Inhibition of reproductive growth of Arabidopsis in airtight vessels

Airtight vessels have various advantages for space experiments. However, Arabidopsis thaliana plants scarcely produced seeds when grown in such vessels. The mechanism by which reproductive growth is inhibited in airtight vessels was studied. The length of the flower stalk was shorter when the plants were grown in airtight vessels. Thus, there was a possibility that the inhibition of reproductive growth was due to the inhibition of vegetative growth. However, even when the plants which has grown under non-airtight conditions and has reached to the flowering stage were transferred to airtight vessels, silique formation was inhibited, suggesting that the airtight environment directly influences reproductive growth. In airtight vessels, anther dehiscence was inhibited, which appears to be the cause of inhibition of silique formation and seed development. Reproductive growth recovered when silica gel was added to the vessels. These results suggest that in airtight vessels, high humidity causes a suppression of anther dehiscence, resulting in the inhibition of reproductive growth. Therefore, the control of humidity by ventilation should be taken into consideration in designing a growth chamber for space experiments.     

Effects of gravistimuli on osmoregulation in azuki bean epicotyls

The effects of hypergravity on growth and osmoregulation were examined in dark-grown azuki bean epicotyls. Elongation growth of epicotyls was promptly suppressed by hypergravity at 300g. On the contrary, the increase in fresh weight of epicotyls during incubation was not suppressed by hypergravity at 300g at least up to 6 h. Also, the level of total osmotic solutes increased during epicotyl growth for 6 h, which was not affected by hypergravity. These results suggest that azuki bean epicotyls are capable of maintaining osmoregulation even under 300g conditions for a short period. On the other hand, the increase in fresh weight of epicotyls was suppressed, in addition to suppression of elongation growth, when seedlings were treated with 300g for 24 h. The increase in level of total osmotic solutes was also inhibited by 24 h hypergravity treatment, which was accounted by the reduced levels of organic solutes, such as sugars and amino acids. Furthermore, the dry weight of seeds decreased during incubation for 24 h, but the decrease was inhibited by hypergravity at 300g. Hypergravity treatment at 300g for 24 h also increased the pH value of apoplastic solution in epicotyls. Taken together, these results suggest that the translocation of organic solutes from the seed to epicotyls is inhibited by prolonged hypergravity treatment, which may underlie the suppression of epicotyl growth, and that the breakdown of H⁺ gradient across the plasma membrane in epicotyl cells may be at least partly involved in the reduction of organic solute accumulation under hypergravity conditions.     

Mercury Dust Monitor (MDM) Onboard the Mio Orbiter of the BepiColombo Mission

Space Science Reviews - In this chapter, we review the contribution of space missions to the determination of the elemental and isotopic composition of Earth, Moon and the terrestrial planets, with...     

Meteoroids as One of the Sources for Exosphere Formation on Airless Bodies in the Inner Solar System

Space Science Reviews -     

Study on variable-shape supersonic inlets and missiles with MRD device

This paper proposes a new aerodynamic device, which was designated multi-row-disk (MRD). This device has a cone and stabilizer disks being arranged in the axial direction. This device can arbitrarily change its aerodynamic characteristics by translating stabilizer disks. In the first part of this paper, the effect of several nose shape configurations including the MRD device on the aerodynamic characteristics is reported. By increasing the number of stabilizer disks, zero-lift drag and induced drag can be reduced. It was also found that putting cavities on the conical surface is effective for improving longitudinal static stability. In the second part, the effect of cavity flow instability on pressure and strain oscillation is reported. We drew the design criterion that the configuration of stabilizer disks should be determined not to couple the 1st mode with pressure oscillation frequency, which can be predicted with Rossiter's formula.     

Improvements in and actual performance of the Plant Experiment Unit onboard Kibo,the Japanese experiment module on the international space station

In 2004, Japan Aerospace Exploration Agency developed the engineered model of the Plant Experiment Unit and the Cell Biology Experiment Facility. The Plant Experiment Unit was designed to be installed in the Cell Biology Experiment Facility and to support the seed-to-seed life cycle experiment of Arabidopsis plants in space in the project named Space Seed. Ground-based experiments to test the Plant Experiment Unit showed that the unit needed further improvement of a system to control the water content of a seedbed using an infrared moisture analyzer and that it was difficult to keep the relative humidity inside the Plant Experiment Unit between 70 and 80% because the Cell Biology Experiment Facility had neither a ventilation system nor a dehumidifying system. Therefore, excess moisture inside the Cell Biology Experiment Facility was removed with desiccant bags containing calcium chloride. Eight flight models of the Plant Experiment Unit in which dry Arabidopsis seeds were fixed to the seedbed with gum arabic were launched to the International Space Station in the space shuttle STS-128 (17A) on August 28, 2009. Plant Experiment Unit were installed in the Cell Biology Experiment Facility with desiccant boxes, and then the Space Seed experiment was started in the Japanese Experiment Module, named Kibo, which was part of the International Space Station, on September 10, 2009 by watering the seedbed and terminated 2 months later on November 11, 2009. On April 19, 2010, the Arabidopsis plants harvested in Kibo were retrieved and brought back to Earth by the space shuttle mission STS-131 (19A). The present paper describes the Space Seed experiment with particular reference to the development of the Plant Experiment Unit and its actual performance in Kibo onboard the International Space Station. Downlinked images from Kibo showed that the seeds had started germinating 3 days after the initial watering. The plants continued growing, producing rosette leaves, inflorescence stems, flowers, and fruits in the Plant Experiment Unit. In addition, the senescence of rosette leaves was found to be delayed in microgravity.     

Modeling PSBL high speed ion beams observed by Cluster and Double Star

On October 8, 2004, the Cluster and Double Star spacecraft crossed the near-Earth (12–19 R_E) magnetotail neutral sheet during the recovery phase of a small, isolated substorm. Although they were separated in distance by ∼7 R_E and in time by ∼30 min, both Cluster and Double Star observed steady, but highly structured Earthward moving >1000 km/s high speed H⁺ beams in the PSBL. This paper utilizes a global magnetohydrodynamic (MHD) simulation driven by Wind spacecraft solar wind input to model the large-scale structure of the PSBL and large-scale kinetic (LSK) particle tracing calculations to investigate the similarities and differences in the properties of the observed beams. This study finds that the large-scale shape of the PSBL is determined by the MHD configuration. On smaller scales, the LSK calculations, in good qualitative agreement with both Cluster and Double Star observations, demonstrated that the PSBL is highly structured in both time and space, on time intervals of less than 2 min, and spatial distances of the order of 0.2–0.5 R_E. This picture of the PSBL is different from the ordered and structured region previously reported in observations.