Survival and death of the haloarchaeon Natronorubrum strain HG-1 in a simulated martian environment

Halophilic archaea are of interest to astrobiology due to their survival capabilities in desiccated and high salt environments. The detection of remnants of salty pools on Mars stimulated investigations into the response of haloarchaea to martian conditions. Natronorubrum sp. strain HG-1 is an extremely halophilic archaeon with unusual metabolic pathways, growing on acetate and stimulated by tetrathionate. We exposed Natronorubrum strain HG-1 to ultraviolet (UV) radiation, similar to levels currently prevalent on Mars. In addition, the effects of low temperature (4, −20, and −80 °C), desiccation, and exposure to a Mars soil analogue from the Atacama desert on the viability of Natronorubrum strain HG-1 cultures were investigated. The results show that Natronorubrum strain HG-1 cannot survive for more than several hours when exposed to UV radiation equivalent to that at the martian equator. Even when protected from UV radiation, viability is impaired by a combination of desiccation and low temperature. Desiccating Natronorubrum strain HG-1 cells when mixed with a Mars soil analogue impaired growth of the culture to below the detection limit. Overall, we conclude that Natronorubrum strain HG-1 cannot survive the environment currently present on Mars. Since other halophilic microorganisms were reported to survive simulated martian conditions, our results imply that survival capabilities are not necessarily shared between phylogenetically related species.     

Some problems of selection and evaluation of the Martian suit enclosure concept

One of the most important tasks for preparation of a future manned mission to Mars is to create a space suit, which ensures efficient and safe operation of the man on the planet surface.

The concept of space suit (SS) utilisation on the Mars surface will be determined mainly by the Mars mission scenario. Currently the preference is given to utilisation of robotics with the crew driving a Mars rover vehicle, whereby the suit will be used solely as an additional safety means.

However, one cannot exclude the necessity of a durable self-contained stay of the man outside a pressurised compartment, to pick up, for instance, soil samples or do certain repair work in case of an emergency.

The requirements to the Mars suit and especially to the personal self-contained life support system (LSS) will depend in many respects on the Mars environmental conditions, the space vehicle system concept and performance characteristics, the airlock and its interface design, the availability of expendable elements for the LSS, etc.

The paper reviews principal problems, which have to be solved during development of the Martian suit. A special attention is paid to the issue of suited man mobility during traversing on the planet surface.

The paper also reviews the arguments for application of a suit semi-rigid design concept and evaluates potentialities of using certain elements of the existing “Orlan” type suit.

The paper presents results of a number of studies on selection of the planetary SS enclosure concept and on experimental evaluation of mobility of the lower torso and leg enclosures in conjunction with a specially designed prototype model (tentative model) of the SS enclosure.     

The Soviet/Russian spacesuit history: Part III—The European connection

The European spacesuit system (ESSS) initiated by the European Space Agency (ESA) in the late 1980s had many similarities with the Soviet/Russian ORLAN spacesuit system, due to the Hermes system requirements. First, direct contacts in 1989 permitted closer comparison of the two suit systems, and soon the ORLAN manufacturer Zvezda could be contracted as support to the European spacesuit team. In particular, the suit enclosure design and predevelopment testing and operational analysis were performed in close cooperation between Zvezda and the European team under Dornier.

With the changing system requirements and a closer cooperation between ESA and the new Russian Space Agency (RKA) a new joint spaceplane/stations mission scenario came about. This scenario could be served by one spacesuit system, EVA SUIT 2000, which was to be jointly developed by a team headed by Zvezda and Dornier for ESA and RKA. ORLAN-DMA and ESSS experience and hardware were the initial platforms for these activities to create a new generation spacesuits for the Mir 2 and later the ISSs.

A suit demonstrator was manufactured and tested by the end of 1994 when ESA stopped its spacesuit development activities and the joint EVA SUIT 2000 project was terminated. However, many of the features designed, manufactured and tested for the EVA SUIT 2000 were then implemented by Zvezda in the new Russian spacesuit system ORLAN-M, now in full operation onboard the ISS.     

Electric discharge in the Martian atmosphere,Paschen curves and implications for future missions

Electric discharge between two electrically charged surfaces occurs at a well-defined, gas-dependent combination of atmospheric pressure and the distance between those two surfaces, as described by Paschen’s law. The understanding of when the discharge will occur in the conditions present on Mars is essential for designing space-flight hardware that will operate on the Martian surface as well as understanding electrical discharge processes occurring in the Martian atmosphere. Here, we present experimentally measured Paschen curves for a gas mixture representative of the Martian atmosphere and compare our results to breakdown voltages of carbon dioxide, nitrogen, and helium as measured with our system and from the literature. We will discuss possible implications for instrument development as well as implications for processes in the Martian atmosphere. The DC voltage at which electric discharge occurred between two stainless steel spheres was measured at pressures from 10⁻² to 100 torr in all gases. We measured a minimum voltage for discharge in the Mars ambient atmosphere of 410 ± 10 V at 0.3 torr cm. As an application, the breakdown properties of space-qualified, electrical wires to be used in the Sample Analysis at Mars (SAM) instrument suite on the Mars Science Laboratory (MSL) were studied.     

Mitigation of the impact of terrestrial contamination on organic measurements from the Mars Science Laboratory

The objective of the 2009 Mars Science Laboratory (MSL), which is planned to follow the Mars Exploration Rovers and the Phoenix lander to the surface of Mars, is to explore and assess quantitatively a site on Mars as a potential habitat for present or past life. Specific goals include an assessment of the past or present biological potential of the target environment and a characterization of its geology and geochemistry. Included in the 10 investigations of the MSL rover is the Sample Analysis at Mars (SAM) instrument suite, which is designed to obtain trace organic measurements, measure water and other volatiles, and measure several light isotopes with experiment sequences designed for both atmospheric and solid-phase samples. SAM integrates a gas chromatograph, a mass spectrometer, and a tunable laser spectrometer supported by sample manipulation tools both within and external to the suite. The sub-part-per-billion sensitivity of the suite for trace species, particularly organic molecules, along with a mobile platform that will contain many kilograms of organic materials, presents a considerable challenge due to the potential for terrestrial contamination to mask the signal of martian organics. We describe the effort presently underway to understand and mitigate, wherever possible within the resource constraints of the mission, terrestrial contamination in MSL and SAM measurements.