Amphibian egg cytoplasm response to altered g-forces and gravity orientation

Elucidation of dorsal/ventral polarity and primary embryonic axis development in amphibian embryos requires an understanding of cytoplasmic rearrangements in fertile eggs at the biophysical, physiological, and biochemical levels. Evidence is presented that amphibian egg cytoplasmic components are compartmentalized. The effects of altered orientation to the gravitational vector (i.e., egg inversion) and alterations in gravity force ranging from hypergravity (centrifugation) to simulated microgravity (i.e., horizontal clinostat rotation) on cytoplasmic compartment rearrangements are reviewed. The behavior of yolk compartments as well as a newly defined (with monoclonal antibody) non-yolk cytoplasmic compartment, in inverted eggs and in eggs rotated on horizontal clinostats at their buoyant density, is discussed.     

Subcellular components of the amphibian egg: insights provided by gravitational studies.

Most cytoplasmic regions of fertilized amphibian eggs move with respect to the gravity vector in experimentally gravity oriented eggs. The pattern and extent of this movement varies among different batches of eggs. This variation in apparent cytoplasmic viscosity (or, conversely, cytoplasmic mobility) can be correlated with variations in subsequent morphogenesis of experimental, gravitationally manipulated eggs. Therefore, the proper interpretation of gravity experiments with amphibian eggs requires that one understand the subcellular basis for this variation on cytoplasmic mobility. Variation in the packing of the major cytoplasmic organelle, the yolk platelets, or the organization and amount of cytoskeletal components may explain the variation in cytoplasmic mobility. Evidence is presented that the variation in yolk volume density (fraction of total cytoplasmic volume occupied by yolk platelets) does not account for the variation in cytoplasmic mobility in Xenopus laevis eggs. Experimental evidence from cold-shocked inverted eggs indicates that microtubules may be involved in determining cytoplasmic mobility. However, quantitative evidence that the microtubule levels and state of the microtubules (polymerized vs. non-polymerized) in the whole Xenopus laevis egg does not correlate directly with cytoplasmic mobility is presented. The apparent conflict these data represent regarding the role of the cytoskeleton in determining cytoplasmic mobility is discussed.     

Cytoskeleton and gravity at work in the establishment of dorso-ventral polarity in the egg of Xenopus laevis

The establishment of polarities during early embryogenesis is essential for normal development. Amphibian eggs are appropriate models for studies on embryonic pattern formation. The animal-vegetal axis of the axially symmetrical amphibian egg originates during oogenesis and foreshadows the main body axis of the embryo. The dorso-ventral polarity is epigenetically established before first cleavage. Recent experiments strongly suggest that in the monospermic eggs of the anuran Xenopus laevis both the cytoskeleton and gravity act in the determination of the dorso-ventral polarity. In order to test the role of gravity in this process, eggs will be fertilized under microgravity conditions during the SL-D1 flight in 1985. In a fully automatic experiment container eggs will be kept under well-defined conditions and artificially fertilized as soon as microgravity is reached; eggs and embryos at different stages will then be fixed for later examination. Back on earth the material will be analysed and we will know whether fertilization under microgravity conditions is possible. If so, the relation of the dorso-ventral axis to the former sperm entry point will be determined on the whole embryos; in addition eggs and embryos will be analysed cytologically.     

Early development in the mouse: would it be affected by microgravity?

Gravity has been identified as a morphogenetic signal in Amphibian and bird embryonic development so it is plausible that it might be such in mammals as well. Since early mammalian development shows some apparently significant differences to these other groups, a brief summary of mouse embryogenesis will be given identifying events in which polarity is an important feature and consequently, in which gravity may be a causative factor. These include compaction and polarization during cleavage, establishment of the radial axis, the embryonic-abembryonic axis, the dorso-ventral axis, and the anterior-posterior axis, implantation, and the later rotation of the embryo. The experimental data on these morphogenetic steps will be discussed and an assessment of the possible involvement of gravity will be made.     

Quantitative analysis of mutation and selection in self-replicating RNA.

Mutation and selection as principles of Darwinian evolution have contributed a wealth to qualitative insight and understanding of complex biological organizations. However, for quantitative measurements of Darwinian evolution, only model systems are sufficiently simple to allow calculation of values for the relevant evolution parameters. The model system used for our study comprises short-chained RNA species whose self-replication is catalyzed by Q beta replicase. In this system, phenotypic expression of a genotype is reduced to its efficiency in directing its own synthesis. The mechanism of single-stranded RNA reproduction is well understood: RNA synthesis profiles can be described by compact equations. The selection behaviour of competing RNA species can be precisely predicted, using these equations, from kinetic parameters of the species: at low concentrations, RNA species are selected for overall growth rate (fecundity), at higher concentrations, for rapid binding of replicase (selection for competition), and at still higher concentrations, for minimizing losses caused by formation of inactive double strands. Finally, an ecosystem may be established where the different species coexist, their relative concentrations being functions of their kinetic parameters. The analysis of competition and selection can be extended to mutants of a species. Experimental conditions can be found where quantitative measurement of mutation rates and selective values of mutants is possible. The interplay of mutation and selection results in establishing a quasispecies distribution where mutants are represented according to their rates of mutational formation and their selective values. Replicating RNA clones, when amplified, rapidly build up quasispecies distributions containing pronounced "hot spots", produced predominantly by error propagation of nearly neutral mutants. The primitive model system shows the same complex Darwinian behaviour as observed in evolution of biological systems. In the absence of extraneously added template, Q beta replicase synthesizes after long lag times self-replicating RNA de novo. In a first step, nucleoside triphosphates are condensed randomly; self-replicating templates produced by chance are amplified and optimized.     

级间开缝间隙传感器动态测试方法研究

针对某型号分离面间隙传感器的测试要求,提出了一种间隙位移传感器的动态测试方案,设计了测量系统,通过激光测试法测量并记录级间开缝间隙传感器CI1-27在自由弹出状态下的输出响应。经应用,该方法操作便捷,数据可靠,为间隙传感器的动态测试提供了一种新的参考。     

Genetic changes in mammalian cells transformed by helium ions.

Midterm Syrian Hamster embryo (SHE) cells were employed to study high LET-radiation induced tumorigenesis. Normal SHE cells (secondary passage) were irradiated with accelerated helium ions at an incident energy of 22 MeV/u (9-10 keV/micrometer). Transformed clones were isolated after growth in soft agar of cells obtained from the foci of the initial monolayer plated postirradiation. To study the progression process of malignant transformation, the transformed clones were followed by monolayer subculturing for prolonged periods of time. Subsequently, neoplasia tests in nude mice were done. In this work, however, we have focused on karyotypic changes in the banding patterns of the chromosomes during the early part of the progressive process of cell transformation for helium ion-induced transformed cells.     

Dissipative structure and weak turbulence in the solar corona

We have analysed, for the first time, the high resolution X-ray images of the solar corona, obtained by the Yohkoh Mission, as non-linear extended systems. To quantify the spatio-temporal complexity in this extended system, we use an asymmetric spatial fragmentation parameter computed from a matrix representing an image. Choosing different spatial scales on the same image, wave numbers are computed from the intensity contours and this yields a fragmentation spectrum. This spectrum is used to analyze the images of a complex transient event obtained by the Soft X-ray Telescope board the Yohkoh satellite. The dynamics of the fine structures of the contours suggests the origin of the observed fragmentation to be localized weak turbulence process occuring in an evolving coronal active region.     

Modeling medium-scale TEC structures,observed by Belgian GPS receivers network

GALOCAD project “Development of a Galileo Local Component for the nowcasting and forecasting of atmospheric disturbances affecting the integrity of high precision Galileo applications” aims to perform a detailed study on ionospheric small- and medium-scale structures and to assess the influence of these structures on the reliability of Galileo precise positioning applications. GPS-derived TEC (total electron content) is obtained from the Belgium Dense Network (BDN), consisting of 67 permanent GPS stations. An empirical 3-D model is developed for studying these ionospheric structures. The model, named LLT model, described temporal variations of TEC in latitude/longitude frame (46°, 52°)N and (−1°, 11°)E. The spatial variations of TEC are modeled by Tchebishev base functions, while the temporal variations are described by a trigonometric basis. To fit the model to the data, the observed area is divided into bins with (1° × 1°) geographic scale and 6 min on time axis. LLT model is made flexible, with varying number of coefficients along each axis. This allows different degree of smoothing, which is the key element of the present approach. Model runs with higher number of coefficients, capturing in details medium-scale TEC structures are subtracted from results obtained with smaller number of coefficients; the latter represent the background ionosphere. The residual structures are localized and followed as they travel across the observed area. In this way, the size, velocity, and direction of the irregular structures are obtained.     

重力波波包相互作用时空演变的数值研究

本文设计了一种研究二维空间中重力波波包共振相互作用演变的数值模式.利用此模式获得二个大振幅重力波波包通过碰撞而发生的完整的相互作用过程.数值结果表明,能量上行重力波可以通过波-波相互作用激发能量下行重力波;在相互作用的时空演变过程中,3个波包的能量收支情况和相互作用的强度具有局地性;由于考虑了空间传播效应,相互作用过程不再具有周期性,而是出现一个具有不同物理含义的相互作用特征时间,这个新的相互作用特征时间和相互作用最终的发展程度不仅由相互作用系数和初始波包振幅决定,还受到波包空间尺度和波包群速度的相对大小控制.      

Paramecium--a model system for studying cellular graviperception.

Experiments under varied gravitational accelerations as well as in density-adjusted media showed that sensation of gravity in protists may be linked to the known principles of mechanosensation. Paramecium, a ciliate with clear graviresponses (gravitaxis and gravikinesis) is an ideal model system to prove this hypothesis since the ciliary activity and thus the swimming behaviour is controlled by the membrane potential. It has also been assumed that the cytoplasmic mass causes a distinct stimulation of the bipolarly distributed mechano-sensitive K+ and Ca2+ ion channels in the plasma membrane in dependence of the spatial orientation of the cell. In order to prove this hypothesis, different channel blockers are currently under investigation. Gadolinium did not inhibit gravitaxis in Paramecium, showing that it does not specifically block gravireceptors. Further studies concentrated on the question of whether second messengers are involved in the gravity signal transduction chain. Exposure to 5 g for up to 10 min led to a significant increase in cAMP.     

Wobble of a spin stabilized satellite with cross products of inertia and magnetic attitude control

Asymmetrical spin stabilized satellite dynamics in the vicinity of the required motion is considered. The principal axis of the maximum moment of inertia slightly deviates from its assumed direction in the satellite reference frame. This is formalized in the cross products of inertia. This inertial uncertainty results in a wobble, that is undesired angular velocity components perpendicular to the rotation axis, and oscillations of this axis near the required direction. The torque-free motion is investigated first. Expressions that explicitly relate satellite inertia parameters to wobble are provided. Wobble evolution under the action of magnetic damping control is analyzed next. Its amplitude approximate exponential decay behavior and residual unavoidable wobble level are derived. These expressions are compared with numerical simulation results of nonlinear equations of motion including various disturbance sources.     

Application of small-size antennas for estimation of angles of arrival of HF signals scattered by ionospheric irregularities

A modification of the Doppler Interferometry Technique is suggested to enable estimating angles of arrival of comparatively broadband HF signals scattered by random irregularities of the ionospheric plasma with the use of small-size weakly directional antennas. The technique is based on the measurements of cross-spectra phases of the probe radiation recorded at least in three spatially separated points. The developed algorithm has been used to investigate the angular and frequency-time characteristics of HF signals propagating at frequencies above the maximum usable one (MUF) for the direct radio path Moscow-Kharkiv. The received signal spectra show presence of three families of spatial components attributed, respectively, to scattering by plasma irregularities near the middle point of the radio path, ground backscatter signals and scattering of the sounding signals by the intense plasma turbulence associated with auroral activations. It has been shown that the regions responsible for the formation of the third family components are located well inside the auroral oval. The drift velocity and direction of the auroral ionosphere plasma have been determined. The obtained estimates are consistent with the classical conception of the ionospheric plasma convection at high latitudes and do not contradict the results of investigations of the auroral ionosphere dynamics using the SuperDARN network.     

Gravitaxis and graviperception in flagellates.

There is strong evidence that gravitactic orientation in flagellates and ciliates is mediated by an active physiological gravireceptor rather than by passive alignment of the cells in the water column. In flagellates the threshold for graviorientation was found to be at 0.12 x g on a slow rotating centrifuge during the IML-2 mission on the Shuttle Columbia and a subsequent parabolic rocket flight (TEXUS). During the IML-2 mission no adaptation to microgravity was observed over the duration of the space flight, while gravitaxis was lost in a terrestrial closed environmental system over the period of almost two years. Sedimenting statoliths are not likely to be involved in graviperception because of the small size of the cells and their rotation around the longitudinal axis during forward locomotion. Instead the whole cytoplasmic content of the cell, being heavier than the surrounding aqueous medium (1.05 g/ml), exerts a pressure on the lower membrane. This force activates stretch-sensitive calcium specific ion channels which can be inhibited by the addition of gadolinium which therefore abolishes gravitaxis. The channels seem to mainly allow calcium ions to pass since gravitaxis is blocked by the addition of the calcium ionophore A23187 and by vanadate which blocks the Ca-ATPase in the cytoplasmic membrane. Recently, a gene for a mechanosensitive channel, originally sequenced for Saccharomyces, was identified in Euglena by PCR. The increase in intracellular free calcium during reorientation can be visualized by the fluorophore Calcium Crimson using laser excitation and image intensification. This result was confirmed during recent parabolic flights. The gated calcium changes the membrane potential across the membrane which may be the trigger for the reorientation of the flagellum. cAMP plays a role as a secondary messenger. Photosynthetic flagellates are suitable candidates for life support systems since they absorb CO2 and produce oxygen. Preliminary experiments are discussed.     

气膜孔轴线方向的视觉测量技术研究

针对高压涡轮叶片气膜孔的轴线方向测量难题,将非接触式的视觉测量技术与常规的多轴坐标测量技术相结合,搭建了气膜孔五轴视觉坐标测量系统,以在气膜孔的测量方法和设备方面开展探索与研究。为了获取被测气膜孔的轴线矢量参数,提出了采用景深合成技术来实现气膜孔形貌的三维重建,并将工业相机获取到的图像序列转化为三维物理数据的测量方法,而后通过空间直线拟合获取到了被测气膜孔的轴线矢量,以表征气膜孔的轴线方向。为了验证该方法的有效性和可行性,选取某型高压涡轮导向叶片作为被测物体,应用该测量系统对其前缘部位上的目标气膜孔进行了轴线矢量参数的10次等精度重复测量试验,轴线角度的重复性测量误差不大于0.30°,从而提供了一种气膜孔轴线方向的测量技术解决方案。     

Distribution and variability of accelerated electrons at Mars

We investigate accelerated electrons observed by Mars Global Surveyor (MGS), using data from the Electron Reflectometer (ER) instrument. We find three different types of accelerated electron events. Current sheet events occur over regions with weak or no crustal fields, have the highest electron energy fluxes, and are likely located on draped magnetotail fields. Extended events occur over regions with moderate crustal magnetic fields, and are most often observed on closed magnetic field lines. Localized events have the lowest energy fluxes, occur in strong magnetic cusp regions, and are the most likely kind of event to be found on open magnetic field lines. Some localized events have clear signatures of field-aligned currents; these events have much higher electron fluxes, and are preferentially observed on radially oriented open magnetic field lines. Electron acceleration events, especially localized events, are similar in many ways to events observed in the terrestrial auroral zone. However, physical processes related to those found in the terrestrial cusp and/or plasmasheet could also be responsible for accelerating electrons at Mars.     

Studying the evolution of the hot universe with the X-ray evolving universe spectroscopy mission – XEUS

Europe is one of the major partners building the International Space Station (ISS) and European industry, together with ESA, is responsible for many station components including the Columbus Orbital Facility, the Automated Transport Vehicle, two connecting modules and the European Robotic Arm. Together with this impressive list of contributions there is a strong desire within the ESA Member States to benefit from this investment by utilizing the unique capabilities of the ISS to perform world-class science. XEUS is one of the astronomical applications being studied by ESA to utilize the capabilities of the ISS. XEUS will be a long-term X-ray observatory with an initial mirror area of 6 m² at 1 keV that will be expanded to 30 m² following a visit to the ISS. The 1 keV spatial resolution is expected to be 2–5″ half-energy-width. XEUS will consist of separate detector and mirror spacecraft (MSC) aligned by active control to provide a focal length of 50 m. A new detector spacecraft, complete with the next generation of instruments, will also be added after visiting the ISS. The limiting 0.1–2.5 keV sensitivity will then be 4 × 10⁻¹⁸ erg cm⁻² s⁻¹, around 200 times better than XMM-Newton, allowing XEUS to study the properties of the hot baryons and dark matter at high redshift.     

Analysis of Lageos' altitude decrease

The paper treats the inverse problem of celestial mechanics which consists of determining the force field or potential from given or observed orbit(s). From the observational information, according to which Lageos loses approximately 1 mm altitude per day, a linear partial differential equation is formulated. The solution of this equation gives the field responsible for the above-mentioned, as yet unexplained, small but well established secular decrease in the semi-major axis. Note that the altitude-loss is not due to air-drag because of the very high altitude of this satellite.     

Insects as test systems for assessing the potential role of microgravity in biological development and evolution.

Gravity and radiation are undoubtedly the two major environmental factors altered in space. Gravity is a weak force, which creates a permanent potential field acting on the mass of biological systems and their cellular components, strongly reduced in space flights. Developmental systems, particularly at very early stages, provide the larger cellular compartments known, where the effects of alterations in the size of the gravity vector on living organisms can be more effectively tested. The insects, one of the more highly evolved classes of animals in which early development occurs in a syncytial embryo, are systems particularly well suited to test these effects and the specific developmental mechanisms affected. Furthermore, they share some basic features such as small size, short life cycles, relatively high radio-resistance, etc. and show a diversity of developmental strategies and tempos advantageous in experiments of this type in space. Drosophila melanogaster, the current biological paradigm to study development, with so much genetic and evolutionary background available, is clearly the reference organism for these studies. The current evidence on the effects of the physical parameters altered in space flights on insect development indicate a surprising correlation between effects seen on the fast developing and relatively small Drosophila embryo and the more slowly developing and large Carausius morosus system. In relation to the issue of the importance of developmental and environmental constraints in biological evolution, still the missing link in current evolutionary thinking, insects and space facilities for long-term experiments could provide useful experimental settings where to critically assess how development and evolution may be interconnected. Finally, it has to be pointed out that since there are experimental data indicating a possible synergism between microgravity and space radiation, possible effects of space radiation should be taken into account in the planning and evaluation of experiments designed to test the potential role of microgravity on biological developmental and evolution.