Xenopus laevis embryos can establish their spatial bilateral symmetrical body pattern without gravity.

One assumes that gravity cooperates with the sperm in the establishment of bilateral symmetry in the embryo, particularly in species with yolky eggs. However, only experiments under genuine microgravity can prove this. May 2nd 1988 on the TEXUS-17 Sounding Rocket, eggs of Xenopus laevis became the first vertebrate eggs ever successfully fertilized in Space. Fertilization was done in fully automated hardware; the experiment was successfully repeated and extended in 1989. Here we report a "Space First" from the IML-1 Space Shuttle mission (January 1992): In similar hardware and under microgravity, artificially fertilized Xenopus eggs started embryonic development. Histological fixation was pre-programmed at the time gastrulation would occur on Earth and indeed, gastrulae were fixed. Thus after fertilization in near weightlessness Xenopus embryos do develop bilaterally symmetrically, very probably cued by the sperm alone.     

Experimental and numerical analysis of non-symmetric breakage of liquid columns in an axial gravitational field rotating around an eccentric axis

An experiment in microgravity conditions aboard the TEXUS-23 mission was performed to obtain the deformation up to breakage of a cylindrical liquid column in isorotation around an eccentric axis. In previous work, breakage rotation speed was predicted by a numerical method. This method was validated by comparison with analytical and experimental results. The non-symmetric breakage of the liquid column observed in the experiment, however, was not explained by the combined effect of rotation and eccentricity.     

Fertilization and development of eggs of the South African clawed toad, Xenopus laevis, on sounding rockets in space.

Egg rotation and centrifugation experiments strongly suggest a role for gravity in the determination of the spatial structure of amphibian embryos. Decisive experiments can only be made in Space. Eggs of Xenopus laevis, the South African clawed toad, were the first vertebrate eggs which were successfully fertilized on Sounding Rockets in Space. Unfixed, newly fertilized eggs survived reentry, and a reasonable number showed a seemingly normal gastrulation but died between gastrulation and neurulation. Only a few reached the larval stage, but these developed abnormally. In the future, we intend to test whether this abnormal morphogenesis is due to reentry perturbations, or due to a real microgravity effect, through perturbation of the reinitiation of meiosis and other processes, or started by later sperm penetration.     

Stability of liquid bridges subject to an eccentric rotation

A cylindrical liquid bridge supported between two circular-shaped disks in isorotation is considered. The effect of an offset between the rotation axis and the axis of the two supporting disks (eccentricity) on the stability of the static liquid bridge is investigated. A numerical method is used to find stable and unstable shapes and to determine the stability limit for different values of eccentricity. The calculated stability limits are compared with analytical results, recovering the same behavior. Numerical results have been also compared with the results of an experiment aboard TEXUS-23, recovering the stability limit and the equilibrium shapes.     

In vivo fertilization and development in microgravity using pleurodele ("ZEUS" project).

The objectives of this experiment are to perform natural fertilization and to achieve embryonic development in microgravity. Pleurodeles waltl, an urodele amphibian, is considered by CNES and NASA to be suitable experimental material for achieving in vivo fertilization in space. Previously inseminated females can be embarked in the Frog Environmental Unit (FEU) developed by NASA. Laying of eggs will be provoked by hormonal stimulation in flight and development will be followed. Various technical problems have been resolved in laboratory experiments and during parabolic flights : the time of hormone stimulation after insemination, choice of hormone guaranteeing [correction of guarenteing] 95% success, other factors conditioning [correction of conditionning] the laying, experimental procedures to study developmental kinetics at phenotypic levels, and selection of cellular and molecular markers of development.     

Cytoskeleton and gravity at work in the establishment of dorso-ventral polarity in the egg of Xenopus laevis

The establishment of polarities during early embryogenesis is essential for normal development. Amphibian eggs are appropriate models for studies on embryonic pattern formation. The animal-vegetal axis of the axially symmetrical amphibian egg originates during oogenesis and foreshadows the main body axis of the embryo. The dorso-ventral polarity is epigenetically established before first cleavage. Recent experiments strongly suggest that in the monospermic eggs of the anuran Xenopus laevis both the cytoskeleton and gravity act in the determination of the dorso-ventral polarity. In order to test the role of gravity in this process, eggs will be fertilized under microgravity conditions during the SL-D1 flight in 1985. In a fully automatic experiment container eggs will be kept under well-defined conditions and artificially fertilized as soon as microgravity is reached; eggs and embryos at different stages will then be fixed for later examination. Back on earth the material will be analysed and we will know whether fertilization under microgravity conditions is possible. If so, the relation of the dorso-ventral axis to the former sperm entry point will be determined on the whole embryos; in addition eggs and embryos will be analysed cytologically.     

Subcellular components of the amphibian egg: insights provided by gravitational studies.

Most cytoplasmic regions of fertilized amphibian eggs move with respect to the gravity vector in experimentally gravity oriented eggs. The pattern and extent of this movement varies among different batches of eggs. This variation in apparent cytoplasmic viscosity (or, conversely, cytoplasmic mobility) can be correlated with variations in subsequent morphogenesis of experimental, gravitationally manipulated eggs. Therefore, the proper interpretation of gravity experiments with amphibian eggs requires that one understand the subcellular basis for this variation on cytoplasmic mobility. Variation in the packing of the major cytoplasmic organelle, the yolk platelets, or the organization and amount of cytoskeletal components may explain the variation in cytoplasmic mobility. Evidence is presented that the variation in yolk volume density (fraction of total cytoplasmic volume occupied by yolk platelets) does not account for the variation in cytoplasmic mobility in Xenopus laevis eggs. Experimental evidence from cold-shocked inverted eggs indicates that microtubules may be involved in determining cytoplasmic mobility. However, quantitative evidence that the microtubule levels and state of the microtubules (polymerized vs. non-polymerized) in the whole Xenopus laevis egg does not correlate directly with cytoplasmic mobility is presented. The apparent conflict these data represent regarding the role of the cytoskeleton in determining cytoplasmic mobility is discussed.     

探空火箭图像压缩算法研究

结合探空火箭图像系统需求, 提出了一种基于小波变换编码, 即基于MSPIHT的自适应差分算术编码(MSPIHT Adaptive Differential Arithmetic Coding for image, MADAC)的图像压缩算法. 该算法对经过量化编码之后的数据 进行熵编码. 实验结果表明, MADAC算法具备探空火箭图像系统所要求的分辨 率、帧速率及压缩率可调的功能, 且易于硬件实现.      

Early results based on the stratospheric channels of TOVS on the TIROS-N series of operational satellites

TOVS (TIROS Operational Vertical Sounder) comprises the three radiometers HIRS-2 (High resolution Infra Red Sounder Mod 2), MSU (Microwave Sounding Unit) and SSU (Stratospheric Sounding Unit) mounted on the TIROS-N series of operational spacecraft. Data from these sounders have been available since October 1978 and the series should continue to beyond 1985. The derivation and assessment of stratospheric geopotential height fields from these sounders, with particular reference to the SSU, are described. Measurements of stratospheric diurnal temperature effects and plots of zonally meaned brightness temperatures over a 450 day period are also presented.     

空间重粒子击中卤虫卵的定位技术及其生物效应

为了能够估价空间重粒子对单个生物细胞的放射生物学效应,我们设计、制成了卤虫卵-核乳胶夹层式生物包.经搭载我国“8885”卫星飞行数天后回收.用图象分析仪研究了空间重离子在核乳胶上留下的径迹及其分布;并实验观察了卤虫卵的前期发育情况.看到:随星飞行过的卤虫卵与地面对照组相比,其发育能力明显降低,表现为孵化率低且发育时间推迟.实验表明:生物样品板-核乳胶影象定位技术能清楚而比较准确地给出卤虫卵(或其他生物细胞)受重离子作用的信息,是重离子生物效应及其作用机理研究的可行实验手段及分析方法之一.     

实践十号卫星有效载荷胶体材料箱地基实验

胶体材料箱是装载于实践十号上的重要载荷,用于空间胶体自组装的实验研究.地基实验阐明了常重力下的蒸发驱动胶体自组装机制.围绕胶体材料箱开展地基实验研究,制备了一种亲/疏水限位基片,分析了蒸发过程中受限液滴接触角的变化规律.通过同步显微观察法研究受限胶体液滴内部粒子的沉积行为,发现粒子沉积图案的形成过程由三种动力学行为控制.另外,通过落塔装置模拟短时微重力环境,分析重力瞬变引起限位基片上液滴的振荡过程,揭示了振荡过程中两个不同阶段的振荡特性.地基实验结果为在轨实验工况确定以及空间与地面实验对比提供了数据支撑,这对箱体工程参数设定以及空间实验条件匹配等具有重要意义.      

A direct approach to the study of the effect of gravity on axis formation in birds.

A system has been developed to enable the normal development of aborted very early uterine avian embryos, outside the female's uterus. The shell-less aborted egg was put into a foster shell of a sister egg, previously laid by the same female. The empty space between the shell and aborted egg was filled with artificial uterine fluid. The reconstructed eggs were incubated at 42 degrees C for 30 hours in a vertical position. The atmosphere contained a high concentration of CO2 (8-10%). At the termination of the 30 h the eggs were transferred to incubation at 37 degrees C in normal atmospheric conditions. Normal development has been recorded for a certain percentage of eggs incubated up to 12 days. In other cases abnormalities, arrested development or development of extraembryonic membranes only, without a sign of an embryonic axis, have been observed. The three important conclusions from the above experiments were: 1. It is possible to develop a closed, self-contained system, disconnected from the female's body, that would support the development of early uterine embryos. 2. The incidence of embryo-less extraembryonic membranes in such a system, is correlated with the degree of detachment of the "yolk" from the outer envelopes. 3. Such a system can be further developed into an experiment suited for microgravity conditions which will be an alternative to an experiment with live birds. The experiment will be aimed at testing the importance of gravity in changing the radially symmetrical avian blastoderm into a bilaterally symmetrical blastoderm.     

微重力与电磁场对凝固过程的影响

欧洲空间局两项材料领域课题MICAST和CETSOL均涉及微重力科学相关研究.通过等轴晶柱状晶转变（CET）分析了微重力环境对材料凝固过程的影响;介绍了一项在地面施加行波电磁场的标准（Benchmark）凝固实验,其温度场演化及微观凝固组织和浓度偏析均展示了磁控受迫对流对金属材料凝固过程的显著作用.      

力约束管材自由胀形试验研究与材料性能测试

相较于单向拉伸试验,通过管材胀形试验(TBT,Tube Bulging Test)获得的材料性能参数能够更准确地反映材料在高压流体作用下的塑性成形性能,不同的管端边界将会严重影响管材胀形试验的测试结果.针对国际上现有试验方法和设备存在的不足,研制出了一套约束边界清晰、加载精确的管材自由胀形试验系统.在管材测试过程中,基于位移随动力主动加载的控制策略和比例伺服油缸,实现实时的轴向力、轴向位移和内压力的精确加载.端部约束的测试管材通过特殊设计的工装保证了其轴向自由滑动.实时内压力和胀形管材顶点处材料的壁厚和胀形高度信息通过超高压压力传感器、超声测厚仪和磁致伸缩位移传感器采集,进而基于Swift材料本构模型和采集到的数据拟合出材料应力应变曲线和材料性能参数.试验结果表明,管材两端侧推力与内压力对管材内腔端面的作用力和管材轴向自由对称收缩的平衡条件始终处于动态稳定中,试验设备能够准确获得实时胀形高度、顶点厚度、轴向收缩长度和内压力的信息,能够为材料性能测试和工艺设计提供可信的材料参数.      

天舟一号货运飞船空间实验装置蒸发相变地面实验

以天舟一号货运飞船为依托,开展空间蒸发相变传热规律的科学实验研究,探索重力对蒸发传热传质过程的影响规律.设计了一套地面蒸发实验平台,以蒸发相变液体FC-72为研究对象,通过红外热像仪测温、热流量计、差分热电偶等手段,观测FC-72液层在不同台面温度、注液量等情况下的相变界面变化、蒸发表面特性、流体物性及Marangoni对流涡胞的变化等,获取其蒸发两相流体的液层温度差、表面温度场、热流量值、蒸发速率和涡胞结构等.实验结果表明:在其他条件不变的情况下,FC-72液层与蒸发台面的温差越高,其蒸发速率越快;注液量越大,蒸发速率也越大;在蒸发过程中出现了浮力对流涡胞和Marangoni对流涡胞.此外,通过地面蒸发实验可以确定空间科学实验选用的实验介质和材料,进而优化确定空间科学实验的工况、参数及流程等,部分地面实验结果也将直接成为天地对比实验的科学成果.      

AstroNewt: early development of newt in space.

AstroNewt experiment explores the effects of earth gravity on the early development of Japanese red-bellied newt, Cynops pyrrhogaster. Since female newts keep spermatophore in cloaca, fertilized eggs could be obtained without mating. Fertilization of newt's egg occurs just prior to spawning, so that gonadotrophic cues applied to females in orbit leads to laying eggs fertilized just in space. A property of newt being kept in hibernation at low temperature may be of great help for the space experiment carried out with much limited resources. A general outline of the AstroNewt project is shown here in addition to some technical advances for the development of the project. Experimental schemes of two space experiments (IML-2 in summer 1994 and unmanned SFU at the beginning of 1995) are also shown.     

Validation of the stratospheric sounding unit

The Stratospheric Sounding Unit (SSU) is part of the TOVS (TIROS Operational Vertical Sounder) on NOAA operational meteorological satellites. SSU measurements can be validated by comparison with temperature measurements from colocated rocket sondes. Systematic differences are found which vary with rocket station and sonde and are a function of height. However, these measurements are not adequate to define the performance of individual SSUs to a precision which would allow the observations from different SSUs to be combined in the study of diurnal and semidiurnal tides and of long term trends in stratospheric temperature. Instead this is achieved by detailed radiometric and spectroscopic investigation of each individual SSU, both prior to launch and during its operational life. Using the techniques descirbed, it is demonstrated that measurements from different SSUs can be combined with a relative error of less than 0.2K in equivalent brightness temperature.     

Digisonde observations of TIDs with frequency and angular sounding technique

A technique for studying ionospheric wavelike phenomena, primarily AGW/TID events, is developed based on the solution of the problem of radio wave propagation in ionospheric plasma disturbed by wavelike processes. A perfectly reflecting surface model is used for representing TIDs propagating at ionospheric heights. This technique is a generalization of the Frequency-and-Angular Sounding (FAS) method developed earlier for oblique TID diagnostics using transmitters of opportunity. Trial measurements were made in November 2003 with two DPS-4 systems at Millstone Hill Observatory, providing experimental validation of the developed method by comparing the results of disturbance diagnostics to those simultaneously obtained with the original (oblique) FAS method. The TID parameters recovered during the November 2003 campaign suggest that the observed disturbances predominately propagated equatorward which likely indicates their sources to be in the auroral region. The equatorward propagating AGW/TIDs are typical for disturbed geomagnetic conditions which were observed during the campaign. Implementation of the generalized FAS technique in the DPS sounder allowed development of a dedicated data acquisition system for ionospheric disturbance diagnostics. Routine measurements with the developed technique using the existing world-wide network of Digisondes (GIRO) will make it possible to conduct large-scale studies of the AGW/TID phenomena.