A role of cytoskeletal structure of cortical cells in the gravity-regulated formation of a peg in cucumber seedlings.

Seedlings of cucurbitaceous plants develop a protuberant tissue, or peg, on the lower side of the transition region between root and hypocotyl when germinated in a horizontal position. Peg develops due to a change in growth polarity of the cortical cells. We have examined the role of the cytoskeletal structure in peg formation of cucumber seedlings. We observed that in both peg and normal cortical cells of 36 h-old seedlings the microtubules (MTs) were arranged perpendicular to the longitudinal axis of the elongating cells. Application of colchicine perturbed the MTs structure and inhibited the formation of pegs. In 20 h-old seedlings, MTs in cortical cells destined to be a peg tissue had no preferential organization, whereas MTs in normal cortical cells were transversely oriented. After 24 h, the MTs in future peg cells were arranged similar to those of 36 h-old seedlings, although the initiation of peg tissue was not yet visible. These results suggest that reorganization of MTs is required for peg formation and causes the change in growth polarity of the cortical cells.     

Structure and NIR feature of QCC: a laboratory analog of carbon dust.

We have produced thin films of quenched carbonaceous composite (QCC) by hydrocarbon plasma deposition. The effect of thermal annealing on QCC has been investigated to understand how QCC, as a laboratory analog of carbon dust, is transformed in the warm environment around evolved stars. Spectroscopic measurements have indicated that, by heating, the proportion of aromatic sp2 CH bonds increases relative to sp3 CH bonds. Carbon onion-like spherules of approximately 10 nm in diameter are found with electron microscopic images after "graphitization" of thermal annealing.     

Structure and UV absorption of QCCs: carbonaceous dust analogues.

"Quenched Carbonaceous Composites (QCCs)" are carbonaceous interstellar dust analogues synthesized in the laboratory from a hydrocarbon plasma. We produced new types of carbonaceous condensates from the ejecta of plasma with mixtures of methane and hydrogen as source gases. We find that QCC with an absorbance peak at 220 nm is composed of onion-like spherules, and QCCs with an absorbance peak at 230-240 nm are composed of polyhedral particles. The onion-like QCC contains aromatic hydrogen bonds, and it shows the 3.3 and 11.4 micrometers absorption bands. The QCC with an absorbance peak at 230-240 nm is composed of ribbons with bent graphitic layers. This suggests that the carrier of the interstellar 220 nm extinction band might also be an emitter of the interstellar diffuse emission bands.     

Understanding plant-soil relationships using controlled environment facilities.

Although soil is a component of terrestrial ecosystems, it is comprised of a complex web of interacting organisms, and therefore can be considered itself as an ecosystem. Soil microflora and fauna derive energy from plants and plant residues and serve important functions in maintaining soil physical and chemical properties, thereby affecting net primary productivity (NPP), and in the case of contained environments, the quality of the life support system. We have been using 3 controlled-environment facilities (CEF's) that incorporate different levels of soil biological complexity and environmental control, and differ in their resemblance to natural ecosystems, to study relationships among plant physiology, soil ecology, fluxes of minerals and nutrients, and overall ecosystem function. The simplest system utilizes growth chambers and specialized root chambers with organic-less media to study the physiology of plant-mycorrhizal associations. A second system incorporates natural soil in open-top chambers to study soil bacterial and fungal population response to stress. The most complex CEF incorporates reconstructed soil profiles in a "constructed" ecosystem, enabling close examination of the soil foodweb. Our results show that closed ecosystem research is important for understanding mechanisms of response to ecosystem stresses. In addition, responses observed at one level of biological complexity may not allow prediction of response at a different level of biological complexity. In closed life support systems, incorporating soil foodwebs will require less artificial manipulation to maintain system stability and sustainability.     

Effects of repeated long duration +2Gz load on man's cardiovascular function

Usefulness of a short-arm human centrifuge is expected when it is used in space as a countermeasure against cardiovascular deconditioning, problem of bone-calcium metabolism, etc. However, nothing is solidly established regarding the most desirable program for artificial G application. Accordingly, this study was designed to analytically evaluate the effects of repeated long duration +Gz load on human cardiovascular function. Recently heart rate spectral analysis has been recognized as a powerful tool for quantitatively evaluating parasympathetic and sympathetic activity separately in human. It is reported that power of the high frequency component (HF-p) is mediated selectively by parasympathetic activity and the power ratio of low to high frequency components(LF/HF) is indicative of cardiac sympathetic activity or cardiac sympathovagal balance. Sequence method is developed to examine spontaneous baroreceptor reflex sensitivity (BRS). We studied cardiovascular control function by using these methods in 9 healthy men before and after 7 days of daily repeated 1hour +2Gz load. When compared with the data of pre-G load period, post-G load period, decrease of HR, increases of HF-p and BRS were statistically significant. SBP, DBP and LF/HF tended to decrease, however, these changes were not statistically significant. This results indicate that repeated +2Gz load increases parasympathetic activity and arterial baroreceptor-cardiac reflex sensitivity. In recent years, many investigators suggest that space flight and head-down bedrest leads to impaired baroreceptor-cardiac reflex responses and decrease of parasympathetic activity, which may contribute to orthostatic intolerance. So our results suggest that daily repeated 1hour +2Gz load would be useful in preventing post-flight orthostatic intolerance.     

A new preoxygenation procedure for extravehicular activity (EVA)

A 10.2 psi staged-decompression schedule or a 4-hour preoxygenation at 14.7 psi is required prior to extravehicular activity (EVA) to reduce decompression sickness (DCS) risk. Results of recent research at the Air Force Research Laboratory (AFRL) showed that a 1-hour resting preoxygenation followed by a 4-hour, 4.3 psi exposure resulted in 77% DCS risk (N=26), while the same profile beginning with 10 min of exercise at 75% of VO2peak during preoxygenation reduced the DCS risk to 42% (P<.03; N=26). A 4-hour preoxygenation without exercise followed by the 4.3 psi exposure resulted in 47% DCS risk (N=30). The 1-hour preoxygenation with exercise and the 4-hour preoxygenation without exercise results were not significantly different. Elimination of either 3 hours of preoxygenation or 12 hours of staged-decompression are compelling reasons to consider incorporation of exercise-enhanced preoxygenation.     

Six-month space greenhouse experiments--a step to creation of future biological life support systems

SVET Space Greenhouse (SG)--the first automated facility for growing of higher plants in microgravity was designed in the eighty years to be used for the future BLSS. The first successful experiment with vegetables was carried out in 1990 on the MIR Space Station (SS). The experiments in SVET SG were resumed in 1995, when an American Gas Exchange Measurement System (GEMS) was added. A three-month wheat experiment was carried out as part of MIR-SHUTTLE'95 program. SVET-2 SG Bulgarian equipment of a new generation with optimised characteristics was developed (financed by NASA). The new SVET-GEMS equipment was launched on board the MIR SS and a successful six-month experiments for growing up of two crops of wheat were conducted in 1996 - 97 as part of MIR-NASA-3 program. The first of these "Greenhouse" experiments (123 days) with the goal to grow wheat through a complete life cycle is described. Nearly 300 heads developed but no seeds were produced. A second crop of wheat was planted and after 42 days the plants were frozen for biochemical investigations. The main environmental parameters during the six-month experiments in SVET (substrate moisture and lighting period) are given. The results and the contribution to BLSS are discussed.     

Arm end-point trajectories under normal and micro-gravity environments

The purpose of the present experiment was to study the way in which the CNS represents gravitational force during vertical arm pointing movements. Movements in upward and downward directions were executed by two cosmonauts in normal-gravity and weightlessness. Analyses focused upon finger kinematics in the sagittal plane. In normal-gravity, downward direction movements showed smaller curvatures and greater relative times to peak velocity (AT/MT) when compared with upward direction movements. Data from the weightlessness experiments showed that whilst downward movements decreased their curvature during space flight, curvatures of upward movements changed slightly. Furthermore, AT/MT was modified during the first days in micro-gravity for both directions, recovering, however, to pre-flight values after 18 days in space. Results from the present study, provide evidence that gravitational force is centrally treated constituting an important component of the motor plan for vertical arm movements.     

Helium-3: Status and Prospects

We report on our continuing efforts to determine 3He abundances in H II regions and planetary nebulae. Our detections of 3He in some PNe show that some stars produce large amounts of 3He. However the H II region abundances show no evidence for this production. From our sample of > 40 H II regions, the subsample which should yield the most reliable abundances has 3He/H abundances which scatter between 1-2 × 10-5. There is no trend with either galactocentric distance or metallicity. Even if we do not understand the underlying mechanisms, we see empirically that stars neither produce nor destroy 3He in a major way. We thus suggest that the level of the "3He Plateau" (3He/H = 1.5 _-0.5 ^+1.0 × 10^-5) is a reasonable estimate for the primordial 3He.     

First millimetric detections of AE Aquarii

We report the first millimetric detections of the magnetic cataclysmic variable AE Aquarii, accompanied by contemporaneous microwave observations. These data show that the time-averaged spectrum is well fit by a power-law which extends to mm wavelengths. We suggest that the spectrum is consistent with that expected from a superposition of flare-like events.