Survival of Bacillus pumilus spores for a prolonged period of time in real space conditions

To prevent forward contamination and maintain the scientific integrity of future life-detection missions, it is important to characterize and attempt to eliminate terrestrial microorganisms associated with exploratory spacecraft and landing vehicles. Among the organisms isolated from spacecraft-associated surfaces, spores of Bacillus pumilus SAFR-032 exhibited unusually high resistance to decontamination techniques such as UV radiation and peroxide treatment. Subsequently, B. pumilus SAFR-032 was flown to the International Space Station (ISS) and exposed to a variety of space conditions via the European Technology Exposure Facility (EuTEF). After 18 months of exposure in the EXPOSE facility of the European Space Agency (ESA) on EuTEF under dark space conditions, SAFR-032 spores showed 10-40% survivability, whereas a survival rate of 85-100% was observed when these spores were kept aboard the ISS under dark simulated martian atmospheric conditions. In contrast, when UV (>110?nm) was applied on SAFR-032 spores for the same time period and under the same conditions used in EXPOSE, a ～7-log reduction in viability was observed. A parallel experiment was conducted on Earth with identical samples under simulated space conditions. Spores exposed to ground simulations showed less of a reduction in viability when compared with the "real space" exposed spores (～3-log reduction in viability for "UV-Mars," and ～4-log reduction in viability for "UV-Space"). A comparative proteomics analysis indicated that proteins conferring resistant traits (superoxide dismutase) were present in higher concentration in space-exposed spores when compared to controls. Also, the first-generation cells and spores derived from space-exposed samples exhibited elevated UVC resistance when compared with their ground control counterparts. The data generated are important for calculating the probability and mechanisms of microbial survival in space conditions and assessing microbial contaminants as risks for forward contamination and in situ life detection.     

The O/OREOS mission: first science data from the Space Environment Survivability of Living Organisms (SESLO) payload

We report the first telemetered spaceflight science results from the orbiting Space Environment Survivability of Living Organisms (SESLO) experiment, executed by one of the two 10?cm cube-format payloads aboard the 5.5?kg Organism/Organic Exposure to Orbital Stresses (O/OREOS) free-flying nanosatellite. The O/OREOS spacecraft was launched successfully to a 72° inclination, 650?km Earth orbit on 19 November 2010. This satellite provides access to the radiation environment of space in relatively weak regions of Earth's protective magnetosphere as it passes close to the north and south magnetic poles; the total dose rate is about 15 times that in the orbit of the International Space Station. The SESLO experiment measures the long-term survival, germination, and growth responses, including metabolic activity, of Bacillus subtilis spores exposed to the microgravity, ionizing radiation, and heavy-ion bombardment of its high-inclination orbit. Six microwells containing wild-type (168) and six more containing radiation-sensitive mutant (WN1087) strains of dried B. subtilis spores were rehydrated with nutrient medium after 14 days in space to allow the spores to germinate and grow. Similarly, the same distribution of organisms in a different set of microwells was rehydrated with nutrient medium after 97 days in space. The nutrient medium included the redox dye Alamar blue, which changes color in response to cellular metabolic activity. Three-color transmitted intensity measurements of all microwells were telemetered to Earth within days of each of the 48?h growth experiments. We report here on the evaluation and interpretation of these spaceflight data in comparison to delayed-synchronous laboratory ground control experiments.     

The BOSS and BIOMEX space experiments on the EXPOSE-R2 mission: Endurance of the desert cyanobacterium Chroococcidiopsis under simulated space vacuum,Martian atmosphere,UVC radiation and temperature extremes.

The proposed space experiments BOSS (Biofilm Organisms Surfing Space) and BIOMEX (BIOlogy and Mars experiment) will take place on the space exposure facility EXPOSE-R2 on the International Space Station (ISS), which is set to be launched in 2014. In BOSS the hypothesis to be tested is that microorganisms grown as biofilms, hence embedded in self-produced extracellular polymeric substances, are more tolerant to space and Martian conditions compared to their planktonic counterparts. Various microbial biofilms have been developed including those obtained from the cyanobacterium Chroococcidiopsis isolated from hot and cold deserts. The prime objective of BIOMEX is to evaluate to what extent biomolecules are resistant to, and can maintain their stability under, space and Mars-like conditions; therefore a variety of pigments and cell components are under investigation to establish a biosignature data base; e.g. a Raman spectral library to be used for extraterrestrial life biosignatures. The secondary objective of BIOMEX is to investigate the endurance of extremophiles, focusing on their interactions with Lunar and Martian mineral analogues. Ground-based studies are currently being carried out in the framework of EVTs (Experiment Verification Tests) by exposing selected organisms to space and Martian simulations. Results on a desert strain of Chroococcidiopsis obtained from the first set of EVT, e.g. space vacuum, Mars atmosphere, UVC radiation, temperature cycles and extremes, suggested that dried biofilms exhibited an enhanced survival compared to planktonic lifestyle. Moreover the protection provided by a Martian mineral analogue (S-MRS) to the sub-cellular integrities of Chroococcidiopsis against UVC radiation supports the endurance of this cyanobacterium under extraterrestrial conditions and its relevance in the development of life detection strategies.     

Species and temperature exchange in the atmosphere of “BION-M” spacecraft

On going flights of Foton satellites allow to carry out research in the following domains: effect of space flight and outer space factors such as microgravity, artificial gravity and space radiation on physical processes and biological organisms. Experts from many Russian and foreign scientific institutions participated in the research. Over a period of time from 1973 to 1997 there were launched 11 BION satellites designed by the Central Specialized Design Bureau for carrying out fundamental and applied research in the field of space biology, medicine, radio physics and radiobiology with participation of specialists from the foreign countries.The goal of the present investigation was in developing a numerical simulator aimed at determining gas concentration and temperature fields established inside the scientific module of the spacecraft “Bion-M” and to perform optimization studies, which could meet strong requirements for air quality and temperature range allowable for operation of different biological experiments.     

Survival of rock-colonizing organisms after 1.5 years in outer space

Cryptoendolithic microbial communities and epilithic lichens have been considered as appropriate candidates for the scenario of lithopanspermia, which proposes a natural interplanetary exchange of organisms by means of rocks that have been impact ejected from their planet of origin. So far, the hardiness of these terrestrial organisms in the severe and hostile conditions of space has not been tested over extended periods of time. A first long-term (1.5 years) exposure experiment in space was performed with a variety of rock-colonizing eukaryotic organisms at the International Space Station on board the European EXPOSE-E facility. Organisms were selected that are especially adapted to cope with the environmental extremes of their natural habitats. It was found that some-but not all-of those most robust microbial communities from extremely hostile regions on Earth are also partially resistant to the even more hostile environment of outer space, including high vacuum, temperature fluctuation, the full spectrum of extraterrestrial solar electromagnetic radiation, and cosmic ionizing radiation. Although the reported experimental period of 1.5 years in space is not comparable with the time spans of thousands or millions of years believed to be required for lithopanspermia, our data provide first evidence of the differential hardiness of cryptoendolithic communities in space.     

Mechanics of Zero Gravity and Gravitationally Sensitive Systems (First Issue)

This issue of the journal contains papers with the results of studying gravitationally sensitive systems and processes under conditions of microgravity aboard the Mirorbital complex. This is one of the most difficult field of space research whose difficulties are due to both complexity of the object of investigations (since the class of gravitationally sensitive systems and processes is fairly wide, and some of them are not sufficiently studied even under terrestrial conditions) and necessity of using expensive instrumentation and carrying out long-term experiments. However, studying the new mechanical state of weightlessness is inevitable in space exploration. In addition, in some cases it can provide for a new knowledge about the fundamental laws of nature. By virtue of the above reasons, the experiments on microgravity are well presented in the research program onboard the International Space Station.There is a long tradition in this field of research in Russia, and the experience of Russian scientists (a part of which is presented in the papers of this issue) allows them to pass on to the next, better technically equipped, stage of investigations in cooperation with the scientists from other countries.This issue is prepared by an initiative of the subsection Mechanics of zero gravity and gravitationally sensitive systemsof the Coordination Scientific and Technical Council (CSTC) of the Russian Agency for Aviation and Space Flights (Rosaviakosmos).     

Frequency fluctuations of coherent signals from spacecraft observed during dual-frequency radio sounding of the circumsolar plasma in 2004–2008

We have performed spectral processing of the data of experiments on radio sounding of circumsolar plasma by coherent S- and X-band signals from the spacecraft Ulysses, Mars Express, Rosetta, and Venus Express carried out from 1991 to 2009. The experiments were realized in the mode of coherent response, when a signal stabilized by the hydrogen standard is transmitted from the ground station to a spacecraft, received by the onboard systems, and retransmitted to the Earth with conserved coherence. Thus, the signal sounding the coronal plasma passes twice through the medium: on the propagation path ground station — spacecraft and on the same path in the opposite direction. The spectra of frequency fluctuations in both the bands are obtained and, using them, the radial dependences of fluctuation intensities are found, which can be approximated by a power law. It is shown that the ratio of intensities of frequency fluctuations in the S- and X-bands is comparable with the theoretical value and characterizes the degree of correlation of irregularities of the electron density along the propagation path ground station — spacecraft and back. Analysis of the correlation of frequency fluctuations on the two paths allows one to get a lower estimate of the outer scale of the circumsolar plasma turbulence. For heliocentric distances R = 10 solar radii (R _S ) the outer scale is larger than 0.25R _S .     

Survival of spores of the UV-resistant Bacillus subtilis strain MW01 after exposure to low-earth orbit and simulated martian conditions: data from the space experiment ADAPT on EXPOSE-E

In the space experiment "Molecular adaptation strategies of microorganisms to different space and planetary UV climate conditions" (ADAPT), bacterial endospores of the highly UV-resistant Bacillus subtilis strain MW01 were exposed to low-Earth orbit (LEO) and simulated martian surface conditions for 559 days on board the European Space Agency's exposure facility EXPOSE-E, mounted outside the International Space Station. The survival of B. subtilis MW01 spores from both assays (LEO and simulated martian conditions) was determined by a colony-formation assay after retrieval. It was clearly shown that solar extraterrestrial UV radiation (λ≥110?nm) as well as the martian UV spectrum (λ≥200?nm) was the most deleterious factor applied; in some samples only a few spore survivors were recovered from B. subtilis MW01 spores exposed in monolayers. However, if shielded from solar irradiation, about 8% of MW01 spores survived in LEO conditions, and 100% survived in simulated martian conditions, compared to the laboratory controls. The results demonstrate the effect of shielding against the high inactivation potential of extraterrestrial solar UV radiation, which limits the chances of survival of even the highly UV-resistant strain of B. subtilis MW01 in the harsh environments of outer space and the martian surface.     

Examining the effects of anti-space weaponization arguments in the media: Some experimental findings from Canada

This study examines how political activists are framing the space weaponization debate in Canada and whether their arguments can influence public attitudes and perceptions about the issue. Eighty university students from two undergraduate courses were recruited as participants in a quasi-experiment. One class (n = 38) was exposed to the documentary Masters of Space, an episode of the Canadian Broadcasting Corporation's program The Nature of Things, and another class (n = 42) served as the control group. Pre-test and post-test questionnaires were used to measure the effects of viewing anti-weaponization arguments in the media, while also controlling for the influence of prior beliefs and background characteristics of participants. Results suggest that visually depicting the use of satellite technology in society can convince viewers that satellites are important to their way of life, but not necessarily to the defense of North America. Framing missile defense as a ‘space weapon in disguise’ also seemed to raise opposition to Canada's participation in continental missile defense. The findings, meanwhile, indicate that viewers respond strongly to the issue of space debris and that mobilizing support for joint military space projects may best be achieved by emphasizing the usefulness of these projects for locating and tracking such debris. In the end, media exposure seemed to help legitimize anti-weaponization arguments based more on rational self-interests than on idealistic beliefs. These findings can have implications for the way space policies are communicated to the public.     

Study of radiation conditions onboard the International space station by means of the Liulin-5 dosimeter

For estimating radiation risk in space flights it is necessary to determine radiation dose obtained by critical organs of a human body. For this purpose the experiments with human body models are carried out onboard spacecraft. These models represent phantoms equipped with passive and active radiation detectors which measure dose distributions at places of location of critical organs. The dosimetric Liulin-5 telescope is manufactured with using three silicon detectors for studying radiation conditions in the spherical tissue-equivalent phantom on the Russian segment of the International space station (ISS). The purpose of the experiment with Liulin-5 instrument is to study dynamics of the dose rate and particle flux in the phantom, as well as variations of radiation conditions on the ISS over long time intervals depending on a phase of the solar activity cycle, orbital parameters, and presence of solar energetic particles. The Liulin-5 dosimeter measures simultaneously the dose rate and fluxes of charged particles at three depths in the radial channel of the phantom, as well as the linear energy transfer. The paper presents the results of measurements of dose rate and particle fluxes caused by various radiation field components on the ISS during the period from June 2007 till December 2009.     

Microdisturbances on the International Space Station during dynamic operations

The results of analysis of microdisturbances on the International Space Station (ISS) at performing various dynamic operations are presented. Docking of transfer manned and cargo vehicles Progress and Soyuz to various docking modules of the ISS, docking of the Space Shuttle Discovery, the ISS orbit correction and, also, disturbances at “EVA” (Extra Vehicular Activity) operations during astronauts working on the external ISS surface are considered. The results of measuring microaccelerations by sensors of both Russian and American segments are analyzed.     

Alexandre Ananoff (1910–1992): 30 years to promote astronautics before Sputnik

2012 marks the 20th anniversary of Alexandre Ananoff's death. Born in 1910 in Tbilisi, Georgia, the Russian–French space expert and enthusiast Alexandre Ananoff is known for being the organiser of the first International Astronautical Congress at La Sorbonne University, Paris, in September–October 1950, as well as being the author of the famous book L’Astronautique (1950) and for advising fellow author Hergé for his book adventures of Tintin on the Moon. The purpose of our paper is to present his complete works promoting space, from his first public presentations after discovering the works of Tsiolkovsky in the late 20s to his analysis of the first Sputnik launch in the popular French magazine Paris Match in 1957.A. Ananoff was a real ambassador for astronautics and a pioneer in space education for the general public—probably the first one in France. He built a unique collection of books and novels about space travel (donated before his death to the French Air and Space Museum of Paris-Le Bourget), wrote dozens of articles, gave numerous lectures and corresponded with most of the space pioneers around the world. However, he was criticised for being an autodidact and was very disappointed by the lack of consideration accorded him within his own country. During the 60s, he progressively stopped his space activities to turn his attention to becoming a specialist of 18th century French painters. He published his Memoirs of an Astronaut in 1978 and gave his last lecture at IAC in September 1979. He died in Paris on 25 December 1992, aged 82. His last wish was that his ashes could be sent on the Moon, an end accomplishment for all his efforts.     

Harnessing functional food strategies for the health challenges of space travel—Fermented soy for astronaut nutrition

Astronauts face numerous health challenges during long-duration space missions, including diminished immunity, bone loss and increased risk of radiation-induced carcinogenesis. Changes in the intestinal flora of astronauts may contribute to these problems. Soy-based fermented food products could provide a nutritional strategy to help alleviate these challenges by incorporating beneficial lactic acid bacteria, while reaping the benefits of soy isoflavones. We carried out strain selection for the development of soy ferments, selecting strains of lactic acid bacteria showing the most effective growth and fermentation ability in soy milk (Streptococcus thermophilus ST5, Bifidobacterium longum R0175 and Lactobacillus helveticus R0052). Immunomodulatory bioactivity of selected ferments was assessed using an in vitro challenge system with human intestinal epithelial and macrophage cell lines, and selected ferments show the ability to down-regulate production of the pro-inflammatory cytokine interleukin-8 following challenge with tumour necrosis factor-alpha. The impact of fermentation on vitamin B1 and B6 levels and on isoflavone biotransformation to agluconic forms was also assessed, with strain variation-dependent biotransformation ability detected. Overall this suggests that probiotic bacteria can be successfully utilized to develop soy-based fermented products targeted against health problems associated with long-term space travel.     

Near space as a sui generis zone: A tri-layer approach of delimitation

The lack of a legal boundary between air space and outer space has not given rise to significant difficulties in the determination of applicable law with respect to traditional flight craft – aircraft and space objects, due to their separated sphere of activities. But the advent of new flight craft that are capable of operating in the intermediate “near space”, i.e. Near Space Vehicles, would render a clarification of their applicable law and the legal status of the zone requisite. For the purpose of balancing the right of exploration and use of near space and the security interest of subjacent States, this short note proposes a tri-layer approach of delimitation by which near space is established as a sui generis zone reserved exclusively for peaceful purposes, while the space below the upper operative limit of aircraft and that above the lower operative limit of space craft are air space and outer space respectively.     

Resistance of bacterial endospores to outer space for planetary protection purposes--experiment PROTECT of the EXPOSE-E mission

Spore-forming bacteria are of particular concern in the context of planetary protection because their tough endospores may withstand certain sterilization procedures as well as the harsh environments of outer space or planetary surfaces. To test their hardiness on a hypothetical mission to Mars, spores of Bacillus subtilis 168 and Bacillus pumilus SAFR-032 were exposed for 1.5 years to selected parameters of space in the experiment PROTECT during the EXPOSE-E mission on board the International Space Station. Mounted as dry layers on spacecraft-qualified aluminum coupons, the "trip to Mars" spores experienced space vacuum, cosmic and extraterrestrial solar radiation, and temperature fluctuations, whereas the "stay on Mars" spores were subjected to a simulated martian environment that included atmospheric pressure and composition, and UV and cosmic radiation. The survival of spores from both assays was determined after retrieval. It was clearly shown that solar extraterrestrial UV radiation (λ≥110?nm) as well as the martian UV spectrum (λ≥200?nm) was the most deleterious factor applied; in some samples only a few survivors were recovered from spores exposed in monolayers. Spores in multilayers survived better by several orders of magnitude. All other environmental parameters encountered by the "trip to Mars" or "stay on Mars" spores did little harm to the spores, which showed about 50% survival or more. The data demonstrate the high chance of survival of spores on a Mars mission, if protected against solar irradiation. These results will have implications for planetary protection considerations.     

Usage of pre-flight data in short rendezvous mission of Soyuz-TMA spacecrafts

The paper describes the reduction of the vehicle autonomous flight duration before docking to the ISS. The Russian Soyuz-TMA spacecraft dock to the ISS two days after launch. Due to the limited volume inside Soyuz-TMA the reduction of time until docking to the ISS is very important, since the long stay of the cosmonauts in the limited volume adds to the strain of the space flight. In the previous papers of the authors it was shown that the existing capabilities of Soyuz-TMA, the ISS and the ground control loop make it possible to transfer to the five-orbit rendezvous profile. However, the analysis of the cosmonauts' schedule on the launch day shows that its duration is at the allowable limit and that is why it is necessary to find a way to further reduce the flight duration of Soyuz-TMA before docking to less than five orbits. In a traditional rendezvous profile, the calculation of rendezvous burns begins only after determination of the actual vehicle insertion orbit. The paper describes an approach in which the first two rendezvous burns are performed as soon as the spacecraft reaches the reference orbit and the values of the burns are calculated prior to the launch based on the pre-flight data for the nominal insertion. This approach decreases the duration of the rendezvous by one orbit. The demonstration flight of a Progress vehicle using the proposed profile was implemented on August 1, 2012 and completely confirmed the correctness of the imbedded principles. The paper considers the possible improvements of the proposed approach and recovery from the contingencies.     

Integrated Studies of Electric Propulsion Engines during Flights in the Earth’s Ionosphere

Fifty years ago, on October 1, 1966, the first Yantar satellite laboratory with a gas plasma–ion electric propulsion was launched into orbit as part of the Yantar Soviet space program. In 1966–1971, the program launched a total of four laboratories with thrusters operating on argon, nitrogen, and air with jet velocities of 40, 120, and 140 km/s, respectively. These space experiments were the first to demonstrate the long-term stable operation of these thrusters, which exceed chemical rocket engines in specific impulse by an order of magnitude and provide effective jet charge compensation, under the conditions of a real flight at altitudes of 100–400 km. In this article, we have analyzed the potential modern applications of the scientific results obtained by the Yantar space program for the development of air-breathing electric propulsion that ensure the longterm operation of spacecraft in very low orbits.     

Influence of simulated and natural space environment factors on dielectric properties of epoxyamine polymers and polymer-based composite materials

The variations of dielectric properties of epoxyamine polymers and polymer-based composite materials during ground-based tests imitating the influence of space environment factors and under the conditions of long-term active experiments using the ERE instrumentation onboard the Mir station are compared. The influence of space environment factors is shown to result in both reversible and irreversible changes of dielectric properties. The former are related to temperature effects and effects of charged particles. The latter are related to the removal of low-molecular components from the composition of binders in a vacuum, and to increasing density of polymeric lattice under an effect of thermal cycling and various types of radiations. It is established that the influence of ultra-violet radiation reveals itself, first of all, in changing temperature of samples, while the influence of irradiation by a flux of electrons is reduced to charge accumulation and discharges.Translated from Kosmicheskie Issledovaniya, Vol. 43, No. 1, 2005, pp. 27–35.Original Russian Text Copyright © 2005 by Babaevsky, Kozlov, Churilo, Slagoda.