Low-dose carbon ion irradiation effects on DNA damage and oxidative stress in the mouse testis

To investigate the effects of low-dose carbon ion irradiation on reproductive system of mice, the testes of outbred Kunming strain mice were whole-body irradiated with 0, 0.05, 0.1, 0.5 and 1 Gy, respectively. We measured DNA double-strand breaks (DNA DSBs) and oxidative stress parameters including malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and testis weight and sperm count at 12 h, 21 d and 35 d after irradiation in mouse testis. At 12 h postirradiation, a significant increase in DNA DSB level but no pronounced alterations in MDA content or SOD activity were observed in 0.5 and 1 Gy groups compared with the control group. At 21 d postirradiation, there was a significant reduction in sperm count and distinct enhancements of DSB level and MDA content in 0.5 and 1 Gy groups in comparison with control. At 35 d postirradiation, the levels of DNA DSBs and MDA, and SOD activity returned to the baseline except for the MDA content in 1 Gy (P < 0.05), while extreme falls of sperm count were still observed in 0.5 (P < 0.01) and 1 Gy (P < 0.01) groups. For the 0.05 or 0.1 Gy group, no differences were found in DNA DSB level and MDA content between control and at 12 h, 21 d and 35 d after irradiation, indicating that lower doses of carbon ion irradiation have no significant influence on spermatogenesis processes. In this study, male germ cells irradiated with over 0.5 Gy of carbon ions are difficult to repair completely marked by the sperm count. Furthermore, these data suggest that the deleterious effects may be chronic or delayed in reproductive system after whole-body exposure to acute high-dose carbon ions.     

The application of micro-CT in monitoring bone alterations in tail-suspended rats in vivo

Osteopenia is a pathological process that affects human skeletal health not only on earth but also in long-time spaceflight. Micro-computed tomography (micro-CT) is a nondestructive method for assessing both bone quantity and bone quality. To investigate the characteristics of micro-CT on evaluating the microgravity-induced osteopenia (e.g. early detection time and the sensitive parameters), the bone loss process of tail-suspended rats was monitored by micro-CT in this study. 8-Week-old female Sprague Dawley rats were divided into two groups: tail suspension (TS) and control (CON). Volumetric bone mineral density (vBMD) and microstructure of the femur and tibia were evaluated in vivo by micro-CT at 0, 7, 14, 22 days. Biomechanical properties of the femur and tibia were determined by three-point bending test. The ash weight of bone was also investigated. The results showed that (1) bone loss in the proximal tibia appeared earlier than in the distal femur. (2) On day 7, the percent bone volume (BV/TV) of the tibia 15.44% decreased significantly, and the trabecular separation (Tb.Sp) 30.29% increased significantly in TS group, both of which were detected earlier than other parameters. (3) Biomechanical properties (e.g. femur, −22.4% maximum load and −23.75% Young’s modulus vs. CON) and ash weight of the femur and tibia decreased significantly in the TS group in comparison to CON group. (4) vBMD of the femur and tibia were clearly related to bone ash and dry weight (r = 0.75–0.87, p < 0.05). (5) BV/TV of both femur and tibia were clearly related to maximum load and Young’s modulus (r = 0.66–0.87, p < 0.05). Similarly, trabecular vBMD and BV/TV of the femur and tibia were clearly related to Young’s modulus (r = 0.73–0.89, p < 0.05). These indicated that BV/TV and Tb.Sp were more sensitive than other parameters for evaluating bone loss induced by tail suspension, moreover, trabecular vBMD and other parameters might be used to evaluate bone strength. Therefore, micro-CT is a reliable and sensitive method for predicting unloading-induced bone loss in small animals.     

Korean space food development: Ready-to-eat Kimchi, a traditional Korean fermented vegetable,sterilized with high-dose gamma irradiation

Addition of calcium lactate and vitamin C, a mild heating, deep-freezing, and gamma irradiation at 25 kGy were conducted to prepare Kimchi as a ready-to-eat space food. It was confirmed that the space food was sterilized by an irradiation at 25 kGy through incubation at 37 °C for 30 days. The hardness of the Space Kimchi (SK) was lower than the untreated Kimchi (CON), but higher than the irradiated Kimchi (IR). Also, this result was supported by the scanning electron microscopic observation. Sensory attributes of the SK were similar to CON, and maintained during preservation at 35 °C for 30 days. According to the Ames test, Kimchi sterilized with a high-dose irradiation exerted no mutagenic activity in the bacterial strains of Salmonella typhimurium. And, the SK was certificated for use in space flight conditions during 30 days by the Russian Institute of Biomedical Problems.     

Effects of simulated weightlessness on fish otolith growth: Clinostat versus Rotating-Wall Vessel

Stimulus dependence is a general feature of developing sensory systems. It has been shown earlier that the growth of inner ear heavy stones (otoliths) of late-stage Cichlid fish (Oreochromis mossambicus) and Zebrafish (Danio rerio) is slowed down by hypergravity, whereas microgravity during space flight yields an opposite effect, i.e. larger than 1 g otoliths, in Swordtail (Xiphophorus helleri) and in Cichlid fish late-stage embryos. These and related studies proposed that otolith growth is actively adjusted via a feedback mechanism to produce a test mass of the appropriate physical capacity. Using ground-based techniques to apply simulated weightlessness, long-term clinorotation (CR; exposure on a fast-rotating Clinostat with one axis of rotation) led to larger than 1 g otoliths in late-stage Cichlid fish. Larger than normal otoliths were also found in early-staged Zebrafish embryos after short-term Wall Vessel Rotation (WVR; also regarded as a method to simulate weightlessness). These results are basically in line with the results obtained on Swordtails from space flight.     

Cosmic ray detection on the Foton-M2 satellite by a track etch detector stack

In the frame of the European Space Agency (ESA) project called “Biology and Physics in Space”, the returning satellite, Foton-M2, carried an open-to-space exposure platform outside of the satellite body, called as BIOPAN-5, loaded with exo-biological experiments and facilities for radiation dosimetry (RADO). One of the RADO experiments was dedicated to the detection of the primary galactic cosmic rays (GCR) and secondary neutrons by a track etch detector stack. The daily absorbed dose (D) and dose equivalent (H) were calculated from the experimental LET spectra (LET > 10 keV/μm). Under a shielding of ∼2.8 g/cm² the averaged H was found to be 658 ± 8 μSv/d, with a quality factor (Q) of 6.2 ± 1.2. The LET spectra showed a local peak at ∼105 keV/μm suggesting that the majority of tracks were created by trapped protons as it has been predicted by calculations. The low LET dose of the cosmic radiation was determined by 4 TLD stacks, and the total dose was found to be 795 ± 14 μSv/d.     

Spectral analysis of time-integrated Konus–Wind GRBs: Implication on radiative mechanisms

We have analysed a sample of 328 time-integrated GRB prompt emission spectra taken via the Konus instrument on board the US GGS-Wind spacecraft between 2002 and 2004 using a couple of two-components models, Cut-off Power Law (CPL) + Power Law (PL) and blackbody (BB) + PL. The spectra show clear deviation from the Band function. The PL term is interpreted as the low energy tail of a nonthermal emission mechanism. The distributions of corresponding index β give values β < −2/3 consistent with synchrotron and synchrotron self-Compton mechanisms. The distribution of low energy index α associated with the CPL term shows clear discordance with synchrotron models for 31.4% of the analysed GRBs with values exceeding that for the line of death, α = −2/3. Then, a set of nonthermal radiation mechanisms producing harder slopes, i.e., α > −2/3, are presented and discussed. For the remaining majority (68.6%) of GRBs with CPL index α < −2/3, we show that optically thin synchrotron produced by a power law electron distribution of type, N(γ) ∼ γ^−p, γ₁ < γ < γ₂, for finite energy range (γ₂ ≠ ∞) is a likely emission mechanism with α ∼−(p + 1)/2 in the frequency range ν₁ ? ν ? ν₂ (where ν₂ = η²ν₁ with η = γ₂/γ₁), such that for p > 1/3, one gets α < −2/3. We also show that corresponding spectra in terms of F_ν and νF_ν functions are peaked around frequency ν₂ instead of ν₁, respectively for p < 1 and p < 3. Besides, thermal emission is examined taking a single Planck function for fitting the low energy range. It can be interpreted as an early emission from the GRB fireball photosphere with observed mean temperature, kT′ ∼ 16.8 keV. Furthermore, we have performed a statistical comparison between the CPL + PL and BB + PL models finding comparable χ²-values for an important fraction of GRBs, which makes it difficult to distinguish which model and specific radiation mechanism (possible thermal or nonthermal γ-ray emissions) are best suitable for describing the reported data. Therefore, additional information for those bursts, such as γ-ray polarization, would be highly desirable in future determinations of GRBs observational data.     

Dependence of the 27-day variation of cosmic rays on the global magnetic field of the Sun

We show that the higher range of the heliolongitudinal asymmetry of the solar wind speed in the positive polarity period (A > 0) than in the negative polarity period (A < 0) is one of the important reasons of the larger amplitudes of the 27-day variation of the galactic cosmic ray (GCR) intensity in the period of 1995–1997 (A > 0) than in 1985–1987 (A < 0). Subsequently, different ranges of the heliolongitudinal asymmetry of the solar wind speed jointly with equally important corresponding drift effect are general causes of the polarity dependence of the amplitudes of the 27-day variation of the GCR intensity. At the same time, we show that the polarity dependence is feeble for the last unusual minimum epoch of solar activity 2007–2009 (A < 0); the amplitude of the 27-day variation of the GCR intensity shows only a tendency of the polarity dependence. We present a three dimensional (3-D) model of the 27-day variation of GCR based on the Parker’s transport equation. In the 3-D model is implemented a longitudinal variation of the solar wind speed reproducing in situ measurements and corresponding divergence-free interplanetary magnetic field (IMF) derived from the Maxwell’s equations. We show that results of the proposed 3-D modeling of the 27-day variation of GCR intensity for different polarities of the solar magnetic cycle are in good agreement with the neutron monitors experimental data. To reach a compatibility of the theoretical modeling with observations for the last minimum epoch of solar activity 2007–2009 (A < 0) a parallel diffusion coefficient was increased by ∼40%.     

The LAGEOS satellites orbit and Yukawa-like interactions

LAGEOS II general relativity pericenter precession has been analysed in terms of the errors produced by the mismodelling of both the gravitational and non-gravitational perturbations acting on the satellite orbit. The accuracy in the pericenter determination may be considered as an upper-bound value for the estimate of the strength α of a possible new-long-range-interaction described by a Yukawa-like potential. In the present work we have focused on the constraints in α that can be obtained with the current best multi-satellites gravity field model EGM96 (α < 2.6 × 10⁻¹⁰) and also with the first promising models from the CHAMP (α < 1.8 × 10⁻¹⁰) and GRACE (α < 1.2 × 10⁻¹⁰) gravimetric missions. These results represent, potentially, an improvement of two or three orders-of-magnitude with respect to the best constraints obtained in the past with Earth–LAGEOS and Lunar–LAGEOS data (|α| < 10⁻⁵–10⁻⁸). The impact of the non-gravitational perturbations mismodelling in the final error budget has been determined together with the improvements obtainable in the constraint of the strength α with the proposed LARES satellite.     

Different mechanisms controlling interannual phytoplankton variation in the South China Sea and the western North Pacific subtropical gyre: A satellite study

A time series of remotely-sensed chlorophyll a (chl a) in 1997–2010 was evaluated to determine mechanisms of phytoplankton variation in recent decade in the South China Sea (SCS) and the western North Pacific subtropical gyre (WNPSG). Satellite-derived sea surface temperature (SST) and aerosol optical thickness (AOT) were used as proxies for vertical nutrient supply and atmospheric aerosol, respectively. Chl a in the WNPSG was not significantly correlated with SST (r = 0.18, p > 0.05), but was with AOT (r = 0.31, p < 0.05), indicating the chl a was influenced by atmospheric deposition. Chl a in the SCS was negatively correlated with SST (r = −0.60, p < 0.05) and was positively with AOT (r = 0.20, p < 0.05). The correlation between AOT and chl a in the SCS does not reflect a major contribution from atmospheric deposition to chl a; instead, the relationship resulted from concurrence of the peaks of AOT and wind speed, which drive water mixing and nutrient supply. Consequently, chl a in the SCS would be regulated primarily by the nutrient supply from deep waters. Because SST was controlled by the ENSO teleconnection in the SCS, the chl a was coupled with ENSO events. The present study demonstrated that interannual phytoplankton variation could be controlled by different factors even in neighboring oligotrophic regions.     

The effects of composting on the nutritional composition of fibrous bio-regenerative life support systems (BLSS) plant waste residues and its impact on the growth of Nile tilapia (Oreochromis niloticus)

Utilization of bio-regenerative life support systems (BLSS) plant waste residues as a nutritional source by Nile tilapia (Oreochromis niloticus) has proven problematic as a result of high concentrations of fibrous compounds in the plant waste residues. Nutritional improvement of plant waste residues by composting with the oyster mushroom (Pleurotus ostreatus), and the effects on growth and nutrient utilization of Nile tilapia fed such residues were evaluated. Five Nile tilapia (mean weight = 70.9 ± 3.1 g) were stocked in triplicate aquaria and fed one of two experimental diets, cowpea (CP) and composted cowpea (CCP), twice daily for a period of 8 weeks. Composting of cowpea residue resulted in reduced concentrations of nitrogen-free extract, hemi-cellulose and trypsin inhibitor activity, though trypsin inhibitor activity remained high. Composting did not reduce crude fiber, lignin, or cellulose concentrations in the diet. No significant differences (P < 0.05) were observed in weight gain, specific growth rate, survival rate, daily consumption, and food conversion ratio between tilapia fed CP and CCP. These results suggest that P. ostreatus is not a suitable candidate for culture in conjunction with the culture of Nile tilapia. Additional work is needed to determine what, if any, benefit can be obtained from incorporating composted residue as feed for Nile tilapia.     

Study of the 27-day variations of the galactic cosmic ray intensity and anisotropy

We demonstrate that the general features of the radial and azimuthal components of the anisotropy of galactic cosmic rays can be studied by the harmonic analysis method using data from an individual neutron monitor with cut off rigidity <5 GV. In particular, we study the characteristics of the 27-day (solar rotation period) variations of the galactic cosmic ray intensity and anisotropy, solar wind velocity, interplanetary magnetic field strength and sunspot number. The amplitudes of the 27-day variations of the galactic cosmic ray anisotropy are greater, and the phases more clearly established, in A > 0 polarity periods than in A < 0 polarity periods at times of minimum solar activity. The phases of the 27-day variations of the galactic cosmic rays intensity and anisotropy are opposite with respect to the similar changes of the solar wind velocity in A > 0 polarity periods. No significant dependence of the amplitude of the 27-day variation of the galactic cosmic ray anisotropy on the tilt angle of the heliospheric neutral sheet is found. Daily epicyclegrams obtained by Chree’s method show that the 27-day variations of the galactic cosmic ray anisotropy during A > 0 polarity periods follow elliptical paths with the major axes oriented approximately along the interplanetary magnetic field. The paths are more irregular during A < 0 polarity periods.     

X-ray redshifts with the International X-ray Observatory (IXO)

We explore the capabilities of the future space science mission IXO (International X-ray Observatory) for obtaining cosmological redshifts of distant Active Galactic Nuclei (AGNs) using the X-ray data only. We first find in which regions of the X-ray luminosity (L_X) versus redshift (z) plane the weak but ubiquitous Fe Kα narrow emission line can deliver an accurate redshift (δz < 5%) as a function of exposure time, using a CCD-based Wide Field Imager (IXO/WFI) as the one baselined for IXO. Down to a 2–10 keV X-ray flux of 10⁻¹⁴ erg cm⁻² s⁻¹ IXO/WFI exposures of 100 ks, 300 ks and 1 Ms will deliver 20%, 40% and 60% of the redshifts. This means that in a typical 18′ × 18′ IXO/WFI field of view, 4, 10 and 25 redshifts will be obtained for free from the X-ray data alone, spanning a wide range up to z ∼ 2–3 and fairly sampling the real distribution. Measuring redshifts of fainter sources will indeed need spectroscopy at other wavebands.     

Solar cycle and latitude dependence of high-beta suprathermal plasma conditions in interplanetary space between 1.3 and 5.4 AU

The analysis of energetic particles and magnetic field measurements from the Ulysses spacecraft has shown that in a series of events, the energy density contained in the suprathermal tail particle distribution is comparable to or larger than that of the magnetic field, creating conditions of high-beta plasma. In this work we analyze periods of high-beta suprathermal plasma occurrences (β_ep > 1) in interplanetary space, using the ratio (β_ep) of the energetic particle (20 keV to ∼5 MeV) and magnetic field energy densities from measurements covering the entire Ulysses mission lifetime (1990–2009) in order to reveal new or to reconfirm some recently defined interesting characteristics. The main key-results of the work are summarized as follows: (i) we verify that high-beta events are detected within well identified regions corresponding mainly to the vicinity of shock surfaces and magnetic structures, and associated with energetic particle intensity enhancements due to (a) reacceleration at shock-fronts and (b) unusually large magnetic field depressions. (ii) We define three considerable features for the high-beta events, concentrated on the next points: (a) there is an appreciable solar-activity influence on the high-beta events, during the maximum and middle solar-cycle phase, (b) the annual peak magnitude and the number of occurrences of high events are well correlated with the sunspot number, (c) the high-beta suprathermal plasma events present a spatial distribution in heliographic latitudes (HL) up to ∼±80°, and a specific important concentration on the low (−25° ? HL < −6°, 6° < HL ? 25°) and median (−45° ? HL < −25°, 25° < HL ? 45°) latitudes. We also reconfirm by a statistical analysis the results of Marhavilas and Sarris (2011), that the high-beta suprathermal plasma (β_ep > 1) events are characterized by a very large parameter β_ep (up to 1732.5), a great total duration (406 days) and a large percentage of the Ulysses-mission lifetime (which is equal to 6.34% of the total duration with usable measurements, and 11.3% of the duration in presence of suprathermal particles events).     

Advanced architectures for real-time Delay-Doppler Map GNSS-reflectometers: The GPS reflectometer instrument for PAU (griPAU)

In recent years Global Navigation Satellite System’s signals Reflectometry (GNSS-R) has stood as a potential powerful remote sensing technique to derive scientifically relevant geophysical parameters such as ocean altimetry, sea state or soil moisture. This has brought out the need of designing and implementing appropriate receivers in order to track and process this kind of signals in real-time to avoid the storage of huge volumes of raw data. This paper presents the architecture and performance of the Global Positioning System (GPS) Reflectometer Instrument for PAU (griPAU), a real-time high resolution Delay-Doppler Map reflectometer, operating at the GPS L1 frequency with the C/A codes. The griPAU instrument computes 24 × 32 complex points DDMs with configurable resolution (Δf_Dmin = 20 Hz, Δτ_min = 0.05 chips) and selectable coherent (minimum = 1 ms, maximum = 100 ms for correlation loss Δρ < 90%) and incoherent integration times (minimum of one coherent integration period and maximum not limited but typically <1 s). A high sensitivity (DDM peak relative error = 0.9% and DDM volume relative error = 0.03% @ T_i = 1 s) and stability (Δρ/Δt = −1 s⁻¹) have been achieved by means of advanced digital design techniques.     

Evaluation of upper body muscle activity during cardiopulmonary resuscitation performance in simulated microgravity

Performance of efficient single-person cardiopulmonary resuscitation (CPR) is vital to maintain cardiac and cerebral perfusion during the 2–4 min it takes for deployment of advanced life support during a space mission. The aim of the present study was to investigate potential differences in upper body muscle activity during CPR performance at terrestrial gravity (+1Gz) and in simulated microgravity (μG). Muscle activity of the triceps brachii, erector spinae, rectus abdominis and pectoralis major was measured via superficial electromyography in 20 healthy male volunteers. Four sets of 30 external chest compressions (ECCs) were performed on a mannequin. Microgravity was simulated using a body suspension device and harness; the Evetts–Russomano (ER) method was adopted for CPR performance in simulated microgravity. Heart rate and perceived exertion via Borg scores were also measured. While a significantly lower depth of ECCs was observed in simulated microgravity, compared with +1Gz, it was still within the target range of 40–50 mm. There was a 7.7% decrease of the mean (±SEM) ECC depth from 48 ± 0.3 mm at +1Gz, to 44.3 ± 0.5 mm during microgravity simulation (p < 0.001). No significant difference in number or rate of compressions was found between the two conditions. Heart rate displayed a significantly larger increase during CPR in simulated microgravity than at +1Gz, the former presenting a mean (±SEM) of 23.6 ± 2.91 bpm and the latter, 76.6 ± 3.8 bpm (p < 0.001). Borg scores were 70% higher post-microgravity compressions (17 ± 1) than post +1Gz compressions (10 ± 1) (p < 0.001). Intermuscular comparisons showed the triceps brachii to have significantly lower muscle activity than each of the other three tested muscles, in both +1Gz and microgravity. As shown by greater Borg scores and heart rate increases, CPR performance in simulated microgravity is more fatiguing than at +1Gz. Nevertheless, no significant difference in muscle activity between conditions was found, a result that is favourable for astronauts, given the inevitable muscular and cardiovascular deconditioning that occurs during space travel.     

Study of TEC changes during geomagnetic storms occurred near the crest of the equatorial ionospheric ionization anomaly in the Indian sector

GPS satellites data obtained at Bhopal (23.16° N, 77.36° E, geomagnetic latitude 14.23° N) India were analyzed to study the TEC changes during several geomagnetic storms (−300 nT < Dst < −50 nT) occurred in 2005–2007. We had segregated the storms according to the Dst value, i.e. moderate storms (−100 nT < Dst ? −50 nT), strong storms (−150 nT < Dst < −100 nT), and severe storms (Dst less than −150 nT). Total of 21 geomagnetic storms (10 moderate, 9 strong, 2 severe) are considered for the present study. Deviation in vertical total electron content (VTEC) during the main phase of the storm was found to be associated with the prompt penetration of electric field originated due to the under-shielding and over-shielding conditions for almost all geomagnetic storms discussed in this paper. For most of the storms VTEC shows the positive percentage deviation during the main phase while it shows positive as well as the negative deviation during the recovery phase of the storms. The −80% deviation in VTEC was found for geomagnetic storm occurred on July 17, 2005 and the negative trend continued for recovery phase of the storm. This was mainly due to the thermospheric composition changes by Joule heating effect at auroral latitudes that generate electric field disturbance at low latitudes. Traveling ionospheric disturbances (TIDs) were responsible for the formation of wave like nature in VTEC for the storms occurred on May 15, 2005, whereas it was not observed for storm occurred on August 24, 2005.     

QSO number counts to B < 24.5 from hubble space telescope WFPC2 images

We have conducted a survey for faint quasars to B < 24.5, z < 2.1 based on archival high-latitude Hubble Space Telescope Wide Field Planetary Camera 2 (WFPC2) images covering 0.04 deg² of the sky in three filters spanning 2300–9600 Å. Taking advantage of the superb 0.1″ resolution of WFPC2, we have developed an approach to select quasar candidates based on their morphology and position in multicolor space. We derive cumulative surface densities and compare them with our previous survey in the Groth–Westphal Strip and other faint quasar surveys that utilize very different selection approaches. In most cases the agreement with these surveys is good, however, we find approximately twice as many quasar candidates to B = 24.5 as the COMBO-17 survey. Although we are confident that our approach eliminates most contaminating stars and galaxies, spectroscopic follow-up will be required to determine the level of residual contamination and confirm or refute this excess.     

Probing the method of correcting Faraday rotation in vector magnetograms

By analyzing the vector magnetograms of Huairou Solar Observing Station (HSOS) taken at the line center (0.0 Å) and the line wing (−0.12 Å) of FeI λ5324.19 Å, we make an estimate of the measured errors in transversal azimuths (δ?) caused by Faraday rotation. Since many factors, such as the magnetic saturation and scattered light, can affect the measurement accuracy of the longitudinal magnetic field in the umbrae of sunspots, we limit our study in the region ∣B_z∣ < 800 G. The main mean azimuth rotations are about 4°, 6°, 7° and 9°, while ∣B_z∣ are in the ranges of 400–500 G, 500–600 G, 600–700 G and 700–800 G, respectively. Moreover, we find there is also an azimuth rotation of about 8° at the wavelength offset −0.12 Å of the line compared against a previous numerical simulation.     

Creation of model of quasi-trapped proton fluxes below Earth’s radiation belt

The object of investigation is the phenomenon of proton (from tens keV to several MeV) flux enhancement in near-equatorial region (L < 1.15) at altitude up to ∼1300 km (the storm-time equatorial belt). These fluxes are quite small but the problem of their origin is more interesting than the possible damage they can produce. The well known sources of these protons are radiation belt and ring current. The mechanism of transport is the charge-exchange on neutral hydrogen of exosphere and the charge-exchange on oxygen of upper atmosphere. Therefore this belt is something like the ring current projection to low altitudes. Using the large set of satellites data we obtain the average energy spectrum, the approximation of spectrum using kappa-function, the flux dependence on L, B geomagnetic parameters. On the basis of more than 30 years of experimental observations we made the empiric model that extends model of proton fluxes below 100 keV in the region of small L-values (L < 1.15). The model was realized as the package of programs integrated into COSRAD system available via Internet. The model can be used for revision of estimation of dose that low-orbital space devices obtain.     

Physiologic and metabolic responses of wheat seedlings to elevated and super-elevated carbon dioxide

The metabolic consequence of suboptimal (400 μmol mol⁻¹ or ppm), near-optimal (1500 ppm) and supra-optimal (10,000 ppm) atmospheric carbon dioxide concentrations [CO₂] was investigated in an attempt to reveal plausible underlying mechanisms for the differential physiological and developmental responses to increasing [CO₂]. Both non-targeted and targeted metabolite profiling by GC–MS and LC–MS were employed to examine primary and secondary metabolites in wheat (Triticum aestivum, cv Yocoro rojo) continuously exposed to these [CO₂] levels for 14, 21 and 28 days. Metabolite profile was altered by both [CO₂] and physiological age. In general, plants grown under high [CO₂] exhibited a metabolite profile characteristic of older plants under ambient CO₂. Elevated [CO₂] resulted in higher levels of phosphorylated sugar intermediates, though no clear trend in the content of reducing sugars was observed. Transient starch content was enhanced by increasing [CO₂] to a much greater extent at 10,000 ppm CO₂ than at 1500 ppm CO₂. The percentage increase of starch content resulting from CO₂ enrichment declined as plants develope. In contrast, elevated [CO₂] promoted the accumulation of secondary metabolites (flavonoids) progressively to a greater extent as plants became mature. Elevated [CO₂] to 1500 ppm induced a higher initial growth rate, while super-elevated [CO₂] appeared to negate such initial growth promotion. However, after 4 weeks, there was no difference in vegetative growth between 1500 and 10,000 ppm CO₂-grown plants, both elevated CO₂ levels resulted in an overall 25% increase in biomass over the control plants. More interestingly, elevated atmospheric [CO₂] reduced evapotranspiration rate (ET), but further increase to the supra-optimal level resulted in increased ET (a reversed trend), i.e. ET at 1500 ppm < ET at 10,000 ppm < ET at 400 ppm. The differential effect of elevated and super-elevated CO₂ on plants was further reflected in the nitrogen dynamics. These results provide the potential metabolic basis for the differential productivity and stomatal function of plants grown under elevated and super-elevated CO₂ levels.