The role of DNA repair on cell killing by charged particles.

It can be noted that it is not simple double strand breaks (dsb) but the non-reparable breaks that are associated with high biological effectiveness in the cell killing effect for high LET radiation. Here, we have examined the effectiveness of fast neutrons and low (initial energy = 12 MeV/u) or high (135 MeV/u) energy charged particles on cell death in 19 mammalian cell lines including radiosensitive mutants. Some of the radiosensitive lines were deficient in DNA dsb repair such as LX830, M10, V3, and L5178Y-S cells and showed lower values of relative biological effectiveness (RBE) for fast neutrons if compared with their parent cell lines. The other lines of human ataxia-telangiectasia fibroblasts, irs 1, irs 2, irs 3 and irs1SF cells, which were also radiosensitive but known as proficient in dsb repair, showed moderated RBEs. Dsb repair deficient mutants showed low RBE values for heavy ions. These experimental findings suggest that the DNA repair system does not play a major role against the attack of high linear energy transfer (LET) radiations. Therefore, we hypothesize that a main cause of cell death induced by high LET radiations is due to non-reparable dsb, which are produced at a higher rate compared to low LET radiations.     

Transition of Planar Rotational Motion of an Asymmetric Spacecraft into Oscillatory Motion at Its Reentry into the Atmosphere

The motion of a spacecraft with small asymmetry relative to its center of mass is considered. The restoring aerodynamic moment of the spacecraft is described by the Fourier series in terms of the angle of attack with the two first sinusoidal and the first cosinusoidal terms. A solution for the angle of attack in the undisturbed rotational motion is found. The analytical expression is obtained for the integral of action taken along the separatrices that separate the rotational and oscillatory regions of the phase portrait of a system. The transition of the spacecraft's motion from planar rotational to oscillatory is investigated. This transition is caused by a slow variation of moment characteristic coefficients, as well as by the presence of small asymmetry and damping and slow variation of their coefficients. Analytical formulas are obtained for determining the times of transition from rotational to oscillatory motion, as well as for the critical angular velocity of beyond-the-atmosphere rotation. When this critical velocity is exceeded, body rotation proceeds for a long time interval (planar autorotation arises).     

The heliolongitudinal distribution of solar flares associated with solar proton events.

We find that the heliolongitudinal distribution of solar flares associated with earth-observed solar proton events is a function of the particle measurement energy. For solar proton events containing fluxes with energies exceeding 1 GeV, we find a Gaussian distribution about the probable root of the Archimedean spiral favorable propagation path leading from the earth to the sun. This distribution is modified as the detection threshold is lowered. For > 100 MeV solar proton events with fluxes > or = 10 protons (cm2-sec-ster)-1 we find the distribution becomes wider with a secondary peak near the solar central meridian. When the threshold is lowered to 10 MeV the distribution further evolves. For > 10 MeV solar proton events having a flux threshold at 10 protons (cm2-sec-ster)-1 the distribution can be considered to be a composite of two Gaussians. One distribution is centered about the probable root of the Archimedean spiral favorable propagation path leading from the earth to the sun, and the other is centered about the solar central meridian. For large flux solar proton events, those with flux threshold of 1000 (cm2-sec-ster)-1 at energies > 10 MeV, we find the distribution is rather flat for about 40 degrees either side of central meridian.     

Austrian dose measurements onboard space station MIR and the International Space Station--overview and comparison.

The Atominstitute of the Austrian Universities has conducted various space research missions in the last 12 years in cooperation with the Institute for Biomedical Problems in Moscow. They dealt with the exact determination of the radiation hazards for cosmonauts and the development of precise measurement devices. Special emphasis will be laid on the last experiment on space station MIR the goal of which was the determination of the depth distribution of absorbed dose and dose equivalent in a water filled Phantom. The first results from dose measurements onboard the International Space Station (ISS) will also be discussed. The spherical Phantom with a diameter of 35 cm was developed at the Institute for Biomedical Problems and had 4 channels where dosimeters can be exposed in different depths. The exposure period covered the timeframe from May 1997 to February 1999. Thermoluminescent dosimeters (TLDs) were exposed inside the Phantom, either parallel or perpendicular to the hull of the spacecraft. For the evaluation of the linear energy transfer (LET), the high temperature ratio (HTR) method was applied. Based on this method a mean quality factor and, subsequently, the dose equivalent is calculated according to the Q(LET infinity) relationship proposed in ICRP 26. An increased contribution of neutrons could be detected inside the Phantom. However the total dose equivalent did not increase over the depth of the Phantom. As the first Austrian measurements on the ISS dosimeter packages were exposed for 248 days, starting in February 2001 at six different locations onboard the ISS. The Austrian dosimeter sets for this first exposure on the ISS contained five different kinds of passive thermoluminescent dosimeters. First results showed a position dependent absorbed dose rate at the ISS.     

Simulation experiments of the effect of space environment on bacteriophage and DNA thin films.

The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy. To provide experimental evidence for the hypothesis that interplanetary transfer of the genetic material is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (lambda=254 nm) and high vacuum (10(-4) Pa). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.     

On Lyapunov stability analyses of the switching regulator/filtersystem

The stability design criteria for the fourth-order switching regulator/low-pass filter system are obtained directly from the solution to the resultant simultaneous equations for the Lyapunov matrix equation. The regulator/filter system is asymptotically stable if the values of the filter components are chosen to satisfy the derived Lyapunov stability design criterion for a given value of the switcher dynamic resistance     

Cosmic-ray antiprotons in the galaxy

A key issue in fundamental physics is the nature of non-baryonic dark matter in the universe. Among the candidates proposed by the particle physics community are neutralinos whose annihilation in the Galactic halo can produce antiprotons with a characteristic spectrum. Several balloon and space borne experiments have been actively looking to detect the neutralino signature in cosmic antiprotons. This task is however hampered by the existence of a secondary “background” flux of antiprotons produced in the interactions of cosmic-ray particles in the interstellar medium. A notable difficulty is that a self-consistent and complete model of cosmic-ray propagation in the Galaxy has thus far been elusive. When subtracting the secondary background from the observed data, it is therefore critical that all viable models of secondary cosmic-ray antiproton propagation be thoroughly examined, and all uncertainties stemming from plurality of the models be duly considered. In this paper we concentrate on three particular models of Diffusion, Galactic Wind, and Distributed Stochastic Reacceleration, and discuss the predicted antiproton spectrum in each model.     

Properties and performance of extended target motion analysis

The problem of target motion analysis (TMA) has been the subject of an important literature. However, present methods use data estimated by a short time analysis (azimuths, Dopplers, etc.). For far sources, the nonstationarities of the array processing outputs, induced by the sources motion, may be simply modeled. This model leads one to consider directly a spatio-temporal TMA. Then new (spatio-temporal) data can be estimated. These estimates correspond to a long time analysis. Further, note that they are estimated independently of the (classical) bearings. In this general framework, the concept of source trajectory replaces the classical instantaneous bearings. Corresponding TMA algorithms are then studied. Then the study of statistical performance is carefully studied     

Sequence comparison techniques for multisensor data fusion andtarget recognition

A new class of techniques for multisensor fusion and target recognition is proposed using sequence comparison by dynamic programming and multiple model estimation. The objective is to fuse information on the kinematic state and “nonkinematic” signature of unclassified targets, assessing the joint likelihood of all observed events for recognition. Relationships are shown to previous efforts in pattern recognition and state estimation. This research applies “classical” speech processing-related and other sequence comparison methods to moving target recognition, extends the efforts of previous researchers through improved fusion with kinematic information, relates the proposed techniques to Bayesian theory, and applies parameter identification methods to target recognition for improved understanding of the subject in general. The proposed techniques are evaluated and compared with existing approaches using the method of generalized ambiguity functions, which lends to a form of Cramer-Rao lower bound for target recognition     

Influence of long-term altered gravity on the swimming performance of developing cichlid fish: including results from the 2nd German Spacelab Mission D-2.

This study presents qualitative and quantitative data concerning gravity-dependent changes in the swimming behaviour of developing cichlid fish larvae (Oreochromis mossambicus) after a 9 resp. 10 days exposure to increased acceleration (centrifuge experiments), to reduced gravity (fast-rotating clinostat), changed accelerations (parabolic aircraft flights) and to near weightlessness (2nd German Spacelab Mission D-2). Changes of gravity initially cause disturbances of the swimming performance of the fish larvae. With prolonged stay in orbit a step by step normalisation of the swimming behaviour took place in the fish. After return to 1g earth conditions no somersaulting or looping could be detected concerning the fish, but still slow and disorientated movements as compared to controls occurred. The fish larvae adapted to earth gravity within 3-5 days. Fish seem to be in a distinct early developmental stages extreme sensitive and adaptable to altered gravity; However, elder fish either do not react or show compensatory behaviour e.g. escape reactions.