Detection of microlesions induced by heavy ions using liposomes filled with fluorescent dye.

In cells irradiation by heavy ions has been hypothesized to produce microlesions, regions of local damage. In cell membranes this damage is thought to manifest itself in the form of holes. The primary evidence for microlesions comes from morphological studies of cell membranes, but this evidence is still controversial, especially since holes also have been observed in membranes of normal, nonirradiated, cells. However, it is possible that damage not associated with histologically discernable disruptions may still occur. In order to resolve this issue, we developed a system for detecting microlesions based on liposomes filled with fluorescent dye. We hypothesized that if microlesions form in these liposomes as the result of irradiation, then the entrapped dye will leak out into the surrounding medium in a measurable way. Polypropylene vials containing suspensions of vesicles composed of either dipalmitoyl phosphatidylcholine, or a combination of egg phosphatidylcholine and cholesterol were irradiated at the Brookhaven National Laboratory using 56Fe ions at 1 GeV/amu. In several cases we obtained a significant loss of the entrapped dye above the background level. Our results suggest that holes may form in liposomes as the result of heavy ion irradiation, and that these holes are large enough to allow leakage of cell internal contents that are at least as large as a 1 nm diameter calcein molecule.     

Towards a coherent remote sensing data policy

Access to space-based remote sensing data is critical for Earth science and the study of global change. This article summarizes a variety of US government Earth science data policies and problems. The authors examine current efforts to develop data policies for the next generation of US remote sensing programmes, noting likely problems based on past experiences. They argue that the goal of US Earth science data policy should be to provide the widest possible dissemination of data. Setting such a goal permits the development of a simple, coherent data policy that serves scientific, commercial and US government interests.     

A long-term space policy for Europe

This is an abridged and edited version of the second report of ESA's Long-Term Space Policy Committee, published in May 1999. Emphasizing the importance of space-based technologies to so many earthly activities, it argues that only greater European involvement in the field will ensure that the continent remains an autonomous and significant player in world affairs and illustrates this by proposing space solutions to three major challenges for the future. The report's action plan for making use of these solutions is summarized and the consequences of failing to act are spelled out using the historical example of China.     

Modal control of the planar motion of a long flexible beam in orbit

Attitude control techniques for the pointing and stabilization of very large, inherently flexible spacecraft systems are investigated. The attitude dynamics and control of a long, homogeneous flexible beam whose center of mass is assumed to follow a circular orbit is analyzed. In this study, first order effects of gravity-gradient are included, whereas external perturbations and related orbital station keeping maneuvers are neglected. A mathematical model which describes the system deflections within the orbital plane has been developed by treating the beam as having a maximum of three discretized mass particles connected by massless, elastic structural elements. The uncontrolled dynamics of this system are simulated and, in addition, the effects of the control devices are considered. The concept of distributed modal control, which provides a means for controlling a system mode independently of all other modes, is examined. The effect of varying the number of modes in the model as well as the number and location of the control devices are also considered.     

Raman imagery: a new approach to assess the geochemical maturity and biogenicity of permineralized precambrian fossils

Laser-Raman imagery is a non-intrusive, non-destructive analytical technique, recently introduced to Precambrian paleobiology, that can be used to demonstrate a one-to-one spatial correlation between the optically discernible morphology and kerogenous composition of permineralized fossil microorganisms. Made possible by the submicron-scale resolution of the technique and its high sensitivity to the Raman signal of carbonaceous matter, such analyses can be used to determine the chemical-structural characteristics of organic-walled microfossils and associated sapropelic carbonaceous matter in acid-resistant residues and petrographic thin sections. Here we use this technique to analyze kerogenous microscopic fossils and associated carbonaceous sapropel permineralized in 22 unmetamorphosed or little-metamorphosed fine-grained chert units ranging from approximately 400 to approximately 2,100 Ma old. The lineshapes of the Raman spectra acquired vary systematically with five indices of organic geochemical maturation: (1) the mineral-based metamorphic grade of the fossil-bearing units; (2) the fidelity of preservation of the fossils studied; (3) the color of the organic matter analyzed; and both the (4) H/C and (5) N/C ratios measured in particulate kerogens isolated from bulk samples of the fossil-bearing cherts. Deconvolution of relevant spectra shows that those of relatively well-preserved permineralized kerogens analyzed in situ exhibit a distinctive set of Raman bands that are identifiable also in hydrated organic-walled microfossils and particulate carbonaceous matter freed from the cherts by acid maceration. These distinctive Raman bands, however, become indeterminate upon dehydration of such specimens. To compare quantitatively the variations observed among the spectra measured, we introduce the Raman Index of Preservation, an approximate measure of the geochemical maturity of the kerogens studied that is consistent both with the five indices of organic geochemical alteration and with spectra acquired from fossils experimentally heated under controlled laboratory conditions. The results reported provide new insight into the chemical-structural characteristics of ancient carbonaceous matter, the physicochemical changes that accompany organic geochemical maturation, and a new criterion to be added to the suite of evidence by which to evaluate the origin of minute fossil-like objects of possible but uncertain biogenicity.     

The cost benefits of the Spacelab system to scientific investigations and space applications

In the past, one of the major problems in performing scientific investigations in space has been the high cost of developing, integrating, and transporting scientific experiments into space. The limited resources of unmanned spacecraft, coupled with the requirements for completely automated operations, was another factor contributing to the high costs of scientific research in space. In previous space missions after developing, integrating and transporting costly experiments into space and obtaining successful data, the experiment facility and spacecraft have been lost forever, because they could not be returned to earth. The objective of this paper is to present how the utilization of the Spacelab System will result in cost benefits to the scientific community, and significantly reduce the cost of space operations from previous space programs.The following approach was used to quantify the cost benefits of using the Spacelab System to greatly reduce the operational costs of scientific research in space. An analysis was made of the series of activities required to combine individual scientific experiments into an integrated payload that is compatible with the Space Transportation System (STS). These activities, including Shuttle and Spacelab integration, communications and data processing, launch support requirements, and flight operations were analyzed to indicate how this new space system, when compared with previous space systems, will reduce the cost of space research. It will be shown that utilization of the Spacelab modular design, standard payload interfaces, optional Mission Dependent Equipment (MDE), and standard services, such as the Experiment Computer Operating System (ECOS), allow the user many more services than previous programs, at significantly lower costs. In addition, the missions will also be analyzed to relate their cost benefit contributions to space scientific research.The analytical tools that are being developed at MSFC in the form of computer programs that can rapidly analyze experiment to Spacelab interfaces will be discussed to show how these tools allow the Spacelab integrator to economically establish the payload compatibility of a Spacelab mission.The information used in this paper has been assimilated from the actual experience gained in integrating over 50 highly complex, scientific experiments that will fly on the Spacelab first and second missions. In addition, this paper described the work being done at the Marshall Space Flight Center (MSFC) to define the analytical integration tools and techniques required to economically and efficiently integrate a wide variety of Spacelab payloads and missions. The conclusions reached in this study are based on the actual experience gained at MSFC in its roles of Spacelab integration and mission managers for the first three Spacelab missions. The results of this paper will clearly show that the cost benefits of the Spacelab system will greatly reduce the costs and increase the opportunities for scientific investigation from space.     

A Geosynchronous Orbit Search Strategy

Since more than 10 years there is evidence that small-size space debris is accumulating in the geosynchronous orbit (GEO), probably as the result of breakups. Two break-ups have been reported in GEO. The 1978 break-up of an EKRAN 2 satellite, SSN 10365, was identified in 1992, and in 1992 a Titan 3C Transtage, SSN 3432, break-up produced at least twenty observable pieces. Subsequently several nations performed optical surveys of the GEO region in the form of independent observation campaigns. Such surveys suffer from the fact that the field of view of optical telescopes is small compared with the total area covered by the GEO ring. As a consequence only a small volume of the orbital element-magnitude-space is covered by each individual survey. Results from these surveys are thus affected by observational biases and therefore difficult to compare. This paper describes the development of a common search strategy to overcome these limitations. The strategy optimizes the sampling for objects in orbits similar to the orbits of the known GEO population but does not exclude the detection of objects with other orbital planes. A properly designed common search strategy clearly eases the comparison of results from different groups and the extrapolation from the sparse (biased) samples to the entire GEO environment.     

Operational continuity of the global meteorological satellite network

The global network of meteorological satellites used different forms of international cooperation during its development, and some of these forms continue. Concern about continued operation of the global network led the WMO to study the issues and to adopt a long-term policy and strategy based on a shared meteorological satellite network. Nations need to consider how to combine their meteorological and space-related organizations in a partnership role, so they can directly contribute to a future global network of meteorological satellites. Some examples are cited to demonstrate that increased direct participation is a valid and feasible objective.     

Control of an orbiting flexible square platform in the presence of solar radiation

A mathematical model for the solar radiation forces and moments acting on a square plate (platform) in orbit is obtained by considering the plate mode shapes as combinations of free-free beam shape functions. The moment expressions for a plate of arbitrary reflectivity coefficient are obtained as a function of the solar incidence angle. It is seen that only the first three flexible modes of the plate generate a first order net moment about the center of mass, and that the solar radiation pressure does not influence the flexible modes of the plate for small amplitude vibrations. The solar radiation disturbance model is then included in the dynamic model of a square plate nominally oriented along the local vertical and having the major surface of the plate normal to the orbital plane. The roll angle of the plate is seen to increase steadily due to the solar radiation pressure whereas the pitch and yaw motions oscillate with an amplitude of approximately 0.2° for a 100 m square thin aluminum plate in synchronous orbit. To control the shape and orientation of the plate two point actuators are assumed—one whose force axis is normal to the plane of the plate, the second with a force axis in the plane of the plate. The control law and the feedback gain values are obtained based on linear quadratic Gaussian methods. Transient responses and control requirements are simulated for local vertical and horizontal orientations.     

Shuttle avionics verification and integration testing

The Space Shuttle Orbiter employs a fly-by-wire control system of 200 major avionic hardware devices interfacing with five flight computers through a complex data bus system. Responses to man-in-the-loop commands are dependent on the flight software. Early program development testing of the computer and avionic hardware has been accomplished at Rockwell International's Shuttle Avionics Development Laboratory (ADL). Hardware development has led to complete multi-string system testing and flight software evaluations. This paper provides an overview of the ADL. Its role and test capabilities in support of Shuttle development are defined. The nature of computer driven test programs for the Orbiter displays, the Digital Autopilot, and flight software development describe the test bed provided by the ADL.