Evaluation of gravity-dependent membrane potential shift in Paramecium

It is still debated whether or not gravity can stimulate unicellular organisms. This question may be settled by revealing changes in the membrane potential in a manner depending on the gravitational forces imposed on the cell. We estimated the gravity-dependent membrane potential shift to be about 1 mV G⁻¹ for Paramecium showing gravikinesis at 1–5 G, on the basis of measurements of gravity-induced changes in active propulsion and those of propulsive velocity in solutions, in which the membrane potential has been measured electrophysiologically. The shift in membrane potential to this extent may occur from mechanoreceptive changes in K⁺ or Ca²⁺ conductance by about 1% and might be at the limit of electrophysiological measurement using membrane potential-sensitive dyes. Our measurements of propulsive velocity vs membrane potential also suggested that the reported propulsive force of Paramecium measured in a solution of graded densities with the aid of a video centrifuge microscope at 350 G was 11 times as large as that for −29 mV, i.e., the resting membrane potential at [K⁺]_o = 1 mM and [Ca²⁺]_o = 1 mM, and, by extrapolation, that Paramecium was hyperpolarized to −60 mV by gravity stimulation of 100- G equivalent, the value corrected by considering the reduction of density difference between the interior and exterior of the cell in the graded density solution. The estimated shift of the membrane potential from −29 mV to −60 mV by 100- G equivalent stimulation, i.e., 0.3 mV G⁻¹, could reach the magnitude entirely feasible to be measured more directly.     

Physiologic and metabolic responses of wheat seedlings to elevated and super-elevated carbon dioxide

The metabolic consequence of suboptimal (400 μmol mol⁻¹ or ppm), near-optimal (1500 ppm) and supra-optimal (10,000 ppm) atmospheric carbon dioxide concentrations [CO₂] was investigated in an attempt to reveal plausible underlying mechanisms for the differential physiological and developmental responses to increasing [CO₂]. Both non-targeted and targeted metabolite profiling by GC–MS and LC–MS were employed to examine primary and secondary metabolites in wheat (Triticum aestivum, cv Yocoro rojo) continuously exposed to these [CO₂] levels for 14, 21 and 28 days. Metabolite profile was altered by both [CO₂] and physiological age. In general, plants grown under high [CO₂] exhibited a metabolite profile characteristic of older plants under ambient CO₂. Elevated [CO₂] resulted in higher levels of phosphorylated sugar intermediates, though no clear trend in the content of reducing sugars was observed. Transient starch content was enhanced by increasing [CO₂] to a much greater extent at 10,000 ppm CO₂ than at 1500 ppm CO₂. The percentage increase of starch content resulting from CO₂ enrichment declined as plants develope. In contrast, elevated [CO₂] promoted the accumulation of secondary metabolites (flavonoids) progressively to a greater extent as plants became mature. Elevated [CO₂] to 1500 ppm induced a higher initial growth rate, while super-elevated [CO₂] appeared to negate such initial growth promotion. However, after 4 weeks, there was no difference in vegetative growth between 1500 and 10,000 ppm CO₂-grown plants, both elevated CO₂ levels resulted in an overall 25% increase in biomass over the control plants. More interestingly, elevated atmospheric [CO₂] reduced evapotranspiration rate (ET), but further increase to the supra-optimal level resulted in increased ET (a reversed trend), i.e. ET at 1500 ppm < ET at 10,000 ppm < ET at 400 ppm. The differential effect of elevated and super-elevated CO₂ on plants was further reflected in the nitrogen dynamics. These results provide the potential metabolic basis for the differential productivity and stomatal function of plants grown under elevated and super-elevated CO₂ levels.     

Mesospheric H2O and H2O2 densities inferred from in situ positive ion composition measurement

The measurements of positive ion composition in the high latitude D-region have revealed an excess of 34⁺ under distrubed conditions which has been interpreted as H₂O₂⁺. At the same altitude range near the transition height oxonium ions were measured as well. This paper presents a new model for the production and loss of oxonium ions with their production from H₂O₂⁺ + H₂O → H₃O⁺ + HO₂ and their loss by attachment of N₂ and/or CO₂. A reaction constant of 8.5×10^?28 (300/T)⁴ cm⁶s^?1 has been obtained for the three body attachment H₃O⁺ + CO₂ + M → H₃O⁺.CO₂ + M from the measured density profile of 63⁺ in flight 18.1020. Mesospheric H₂O and H₂O₂ densities are inferred from measurements of four high latitude ion compositions based on the oxonium model. The mixing ratios of hydrogen peroxide are up to two orders of magnitude higher compared to previous model calculations. In order to explain the missing production of odd hydrogen, we consider larger O(¹D) densities, surface reactions of O(³P) on particles, and cathalytic photodissociation of water vapor on aerosol particles.     

Mass spectrometric measurements of stratospheric ions

Recent in situ measurements with balloon borne quadrupole mass spectrometers, between 20 and 45 km altitude, are reviewed and discussed.The major stratospheric positive ions observed are proton hydrates [H⁺(H₂O)_n] and non proton hydrates of the form H⁺X_m(H₂O)₂. The data analysis allows a derivation of the vertical mixing ratio profile of X (most probably CH₃CN), which is compared with recent model calculations. From negative ion composition data, showing the presence of NO₃^? and HSO₄^? cluster ions, the density of sulfuric acid in the stratosphere is deduced. The implications of these findings on our understanding of the sulfur chemistry is briefly treated.Finally some other aspects such as contamination, cluster break up and the use of stratospheric ion mass spectra for determination of thermochemical data and other minor constituents are discussed.     

Global comparison of the model results of GSM TIP with IRI for summer conditions

This paper presents the results of the numerical calculations thermosphere/ionosphere parameters which were executed with using of the Global Self-consistent Model of the Thermosphere, Ionosphere and Protonosphere (GSM TIP)and comparison of these results with empirically-based model IRI-2001. Model GSM TIP was developed in West Department of IZMIRAN and solves self-consistently the time-dependent, 3-D coupled equations of the momentum, energy and continuity for neutral particles (O₂, N₂, O), ions (O⁺, H⁺), molecular ions (M⁺) and electrons and largescale eletric field of the dynamo and magnetospheric origin in the range of height from 80 km to 15 Earth’s radii. The empirically derived IRI model describes the E and F regions of the ionosphere in terms of location, time, solar activity and season. Its output provides a global specification not only of N_e but also on the ion and electron temperatures and the ion composition. These two models represent a unique set of capabilities that reflect major differences in along with a substantial approaches of the first-principles model and global database model for the mapping ionosphere parameters. We focus on global distribution of the N_e, T_i, T_e and TEC for the one moment UT and fixed altitudes: 110 km, h_mF2, 300 km and 1000 km. The calculations were executed with using of GSM TIP and IRI models for August 1999, moderate solar activity and quiet geomagnetic conditions. Results present as the global differences between the IRI and GSM TIP models predictions. The discrepancies between model results are discussed.     

Venus EUV measurements of hydrogen and helium from Venera 11 and Venera 12

Lyman α and 58.4 nm HeI radiations resonantly scattered were observed with EUV spectrophotometers flown on Venera 11 and Venera 12. The altitude distribution of hydrogen was derived by limb observations from 250 km (exobase level) to 50,000 km. In the inner exosphere (up to ? 2,000 km of altitude) the distribution can be described by a classical exospheric distribution with T_C = 275 ± 25 K and n = 4_?2⁺³ × 10⁴ atom. cm^?3 at 250 km. The integrated number density from 250 to 110 km (the level of CO₂ absorption) is 2.1 × 10¹² atom. cm^?2, a factor of 3 to 6 lower than that predicted by aeronomical models. This number density decreases from the morning side to the afternoon side, or alternately from equatorial to polar regions. Above 2,000 km a “hot” hydrogen population dominates, which can be simulated by T = 10³K and n = 10³ atom. cm^?3 at the exobase level.The optical thickness of helium above 141 km (the level of CO₂ absorption for 58.4 nm radiation) was determined to be τ_o = 3, corresponding to a density at 150 km of 1.6 × 10⁶ cm^?3. This is about 3 times less than what was obtained with the Bus Neutral Mass Spectrometer of Pioneer Venus, and about twice less than ONMS measurements, but is in agreement with earlier EUV measurement by Mariner 10 (2 ± 1 × 10⁶ cm^?3).     

Molecular analysis of the graviperception signal transduction in the flagellate Euglena gracilis: Involvement of a transient receptor potential-like channel and a calmodulin

Euglena gracilis, a unicellular, photosynthetic flagellate is a model system for environmentally controlled behavior responses. The organism shows pronounced negative gravitaxis. This movement is based on physiological mechanisms, which in the past had been only indirectly assessed. It was shown that mechano-sensitive calcium channels are involved in the gravitaxis response. Recent studies have demonstrated that members of the transient receptor potential (TRP) family function as mechano-sensitive channels in several different cell types. We have sequenced part of a TRP gene in Euglena and applied RNA interference (RNAi) to confirm that these channels are involved in graviperception. It was found that RNAi against the putative TRP channel abolished gravitaxis. The genes of three calmodulins were sequences in Euglena, one of which was previously known in its protein structure (cal 1). The other two were unknown (cal 2 and cal 3). Cal 2 has been analyzed in detail. The biosynthesis of the corresponding proteins of cal 1 and cal 2 was inhibited by means of RNA interference to see whether this blockage impairs gravitaxis. RNAi of cal 1 leads to a long-term loss of free swimming in the cells (while euglenoid movement persists). It induced pronounced cell form aberrations and the division of cells was hampered. After recovery from RNAi the cell showed precise negative gravitaxis again. Thus cal 1 does not seem to be involved in gravitaxis. In contrast, the blockage of cal 2 has no pronounced influence on motility and cell form but leads to a complete loss of gravitactic orientation for more than 30 days showing that this calmodulin is an element in the signal transduction chain. The data are discussed in the context of the current model of the gravitaxis signal transduction chain in Euglena gracilis.     

Sensitivity analysis for air temperature profile estimation methods around the tropopause using simulated Aqua/AIRS data

A sensitivity analysis is performed to investigate potential improvements to the accuracy of air temperature profile retrievals near the tropopause. A simple inversion method is employed to identify and remove redundant spectral channels from the retrievals using simulated data for the high-spectral resolution sounder AIRS (Atmospheric Infrared Sounder) on the Aqua satellite. Bayesian optimal theory and inverse technique are applied for the atmospheric temperature profile retrievals, and the 15 μm CO₂ absorption bands (620–750 cm⁻¹) are chosen for this study. Sequentially elimination of redundant channels is directly integrated into the inverse scheme for the temperature profile, in order to accurately retain the valuable channels and remove all the redundant channels, for accurate retrieval of the temperature profile. Also, the tropopause and troposphere are treated differently in the inverse scheme to improve the retrieval accuracy in the tropopause. Results of a sensitivity analysis based on this method, for the Tropical and Middle-Latitude Summer models simulated by MODTRAN4.0, show that the estimated accuracies are improved by 2 K around the tropopause, and are only changed by less than 0.2 K in the troposphere.     

骨特异性转录因子Runx2对抗空间骨丢失效应的初步研究

为了构建稳定过表达Runx2 (骨特异性转录因子)的C2C12 (小鼠成肌细胞)和MG63 (前成骨细胞)细胞株, 并用于研究Runx2在对抗空间骨丢失效应中的作用. 利用实时定量聚合酶链式反应鉴定Runx2下游基因I型胶原、碱性磷酸酶表达情况, 在二维回转器中培养稳定细胞株, 通过定量聚合酶链式反应,观察在模拟失重效应下Runx2基因对其下游基因表达的影响. 结果表明, 通过筛选获得稳定转染的C2C12-Runx2和MG63-Runx2细胞株, 经鉴定都能过表达Runx2. 转染后的细胞 I 型胶原和碱性磷酸酶mRNA表达增高. 回转组与对照组相比, MG63, C2C12-Runx2, MG63-Runx2细胞的I型胶原和碱性磷酸酶mRNA表达降低, 但在模拟失重效应下, 转染细胞中I型胶原和碱性磷酸酶的mRNA表达下降程度明显低于未转染细胞株. 所构建的C2C12-Runx2和MG63-Runx2细胞株比较稳定, 并证实Runx2能部分对抗失重引起的成骨特异性分子的降低.      

Effects of simulated weightlessness on fish otolith growth: Clinostat versus Rotating-Wall Vessel

Stimulus dependence is a general feature of developing sensory systems. It has been shown earlier that the growth of inner ear heavy stones (otoliths) of late-stage Cichlid fish (Oreochromis mossambicus) and Zebrafish (Danio rerio) is slowed down by hypergravity, whereas microgravity during space flight yields an opposite effect, i.e. larger than 1 g otoliths, in Swordtail (Xiphophorus helleri) and in Cichlid fish late-stage embryos. These and related studies proposed that otolith growth is actively adjusted via a feedback mechanism to produce a test mass of the appropriate physical capacity. Using ground-based techniques to apply simulated weightlessness, long-term clinorotation (CR; exposure on a fast-rotating Clinostat with one axis of rotation) led to larger than 1 g otoliths in late-stage Cichlid fish. Larger than normal otoliths were also found in early-staged Zebrafish embryos after short-term Wall Vessel Rotation (WVR; also regarded as a method to simulate weightlessness). These results are basically in line with the results obtained on Swordtails from space flight.     

Effects of different carbon dioxide and LED lighting levels on the anti-oxidative capabilities of Gynura bicolor DC

Gynura bicolor DC is not only an edible plant but also a kind of traditional Chinese herbal medicine. G. bicolor DC grown in controlled environmental chambers under 3 CO₂ concentrations [450 (ambient), 1500 (elevated), 8000 (super-elevated) μmol mol⁻¹] and 3 LED lighting conditions [white (WL), 85% red + 15% blue (RB15), 70% red + 30% blue (RB30) ] were investigated to reveal plausible antioxidant anabolic responses to CO₂ enrichment and LED light quality. Under ambient and elevated CO₂ levels, blue light increasing from 15% to 30% was conducive to the accumulation of anthocyanins and total flavonoids, and the antioxidant activity of extract was also increased, but plant biomass was decreased. These results demonstrated that the reinforcement of blue light could induce more antioxidant of secondary metabolites, but depress the effective growth of G. bicolor DC under ambient and elevated CO₂ levels. In addition, compared with the ambient and elevated CO₂ levels, the increased anthocyanins, total flavonoids contents and antioxidant enzyme activities of G. bicolor DC under super-elevated CO₂ level could serve as important components of antioxidative defense mechanism against CO₂ stress. Hence, G. bicolor DC might have higher tolerance to CO₂ stress.     

Simulated microgravity inhibits cell wall regeneration of Penicillium decumbens protoplasts

This work compares cell wall regeneration from protoplasts of the fungus Penicillium decumbens under rotary culture (simulated microgravity) and stationary cultures. Using an optimized lytic enzyme mixture, protoplasts were successfully released with a yield of 5.3 × 10⁵ cells/mL. Under simulated microgravity conditions, the protoplast regeneration efficiency was 33.8%, lower than 44.9% under stationary conditions. Laser scanning confocal microscopy gave direct evidence for reduced formation of polysaccharides under simulated conditions. Scanning electron microscopy showed the delayed process of cell wall regeneration by simulated microgravity. The delayed regeneration of P. decumbens cell wall under simulated microgravity was likely caused by the inhibition of polysaccharide synthesis. This research contributes to the understanding of how gravitational loads affect morphological and physiological processes of fungi.     

Comparison of observed hmF2 and IRI 2007 model with M(3000)F2 estimation of hmF2 at low solar activity for an equatorial station

This is to investigate ways of improving the Equatorial F2-layer peak heights estimated from M(3000)F2 ionosonde data measured using the Ionospheric Prediction Service (IPS-42) sounder at Ouagadougou, Burkina Faso (Latitude +12.4°N, Longitude +1.5°W, Dip latitude +5.9°N) during a low solar activity year (1995). For this purpose, we have compared the observed h_mF2 (h_mF2_obs) deduced using an algorithm from scaled virtual heights of quiet day ionograms and the predicted h_mF2 values which is given by the IRI 2007 model (h_mF2_{IRI 2007}) with the ionosonde measured M(3000)F2 estimation of the h_mF2 values (h_mF2_est) respectively. The correlation coefficients R² for all the seasons were found to range from 0.259 to 0.692 for h_mF2_obs values, while it ranges from 0.551 to 0.875 for the h_mF2_{IRI 2007} values. During the nighttime, estimated h_mF2 (h_mF2_est) was found to be positively correlated with the h_mF2_obs values by the post-sunset peak representation which is also represented by the h_mF2_{IRI 2007} values. We also investigated the validity of the h_mF2_est values by finding the percentage deviations when compared with the h_mF2_obs and h_mF2_{IRI 2007}.     

Possible consequences of a disk around B0656+14 on e–e and near-1 Hz gravitational wave production

Nearby pulsars B0656+14 and Geminga were proposed in the literature as the main sources of cosmic-ray positrons observed near Earth above 10 GeV. B0656+14 has comparable distance from Earth, similar magnetic field and period of Geminga. However, observations in the R and I bands indicate the presence of a disk of approximately 10⁻⁴ M_⊙ around B0656+14. Radio and pulsed γ-ray flux observations from this pulsar are also consistent with supernova fallback material and disk entering the light cylinder and partially quenching the development of electromagnetic showers in the magnetosphere. If this is the case, B0656+14 has unlikely given any contribution to e⁺ and e⁻ observed near Earth. Absolute flux measurements and the level of anisotropy in the high energy electron and positron arrival directions above 50 GeV will help in revealing if none, one of both nearby pulsars are sources of these particles observed near Earth.     

Effects of simulated weightlessness on the intestinal mucosal barrier of rats

This study employed a rat tail-suspension model to investigate the effects of simulated weightlessness on the intestinal mucosal barrier. Twenty-four Wistar rats were randomly divided into control (CON), 14-day tail-suspension (SUS-14d), and 21-day tail-suspension (SUS-21d) groups (n = 8 per group). Expression of occludin and zonula occludins-1 (ZO-1), proteins of the tight junction (TJ), in the intestinal mucosa was measured by immunohistochemical analysis, Western blotting, and mRNA fluorescent quantitation PCR. Plasma concentrations of diamine oxidase (DAO) and d-lactate were determined using an enzymatic spectrophotometric assay. Expression of occludin and ZO-1 was reduced in the SUS-14d and SUS-21d groups as compared to the CON group, with lowest expression observed in the SUS-21d group (P < 0.01). Examination by transmission electron microscopy (TEM) of the jejunal epithelium revealed increased intercellular space, decreased TJ and desmosome densities, and destruction of microvilli in the SUS-14d and SUS-21d groups. Plasma DAO and d-lactate concentrations in the SUS-21d group were higher than those in SUS-14d group and significantly higher than those in the CON group (P < 0.01). In all three groups, the expression of occludin and ZO-1 was found to correlate negatively with DAO (P < 0.01) and d-lactate (P < 0.01) concentrations. It is concluded that simulated weightless results in down-regulation of expression of TJ proteins in the rat intestinal mucosa. Simulated weightlessness is proposed to increase intestinal permeability through damage to the TJ.     

Efficacy of oxygen-supplying capacity of Azolla in a controlled life support system

Azolla shows high growth and propagation rates, strong photosynthetic O₂-releasing ability and high nutritional value. It is suitable as a salad vegetable and can be cultured on a multi-layered wet bed. Hence, it possesses potential as a fresh vegetable, and to release O₂ and absorb CO₂ in a Controlled Ecological Life Support System in space. In this study, we investigated the O₂-providing characteristics of Azolla in a closed chamber under manned, controlled conditions to lay a foundation for use of Azolla as a biological component in ground simulation experiments for space applications. A closed test chamber, representing a Controlled Ecological Life Support System including an Azolla wet-culture device, was built to measure the changes in atmospheric O₂ and CO₂ concentrations inside the chamber in the presence of coexisting Azolla, fish and men. The amount of O₂ consumed by fish was 0.0805–0.0831 L kg⁻¹ h⁻¹ and the level of CO₂ emission was 0.0705–0.0736 L kg⁻¹ h⁻¹; O₂ consumption by the two trial volunteers was 19.71 L h⁻¹ and the volume of respiration-released CO₂ was 18.90 L h⁻¹. Under 7000–8000 Lx artificial light and Azolla wet-culture conditions, human and fish respiration and Azolla photosynthesis were complementary, thus the atmospheric O₂ and CO₂ concentrations inside chamber were maintained in equilibrium. The increase in atmospheric CO₂ concentration in the closed chamber enhanced the net photosynthesis efficiency of the Azolla colony. This study showed that Azolla has strong photosynthetic O₂-releasing ability, which equilibrates the O₂ and CO₂ concentrations inside the chamber in favor of human survival and verifies the potential of Azolla for space applications.     

The accuracy of data from ionosondes for the estimation of hmF2 and the identification of global change in the ionosphere

A long temporal series of simulated ionograms was generated with a superimposed secular variation of −14 km/century on the h_mF2 parameter. These ionograms were interpreted by the automatic scaling program Autoscala. By applying four different empirical formulas, four artificial series of h_mF2 were generated and then processed with the same methods used by other authors for real data sets. Data analysis of the simulated ionograms revealed the artificially imposed long-term trend. These results lead to the conclusion, that regardless of the empirical formula used, the accuracy of h_mF2 from ionosonde measurements would be adequate to observe a long-term trend of −14 km/century.     

High-density and high-temperature molecular gas around the AGN in M51

We investigated the physical properties of molecular gas in the nuclear region of M51 (Seyfert 2). We obtained an aperture synthesis ¹³CO(J = 1 − 0) image using the Nobeyama Millimeter Array (NMA), and compared it with NMA ¹²CO(J = 1 − 0) and HCN(J = 1 − 0) maps at similar spatial resolutions. Within a radius of 180 pc from the center, the ¹³CO(1 − 0) integrated intensity was found to be 3 times weaker than that of HCN(1 − 0). Large-Velocity-Gradient (LVG) calculations suggest that the observed high HCN(1 − 0)/¹³CO(1 − 0) intensity ratio would arise from dense (n_H2 ∼ 10⁵ cm⁻³) and hot (T_kin ≳ 300 K) molecular clouds in the nuclear molecular disk. We also observed in the ¹²CO(1 − 0), (3 − 2), ¹³CO(1 − 0), and (3 − 2) lines using the Nobeyama 45m and JCMT 15m telescopes. We detected weak ¹³CO lines as well as strong ¹²CO lines. The LVG calculations assuming a two-component model suggest that there is a large amount of low-density (n_H2 ∼ 3 − 6 × 10² cm⁻³), low-temperature (T_kin ∼ 20 – 50 K) gas, and a small amount of high-density (n_H2 ≳ 10⁴ cm⁻³), high-temperature (T_kin ≳ 500 K) gas. The existence of the high-density and high-temperature component, although having a quite small beam filling factor, supports the aperture synthesis observation results mentioned above. Since this dense, hot gas is located in the nuclear molecular disk around the Active Galactic Nucleus (AGN), it may be heated by the strong X-ray radiation and/or by the shock induced by the radio jet.     

Rotational constants of linear and/or bent Cn+1H and CnN(n = 1–6): A DFT study

The geometries, dipole moments, and rotational constants for the linear and/or bent cations, C_n+1H⁺ and C_nN⁺(n = 1–6), were studied by the B3LYP method with the modest basis sets. For C_nH⁺(n = odd; 3, 5, 7) and C_nN⁺(n = even; 2, 4, 6), the theoretical rotational constants (B_es) of closed-shell singlet C₃H⁺, C₅H⁺, C₇H⁺, CCN⁺, C₄N⁺, and C₆N⁺ were calculated to be about 11,244, 2420, 885.2, 11,970, 2439, and 880.8 MHz, respectively. By contrast, the triplets are stable than the corresponding singlets for C_nH⁺(n = odd; 2, 4, 6) and C_nN⁺(n = even; 3, 5) except CN⁺.