Morphology: an ambiguous indicator of biogenicity

This paper deals with the difficulty of decoding the origins of natural structures through the study of their morphological features. We focus on the case of primitive life detection, where it is clear that the principles of comparative anatomy cannot be applied. A range of inorganic processes are described that result in morphologies emulating biological shapes, with particular emphasis on geochemically plausible processes. In particular, the formation of inorganic biomorphs in alkaline silica-rich environments are described in detail.     

The theoretical advantages and practical considerations of gas replenishment techniques in long-duration scientific ballooning

Long-duration scientific balloon flights using conventional ballasting methods to compensate for daily loss of lift suffer a severe payload weight penalty. Typically, the weight of the expendable ballast approaches or exceeds the weight of the balloon and payload. This paper examines the concept of correcting for degraded lift by “gas replenishment” techniques, that is, by replacing lost helium inflatant from an on-board dewar. Appropriate equations are developed and curves are presented which demonstrate the clear theoretical superiority of the replenishment method. Design problems associated with the dewars and heat exchangers required to implement the gas replenishment concept are also discussed in detail. A series of curves is presented in which net load, dewar weight and quantity of helium required are plotted for specific balloons and float altitudes as a function of daily loss rate. The lack of relevant heat exchange data in the literature is cited as an obstacle to immediate prototype development.     

Non-radial pulsation models of the H-alpha profile in BW vulpeculae

The profile of the Hydrogen alpha spectral line in the largeamplitude Beta Cephei star BW Vulpeculae was modeled in terms of a linear, non-radially moving, and rotating stellar atmosphere. The pulsation and rotation parameters were derived from fitting the radial velocity amplitude, and the best fit was obtained for =2, m=-2, ¿=90°, rotation velocity=120 km/sec, and pulsation amplitude=27 km/sec. When the line profiles are compared to this model, the fit is quite encouraging for most phases, but several discrepancies remain. At the time of writing, it is not clear whether these can be made to disappear by increasing the sophistication of the model, or whether a completely different physical model (e.g. radial) is necessary.     

Effects of a simulated martian UV flux on the cyanobacterium, Chroococcidiopsis sp. 029

Dried monolayers of Chroococcidiopsis sp. 029, a desiccation-tolerant, endolithic cyanobacterium, were exposed to a simulated martian-surface UV and visible light flux, which may also approximate to the worst-case scenario for the Archean Earth. After 5 min, there was a 99% loss of cell viability, and there were no survivors after 30 min. However, this survival was approximately 10 times higher than that previously reported for Bacillus subtilis. We show that under 1 mm of rock, Chroococcidiopsis sp. could survive (and potentially grow) under the high martian UV flux if water and nutrient requirements for growth were met. In isolated cells, phycobilisomes and esterases remained intact hours after viability was lost. Esterase activity was reduced by 99% after a 1-h exposure, while 99% loss of autofluorescence required a 4-h exposure. However, cell morphology was not changed, and DNA was still detectable by 4',6-diamidino-2-phenylindole staining after an 8-h exposure (equivalent to approximately 1 day on Mars at the equator). Under 1 mm of simulant martian soil or gneiss, the effect of UV radiation could not be detected on esterase activity or autofluorescence after 4 h. These results show that under the intense martian UV flux the morphological signatures of life can persist even after viability, enzymatic activity, and pigmentation have been destroyed. Finally, the global dispersal of viable, isolated cells of even this desiccation-tolerant, ionizing-radiation-resistant microorganism on Mars is unlikely as they are killed quickly by unattenuated UV radiation when in a desiccated state. These findings have implications for the survival of diverse microbial contaminants dispersed during the course of human exploratory class missions on the surface of Mars.     

Bacillus subtilis spore survival and expression of germination-induced bioluminescence after prolonged incubation under simulated Mars atmospheric pressure and composition: implications for planetary protection and lithopanspermia

Bacterial endospores in the genus Bacillus are considered good models for studying interplanetary transfer of microbes by natural or human processes. Although spore survival during transfer itself has been the subject of considerable study, the fate of spores in extraterrestrial environments has received less attention. In this report we subjected spores of a strain of Bacillus subtilis, containing luciferase resulting from expression of an sspB-luxAB gene fusion, to simulated martian atmospheric pressure (7-18 mbar) and composition (100% CO(2)) for up to 19 days in a Mars simulation chamber. We report here that survival was similar between spores exposed to Earth conditions and spores exposed up to 19 days to simulated martian conditions. However, germination-induced bioluminescence was lower in spores exposed to simulated martian atmosphere, which suggests sublethal impairment of some endogenous spore germination processes.     

The NASA Astrobiology Roadmap

The NASA Astrobiology Roadmap provides guidance for research and technology development across the NASA enterprises that encompass the space, Earth, and biological sciences. The ongoing development of astrobiology roadmaps embodies the contributions of diverse scientists and technologists from government, universities, and private institutions. The Roadmap addresses three basic questions: how does life begin and evolve, does life exist elsewhere in the universe, and what is the future of life on Earth and beyond? Seven Science Goals outline the following key domains of investigation: understanding the nature and distribution of habitable environments in the universe, exploring for habitable environments and life in our own Solar System, understanding the emergence of life, determining how early life on Earth interacted and evolved with its changing environment, understanding the evolutionary mechanisms and environmental limits of life, determining the principles that will shape life in the future, and recognizing signatures of life on other worlds and on early Earth. For each of these goals, Science Objectives outline more specific high priority efforts for the next three to five years. These eighteen objectives are being integrated with NASA strategic planning.     

The Urey instrument: an advanced in situ organic and oxidant detector for Mars exploration

The Urey organic and oxidant detector consists of a suite of instruments designed to search for several classes of organic molecules in the martian regolith and ascertain whether these compounds were produced by biotic or abiotic processes using chirality measurements. These experiments will also determine the chemical stability of organic molecules within the host regolith based on the presence and chemical reactivity of surface and atmospheric oxidants. Urey has been selected for the Pasteur payload on the European Space Agency's (ESA's) upcoming 2013 ExoMars rover mission. The diverse and effective capabilities of Urey make it an integral part of the payload and will help to achieve a large portion of the mission's primary scientific objective: "to search for signs of past and present life on Mars." This instrument is named in honor of Harold Urey for his seminal contributions to the fields of cosmochemistry and the origin of life.     

Daytime Ionosphere Retrieval Algorithm for the Ionospheric Connection Explorer (ICON)

The NASA Ionospheric Connection Explorer Extreme Ultraviolet spectrograph, ICON EUV, will measure altitude profiles of the daytime extreme-ultraviolet (EUV) OII emission near 83.4 and 61.7 nm that are used to determine density profiles and state parameters of the ionosphere. This paper describes the algorithm concept and approach to inverting these measured OII emission profiles to derive the associated \(\mathrm{O}^{+}\) density profile from 150–450 km as a proxy for the electron content in the F-region of the ionosphere. The algorithm incorporates a bias evaluation and feedback step, developed at the U.S. Naval Research Laboratory using data from the Special Sensor Ultraviolet Limb Imager (SSULI) and the Remote Atmospheric and Ionospheric Detection System (RAIDS) missions, that is able to effectively mitigate the effects of systematic instrument calibration errors and inaccuracies in the original photon source within the forward model. Results are presented from end-to-end simulations that convolved simulated airglow profiles with the expected instrument measurement response to produce profiles that were inverted with the algorithm to return data products for comparison to truth. Simulations of measurements over a representative ICON orbit show the algorithm is able to reproduce hmF2 values to better than 5 km accuracy, and NmF2 to better than 12% accuracy over a 12-second integration, and demonstrate that the ICON EUV instrument and daytime ionosphere algorithm can meet the ICON science objectives which require 20 km vertical resolution in hmF2 and 18% precision in NmF2.     

Survival of Bacillus subtilis endospores on ultraviolet-irradiated rover wheels and Mars regolith under simulated Martian conditions

Endospores of Bacillus subtilis HA101 were applied to a simulated Mars Exploration Rover (MER) wheel and exposed to Mars-normal UV irradiation for 1, 3, or 6 h. The experiment was designed to simulate a contaminated rover wheel sitting on its landing platform before rolling off onto the martian terrain, as was encountered during the Spirit and Opportunity missions. When exposed to 1 h of Mars UV, a reduction of 81% of viable endospores was observed compared to the non-UV irradiated controls. When exposed for 3 or 6 h, reductions of 94.6% and 96.6%, respectively, were observed compared to controls. In a second experiment, the contaminated rover wheel was rolled over a bed of heat-sterilized Mars analog soil; then the analog soil was exposed to full martian conditions of UV irradiation, low pressure (6.9 mbar), low temperature (-10°C), and an anaerobic CO(2) martian atmosphere for 24 h to determine whether endospores of B. subtilis on the contaminated rover wheel could be transferred to the surface of the analog soil and survive martian conditions. The experiment simulated conditions in which a rover wheel might come into contact with martian regolith immediately after landing, such as is designed for the upcoming Mars Science Laboratory (MSL) rover. The contaminated rover wheel transferred viable endospores of B. subtilis to the Mars analog soil, as demonstrated by 31.7% of samples showing positive growth. However, when contaminated soil samples were exposed to full martian conditions for 24 h, only 16.7% of samples exhibited positive growth-a 50% reduction in the number of soil samples positive for the transferred viable endospores.