Influence of ionospheric perturbations in GPS time and frequency transfer

The stability of GPS time and frequency transfer is limited by the fact that GPS signals travel through the ionosphere. In high precision geodetic time transfer (i.e. based on precise modeling of code and carrier phase GPS data), the so-called ionosphere-free combination of the code and carrier phase measurements made on the two frequencies is used to remove the first-order ionospheric effect. In this paper, we investigate the impact of residual second- and third-order ionospheric effects on geodetic time transfer solutions i.e. remote atomic clock comparisons based on GPS measurements, using the ATOMIUM software developed at the Royal Observatory of Belgium (ROB). The impact of third-order ionospheric effects was shown to be negligible, while for second-order effects, the tests performed on different time links and at different epochs show a small impact of the order of some picoseconds, on a quiet day, and up to more than 10 picoseconds in case of high ionospheric activity. The geomagnetic storm of the 30th October 2003 is used to illustrate how space weather products are relevant to understand perturbations in geodetic time and frequency transfer.     

Characterization of Bacilli isolated from the confined environments of the Antarctic Concordia station and the International Space Station

Bacillus and related genera comprise opportunist and pathogen species that can threaten the health of a crew in confined stations required for long-term missions. In this study, 43 Bacilli from confined environments, that is, the Antarctic Concordia station and the International Space Station, were characterized in terms of virulence and plasmid exchange potentials. No specific virulence feature, such as the production of toxins or unusual antibiotic resistance, was detected. Most of the strains exhibited small or large plasmids, or both, some of which were related to the replicons of the Bacillus anthracis pXO1 and pXO2 virulence elements. One conjugative element, the capacity to mobilize and retromobilize small plasmids, was detected in a Bacillus cereus sensu lato isolate. Six out of 25 tested strains acquired foreign DNA by conjugation. Extremophilic bacteria were identified and exhibited the ability to grow at high pH and salt concentrations or at low temperatures. Finally, the clonal dispersion of an opportunist isolate was demonstrated in the Concordia station. Taken together, these results suggest that the virulence potential of the Bacillus isolates in confined environments tends to be low but genetic transfers could contribute to its capacity to spread.