angle measuring facility of Kazan University during the geomagnetic storm on October 29–31, 2003. On various short-wave (SW) radio paths, intense variations of Doppler frequency shift were observed. These variations exceeded variations observed on days without geomagnetic disturbances by an order of magnitude (and by two orders of magnitude during some short periods). Relatively short periods (1–3.5 min) are predominant on these days in variations of the Doppler frequency shift. It should be noted that, while for weak geomagnetic disturbances the response in the form of distortions of SW signal parameters was observed, as a rule, only for the paths coming from high-latitude regions, in the given case the effect was observed for all radio paths.Translated from Kosmicheskie Issledovaniya, Vol. 42, No. 6, 2004, pp. 640–644.Original Russian Text Copyright © 2004 by Bochkarev, Petrova, Teplov.     

Analysis of the Informativity of the Earth’s Magnetic Field in Near-Earth Space

Cosmic Research - This paper presents the results of a study of the informativity of the anomalies of the modulus and components of the Earth’s magnetic field in near-Earth space in the...     

Application of HF Doppler measurements for the investigation of internal atmospheric waves in the ionosphere

We present results of the spectral analysis of data series of Doppler frequency shifted signals reflected from the ionosphere, using experimental data received at Kazan University, Russia. Spectra of variations with periods from 1 min to 60 days have been calculated and analyzed for different scales of periods. The power spectral density for spring and winter differs by a factor of 3–4. Local maxima of variation amplitude are detected, which are statistically significant. The periods of these amplitude increases range from 6 to 12 min for winter, and from 24 to 48 min for autumn. Properties of spectra for variations with the periods of 1–72 h have been analyzed. The maximum of variation intensity for all seasons and frequencies corresponds to the period of 24 h. Spectra of variations with periods from 3 to 60 days have been calculated. The maxima periods of power spectral density have been detected by the MUSIC method for the high spectral resolution. The detected periods correspond to planetary wave periods. Analysis of spectra for days with different level of geomagnetic activity shows that the intensity of variations for days with a high level of geomagnetic activity is higher.     

Earth-Based Visible and Near-IR Imaging of Mercury

New planned orbiter missions to Mercury have prompted renewed efforts to investigate the surface of Mercury via ground-based remote sensing. While the highest resolution instrumentation optical telescopes (e.g., HST) cannot be used at angular distances close to the Sun, advanced ground-based astronomical techniques and modern analytical and software can be used to obtain the resolved images of the poorly known or unknown part of Mercury. Our observations of the planet presented here were carried out in many observatories at morning and evening elongation of the planet. Stacking the acquired images of the hemisphere of Mercury, which was not observed by the Mariner 10 mission (1974–1975), is presented. Huge features found there change radically the existing hypothesis that the “continental” character of a surface may be attributed to the whole planet. We present the observational method, the data analysis approach, the resulting images and obtained properties of the Mercury’s surface.     

Exobiological investigations on Russian spacecrafts

To investigate the possibility of prebiotic synthesis of organic molecules in open space, conditions involved irradiating nucleosides and inorganic phosphate during five Earth-orbiting Russian space missions that included Salut-7 (13- and 16-month missions), Mir, Bion-11, and Cosmos-2044. Dry films of samples were exposed from 2 weeks up to 16 months to the entire set of factors encountered in open space during Earth-orbiting missions. After each mission, products synthesized during flight and any compounds that remained undegraded were analyzed. The analyses demonstrated that increased flight duration led to the decay of both synthesized nucleotides and initial nucleosides. Corresponding laboratory experiments indicated that infrared radiation caused the greatest amount of decay to products of prebiotic reactions. Experiments revealed that 5'-mononucleotides were the main chemical products of the major derivatives synthesized of certain nucleosides. Exposure to ultraviolet (UV) C(145) was more effective than UVC(254) in producing a comparatively higher yield of mononucleotides, while the energy flux of the latter was one order of magnitude less (10(-7) as compared with 10(-6) for UVC(145)). In the course of the laboratory simulation experiments the heating of solid samples yielded the greatest production amount (6.34% for adenosine derivatives).     

Prospects in the orbital and rotational dynamics of the Moon with the advent of sub-centimeter lunar laser ranging

Lunar laser ranging (LLR) measurements are crucial for advanced exploration of the laws of fundamental gravitational physics and geophysics as well as for future human and robotic missions to the Moon. The corner-cube reflectors (CCR) currently on the Moon require no power and still work perfectly since their installation during the project Apollo era. Current LLR technology allows us to measure distances to the Moon with a precision approaching 1 mm. As NASA pursues the vision of taking humans back to the Moon, new, more precise laser ranging applications will be demanded, including continuous tracking from more sites on Earth, placing new CCR arrays on the Moon, and possibly installing other devices such as transponders, etc. for multiple scientific and technical purposes. Since this effort involves humans in space, then in all situations the accuracy, fidelity, and robustness of the measurements, their adequate interpretation, and any products based on them, are of utmost importance. Successful achievement of this goal strongly demands further significant improvement of the theoretical model of the orbital and rotational dynamics of the Earth–Moon system. This model should inevitably be based on the theory of general relativity, fully incorporate the relevant geophysical processes, lunar librations, tides, and should rely upon the most recent standards and recommendations of the IAU for data analysis. This paper discusses methods and problems in developing such a mathematical model. The model will take into account all the classical and relativistic effects in the orbital and rotational motion of the Moon and Earth at the sub-centimeter level. The model is supposed to be implemented as a part of the computer code underlying NASA Goddard’s orbital analysis and geophysical parameter estimation package GEODYN and the ephemeris package PMOE 2003 of the Purple Mountain Observatory. The new model will allow us to navigate a spacecraft precisely to a location on the Moon. It will also greatly improve our understanding of the structure of the lunar interior and the nature of the physical interaction at the core–mantle interface layer. The new theory and upcoming millimeter LLR will give us the means to perform one of the most precise fundamental tests of general relativity in the solar system.     

Use of global ionospheric maps for HF Doppler measurements interpretation

The HF Doppler technique, a method of measurement of Doppler frequency shift of ionospheric signal, is one of the well-known and widely used techniques of ionosphere research. It allows investigation of various disturbances in the ionosphere. There are different sources of disturbances in the ionosphere such as geomagnetic storms, solar flashes, meteorological effects and atmospheric waves. The HF Doppler technique allows us to find out the influence of earthquakes, explosions and other processes on the ionosphere, which occurs near the Earth. HF Doppler technique has high sensitivity to small frequency variations and high time resolution but interpretation of results is difficult. In this paper, we attempt to use GPS data for Doppler measurements interpretation. Modeling of Doppler frequency shift variations with use of TEC allows separation of ionosphere disturbances of medium scale.