Classification of modern and old Río Tinto sedimentary deposits through the biomolecular record using a life marker biochip: implications for detecting life on Mars

The particular mineralogy formed in the acidic conditions of the Río Tinto has proven to be a first-order analogue for the acid-sulfate aqueous environments of Mars. Therefore, studies about the formation and preservation of biosignatures in the Río Tinto will provide insights into equivalent processes on Mars. We characterized the biomolecular patterns recorded in samples of modern and old fluvial sediments along a segment of the river by means of an antibody microarray containing more than 200 antibodies (LDCHIP200, for Life Detector Chip) against whole microorganisms, universal biomolecules, or environmental extracts. Samples containing 0.3-0.5?g of solid material were automatically analyzed in situ by the Signs Of LIfe Detector instrument (SOLID2), and the results were corroborated by extensive analysis in the laboratory. Positive antigen-antibody reactions indicated the presence of microbial strains or high-molecular-weight biopolymers that originated from them. The LDCHIP200 results were quantified and subjected to a multivariate analysis for immunoprofiling. We associated similar immunopatterns, and biomolecular markers, to samples with similar sedimentary age. Phyllosilicate-rich samples from modern fluvial sediments gave strong positive reactions with antibodies against bacteria of the genus Acidithiobacillus and against biochemical extracts from Río Tinto sediments and biofilms. These samples contained high amounts of sugars (mostly polysaccharides) with monosaccharides like glucose, rhamnose, fucose, and so on. By contrast, the older deposits, which are a mix of clastic sands and evaporites, showed only a few positives with LDCHIP200, consistent with lower protein and sugar content. We conclude that LDCHIP200 results can establish a correlation between microenvironments, diagenetic stages, and age with the biomarker profile associated with a sample. Our results would help in the search for putative martian biomarkers in acidic deposits with similar diagenetic maturity. Our LDCHIP200 and SOLID-like instruments may be excellent tools for the search for molecular biomarkers on Mars or other planets.     

Coherent optical array receiver for PPM signals under atmospheric turbulence

Adaptive combining of experimentally obtained heterodyned pulse position modulated (PPM) signals with pulse-to-pulse coherence, in the presence of simulated spatial distortions resembling atmospheric turbulence, is demonstrated. The adaptively combined PPM signals are phased up via an least-mean-square algorithm suitably optimized to operate with PPM in the presence of additive shot noise. A convergence analysis of the algorithm is presented, and results with both computer simulated and experimentally obtained PPM signals are analyzed.     

Development of standards for aeronautical satellite navigation system

One of the work objectives of the International Civil Aviation Organisation (ICAO) is the development of the standards and procedures necessary to support transition to the CNS/ATM systems, which include Global Navigation Satellite System (GNSS). The Global Navigation Satellite System Panel (GNSSP) was established by the ICAO Air Navigation Commission in 1993 with the basic objective to develop ICAO standards and recommended practices (SARPs) and guidance material as required to support aeronautical GNSS applications world-wide. The first package of GNSS SARPs was adopted and published by ICAO in 2001, and further work is under way to introduce new satellite constellations and system elements in an evolutionary fashion.     

Modeling the solar cosmic ray event of 13 December 2006 using ground level neutron monitor data

In order to understand the physics under extreme solar conditions such as those producing ground level enhancements of solar cosmic rays, it is important to use accurate and reliable models. The NM-BANGLE Model is a new cosmic ray model which couples primary solar cosmic rays at the top of the Earth’s atmosphere with the secondary ones detected at ground level by neutron monitors during GLEs. This model calculates the evolution of several GLE parameters such as the solar cosmic ray spectrum, anisotropy and particle flux distribution, revealing crucial information on the energetic particle propagation and distribution. The total output of the NM-BANGLE Model is a multi-dimensional GLE picture that gives an important contribution to revealing the characteristics of solar energetic particle events recorded at ground level. In this work, the results of the NM-BANGLE Model application to the recent GLE of 13 December 2006 are presented and discussed. Moreover, a comparison with the extreme event of 20 January 2005 (GLE69) has been realized.     

FM Telemetry for Multiple Force Measurements on Free-Flying Models

The extension of the FM telemetry technique to provide simultaneous measurements of four forces acting on a free-flight aerodynamic model is reported. Developments and procedures required to achieve the accuracy, linearity, and stability necessary for obtaining dynamic stability coefficients are described. System accuracy is believed to be better than 3 percent. Free-flight data, presented in the form of aerodynamic stability derivatives, are in good agreement with measurements taken by conventional methods under the same aerodynamic conditions.     

Interactive database of cosmic ray anisotropy (DB-A10)

The worldwide neutron monitor network is a unique tool for obtaining with high accuracy the information on density variations, energy spectrum and anisotropy of comic rays at the Earth, outside its atmosphere and magnetosphere. These hourly averaged parameters were obtained over the whole period of cosmic ray monitoring by the ground level neutron monitor network (from 1957 till present) and are collected within the MySQL database. The Internet-project has developed for free access and supplying of cosmic ray density and anisotropy data in different formats.     

Russian ground-level detectors of cosmic ray observations as a part of the world wide network: History and development

The history of creation and development of the network of cosmic ray stations in USSR-Russia goes back to the difficult years of the Second War (1944–1945). The Russian neutron monitor network continuously operates at the present time, having developed from a mechanical means of registration into a modern electronic system for the collection and processing of data with the results presented in the Internet in real time. Along with the improvement of the equipment and different methods of data processing, strong scientific groups, and even Institutes have grown up at a number of stations. They carry out scientific investigations on the basis of neutron monitor network data and provide the conditions for operative and real time exchange of data.     

Biochemical and hemodynamic changes in normal subjects during acute and rigorous bed rest and ambulation

Rigorous bed rest (RBR) induces significant biochemical and circulatory changes. However, little is known about acute rigorous bed rest (ARBR). Measuring biochemical and circulatory variables during ARBR and RBR the aim of this study was to establish the significance of ARBR effect. Studies were done during 3 days of a pre-bed rest (BR) period and during 7 days of ARBR and RBR period. Thirty normal male individuals aged, 24.1 +/- 6.3 years were chosen as subjects. They were divided equally into three groups: 10 subjects placed under active control conditions served as unrestricted ambulatory control subjects (UACS), 10 subjects submitted to an acute rigorous bed rest served as acute rigorous bed rested subjects (ARBRS) and 10 subjects submitted to a rigorous bed rest served as rigorous bed rested subjects (RBRS). The UACS were maintained under an average running distance of 9.7 km day-1. For the ARBR effect simulation, ARBRS were submitted abruptly to BR for 7 days. They did not have any prior knowledge of the exact date and time when they would be asked to confine to RBR. For the RBR effect simulation, RBRS were subjected to BR for 7 days on a predetermined date and time known to them right away from the start of the study. Plasma renin activity (PRA), plasma cortisol (PC), plasma aldosterone (PA), plasma and urinary sodium (Na) and potassium (K) levels, heart rate (HR), cardiac output (CO), and arterial blood pressure (ABP) increased significantly, and urinary aldosterone (UA), stroke volume (SV) and plasma volume (PV) decreased significantly (p<0.05) in ARBRS and RBRS as compared with their pre-BR values and the values in UACS. Electrolyte, hormonal and hemodynamic responses were significantly (p<0.05) greater and occurred significantly faster (p<0.05) during ARBR than RBR. Parameters change insignificantly (p>0.05) in UACS compared with pre-BR control values. It was concluded that, the more abruptly muscular activity is restricted in experimental subjects while they are very active, the greater hemodynamic and biochemical change there is and probably in individuals whose muscular activity is abruptly terminated after an accident or sudden illness.     

Different space weather effects in anomalies of the high and low orbital satellites

Preliminary results of the EU INTAS Project 00810, which aims to improve the methods of safeguarding satellites in the Earth’s magnetosphere from the negative effects of the space environment, are presented. Anomaly data from the “Kosmos” series satellites in the period 1971–1999 are combined in one database, together with similar information on other spacecraft. This database contains, beyond the anomaly information, various characteristics of the space weather: geomagnetic activity indices (Ap, AE and Dst), fluxes and fluences of electrons and protons at different energies, high energy cosmic ray variations and other solar, interplanetary and solar wind data. A comparative analysis of the distribution of each of these parameters relative to satellite anomalies was carried out for the total number of anomalies (about 6000 events), and separately for high (5000 events) and low (about 800 events) altitude orbit satellites. No relation was found between low and high altitude satellite anomalies. Daily numbers of satellite anomalies, averaged by a superposed epoch method around sudden storm commencements and proton event onsets for high (>1500 km) and low (<1500 km) altitude orbits revealed a big difference in a behavior. Satellites were divided on several groups according to the orbital characteristics (altitude and inclination). The relation of satellite anomalies to the environmental parameters was found to be different for various orbits that should be taken into account under developing of the anomaly frequency models.     

Gypsum-permineralized microfossils and their relevance to the search for life on Mars

Abstract Orbital and in situ analyses establish that aerially extensive deposits of evaporitic sulfates, including gypsum, are present on the surface of Mars. Although comparable gypsiferous sediments on Earth have been largely ignored by paleontologists, we here report the finding of diverse fossil microscopic organisms permineralized in bottom-nucleated gypsums of seven deposits: two from the Permian (～260?Ma) of New Mexico, USA; one from the Miocene (～6?Ma) of Italy; and four from Recent lacustrine and saltern deposits of Australia, Mexico, and Peru. In addition to presenting the first report of the widespread occurrence of microscopic fossils in bottom-nucleated primary gypsum, we show the striking morphological similarity of the majority of the benthic filamentous fossils of these units to the microorganisms of a modern sulfuretum biocoenose. Based on such similarity, in morphology as well as habitat, these findings suggest that anaerobic sulfur-metabolizing microbial assemblages have changed relatively little over hundreds of millions of years. Their discovery as fossilized components of the seven gypsiferous units reported suggests that primary bottom-nucleated gypsum represents a promising target in the search for evidence of past life on Mars. Key Words: Confocal laser scanning microscopy-Gypsum fossils-Mars sample return missions-Raman spectroscopy-Sample Analysis at Mars (SAM) instrument-Sulfuretum. Astrobiology 12, 619-633.