A thermodynamic analysis of microbial growth experiments

The common thread of energy release suggests that diverse microbial metabolic processes can be compared through thermodynamic analyses. The resulting energy and power requirements can provide quantitative constraints on habitability. Because previous thermodynamic analyses have focused on the minimum amount of energy needed for the growth of a microorganism or community, the focus of this study is to gain a fuller understanding of the microbial response to highly habitable conditions. This communication summarizes the results of a thermodynamic analysis of the energy and power consumed by microorganisms in experiments that were designed to optimize growth. Reports of microbial growth experiments taken from the literature were combined with speciation and standard state calculations to assess the overall Gibbs energy change during the experiments. Results show that similar numbers of cells (10(9) to 10(10) ) were produced in these experiments regardless of the duration of log phase growth (from <2 to >200 hours) or the total Gibbs energy change [from 1.3-29.6 kJ (mol electrons transferred)(1)]. As a result, optimal growth conditions appear to produce between 10(10) and 10(14) cells per watt of power consumed.     

Quantitative habitability

A framework is proposed for a quantitative approach to studying habitability. Considerations of environmental supply and organismal demand of energy lead to the conclusions that power units are most appropriate and that the units for habitability become watts per organism. Extreme and plush environments are revealed to be on a habitability continuum, and extreme environments can be quantified as those where power supply only barely exceeds demand. Strategies for laboratory and field experiments are outlined that would quantify power supplies, power demands, and habitability. An example involving a comparison of various metabolisms pursued by halophiles is shown to be well on the way to a quantitative habitability analysis.     

Formate as an energy source for microbial metabolism in chemosynthetic zones of hydrothermal ecosystems

Formate, a simple organic acid known to support chemotrophic hyperthermophiles, is found in hot springs of varying temperature and pH. However, it is not yet known how metabolic strategies that use formate could contribute to primary productivity in hydrothermal ecosystems. In an effort to provide a quantitative framework for assessing the role of formate metabolism, concentration data for dissolved formate and many other solutes in samples from Yellowstone hot springs were used, together with data for coexisting gas compositions, to evaluate the overall Gibbs energy for many reactions involving formate oxidation or reduction. The result is the first rigorous thermodynamic assessment of reactions involving formate oxidation to bicarbonate and reduction to methane coupled with various forms of iron, nitrogen, sulfur, hydrogen, and oxygen for hydrothermal ecosystems. We conclude that there are a limited number of reactions that can yield energy through formate reduction, in contrast to numerous formate oxidation reactions that can yield abundant energy for chemosynthetic microorganisms. Because the energy yields are so high, these results challenge the notion that hydrogen is the primary energy source of chemosynthetic microbes in hydrothermal ecosystems.