Influence of clinorotation and fettering stress on tail regeneration of Triturus vulgaris (Urodela).

Tail-amputated adult Triturus vulgaris, fettered in cuvettes of a fast-rotating clinostat were exposed to simulated weightlessness (60 rpm; equiv. to 10(-3)-10(-4) g), during a 14-day period. To feed and clean the animals rotation was stopped once a day for approx. 10 min. To test the influence of the fettering stress, a second series of animals was kept separately under normal earth conditions without rotation. A further control series was kept in a dark container without any handicap. While tail regeneration of the rotated animals was markedly accelerated, the fettered-only animals showed a considerably less marked acceleration effect. At the end of the 14-day period, all regenerates were reamputated together with an additional 5 mm of the tail stump. Although this second level of amputation was distant from the first, the regenerative growth rate of the rotated series was accelerated 123% in contrast to both the control and the fettered-only series. Our results demonstrate that the growth acceleration is induced by clinorotation. Fettering stress has no comparable influence. The growth promoting effect is not limited to the regenerating area.     

Study on the Anisotropy of Meniscus in the Growing Sapphire

This study is aimed at predicting the relationship between the meniscus and the quality of the sapphire crystals produced by the micro-pulling and shoulder at cooled center (SAPMIC) technique. As with different orientations, the shapes of the meniscus vary, so an investigation into the anisotropy of the meniscus shapes is very important for the final quality of the sapphire crystal. An effective model to describe meniscus shapes and their formation process has been presented. The model has been applied to a sapphire crystal of 200 mm diameter in order to check its reliability. The results show that the model proves to be useful for forecasting the final shapes of the sap-phire crystal made by the SAPMIC technique.     

Spectral characteristics of electron fluxes at L < 2 under the Radiation Belts

The paper presents the analysis of experimental data on electron fluxes with energies 10 keV–10 MeV. Data were obtained during 1978–2005 years in different space experiments (COSMOS-900, MIR Space Station, ACTIVE, SAMPEX, CORONAS-I, CORONAS-F, NOAA POES-17, TATYANA and others). Two areas of electron flux enhancements are studied in the paper: the near-equatorial (L < 1.2) zone and the middle-latitude (1.2 < L < 1.9) zone. It is shown that electron flux enhancements are regularly registered at L < 2 and the observed formations have some typical features. Electron peaks at L < 1.2 appear sporadically while peaks at 1.2 < L < 1.9 are observed regularly. The approximations of spectra by several functions including kappa-function are presented.     

Urodelean amphibians in studies on microgravity: effects upon organ and tissue regeneration.

Results obtained from nine experiments performed onboard Russian biosatellites have shown that microgravity promotes tissue regeneration in the newt, Pleurodeles waltl. The effect has been reproduced in all flights and on a clinostat as well for eye tissues (lens and retina), limbs and tail. The effect was demonstrated in 1.5- to 2-fold increase in cell proliferation in the early stages of regeneration in space flight. Animals "flown" intact and operated after flight regenerated faster than control ones and showed long-lasting micro-"g" effect. The most recent experiment flew aboard the Bion-11 biosatellite. This test was performed for study on microgravity effect on neural retina regeneration after optic nerve lesioning in the newt. Obtained results confirmed our previous information about intensification of regenerative processes in detached neural retina in urodela exposed to simulated weightlessness (Grigoryan et al., 1998). In particular, we found the increase and activation of cell populations participating in neural retina restoration and maintenance of retinal structure. Our findings suggest that promoting effect of microgravity upon regeneration could be influenced by several factors, largely influenced by a response of the whole organism to changed gravity vector. We hypothesized the synthesis of the specific range of stress proteins induced by micro-"g" and their regulative role in cell proliferation. Such a hypothesis for the existence of "altered gravity stress proteins" is discussed.     

The electron formations under the radiation belts at L-shells 1.2–1.9

We continue to analyze the distribution of electron fluxes with energy 30–500 keV under the radiation belts at low and middle latitudes (L = 1.2–1.9) using experimental data obtained onboard ACTIVE satellite. Special attention is given to altitudinal distribution of electron fluxes and detailed analysis of these electron formations. We observe three main regions of electron flux registration that seem to exist constantly under the radiation belts. These regions are: magneto-conjugated to SAA region (in the north hemisphere), local zone of low intense electron flux accumulation to the west of SAA, and extensive region in the north hemisphere to the east. The analysis of experimental data obtained from ACTIVE satellite (orbit height 500–2500 km) shows that electron fluxes are registered in the wide altitude range up to 1100 km. It is shown that these formations have complicated initial structure with two regions of flux maximums: at L = 1.3 and L = 1.6–1.8. We compare particle data with low frequency (LF) data (ARIEL-4 satellite) and high frequency (HF) data (CORONAS-I satellite). Also we discuss the possible mechanisms of the appearance of these formations under the radiation belts.     

Regeneration of organs and tissues in lower vertebrates during and after space flight.

In this paper most important data obtained in studies on the effect of space flight conditions on regeneration in the adult newt are summarized. We demonstrate a phenomenon of synchronization of limb and lens regeneration and increase in its rate during and after space flight. We also describe a peculiarities of cell proliferation in lens, limb and tail regenerates and of the process of minced muscle regeneration.     

Near-Equatorial Electrons as Measured onboard the MirSpace Station

Some results of studying the electrons with energies of tens to hundreds of keV at the low and near- equatorial geomagnetic latitudes by using the instruments Sprut-V and Ryabina-2 onboard the Mirspace station in 1991 are presented. It is found that at L< 1.2 the enhanced electron fluxes are sporadically detected, being localized within three longitudinal intervals, 180° W–0°–15° E, 90°– 120° E, and 160° E–180°–135° W. The most intense electron fluxes are observed at the lower edge of the near-equatorial boundary of the inner radiation belt on longitudes of the South Atlantic Anomaly between 14 and 20 h MLT. The occurrence of electron bursts does not depend on the geomagnetic disturbance level. A hardening of the electron spectra is observed near the geomagnetic equator. At L< 1.1, the more energetic particles are located closer to the geomagnetic equator. The results are compared with the data on the low-frequency waves and fields at low and near-equatorial latitudes obtained by the Ariel-4and San Marco Dsatellites, as well as by the spacecraft and ground-based observations of the thunderstorm global distribution. The thunderstorms are considered as a possible source of electron production near the geomagnetic equator.     

Signs of Müller cell gliotic response found in the retina of newts exposed to real and simulated microgravity

The effects of real and simulated microgravity on the eye tissue regeneration of newts were investigated. For the first time changes in Müller glial cells in the retina of eyes regenerating after retinal detachment were detected in newts exposed to clinorotation. The cells divided, were hypertrophied, and their processes were thickened. Such changes suggested reactive gliosis and were more significant in animals exposed to rotation when compared with desk-top controls. Later experiments onboard the Russian biosatellite Bion-11 showed similar changes in the retinas that were regenerating in a two-week spaceflight. In the Bion-11 animals, GFAP, the major structural protein of retinal macroglial cells, was found to be upregulated. In a more recent experiment onboard Foton-M3 (2007), GFAP expression in retinas of space-flown, ground control (kept at 1 g), and basal control (sacrificed on launch day) newts was quantified, using microscopy, immunohistochemistry, and digital image analysis. A low level of immunoreactivity was observed in basal controls. In contrast, retinas of space-flown animals showed greater GFAP immunoreactivity associated with both an increased cell number and a higher thickness of intermediate filaments. This, in turn, was accompanied by up-regulation of stress protein (HSP90) and growth factor (FGF2) expressions. It can be postulated that such a response of Müller cells was to mitigate the retinal stress in newts exposed to microgravity. Taken together, the data suggest that the retinal population of macroglial cells could be sensitive to gravity changes and that in space it can react by enhancing its neuroprotective function.     

Changes in the numbers of osteoclasts in newts under conditions of microgravity.

Intensity of osteoclastic resorption and calcium content were investigated in intact limb bones of the newts flown on board of a biosatellite Cosmos-2229 after amputation of their forelimbs and tail. Using X-ray microanalysis it was shown an increase in calcium content in the bones on 20th day after operation. Histological study revealed an activation of osteoclastic resorption on endosteal surface of long bones. The newts exposed after surgery on a biosatellite had the same level of bone mineralisation as operated ground control ones, but the increase in number of polynuclear osteoclasts was lower.