Bacterial community in ancient Siberian permafrost as characterized by culture and culture-independent methods

The microbial composition of ancient permafrost sediments from the Kolyma lowland of Northeast Eurasia was examined through culture and culture-independent approaches. These sediments have been continuously frozen for 5,000 to 2-3 million years. A total of 265 Bacteria 16S rRNA gene sequences were amplified from the permafrost total-community genomic DNA and screened by amplified ribosomal 16S rRNA restriction analysis. Members of three major lineages were found: gamma-Proteobacteria (mostly Xanthomonadaceae), Actinobacteria, and Firmicutes. We also determined partial 16S rRNA gene sequences of 49 isolates from a collection of 462 aerobes isolated from these sediments. The bacteria included Actinomycetales (Arthrobacter and Microbacteriaceae); followed by the Firmicutes (Exiguobacterium and Planomicrobium); the Bacteroidetes (Flavobacterium); the gamma-Proteobacteria (Psychrobacter); and the alpha-Proteobacteria (Sphingomonas). Both culture and culture-independent approaches showed the presence of high and low G+C Gram-positive bacteria and gamma-Proteobacteria. Some of the 16S rRNA gene sequences of environmental clones matched those of Arthrobacter isolates. Two-thirds of the isolates grew at -2.5 degrees C, indicating that they are psychroactive, and all are closely related to phylogenetic groups with strains from other cold environments, mostly commonly from Antarctica. The culturable and non-culturable microorganisms found in the terrestrial permafrost provide a prototype for possible life on the cryogenic planets of the Solar System.     

Consideration in selecting crops for the human-rated life support system: a Linear Programming model.

A Linear Programming model has been constructed which aids in selecting appropriate crops for CELSS (Controlled Environment Life Support System) food production. A team of Controlled Environment Agriculture (CEA) faculty, staff, graduate students and invited experts representing more than a dozen disciplines, provided a wide range of expertise in developing the model and the crop production program. The model incorporates nutritional content and controlled-environment based production yields of carefully chosen crops into a framework where a crop mix can be constructed to suit the astronauts' needs. The crew's nutritional requirements can be adequately satisfied with only a few crops (assuming vitamin mineral supplements are provided) but this will not be satisfactory from a culinary standpoint. This model is flexible enough that taste and variety driven food choices can be built into the model.     

Search for life on Mars: evaluation of techniques.

An important question for exobiology is, did life evolve on Mars? To answer this question, experiments must be conducted on the martian surface. Given current mission constraints on mass, power, and volume, these experiments can only be performed using proposed analytical techniques such as: electron microscopy, X-ray fluorescence, X-ray diffraction, alpha-proton backscatter, gamma-ray spectrometry, differential thermal analysis, differential scanning calorimetry, pyrolysis gas chromatography, mass spectrometry, and specific element detectors. Using prepared test samples consisting of 1% organic matter (bovine serum albumin) in palagonite and a mixture of palagonite, clays, iron oxides, and evaporites, it was determined that a combination of X-ray diffraction and differential thermal analysis coupled with gas chromatography provides the best insight into the chemistry, mineralogy, and geological history of the samples.     

四川烹饪:火辣辣的口味火辣辣的性情

"西餐很乏味,没味没趣,"在成都最好的川菜馆之一蜀风园,侯伟一边吃着清蒸牛蛙一边说:"小孩子倒是喜欢吃西餐,但四川人很挑剔,如果味道不好,我们不会吃."     

Observations of 1–30 MeV gamma rays from the galactic center

Preliminary results are reported for gamma ray observations of the galactic center region made during a 15 hour balloon flight from Alice Springs, Australia on April 18, 1979. The observations were carried out with the UCR double-scatter gamma-ray telescope at energies of 1 to 30 MeV. The observations are compatible with a galactic source of approximately equal brightness along the region 300°<ℓII<60°. The energy distribution joins smoothly to previous spark chamber results at energies above 30 MeV and to scintillator results below 1 MeV. It appears to be a combination of nuclear gamma ray lines superimposed on a bremsstrahlung spectrum with a power law (1.3±.7) × 10⁻³ E⁻(^1.7±.2). The ¹²C* line at 4.4 MeV appears to be present with a significance of about 16σ. The flux in the line is (6±3) × 10⁻⁴photons cm⁻²s⁻¹rad⁻¹. The oxygen line at 6.1 MeV does not seem to appear significantly above background.     

Understanding high-density matter through analysis of surface spectral lines and burst oscillations from accreting neutron stars

We discuss millisecond period brightness oscillations and surface atomic spectral lines observed during type I X-ray bursts from a neutron star in a low mass X-ray binary system. We show that modeling of these phenomena can constrain models of the dense cold matter at the cores of neutron stars. We demonstrate that, even for a broad and asymmetric spectral line, the stellar radius-to-mass ratio can be inferred to better than 5%. We also fit our theoretical models to the burst oscillation data of the low mass X-ray binary XTE J1814-338, and find that the 90% confidence lower limit of the neutron star’s dimensionless radius-to-mass ratio is 4.2.     

Theory of Errors of a Multigimbal, Elastically Supported, Tuned Gyroscope

An elastically supported, tuned gyroscope is a complex device, but experience has shown that it can be represented by mathematical models by means of which practically all of its characteristic errors can be analyzed and predicted with good accuracy. The gyro angular transfer function, derived in [1], serves as an excellent basis of evaluation of errors due to asymmetries of the gimballing system and effects of rotor angular offsets. The gyro translational transfer function, derived in this paper, may be used in the analysis of errors caused by linear acceleration inputs.     

Missile autopilot design using quasi-LPV polynomial eigenstructure assignment

This paper presents the design of a missile autopilot over its flight envelop using quasi-linear parameter-varying polynomial eigenstructure assignment (PEA). The paper describes the extension of PEA to parameter-varying systems using a nonlinear missile model developed by Horton as an example. The autopilot is designed for a single-plane lateral acceleration control and a 5 degree of freedom (DOF) autopilot is also designed. Both lateral acceleration and augmented lateral acceleration outputs are considered. The lateral acceleration autopilot has nonminimum phase characteristics, and it is shown that the quasi-linear parameter-varying PEA approach can handle nonminimum phase systems unlike classic dynamic inversion techniques. Simulation results are presented over fast variations in Mach number and show that the design is robust.     

Interference-free wind tunnel testing of jet propelled missiles

Wind tunnel evaluation of the aerodynamic interaction effects between plume and the external flow past the missile body including the wake boundary (“slipstream”) over powered flight envelopes of rocket propelled vehicles can be greatly facilitated — or even made possible — by a methodology replacing the hot propellant by cold, inert gases. Model nozzle design is based on the second order matching of plume geometry and first order modeling of plume stiffness. Since modeled nozzles will have larger throat radii than the prototypes, one can use sting-supported, sting-fed model installations thus eliminating aerodynamic interference effects due to struts. The concepts of simulated altitude and simulated full-scale Reynolds Number greatly reduce wind tunnel occupancy time. Computer programs, covering all steps of evaluating prototype nozzle performance, model sting nozzle design, model test evaluation and interpretation have been developed. The modeling methodology is supported by experimental results obtained in an induction wind tunnel at the FFA, Bromma, Sweden and in the 16 T and VKF-A altitude tunnels at AEDC, Tullahoma, Tenn.