Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.     

Space Shuttle UHF communications performance evaluation

Computational investigations and experimental measurements were performed to evaluate the Space Shuttle UHF communication system performance for payload bay antenna at the proposed new location. To insure adequate communication coverage at relocated new location, the link margin for the Extravehicular Activity (EVA) astronauts and between Space Shuttle Orbiter and International Space Station (SSO-ISS) during rendezvous were analyzed. The multipath effects from the vehicle structures surrounding the antenna were investigated. The Radio Frequency (RF) electromagnetic radiation to the Orbiter Docking System (ODS) pyrotechnics was also analyzed to ensure the EMC/EMI compliances.     

The effect of small vibrations on Marangoni convection and the free surface of a liquid bridge

The effects of small vibrations on Marangoni convection were investigated experimentally using a liquid bridge of 5 cSt silicone oil with a disk diameter of 7.0 mm, and an aspect ratio close to 0.5. Experiments were performed to determine the critical temperature difference data for no vibration case and with small vibrations applied. The experimental results have shown that the effect of small vibrations on the onset of oscillatory flow is small since the critical temperature difference data for different aspect ratios were not affected by the vibrations. To clarify the surface oscillation phenomena induced by external vibrations, a 3-D numerical simulation model was also developed using a level set algorithm to predict the surface oscillations of isothermal silicone oil bridges. By subjecting the liquid bridge to small vibrations, the surface oscillation characteristics were predicted numerically, and the numerical results compared well with the predictions of an analytical model proposed previously. Furthermore, the effect of small vibrations on the surface vibration amplitude of the liquid bridge is also discussed.     

Comparison of two measurement fusion methods forKalman-filter-based multisensor data fusion

Currently there exist two commonly used measurement fusion methods for Kalman-filter-based multisensor data fusion. The first (Method I) simply merges the multisensor data through the observation vector of the Kalman filter, whereas the second (Method II) combines the multisensor data based on a minimum-mean-square-error criterion. This paper, based on an analysis of the fused state estimate covariances of the two measurement fusion methods, shows that the two measurement fusion methods are functionally equivalent if the sensors used for data fusion, with different and independent noise characteristics, have identical measurement matrices. Also presented are simulation results on state estimation using the two measurement fusion methods, followed by the analysis of the computational advantages of each method     

Parallel resonant converter with LLC-type commutation

It is shown that by using a proper transformation of state variables, the third-order system of the parallel resonant converter (PRC) with LLC-type commutation can be analyzed by means of a two-dimensional state-plane diagram. A set of characteristic curves which can be used for the converter design is derived from the analysis. It is shown from these curves that the converter possesses more desirable features than the conventional PRC     

Analysis and Design of Series Resonant Converter by State-Plane Diagram

Analysis based on the state-plane diagram is given for series resonant converters operating in the frequency range 0.5 ? fs/fo ? 1.0. When the voltages and currents in the converter are normalized, design parameters take on special geometric meanings in the normalized state diagram. Examples of converter design using graphical methods are given for the cases of ? and ? control. Control characteristics of the converter operating in the continuous conduction mode are derived. The concept of the energy reflection coefficient is introduced as a measure of power transfer efficiency in the converter design.     

Orientations of LASCO Halo CMEs and their connection to the flux rope structure of interplanetary CMEs

Coronal mass ejections (CMEs) observed near the Sun via LASCO coronographic imaging are the most important solar drivers of geomagnetic storms. ICMEs, their interplanetary, near-Earth counterparts, can be detected in situ, for example, by the Wind and ACE spacecraft. An ICME usually exhibits a complex structure that very often includes a magnetic cloud (MC). They can be commonly modelled as magnetic flux ropes and there is observational evidence to expect that the orientation of a halo CME elongation corresponds to the orientation of the flux rope. In this study, we compare orientations of elongated CME halos and the corresponding MCs, measured by Wind and ACE spacecraft. We characterize the MC structures by using the Grad–Shafranov reconstruction technique and three MC fitting methods to obtain their axis directions. The CME tilt angles and MC fitted axis angles were compared without taking into account handedness of the underlying flux rope field and the polarity of its axial field. We report that for about 64% of CME–MC events, we found a good correspondence between the orientation angles implying that for the majority of interplanetary ejecta their orientations do not change significantly (less than 45 deg rotation) while travelling from the Sun to the near-Earth environment.     

A regional tropospheric model for airborne GPS applications

A regional tropospheric model can be constructed using surveys from GPS ground networks. Using this model the tropospheric delays of a kinematic station within the region can be interpolated. However, such a model is generally not suitable for an airborne platform high above the ground networks. In this paper, a method of constructing a regional tropospheric model for airborne GPS applications is described. First, the kinematic station in the air is projected onto the ground. Then the tropospheric delays at projected point are interpolated from those of the ground networks. Finally, the tropospheric delays at projected point are extended upward to the airborne platform using pressure and temperature gradients and humidity exponential function. For validation of this method, the data of airborne campaign carried out by BKG in cooperation with GFZ and BGR 2008 in the northern Alps are used. The results show that GPS kinematic positioning precision in height component can be improved using this method.     

The Magnetic Electron Ion Spectrometer (MagEIS) Instruments Aboard the Radiation Belt Storm Probes (RBSP) Spacecraft

This paper describes the Magnetic Electron Ion Spectrometer (MagEIS) instruments aboard the RBSP spacecraft from an instrumentation and engineering point of view. There are four magnetic spectrometers aboard each of the two spacecraft, one low-energy unit (20–240 keV), two medium-energy units (80–1200 keV), and a high-energy unit (800–4800 keV). The high unit also contains a proton telescope (55 keV–20 MeV). The magnetic spectrometers focus electrons within a selected energy pass band upon a focal plane of several silicon detectors where pulse-height analysis is used to determine if the energy of the incident electron is appropriate for the electron momentum selected by the magnet. Thus each event is a two-parameter analysis, an approach leading to a greatly reduced background. The physics of these instruments are described in detail followed by the engineering implementation. The data outputs are described, and examples of the calibration results and early flight data presented.