Raman imagery: a new approach to assess the geochemical maturity and biogenicity of permineralized precambrian fossils

Laser-Raman imagery is a non-intrusive, non-destructive analytical technique, recently introduced to Precambrian paleobiology, that can be used to demonstrate a one-to-one spatial correlation between the optically discernible morphology and kerogenous composition of permineralized fossil microorganisms. Made possible by the submicron-scale resolution of the technique and its high sensitivity to the Raman signal of carbonaceous matter, such analyses can be used to determine the chemical-structural characteristics of organic-walled microfossils and associated sapropelic carbonaceous matter in acid-resistant residues and petrographic thin sections. Here we use this technique to analyze kerogenous microscopic fossils and associated carbonaceous sapropel permineralized in 22 unmetamorphosed or little-metamorphosed fine-grained chert units ranging from approximately 400 to approximately 2,100 Ma old. The lineshapes of the Raman spectra acquired vary systematically with five indices of organic geochemical maturation: (1) the mineral-based metamorphic grade of the fossil-bearing units; (2) the fidelity of preservation of the fossils studied; (3) the color of the organic matter analyzed; and both the (4) H/C and (5) N/C ratios measured in particulate kerogens isolated from bulk samples of the fossil-bearing cherts. Deconvolution of relevant spectra shows that those of relatively well-preserved permineralized kerogens analyzed in situ exhibit a distinctive set of Raman bands that are identifiable also in hydrated organic-walled microfossils and particulate carbonaceous matter freed from the cherts by acid maceration. These distinctive Raman bands, however, become indeterminate upon dehydration of such specimens. To compare quantitatively the variations observed among the spectra measured, we introduce the Raman Index of Preservation, an approximate measure of the geochemical maturity of the kerogens studied that is consistent both with the five indices of organic geochemical alteration and with spectra acquired from fossils experimentally heated under controlled laboratory conditions. The results reported provide new insight into the chemical-structural characteristics of ancient carbonaceous matter, the physicochemical changes that accompany organic geochemical maturation, and a new criterion to be added to the suite of evidence by which to evaluate the origin of minute fossil-like objects of possible but uncertain biogenicity.     

Fluxes of Quasi-trapped Electrons with Energies >0.08 MeV in the Near-Earth Space on Drift Shells <Emphasis Type="Italic">L</Emphasis> < 2

We study the characteristics of fluxes of electrons with energy >80 keV in the near-Earth space regions corresponding to the drift shells L = 1.7, 1.4, and 1.1 observed during the entire period of the GRIF experiment onboard the Spectr module of the Mir orbital station from October 1995 to June 1997. The obtained geographic maps of the distribution of electron fluxes at the height of the station flight (400 km) and, also, the estimates of the spectra indicate that the South-Atlantic Anomaly provides for a mechanism of stable replenishment for shells with L < 1.5. The mechanism of stable replenishment of shells with L < 1.5 may be due to the scattering, in the residual atmosphere, of electrons from the inner radiation belt precipitating into the region of the South-Atlantic Anomaly.     

Discovery of a new chert-permineralized microbiota in the Proterozoic Buxa Formation of the Ranjit window, Sikkim, northeast India, and its astrobiological implications

For the foreseeable future, the search for evidence of past life in rocks acquired from other planets will be constrained by the amount of sample available and by the fidelity of preservation of any fossils present. What amount of rock is needed to establish the existence of past life? To address this question, we studied a minute amount of rock collected from cherty dolomites of the Proterozoic Buxa Formation in the metamorphically altered tectonically active northeastern Himalaya. In particular, we investigated 2 small petrographic thin sections-one from each of 2 bedded chert horizons exposed in the Ranjit River stratigraphic section northwest of Rishi, Sikkim, India-that together comprise an area of approximately 5 cm(2) (about the size of a US postage stamp) and have a total rock weight of approximately 0.1 g. Optical microscopy, confocal laser scanning microscopy, and Raman spectroscopy and imagery demonstrate that each of the thin sections contains a rich assemblage of 3-dimensionally permineralized organic-walled microfossils. This study, the first report of Proterozoic microfossils in units of the Ranjit tectonic window, demonstrates that firm evidence of early life can be adduced from even a minuscule amount of fossil-bearing ancient rock.     

Construction of theories of motion, ephemerides, and databases for natural satellites of planets

A knowledge base for natural satellites of planets is created. On the basis of observations, new numerical models of motion are constructed for all 96 outer satellites of Jupiter, Saturn, Uranus, and Neptune. A special database is compiled from all available observational data for natural satellites of planets, as well as a bibliographical database and information system of physical and orbital parameters of planets and satellites. The tools for calculations of ephemerides of all natural satellites (except for the Moon) of planets are developed. They represent the final result of studies and actually incorporate the entire knowledge about dynamics of the satellites of planets. Also developed are special ephemerides in order to observe singular phenomena in the apparent motion of the satellites of planets. A computer environment and the Internet allowed us to concentrate all above-listed options in a single toolkit easily available to any user in the world.     

Viscosity effects on weak irregular reflection of shock waves in steady flow

The viscosity effects on weak shock wave reflection are investigated with the Navier–Stokes and DSMC flow solvers. It is shown that the viscosity plays a crucial role in the vicinity of three-shock intersection. Instead of a singular triple point, in viscous flow there is a smooth three shock transition zone, where one-dimensional shock jump relations cannot be applied. At the flow parameters corresponding to the von Neumann reflection, when no inviscid three-shock solution exists, the computations predict an irregular shock-wave configuration similar to that observed previously in experiments. The existence of a viscous zone in the region of shock-wave interaction allows a continuous transition from the parameters behind the Mach stem to the parameters behind the reflected shock, which is impossible in the inviscid three-shock theory. Results of numerical simulations agree qualitatively with the conclusions of the approximate viscous theory, which describes the flow in the vicinity of the three-shock intersection.     

High-energy gamma-astronomy with large underground detectors

Large underground (underwater) detectors (such as ‘Baikal’ and DUMAND) are discussed for their possible use as gamma-ray telescopes. The signal is caused by high-energy muons (E _μ ? 30–100 TeV) produced by the primary gamma-radiation in the Earth's atmosphere. The production of gamma-rays in the source through the reaction p + p → π ⁰ + X is calculated for a low density target of arbitrary thickness taking the electromagnetic cascade into account. The muon production by gamma-rays in the Earth's atmosphere is calculated using three processes: (i) photoproduction of π- and K-mesons followed by decay to muons, (ii) the direct production of μ⁺ μ^?-pairs: γ + Z → Z + μ ⁺ + μ^?, and (iii) photoproduction of charmed particles. It is shown that for thin sources with a flat spectrum (integral exponent γ = 1.1) a large (S = 0.1 km²) underground detector can detect both neutrinos and gamma-quanta generated by the source. Finally we compare the performances of underground detectors with S = 0.1 km² for the search of gamma sources at E = 100 TeV with those of the previously proposed 1 km² EAS array.     

ASAR imaging for coastal upwelling in the Baltic Sea

Analysis of Envisat Advanced Synthetic Aperture Radar (ASAR) and Aqua/Terra Moderate Imaging Spectrometer (MODIS) infrared (IR) imagery of coastal upwelling in the southeastern Baltic Sea is presented. It is found that upwelling features are well distinct in the SAR images, and the leading imaging mechanism appears to be the change of the marine atmospheric boundary layer (MABL) stratification over the sea surface temperature (SST) front. This finding is supported by model calculations of the MABL transformation supplemented with the SAR backscatter calculations based on the CMOD4 model. In addition an empirical dependence of the SAR contrasts over the upwelling region on the wind speed and the SST drop is suggested. Finally, surface slicks accumulated in the sea surface current convergence zones generate additional distinct features in SAR imagery. This effect is interpreted within the framework of the coastal current circulation model based on analysis of the SST snapshot.     

Measurements of high-energy neutron and proton fluxes on-board “Mir-Spectr” orbital complex

The measurements of high-energy neutron (with energies 30–300 MeV) and proton (with energies 1–200 MeV) fluxes are being conducted on-board “Mir-Spectr” orbital complex. Neutrons are detected by the undirected (FOV 4π sr) scintillator spectrometer, consisting of 4 identical CsI(Tl) detector units (the effective area for neutrons 30 cm²). The gamma-quanta, which can be also detected by this instrument, are separated from neutrons by the analysis of the scintillator output pulse shape. To exclude registration of charged particles an anticoincidence plastic scintillator shield is realized in each detector unit. The proton fluxes are measured by the telescope based on 3 semiconductor detectors with small geometry factor (1 cm²×sr). As the first result of the experiment the upper limit of the integral flux of local and albedo neutrons in the equatorial region (L<1.1) was estimated. The results of this measurements can be useful for the radiation security. Also, the neutrons of solar flares can be detected in this experiment.     

Gypsum-permineralized microfossils and their relevance to the search for life on Mars

Abstract Orbital and in situ analyses establish that aerially extensive deposits of evaporitic sulfates, including gypsum, are present on the surface of Mars. Although comparable gypsiferous sediments on Earth have been largely ignored by paleontologists, we here report the finding of diverse fossil microscopic organisms permineralized in bottom-nucleated gypsums of seven deposits: two from the Permian (～260?Ma) of New Mexico, USA; one from the Miocene (～6?Ma) of Italy; and four from Recent lacustrine and saltern deposits of Australia, Mexico, and Peru. In addition to presenting the first report of the widespread occurrence of microscopic fossils in bottom-nucleated primary gypsum, we show the striking morphological similarity of the majority of the benthic filamentous fossils of these units to the microorganisms of a modern sulfuretum biocoenose. Based on such similarity, in morphology as well as habitat, these findings suggest that anaerobic sulfur-metabolizing microbial assemblages have changed relatively little over hundreds of millions of years. Their discovery as fossilized components of the seven gypsiferous units reported suggests that primary bottom-nucleated gypsum represents a promising target in the search for evidence of past life on Mars. Key Words: Confocal laser scanning microscopy-Gypsum fossils-Mars sample return missions-Raman spectroscopy-Sample Analysis at Mars (SAM) instrument-Sulfuretum. Astrobiology 12, 619-633.