Spacelab Life Sciences flight experiments: an integrated approach to the study of cardiovascular deconditioning and orthostatic hypotension

The microgravity environment of spaceflight produces rapid cardiovascular changes which are adaptive and appropriate in that setting, but are associated with significant deconditioning and orthostatic hypotension on return to Earth's gravity. The rapidity with which these space flight induced changes appear and disappear provides an ideal model for studying the underlying pathophysiological mechanisms of deconditioning and orthostatic hypotension, regardless of etiology. Since significant deconditioning is seen after flights of very short duration, muscle atrophy due to inactivity plays, at most, a small role. These changes in circulatory control associated with cephalad fluid shifts, rather than inactivity per se, are probably more important factors. In order to test this hypothesis in a systematic way, a multidisciplinary approach which defines and integrates inputs and responses from a wide variety of circulatory sub-systems is required. The cardiovascular experiments selected for Spacelab Life Sciences flights 1 and 2 provide such an approach. Both human and animal models will be utilized. Pre- and post-flight characterization of the payload crew includes determination of maximal exercise capacity (bicycle ergometry), orthostatic tolerance (lower body negative pressure), alpha and beta adrenergic sensitivity (isoproterenol and phenylephrine infusions), baroreflex sensitivity (ECG-gated, stepwise changes in carotid artery transmural pressure with a pneumatic neck collar), and responses to a 24 h period of 5 deg head-down tilt. Measurements of cardiac output (CO2 and C2H2 rebreathing), cardiac chamber dimensions (phased-array 2-dimensional echocardiography), direct central venous pressure, leg volume (Thornton sock), limb blood flow and venous compliance (occlusion plethysmography), blood and plasma volumes, renal plasma flow and glomerular filtration rates, and various hormonal levels including catecholamines and atrial natriuretic factor will also be obtained. The central venous catheter will be inserted immediately pre-launch and monitored with heart rate and blood pressure in-flight until cardiac output, respiratory gas exchange and quantitative 2D echocardiography measurements can be performed. In-flight hemodynamic measurements will be repeated at rest and during submaximal exercise daily and also during maximal exercise midway through the flight to document the timecourse and extent of cardiovascular changes in the payload crew. Parallel studies are planned for the animals. In addition to measurements of right atrial and aortic pressures and cardiac output, a dorsal micro-circulatory chamber will allow determinations of changes in capillary and venular architecture and function in six of the rats. The techniques and findings from many of the SLS-1 and 2 supporting studies have already yielded significant information about circulatory regulation in patients with both hypo- and hypertension. The flight experiments themselves will provide new data to test the validity of both animal and human models currently used for simulating the fluid shifts of a micro-gravity environment. The development of effective countermeasures, not only for short and long duration space travellers, but also for Earth-bound medical patients can then be physiologically based on experimental data rather than anecdote.     

Lower body negative pressure (LBNP) as a countermeasure for long term spaceflight

The article presents the current status of lower body negative pressure (LBNP) as a countermeasure for preventing orthostatic intolerance after space flight or bed rest. Devices discussed include the Chibis vacuum suit, the Anthrorack device, a collapsible device, and an inflatable device. Two bed rest studies examined the effect of LBNP and exercise on orthostatic tolerance; plasma volume; vasopressin, plasma renin activity, and catecholamines; and side effects.     

The cost benefits of the Spacelab system to scientific investigations and space applications

In the past, one of the major problems in performing scientific investigations in space has been the high cost of developing, integrating, and transporting scientific experiments into space. The limited resources of unmanned spacecraft, coupled with the requirements for completely automated operations, was another factor contributing to the high costs of scientific research in space. In previous space missions after developing, integrating and transporting costly experiments into space and obtaining successful data, the experiment facility and spacecraft have been lost forever, because they could not be returned to earth. The objective of this paper is to present how the utilization of the Spacelab System will result in cost benefits to the scientific community, and significantly reduce the cost of space operations from previous space programs.The following approach was used to quantify the cost benefits of using the Spacelab System to greatly reduce the operational costs of scientific research in space. An analysis was made of the series of activities required to combine individual scientific experiments into an integrated payload that is compatible with the Space Transportation System (STS). These activities, including Shuttle and Spacelab integration, communications and data processing, launch support requirements, and flight operations were analyzed to indicate how this new space system, when compared with previous space systems, will reduce the cost of space research. It will be shown that utilization of the Spacelab modular design, standard payload interfaces, optional Mission Dependent Equipment (MDE), and standard services, such as the Experiment Computer Operating System (ECOS), allow the user many more services than previous programs, at significantly lower costs. In addition, the missions will also be analyzed to relate their cost benefit contributions to space scientific research.The analytical tools that are being developed at MSFC in the form of computer programs that can rapidly analyze experiment to Spacelab interfaces will be discussed to show how these tools allow the Spacelab integrator to economically establish the payload compatibility of a Spacelab mission.The information used in this paper has been assimilated from the actual experience gained in integrating over 50 highly complex, scientific experiments that will fly on the Spacelab first and second missions. In addition, this paper described the work being done at the Marshall Space Flight Center (MSFC) to define the analytical integration tools and techniques required to economically and efficiently integrate a wide variety of Spacelab payloads and missions. The conclusions reached in this study are based on the actual experience gained at MSFC in its roles of Spacelab integration and mission managers for the first three Spacelab missions. The results of this paper will clearly show that the cost benefits of the Spacelab system will greatly reduce the costs and increase the opportunities for scientific investigation from space.     

Status and efficacy of countermeasures to physiological deconditioning from space flight

The recent biomedical investigations conducted on the Space Shuttle and Spacelab have provided a wealth of biomedical information, including the ability to test the efficacy of proposed countermeasures. This achievement was made possible by the ability to conduct mechanistic and control-interventive studies simultaneously with a large number of individuals over a relatively brief period and to compare these data with results obtained from the Skylab missions. Comparisons between short- and long-duration results were limited to establishing trends or extrapolating from short-duration missions. To date, we have evaluated several protocols involving the lower-body negative pressure (LBNP) device, the bicycle-ergometer, the treadmill and preparations for body-fluid replenishment. In many instances, the traditional means of applying these protocols were not sufficient to protect against space-related deconditioning. This paper will review current countermeasures and compare their efficacy to that of existing protocols. Results from in-flight and ground-based experiments will be presented to illuminate the recommended protocols and procedures.     

Role of the astronaut in operating the Advanced Fluid Physics Module

The role of man in space is investigated in the operation of the Advanced Fluid Physics Module (AFPM), a scientific instrument dedicated to fluid physics research in a microgravity environment and flown on the Spacelab D2 mission. The astronaut involvement is addressed by applying the criteria of the THURIS study, conducted by NASA for the optimization of future manned space flights. Outcomes of the THURIS study are first summarized. The AFPM characteristics and interfaces are briefly presented. The five experiments performed on board Spacelab D2 are introduced and the involvement of the astronaut is described. Finally, THURIS criteria are applied to an AFPM experiment scenario. Results show that, of all the activities involved in the AFPM nominal operation, two thirds are related to hardware manipulation and to procedure following, while the last third uses the unique astronaut intellectual capabilities, making his presence in orbit mandatory for successful experiment completion.     

Cost effectiveness of Spacelab experiments

Spacelab permits investigation in new seicntific disciplines like material processing, life sciences, chemistry, etc. The large mass and volume capabilities of Spacelab offer better possibilities for some areas of traditional space sciences like infrared astronomy, multi-spectral solar observations and large instruments for astronomical observations.Since free-flyers will require normally a new spacecraft development for each mission, the reusability of space qualified components and experiments will be a significant cost reduction factor over a long period. In the early phase of Spacelab utilisation, however, the scaling factor introduced by Spacelab utilisation, however, the scaling factor introduced by Spacelab results in higher payload development costs than originally appreciated.The costs of Spacelab utilisation are computed and compared with those of conventional free-flying satellites. The mission implementation costs and experiment development costs are shown for both cases. The Spacelab mission implementation costs are subdivided into NASA charges for the Standard Shuttle Mission, NASA charges to fly and operate Spacelab, the European costs of Spacelab payload integration and experiment development costs. In order to evaluate and compare mission implementation costs, the simple parameters are adopted of the cost per kg of experiments and the data collection-transmission capability of Shuttle/Spacelab and ESRO/ESA satellites. The mission implementation costs turn out to be very favourable for Spacelab. The experiment development costs, which are not included in the mission implementation costs, are compared for several free flyers with the corresponding development costs for several experiments of the first Spacelab payload. The comparison shows that the cost per kg of Spacelab experiment development is about five times less than of satellite experiments.     

Using Spacelab as a precursor of science operations for the Space Station

For more than 15 years, Spacelab, has provided a laboratory in space for an international array of experiments, facilities, and experimenters. In addition to continuing this important work, Spacelab is now serving as a crucial stepping-stone to the improved science, improved operations, and rapid access to space that will characterize International Space Station. In the Space Station era, science operations will depend primarily on distributed/remote operations that will allow investigators to direct science activities from their universities, facilities, or home bases. Spacelab missions are a crucial part of preparing for these activities, having been used to test, prove, and refine remote operations over several missions. The knowledge gained from preparing these Missions is also playing a crucial role in reducing the time required to put an experiment into orbit, from revolutionizing the processes involved to testing the hardware needed for these more advanced operations. This paper discusses the role of the Spacelab program and the NASA Marshall Space Flight Center- (MSFC-) managed missions in developing and refining remote operations, new hardware and facilities for use on Space Station, and procedures that dramatically reduce preparation time for flight.     

Hematological and immunological changes during space flight.

This paper gives a summary of the principal hematological and immunological changes observed in crews after space flight. Reduction of red blood cell mass (2-21%) and of hemoglobin mass (12-33%) is generally observed after the US and Soviet space missions. The changes are accompanied with a loss of plasma volume (4-16%). Erythrocyte and hemoglobin concentrations in the blood remain constant, suggesting that the changes are driven by a feed-back mechanism. Immunological changes consist mainly of reduced T-lymphocyte reactivity. The results of the 96-day and 140-day Salyut-6 missions suggest that the adaptation of the immune system to spaceflight occurs in two stages: the first takes place during the first 2-3 months in space, the second follows and consists of further weakening of the immune response. Our experiments with human lymphocytes in vitro indicate that high-g enhance, whereas low-g depress lymphocyte activity. Finally, our investigations to be performed on Spacelab are described.     

European materials science investigations and associated experimental equipment within the Spacelab demonstration missions

This paper reviews shortly the results obtained by a preliminary call for experiment proposals for future Spacelab flights issued by the European Space Agency in April 1978. The results of this call indicate clearly the trend towards experiments performing studies on the state and the evolution of fluid media. The instrumentation used are mainly multipurpose instruments (furnaces, process chambers) already under development for the first Spacelab flight and new equipment currently under study.     

Low-gravity environment in Spacelab

This paper presents residual and system-generated accelerations with results from g-jitter spectral measurements in the Spacelab Engineering Model. An overview (classification, brief discussion, and assessment of magnitudes) of the various constituents of the perturbative acceleration field inside the Spacelab Module is presented, both steady and fluctuating components being considered. Results of local g-jitter spectral measurements taken in the Spacelab Engineering Model (EM-1)/Long Module Configuration are presented for frequencies from less than 1 to 200 Hz. The measured results for the system-generated perturbative accelerations exhibit, in the time domain, amplitudes of the order of 10(-3) g (peak value 3.6 x 10(-3) g). Spectral values of 4 x 10(-4) g are obtained in the frequency range up to 100 Hz; up to 10 Hz, however, the spectral values remain about an order of magnitude smaller, and also between 100 and 200 Hz the perturbation level is significantly lower than below 100 Hz. Measured results from simulated crew activities show, in the time domain, a peak amplitude of 2.6 x 10(-2)g, the spectral values being 6 x 10(-3)g below 100 Hz and 1 x 10(-3)g below 10 Hz for typical perturbances.     

Dosimetric measurements in manned missions

Detector packages consisting of thermoluminescence detectors (TLDs), nuclear emulsions and plastic nuclear track detectors were exposed in different sections of the MIR space station, inside the Spacelab during the IML1 mission, and inside Spacelab module and tunnel during the D2 mission. This report concentrates on total dose measurements with TLDs during these mission. The results are discussed and compared to results of former missions and to calculations. Finally, dose equivalents and mean quality factors for each mission are presented which are derived from the TLD results and results obtained from the other detector systems. Dose equivalents range between 200 μSvd⁻¹ and 700 μSvd⁻¹.     

Reflex contributions to the assessment of the vertical

On the ground the vertical directions "up" and "down" have significance in relation to the strategy for avoiding collision of the skull with the planet. Voluntary acts to this end may be based on the experienced result of reflexly generated motor commands. Relevant receptors lie in the otolith organs of the labyrinth, but the head is seldom steady in waking life. A revised scheme of labyrinth reflexes on the limbs--"downhill limbs extend"--replaces the classical scheme of Magnus. Interactions with neck reflexes according to this scheme serve to stabilize the trunk. In an orbiting spacecraft the pattern of afferent signals from the labyrinth differs from that on the ground, and predictions based on the new scheme are to be tested in the project "Operation Push-Pull" proposed for ESRO's Spacelab. Other activities of the Council of Europe's Working Party on Aerospace Physiology and Medicine are briefly described.     

Electrostatic and acoustic instrumentation for material science processing in space

The use of electrostatic forces in the design of a positioning system and acoustic forces in the implementation of a mixing system for material science experiments on Spacelab are described. The electrostatic positioning of samples is described with special reference to its advantages and disadvantages with regard to other positioning methods. The design of such a positioner is described including the considerations relating to the processing of both high and low vapour pressure materials in a positioner compatible with both the isothermal heating facility (IHF) and the mirror heating facility (MHF) of Spacelab under microgravity (10^?4–10^?3 g) conditions. The application of acoustic and ultrasonic forces to the problem of sample mixing in material science experiments is explained. The design of a mixer compatible with existing furnace hardware for Spacelab and capable of effectively mixing samples at temperatures up to 1200°C is described. Tests of the mixer show that a 15 μm displacement adequate for good mixing can be achieved with a d.c. power input of 23 W and a conversion efficiency of 70%. Tests on alumina particles and carbon fibres in various alloy matrices show that complete wetting can be achieved.     

Microgravity measurement assembly (MMA) for spacelab module missions

The microgravity measurement assembly (MMA) is a precision measurement facility for ground and on-orbit disturbance accelerations on board Spacelab, being currently under development by MBB/ERNO under DFVLR contract. MMA is using a new generation of micromechanical acceleration detectors developed by CSEM under ESTEC contract. Small dimensions of the triaxial sensor packages allow for installation very close to scientific experiments; mass is significantly reduced compared to conventional systems. Six or more of these mini-sensor packages are installed at the most g-sensitive experiments of Spacelab Module Missions. Acceleration and housekeeping data are processed in real time by a dedicated microcomputer and transmitted to the ground. Thus, for the first time, synchronized and comparable precision acceleration data are available in real time on ground for on-line judgement of the microgravity environment desired for experiment success, offering the possibility, for example of experiment repetition in case of excessive g-disturbances. Furthermore, MMA allows for immediate feedback to the crew concerning the microgravity effects of their dynamic behavior, with the aim of crew training towards lower disturbances. An additional mobile sensor package can be installed at vibration sources, e.g. pumps, centrifuges etc. or any arbitrary location inside the Spacelab Module. An impact hammer can be used together with MMA in order to measure in-flight structural transfer functions. The MMA on-board system and ground station and its planned utilization for the German Spacelab Mission D-2 is described.     

Bioinstrumentation for evaluation of workload in payload specialists: results of ASSESS II

ASSESS II (Airborne Science/Spacelab Experiments System Simulation) was a cooperative NASA-ESA project which consisted of a detailed simulation of Spacelab operations using the NASA Ames Research Center CV-990 aircraft laboratory. The Medical Experiment reported on in this paper was part of the complex payload consisting of 11 different experiments. Its general purpose was to develop a technology, possibly flown on board of Spacelab, and enabling the assessment of workload through evaluating changes of circadian rhythmicity, sleep disturbances and episodical or cumulative stress. As parameters the following variables were measured: Rectal temperature, ECG, sleep-EEG and -EOG, the urinary excretion of hormones and electrolytes. The results revealed evidence that a Spacelab environment, as simulated in ASSESS II, will lead to internal dissociation of circadian rhythms, to sleep disturbances and to highly stressful working conditions. Altogether these effects will impose considerable workload upon Payload Specialists. It is suggested that an intensive pre-mission system simulation will reduce these impairments to a reasonable degree. The bioinstrumentation applied in this experiment proved to be a practical and reliable tool in assessing the objectives of the study.     

Does mental arithmetic before head up tilt have an effect on the orthostatic cardiovascular and hormonal responses?

Passive head up tilt (HUT) and mental arithmetic (MA) are commonly used for providing mental and orthostatic challenges, respectively. In animal experiments, even a single exposure to a stressor has been shown to modify the response to subsequent stress stimulus. We investigated whether MA applied before HUT elicits synergistic responses in orthostatic heart rate (HR), cardiac output (CO), heart rate variability and arterial blood pressure. The 15 healthy young males were subjected to two randomized protocols: (a) HUT and (b) HUT preceded by MA, with sessions randomized and ≥2 weeks apart. Beat to beat continuous hemodynamic variables were measured and saliva samples taken for hormonal assay. HUT alone increased HR from 59±7 (baseline) to 80±10 bpm (mean±SD) and mean blood pressure (MBP) from 88±10 to 91±14 mmHg. HUT results after MA were not different from those with HUT alone. The activity of alpha amylase showed differences during the experiments irrespective of the protocols. We conclude that mental challenge does not affect orthostatic cardiovascular responses when applied before; the timing of mental loading seems to be critical if it is intended to alter cardiovascular responses to upright standing.     

The metric camera experiment on the first spacelab flight

In the first Spacelab Mission which will take place in Sept. Oct. 1983 a Metric Camera will be flown as part of the Earth observation payload. The camera will be a modified high quality Aerial Survey Camera.The hardware development is finished and the instrument is already integrated into Spacelab.The application of Metric Cameras in Space, an area which is neglected up to now, can effectively contribute to an improved cartographic coverage of the Earth. The Metric Camera Experiment is a first step to fill this gap which can be realized by utilizing the extended capacities of the Space Transportation System.The paper outlines the scientific objectives of the experiment, describes in detail the camera system and deals with the operation and control philosophy during the mission.     

Space radiation dosimetry

In this report dosimetric measurements are presented which were performed during the missions Spacelab 1, D 1, Biocosmos 8 and Biocosmos 9. Detector packages consisting of plastic nuclear track detectors, nuclear emulsions and thermoluminescence dosimeters were exposed at different locations inside and outside spacecrafts behind more or less well defined shielding thicknesses. These detector systems which supplement each other in their registration characteristic allow to record all biological relevant portions of the radiation field separately. Dose equivalents for the astronauts have been calculated from the measurements using the quality factors as defined in the old and the new recommendations of the International Commission on Radiation Protection (ICRP).     

Prospective life-science payloads

A viable spacelab programme is based on the thesis that biomedical specialists require a quantifiable, and possibly mechanistic, understanding of the significant changes observed in crew, in and after manned space flights. Only then can prophylaxis or atraumatic reversal be achieved (with potentially an added use to ameliorate qualitatively similar disease aspects on Earth). This approach could justify national funding to promote lead-up ground-based research as well as research and development for special equipment, of which the "spin-off" into clinical practice could well precede its first use in Spacelab. The requirement for "applied expediency" arises from the watershed met early in the evolution of a life-sciences programme. Initially, the facility of space flight provoked numerous valid experiments designed to test for, or quantitate, gravity-dependent mechanisms and their interaction with other agents, radiation, vibration, or absence of triggers for rhythmic patterns. In parallel, measurable parameters of man's function in space were being monitored, primarily to promote survival by remedial action when available. Monitoring data were then developed to find a critical mechanism feasible to testing. Often the rationale for such tests and experiments was that "man was there" and could, moreover, attend to several biological experiments in space! The watershed appeared when man in a Spacelab was shown as a hazard to the instrumentation, cleanliness, accuracy, thermal control, weight limits, etc. essential to the other disciplines. Other than the life sciences only the technological requirements of materials processing required a manned spacelab! So, life scientists have needed to rethink their payloads, and their constrictions, to plan for compatible load sharing. A composite of proposed biomedical projects related to apparently unanswered etiology of observed changes in returning astronauts will be used to illustrate the evolution of and possible answers to sample problems. The principles outlined, their moderation by expediency (with the untouched upon need for the enthusiastic involvement of biomedical potential in space projects) should remain our guidelines. This is in spite of the expected obsolescence of these specific projects within the next decade.