Supercooled water brines within permafrost-an unknown ecological niche for microorganisms: a model for astrobiology

This study describes brine lenses (cryopegs) found in Siberian permafrost derived from ancient marine sediment layers of the Arctic Ocean. The cryopegs were formed and isolated from sediment ~100,000-120,000 years ago. They remain liquid at the in situ temperature of -10 degrees C as a result of their high salt content (170-300 g/L). [(14)C] Glucose is taken up by the cryopeg biomass at -15 degrees C, indicating microbial metabolism at low temperatures in this habitat. Furthermore, aerobic, anaerobic heterotrophs, sulfate reducers, acetogens, and methanogens were detected by most probable number analysis. Two psychrophilic microbes were isolated from the cryopegs, a Clostridium and a Psychrobacter. The closest relatives of each were previously isolated from Antarctica. The cryopeg econiche might serve as a model for extraterrestrial life, and hence is of particular interest to astrobiology.     

Magnetotactic bacteria on Earth and on Mars

Continued interest in the possibility of evidence for life in the ALH84001 Martian meteorite has focused on the magnetite crystals. This review is structured around three related questions: is the magnetite in ALH84001 of biological or non-biological origin, or a mixture of both? does magnetite on Earth provide insight to the plausibility of biogenic magnetite on Mars? could magnetotaxis have developed on Mars? There are credible arguments for both the biological and non-biological origin of the magnetite in ALH84001, and we suggest that more studies of ALH84001, extensive laboratory simulations of non-biological magnetite formation, as well as further studies of magnetotactic bacteria on Earth will be required to further address this question. Magnetite grains produced by bacteria could provide one of the few inorganic traces of past bacterial life on Mars that could be recovered from surface soils and sediments. If there was biogenic magnetite on Mars in sufficient abundance to leave fossil remains in the volcanic rocks of ALH84001, then it is likely that better-preserved magnetite will be found in sedimentary deposits on Mars. Deposits in ancient lakebeds could contain well-preserved chains of magnetite clearly indicating a biogenic origin.     

Quasistatic Microaccelerations onboard the Foton Satellite during Its Flight at High-Altitude Orbits

The level of quasistatic microaccelerations onboard the Foton-M satellite is predicted for its flights in two orbits: the planned orbit with the altitudes in perigee h = 262 km and in apogee h = 304 km and the orbit with h = 262 km and h = 350 km. The prediction is based on mathematical simulation of the satellite motion with respect to its center of mass under the action of gravitational and aerodynamic moments. The model is represented by the system of equations of the satellite rotational motion. Parameters of this system are chosen from the condition of coincidence of the motion of preceding Foton satellites (h 220 km and h 400 km) calculated using this model with the results of determination of actual rotational motion of the Foton-11 and Foton-12 satellites. With the help of the model thus calibrated, a calculation is made of the rotational motion of the Foton-M satellite and of the quasistatic microaccelerations onboard it. As is shown by the results of simulation, the use of the first and the second orbits will result in reductions of microaccelerations by 30% and 60%, respectively.     

Evaluation of bleomycin-induced chromosome aberrations under simulated microgravity conditions in human lymphocytes using "FISH" techniques

In the present investigation we report the effects of simulated microgravity conditions (clinostat) on the induction of chromosomal aberrations in human lymphocytes in vitro by (R) Bleomycin. Chromosomal aberrations have been analysed by means of fluorescent in situ hybridisation (FISH) and chromosome-specific composite DNA probes (chromosome painting). The results obtained show that, under simulated microgravity conditions, the levels of both symmetrical and asymmetrical (dicentrics, rings), the number of cells bearing "complex" aberrations and hence the total numbers of aberrations were significantly elevated at any of the dose-levels assayed, compared to the parallel treatments performed as 1g control ("ground"). Furthermore, the ratio symmetrical:asymmetrical translocations was markedly elevated under simulated microgravity conditions, compared to the findings usually observed under "normal" 1g conditions. On these bases, we are much inclined to believe that simulated microgravity, rather than limiting the resealing of DNA double strand breaks (DSB's) induced by genotoxic agents is influencing in terms of enhancement the misrejoining of DSB's which is actually responsible for the fixation of the original lesions to DNA into chromosomal aberrations. In addition, the possible different misrepair processes leading to the formation of symmetrical and asymmetrical translocations might be differentially influenced by microgravity being the symmetrical translocations significantly more represented.     

Peculiarities of the Orbital Motion of Submicron Particles in the Earth's Plasmasphere

On the basis of numerical calculations of trajectories, the peculiarities of motion of submicron-sized particles in the Earth's plasmasphere are investigated. The most important result of these investigations is the found possibility of long-term residence of a microparticle in the Earth's vicinity. This effect is a result of the interaction of the electric charge, induced on a microparticle, with the magnetic field of the Earth. It is shown that the effect of microparticle capture by the Earth's magnetic field takes place in the case when the microparticles having a dimension of about 10^–2 m and made of a material having high yield of photoemission are injected into the plasmasphere at altitudes of about several thousand kilometers and also in the case when the microparticles with a dimension of about 10^–3 m and made of a material having low yield of photoemission are injected into the plasmasphere at altitudes of about 15000–20000 km and are moving close to the equatorial plane.     

Human interactions in space: results from Shuttle/Mir.

Background: Anecdotal reports from space and results from simulation studies on Earth have suggested that space crewmembers may experience decrements in their interpersonal environment over time and may displace tension and dysphoria to mission control personnel. Methods: To evaluate these issues, we studied 5 American astronauts, 8 Russian cosmonauts, and 42 American and 16 Russian mission control personnel who participated in the Shuttle/Mir space program. Subjects completed questions from subscales of the Profile of Mood States, the Group Environment Scale, and the Work Environment Scale on a weekly basis before, during, and after the missions. Results: Among the crewmembers, there was little evidence for significant time effects based on triphasic (U-shaped) or linear models for the 21 subscales tested, although the presence of an initial novelty effect that declined over time was found in three subscales for the astronauts. Compared with work groups on Earth, the crewmembers reported less dysphoria and perceived their crew environment as more constraining, cohesive, and guided by leadership. There was no change in ratings of mood and interpersonal environment before, during, and after the missions. Conclusions: There was little support for the presence of a moderate to strong time effect that influenced the space crews. Crewmembers perceived their work environment differently from people on Earth, and they demonstrated equanimity in mood and group perceptions, both in space and on the ground. Grant numbers: NAS9-19411.     

Spatial factors and muscle spindle input influence the generation of neuromuscular responses to stimulation of the human foot

Removal of the mechanical pressure gradient on the soles leads to physiological adaptations that ultimately result in neuromotor degradation during spaceflight. We propose that mechanical stimulation of the soles serves to partially restore the afference associated with bipedal loading and assists in attenuating the negative neuromotor consequences of spaceflight. A dynamic foot stimulus device was used to stimulate the soles in a variety of conditions with different stimulation locations, stimulation patterns and muscle spindle input. Surface electromyography revealed the lateral side of the sole elicited the greatest neuromuscular response in ankle musculature, followed by the medial side, then the heel. These responses were modified by preceding stimulation. Neuromuscular responses were also influenced by the level of muscle spindle input. These results provide important information that can be used to guide the development of a “passive” countermeasure that relies on sole stimulation and can supplement existing exercise protocols during spaceflight.     

The space life sciences strategy for the 21st century

In the past, space life sciences has focused on gaining an understanding of physiological tolerance to spaceflight, but, for the last 10 years, the focus has evolved to include issues relevant to extended duration missions. In the 21st century, NASA's long-term strategy for the exploration of the solar system will combine the assurance of human health and performance for long periods in space with investigations aimed at searching for traces of life on other planets and acquiring fundamental scientific knowledge of life processes. Implementation of this strategy will involve a variety of disciplines including radiation health, life support, human factors, space physiology and countermeasures, medical care, environmental health, and exobiology. It will use both ground-based and flight research opportunities such as those found in current on-going programs, on Spacelab and unmanned biosatellite flights, and during Space Station Freedom missions.     

Some Matched Filter Configurations for Infrared Systems

An analysis of the output of three alternative matched filter configurations in an infrared scanning system model is presented. The sensor is corrupted by thermal noise, generation-recombination noise, photon noise, and modulation noise, the latter providing an extreme discoloration in the signal passband. Expressions for the signal voltage density spectrum, signal pulse shape, noise power spectrum, and average noise power at the matched filter output are derived where the integral evaluations attendant to these derivations do not appear elsewhere in the literature. The paper also provides graphical displays of the signal-to-noise power ratio at the filter output versus various system parameters, noise power spectrum out of the matched filter versus ?, and the signal pulse shape out of the filter versus time. Also included are discussions of practically realizable approximations to the matched filters and curve fitting techniques for the signal pulse shape function.     

Optimum Position Estimation Using Multiple-Frequency Omega

The maximum-likelihood estimator of the line of position (LOP) resulting from K frequencies received from each of two Omega stations for high SNR is derived. The estimator takes advantage of interfrequency correlation of phase uncertainties by modeling them as resulting from phase velocity uncertainties which are jointly Gaussian with known covariance. The utilization of Omega for search and rescue systems is examined. For this application, the result of increasing the size of the unambiguous lanes of Omega by addition of a fourth frequency to the present three-frequency format is examined. The results of Monte Carlo simulation, performed for several easily implemented fourth frequencies, are presented. The simulation results illustrate the extent to which lane errors can be expected as a function of various fourth frequencies, phase velocity uncertainties, and SNR.