Focal contacts organization in osteoblastic cells under microgravity and cyclic deformation conditions.

We compared quantitatively vinculin-related adhesion parameters in osteoblastic cells submitted to opposite mechanical stresses, i.e., low deformation and frequency strain regimens (stretch condition) and microgravity exposure (relaxed condition). Cyclic deformation induced a biphasic response comprising new focal contacts formation followed by their clustering in ROS cells. Microgravity exposure induced a reduction in focal contact number and clustering in ROS cells. We previously demonstrated that 1% cyclic deformations at 0.05 Hz during a daily 10 min episode over 7 days stimulated ROS 17/2.8 growth as compared to static culture whereas relaxed ROS proliferated similarly to static culture (BC). To evaluate whether the proliferation (stretch) or the survival (relaxed) status of ROS cells influences focal contact organization, we inhibited ERKs proliferative-dependent pathway. Inhibition of proliferation by PD98059 was overcome although not fully restored by stretch. Furthermore stretch-induced clustering of vinculin-positive contacts still occurs in the presence of ERKs inhibitor, whereas the increase in focal contact number is abolished. In conclusion, we showed that focal contacts are mechanoeffectors and that hyper-mechanical stimulation could up regulate focal contacts size as compared to hypo-mechanical that down regulate clusterization.     

The Urey instrument: an advanced in situ organic and oxidant detector for Mars exploration

The Urey organic and oxidant detector consists of a suite of instruments designed to search for several classes of organic molecules in the martian regolith and ascertain whether these compounds were produced by biotic or abiotic processes using chirality measurements. These experiments will also determine the chemical stability of organic molecules within the host regolith based on the presence and chemical reactivity of surface and atmospheric oxidants. Urey has been selected for the Pasteur payload on the European Space Agency's (ESA's) upcoming 2013 ExoMars rover mission. The diverse and effective capabilities of Urey make it an integral part of the payload and will help to achieve a large portion of the mission's primary scientific objective: "to search for signs of past and present life on Mars." This instrument is named in honor of Harold Urey for his seminal contributions to the fields of cosmochemistry and the origin of life.     

Response of the magnetosphere during early August 1972

The solar wind velocity and interplanetary magnetic field were unusually high late on 4 August and early on 5 August, 1972. The magnetopause was close to or below 6.6 R _e from 2117 to 2318 UT and close to or below 5.1 R _e from 2236 to 2318 UT on 4 August. The magnetosheath field near noon was several hundred gammas and frequently south during these intervals, and there was some evidence of field erosion. The entry of solar wind plasma into the inner magnetosphere during this period was not unusually high, however. Proton energy density was lower than in the storms of December 1971, and June 1972. The plasmapause steadily moved inward on 4 and 5 August; it reached 2 R _e before expanding on 6 August. The unusually high amplitude magnetic pulsations commenced near 2240 UT, 4 August, and lasted until near noon on 5 August. Both the close magnetopause and the large pulsations appear to be due to the high solar wind velocity following the shock that reached Earth at 2054 UT on 4 August.     

Magnetic field measurements in space

Conclusions The magnetosphere boundary has been penetrated in several places, conflicting evidence about the ring current location has been found, and the field exterior to the boundary has revealed some unexpected features. Pronouncements about the structure of the geomagnetic and interplanetary magnetic fields are still based on scanty evidence but the experimental basis of such estimates is more adequate than in 1958.The boundary between the geomagnetic field and the interplanetary medium has been found, by Explorer XII, to be located at approximately 10 R _E on the sunlit side of the earth near the equator. It has been observed to fluctuate between 8 and 12 R _E during August, September and October of 1961. During several days in March, 1961, the boundary, on the dark side of the earth, was penetrated repeatedly by Explorer X on an outbound pass near 135° from the earth-sun line. Several interpretations are possible; the most reasonable one at present is that the boundary was fluctuating in this period, placing the satellite alternately inside the geomagnetic field and outside in a region of turbulent magnetic fields and plasma flow.A region of turbulent magnetic fields was also observed by Pioneer I, Pioneer V, and Explorer XII between 10 and 15 R _E on the sunlit side of the earth. Pioneer V observed also a steady field 2 to 5 gammas in magnitude beyond 20 R _E. It appears that there exists a region of turbulent magnetic fields between the geomagnetic field boundary near 10 R _E, and another boundary, located near 14–15 R _E near the earth-sun line. This second boundary was seen only by Pioneer I and Pioneer V; Explorer XII and Explorer X apparently did not reach it. This boundary has been tentatively identified as a shock front in the flow of solar plasma about the magnetosphere (see Figure 5).^{41, 42} The geomagnetic field inside the boundary is relatively quiet. An abrupt transition in the magnitude of fluctuations occurs at the boundary surface. The ratio of fluctuation amplitude, B, to average field, B, decreases from 1 to 0.1 on a passage through the boundary on 13 September 1961.⁴³ The boundary is not unstable in the solar wind but fluctuations in solar wind pressure do cause changes in boundary location.^42,43 The ring current location appears to be above 1.4 R _E and below 5 R _E on the basis of Pioneer I, Vanguard III, and Explorer XII data. Lunik I and II records indicate that it is located between 3 and 4 R _E. Explorer VI data indicates that it must be at distances greater than 4 R _E on the dark side of the earth. Some variation in altitude of a ring current with time appears likely, but the bulk of present evidence limits a possible ring current to a distance of 3 to 5 R _E.The interplanetary field during quiet times is of the order of 2 to 5 gammas. The direction indicated for this field, with a significant component perpendicular to the earth-sun line, is puzzling in view of solar cosmic ray transit times. Solar disturbances with resultant plasma flow past the satellite produce increases in the field magnitude. Field increases at the satellite are sometimes correlated with disturbances observed at the earth.Further investigations are needed to map the magnetosphere and boundary more completely, to investigate the postulated shock front and the turbulent region inside, to refute or confirm the ring current theory, and to measure the interplanetary field direction and magnitude more completely. Theoretical studies are needed to support these experiments and to suggest new avenues of investigations. Particularly needed are theoretical investigations of collisionless shock fronts in plasma flow and of characteristics of the flow between the shock front and the obstacle.     

Advanced Technologies Demonstrated by the Miniature Integrated Camera and Spectrometer (MICAS) Aboard Deep Space 1

MICAS is an integrated multi-channel instrument that includes an ultraviolet imaging spectrometer (80–185 nm), two high-resolution visible imagers (10–20 μrad/pixel, 400–900 nm), and a short-wavelength infrared imaging spectrometer (1250–2600 nm). The wavelength ranges were chosen to maximize the science data that could be collected using existing semiconductor technologies and avoiding the need for multi-octave spectrometers. It was flown on DS1 to validate technologies derived from the development of PICS (Planetary Imaging Camera Spectrometer). These technologies provided a novel systems approach enabling the miniaturization and integration of four instruments into one entity, spanning a wavelength range from the UV to IR, and from ambient to cryogenic temperatures with optical performance at a fraction of a wavelength. The specific technologies incorporated were: a built-in fly-by sequence; lightweight and ultra-stable, monolithic silicon-carbide construction, which enabled room-temperature alignment for cryogenic (85–140 K) performance, and provided superb optical performance and immunity to thermal distortion; diffraction-limited, shared optics operating from 80 to 2600 nm; advanced detector technologies for the UV, visible and short-wavelength IR; high-performance thermal radiators coupled directly to the short-wave infrared (SWIR) detector optical bench, providing an instrument with a mass less than 10 kg, instrument power less than 10 W, and total instrument cost of less than ten million dollars. The design allows the wavelength range to be extended by at least an octave at the short wavelength end and to ∼50 microns at the long wavelength end. Testing of the completed instrument demonstrated excellent optical performance down to 77 K, which would enable a greatly reduced background for longer wavelength detectors. During the Deep Space 1 Mission, MICAS successfully collected images and spectra for asteroid 9969 Braille, Mars, and comet 19/P Borrelly. The Borrelly encounter was a scientific hallmark providing the first clear, high resolution images and excellent, short-wavelength infrared spectra of the surface of an active comet’s nucleus.