The role of DNA repair on cell killing by charged particles.

It can be noted that it is not simple double strand breaks (dsb) but the non-reparable breaks that are associated with high biological effectiveness in the cell killing effect for high LET radiation. Here, we have examined the effectiveness of fast neutrons and low (initial energy = 12 MeV/u) or high (135 MeV/u) energy charged particles on cell death in 19 mammalian cell lines including radiosensitive mutants. Some of the radiosensitive lines were deficient in DNA dsb repair such as LX830, M10, V3, and L5178Y-S cells and showed lower values of relative biological effectiveness (RBE) for fast neutrons if compared with their parent cell lines. The other lines of human ataxia-telangiectasia fibroblasts, irs 1, irs 2, irs 3 and irs1SF cells, which were also radiosensitive but known as proficient in dsb repair, showed moderated RBEs. Dsb repair deficient mutants showed low RBE values for heavy ions. These experimental findings suggest that the DNA repair system does not play a major role against the attack of high linear energy transfer (LET) radiations. Therefore, we hypothesize that a main cause of cell death induced by high LET radiations is due to non-reparable dsb, which are produced at a higher rate compared to low LET radiations.     

Oxygen line mapping of SN 1006 with Suzaku

SN 1006 is one of the supernova remnants (SNRs) with relatively low-temperature electrons, considering the young age of just 1000 years. We carried out SN 1006 mapping observations with the X-ray Imaging Spectrometers (XIS) and the Hard X-ray Detector (HXD) onboard Suzaku, the fifth Japanese X-ray satellite. Thanks to the excellent spectral resolution of XIS in the soft X-ray band, H-like and He-like oxygen emission lines were clearly detected, and we could make a map of the line intensity, and as well as a flux and the photon index of nonthermal component. We found that these parameters have spatial dependences from region to region in the SNR; the north region is bright in nonthermal, while dim in thermal; the east region is bright in both nonthermal and thermal; the inner region shows dim nonthermal and bright thermal emission. The photon index is the smallest in the north region.     

Repair of DNA double-strand breaks and cell killing by charged particles.

It has been suggested that it is not simple double-strand breaks (dsb) but the non-reparable breaks which correlate well with the high biological effectiveness of high LET radiations for cell killing (Kelland et al., 1988; Radford, 1986). We have compared the effects of charged particles on cell death in 3 pairs of cell lines which are normal or defective in the repair of DNA dsbs. For the cell lines SL3-147, M10, and SX10 which are deficient in DNA dsb repair, RBE values were close to unity for cell killing induced by charged particles with linear energy transfer (LET) up to 200 keV/micrometer and were even smaller than unity for the LET region greater than 300 keV/micrometer. The inactivation cross section (ICS) increased with LET for all 3 pairs. The ICS of dsb repair deficient mutants was always larger than that of their parents for all the LET ranges, but with increasing LET the difference in ICS between the mutant and its parent became smaller. Since a small difference in ICS remained at LET of about 300 keV/micrometer, dsb repair may still take place at this high LET, even if its role is apparently small. These results suggest that the DNA repair system does not play a major role in protection against the attack of high LET radiations and that a main muse of cell death is non-reparable dsb which are produced at a higher yield compared with low LET radiations. No correlation was observed between DNA content or nuclear area and ICS.     

Atmospheric response to a Hot SST Event in November 2006 as observed by the AIRS instrument

Using Atmospheric Infrared Sounder (AIRS) products of atmospheric temperature and geopotential height, we investigate the atmospheric response to HE0611, which was found and investigated by [Qin, H., Kawamura, H., Sakaida, F., Ando, K. A case study of the tropical Hot Event in November 2006 (HE0611) using a geostationary meteorological satellite and the TAO/TRITON mooring array. J. Geophys. Res. 113, C08045, doi: 10.1029/2007JC004640, 2008]. HE0611 was formed by connecting two very high SST areas, HE0611-East and HE0611-West. The period-mean atmosphere temperatures at levels of 925 and 850 hPa in HE0611-West are higher, by about 0.5 K, than those in WE0611-East while the atmospheric temperatures at middle to high levels (700–300 hPa) are higher in HE0611-East. The period-mean geopotential heights HE0611-East are much lower than those in HE0611-West for the levels from the surface to 400 hPa. The mean geopotential heights from 400 hPa to 200 hPa are higher in HE0611-East. In the middle and high layers over HE0611-West, the atmosphere temperatures gradually decrease from 7th to 17th, and then increase significantly. The increase in HE0611-East starts from 15th November, which is earlier than that of HE0611-West. The geopotential heights in the high layer of both the areas also show corresponding behaviors. The lagged atmospheric response in the western part is confirmed by the correlation analysis. It emerges that the atmospheric response to HE0611 is well organized and associated with deep convention in HE0611-East and subsidence in HE0611-West. These are also consistent with the HE0611 features and evolution revealed by earlier HE studies.     

Influence of gravity on the circadian timing system.

The circadian timing system (CTS) is responsible for daily temporal coordination of physiological and behavioral functions both internally and with the external environment. Experiments in altered gravitational environments have revealed changes in circadian rhythms of species ranging from fungi to primates. The altered gravitational environments examined included both the microgravity environment of spaceflight and hyperdynamic environments produced by centrifugation. Acute exposure to altered gravitational environments changed homeostatic parameters such as body temperature. These changes were time of day dependent. Exposure to gravitational alterations of relatively short duration produced changes in both the homeostatic level and the amplitude of circadian rhythms. Chronic exposure to a non-earth level of gravity resulted in changes in the period of the expressed rhythms as well as in the phase relationships between the rhythms and between the rhythms and the external environment. In addition, alterations in gravity appeared to act as a time cue for the CTS. Altered gravity also affected the sensitivity of the pacemaker to other aspects of the environment (i.e., light) and to shifts of time cues. Taken together, these studies lead to the conclusion that the CTS is indeed sensitive to gravity and its alterations. This finding has implications for both basic biology and space medicine.     

Deriving the Absolute Reflectance of Lunar Surface Using SELENE (Kaguya) Multiband Imager Data

The absolute reflectance of the Moon has long been debated because it has been suggested (Hillier et al. in Icarus 151:205–225, 1999) that there is a large discrepancy between the absolute reflectance of the Moon derived from Earth-based telescopic data and that derived from remote-sensing data which are calibrated using laboratory-measured reflectance spectra of Apollo 16 bulk soil 62231. Here we derive the absolute reflectance of the lunar surface using spectral data newly acquired by SELENE (Kaguya) Multiband Imager and Spectral Profiler. The results indicate that the reflectance of the Apollo 16 standard site, which has been widely used as an optical standard in previous Earth-based telescopic and remote-sensing observations derived by Multiband Imager, is 47% at 415 nm and 67% to 76% at 750 to 1550 nm of the value for the Apollo 16 mature soil measured in an Earth-based laboratory. The data also suggest that roughly 60% of the difference is caused by the difference in soil composition and/or maturity between the 62231 sampling site and the Apollo 16 standard site and that the remaining 40% difference can be explained by the difference between the compaction states of the laboratory and the actual lunar surface. Consideration of the compaction states of the surface soil demonstrates its importance for understanding the spectral characteristics of the lunar surface. We also explain and evaluate data analysis procedures to derive reflectance from Multiband Imager data.     

New results and questions of lunar exploration from SELENE,Chang’E-1, Chandrayaan-1 and LRO/LCROSS

The moon has longstanding questions such as lunar environments, origin, formation and evolution, magnetization of crustal rocks, internal structure and possible life. The recent lunar missions, e.g., SELenological and ENgineering Explorer “KAGUYA” (SELENE), Chang’E-1, Chandrayaan-1, and Lunar Reconnaissance Orbiter/Lunar CRater Observation and Sensing Satellite (LRO/LCROSS), have provided new opportunities to explore and understand these issues. In this paper, we reviewed and presented the results and findings in the fields of lunar gravity, magnetic field, atmosphere, surface geomorphology and compositional variations, volcano, craters, internal structure, water and life science from new lunar exploration missions. In addition, the new objectives and scientific questions on lunar explorations in near future are presented and discussed.     

Identification of power analysis models for ETS-III operation

This paper describes the identification of power analysis models of BOP (the Bus Equipment Operation Analysis Program) for ETS-III operation. The ETS-III (Engineering Test Satellite III) was launched in September 1982. BOP simulations are to provide prior confirmation for safe operation in terms of electrical power and thermal control situations. It is necessary to identify power analysis model first and improve the model for higher accuracy. Studies for model identification have been done by using flight data and simulation results. As a result of these studies, the power analysis model meets well with the actual ETS-III power situation and the aimed accuracy of the model has been achieved.