Detection of microlesions induced by heavy ions using liposomes filled with fluorescent dye.

In cells irradiation by heavy ions has been hypothesized to produce microlesions, regions of local damage. In cell membranes this damage is thought to manifest itself in the form of holes. The primary evidence for microlesions comes from morphological studies of cell membranes, but this evidence is still controversial, especially since holes also have been observed in membranes of normal, nonirradiated, cells. However, it is possible that damage not associated with histologically discernable disruptions may still occur. In order to resolve this issue, we developed a system for detecting microlesions based on liposomes filled with fluorescent dye. We hypothesized that if microlesions form in these liposomes as the result of irradiation, then the entrapped dye will leak out into the surrounding medium in a measurable way. Polypropylene vials containing suspensions of vesicles composed of either dipalmitoyl phosphatidylcholine, or a combination of egg phosphatidylcholine and cholesterol were irradiated at the Brookhaven National Laboratory using 56Fe ions at 1 GeV/amu. In several cases we obtained a significant loss of the entrapped dye above the background level. Our results suggest that holes may form in liposomes as the result of heavy ion irradiation, and that these holes are large enough to allow leakage of cell internal contents that are at least as large as a 1 nm diameter calcein molecule.     

Analyses of exobiological and potential resource materials in the Martian soil.

Potential Martian soil components relevant to exobiology include water, organic matter, evaporites, clays, and oxides. These materials are also resources for human expeditions to Mars. When found in particular combinations, some of these materials constitute diagnostic paleobiomarker suites, allowing insight to be gained into the probability of life originating on Mars. Critically important to exobiology is the method of data analysis and data interpretation. To that end we are investigating methods of analysis of potential biomarker and paleobiomarker compounds and resource materials in soils and rocks pertinent to Martian geology. Differential thermal analysis coupled with gas chromatography is shown to be a highly useful analytical technique for detecting this wide and complex variety of materials.     

The Electron Beam Instrument (F6) on Freja

The Electron Beam Instrument (F6) onFreja is the first attempt to apply the electron drift technique in a region of large ambient magnetic fields. The paper describes the operational principles, the technical realization, and the difficulties encountered in the derivation of the electric fields.     

Stellar infrared astronomy with EDISON

In this review the IR emission from circumstellar material is discussed, both of ionized gas and dust grains, and the astrophysical information that can be extracted from such observations. Some emphasis is placed on the possibilities of stellar IR astronomy using a large space-borne telescope, especially with respect to the much better spatial and spectral resolution of such a telescope compared to the current generation of ground-based and space IR telescopes.     

EUROPEAN NETWORK FOR THE NUMERICAL SIMULATION OF SPACE PLASMAS

The Cluster mission of the European Space Agency (ESA) will allow, for the first time three-dimensional measurements in key regions of the Earth's magnetosphere to be carried out. The European Numerical Simulation Network (ENSN) aims at providing a theoretical support to the mission. We describe the achievements of the ENSN during its first period of activity 1991–1994, during which the network was funded by the European Union. In particular, the ENSN has set up (i) thematic Working Groups on the prime scientific goals of the mission, (ii) a code development Working Group to develop numerical simulation codes specifically adapted to studying magnetospheric boundaries and the corresponding scale mixing, and (iii) software models of Cluster instruments to test in a numerical simulation what the set of four instruments will measure.     

MTI data clustering and formation recognition

Image exploitation technology approaches have generally focused on the detection and spatial analysis of stationary groups of objects on the ground using various sensors. While spatial arrangement is clearly necessary in analyzing military formations, it is usually not sufficient. Typically the arrangement must be examined within some context in order to interpret a pattern of deployment. For moving objects the spatial arrangement of the group relative to the direction of motion is key to recognizing the formation. By examining ground moving target indicator (MTI) radar data over time, motion can be inferred and used to establish a context for interpreting the spatial arrangement of the data. New techniques that exploit the multitemporal nature of MTI data are described. The first is a space-time clustering technique that locates compact groups of objects that persist in time. The technique Is an application of Marr and Hildreth's edge detection methodology to the dual problem of region segmentation, or more accurately, volumetric segmentation of space-time. The second technique is based on the use of the Hough transform for recognizing moving formations such as columns, wedges, and lines abreast by analyzing the shape of clustered MTI detections (specifically the orientation of linear arrangements within the group) with respect to their direction of motion. Preliminary results from simulated MTI data sets are presented     

Intrinsic time scale for reconnection on the dayside magnetopause

Recently much attention has been focused on the transient behavior of the magnetopause in response to pressure pulses and southward fluctuations of the interplanetary magnetic field. We examine the motion of the magnetopause behind the foreshock and conclude that this motion is affected by foreshock pressure variations but not by fluctuations in the direction of the magnetic field. Neither magnetopause erosion nor flux transfer event occurrence is controlled by the foreshock. On the contrary, flux transfer events occur at times of steady IMF and thier quasi-periodic behavior is controlled by the magnetopause or the magnetosphere and is not driven by the external boundary conditions. Since flux transfer events are clearly due to reconnection, this observation implies that the IMF must be southward some time perhaps as long as 7 minutes before flux transfer begins.     

EUV emission from normal galaxies

Using data from the Wide Field Camera EUV all-sky survey, we have established upper limits to the EUV flux from a sample of 30 bright, nearby, non-active spiral galaxies. These galaxies were chosen to be those most likely to be detected in the EUV on the basis of (i) low interstellar absorption within our own galaxy, (ii) brightness in other wavebands, (iii) high star formation activity, and (iv) proximity. The derived EUV upper limits are restrictive, and establish for the first time that the EUV flux escaping from galaxies does not constitute a major component of their bolometric luminosity, and in particular that it cannot be the sink for the energy injected into the interstellar medium by supernova explosions, as had been suggested following the failure to detect this power in the X-ray band.     

The INFRARED SPACE OBSERVATORY (ISO)

The INFRARED SPACE OBSERVATORY (ISO), a project of the European Space Agency (ESA), will make various astronomical observations in the wavelength range of 2 to 200 μm. Two-thirds of its observing time will be available to guest observers via the traditional route of proposal submission. ISO will be launched in 1995 by an Ariane 4 from Kourou (Guyana) and be brought into a very elongated orbit with a 24hr period of which 16 hr can be used for astronomical observations. The payload module is essentially a large cryostat with a tank that will be filled with over 2000 litres of superfluid helium to keep the instruments cold (2 to 4 K) during an expected lifetime of 18 months. Inside the dewar, there is a Ritchey-Chretien telescope with a primary mirror of diameter 60cm. Four instruments, each provided by a different PI consortium, share the ISO focal plane. These instruments are : a photometer, a camera and two spectrometers — one for the wavelength range 2–45 μm and the other for 45–180 μm.     

Large lithium ion batteries for aerospace and aircraft applications

Eagle-Picher Energy Products (EPEP) has been manufacturing and testing large lithium ion cells (up to 100-Ah) for several years. Recently, work has focused on the testing of different chemistries at variable temperatures and designing and fabricating 100-Ah cylindrical cells. For the aircraft application the largest concern is irreversible capacity loss at elevated temperatures (70°C). In contrast, for the aerospace application shelf-life and cycle life are critical. EPEP has found that the major contributor to the loss in low temperature performance due to high temperature testing, was the positive electrode. EPEP discuss recent results of variable temperature cycling and 100-Ah cell performance