空间重粒子击中卤虫卵的定位技术及其生物效应

为了能够估价空间重粒子对单个生物细胞的放射生物学效应,我们设计、制成了卤虫卵-核乳胶夹层式生物包.经搭载我国“8885”卫星飞行数天后回收.用图象分析仪研究了空间重离子在核乳胶上留下的径迹及其分布;并实验观察了卤虫卵的前期发育情况.看到:随星飞行过的卤虫卵与地面对照组相比,其发育能力明显降低,表现为孵化率低且发育时间推迟.实验表明:生物样品板-核乳胶影象定位技术能清楚而比较准确地给出卤虫卵(或其他生物细胞)受重离子作用的信息,是重离子生物效应及其作用机理研究的可行实验手段及分析方法之一.

LOCATION TECHNIQUE AND BIOLOGICAL EFFECT FOR ARTEMIA SALINA EGG HITED BY HEAVY IONS DURING SPACEFLIGHT

We designed and manufactured a biostack, monolayers of Artemia Salina eggs sandwiched between nuclear emulsions, to investigate the biological effect of single heavy ions to individual cells. It was exposed on board "8885" Chinese Satellite during eight-day flight and then after it returned to the ground it was collected. The distribution of tracks left by space heavy ions on emulsion was studed by means of image analysis instrument. And the development of Artemia Salina eggs was observed. Comparing with control group on ground, we found that the group on board have lower hatching rate and delayed developing time. Out of 90 experimental eggs only 8 eggs showed development at different levels. Among them 4 hatching eggs were not hit by the heavy ion and among the other 4 emerging eggs there were 3 eggs hit. Under the same condition hatching rate of control group on ground reached at almost 60%. These phenomena are obviously related to the action of heavy ions in space.From the results of the test we reached the following conclusion: the biosample plate-nuclear emulsion shadow location technique can give information of Artemia Salina eggs hit by heavy ions clearly and exactly. That is one of thte experimental and analysing methods useful to investigate biological effects and mechanism on heavy ion action.