Radiation biodosimetry: applications for spaceflight.

The multiparametric dosimetry system that we are developing for medical radiological defense applications could be adapted for spaceflight environments. The system complements the internationally accepted personnel dosimeters and cytogenetic analysis of chromosome aberrations, considered the best means of documenting radiation doses for health records. Our system consists of a portable hematology analyzer, molecular biodosimetry using nucleic acid and antigen-based diagnostic equipment, and a dose assessment management software application. A dry-capillary tube reagent-based centrifuge blood cell counter (QBC Autoread Plus, Becton [correction of Beckon] Dickinson Bioscience) measures peripheral blood lymphocytes and monocytes, which could determine radiation dose based on the kinetics of blood cell depletion. Molecular biomarkers for ionizing radiation exposure (gene expression changes, blood proteins) can be measured in real time using such diagnostic detection technologies as miniaturized nucleic acid sequences and antigen-based biosensors, but they require validation of dose-dependent targets and development of optimized protocols and analysis systems. The Biodosimetry Assessment Tool, a software application, calculates radiation dose based on a patient's physical signs and symptoms and blood cell count analysis. It also annotates location of personnel dosimeters, displays a summary of a patient's dosimetric information to healthcare professionals, and archives the data for further use. These radiation assessment diagnostic technologies can have dual-use applications supporting general medical-related care.     

Competition of the natural and manmade biotic cycles in the closed aquatic system.

This study addresses competition between the Paramecium bursaria and zoochlorella-endosymbiosis and the infusoria Paramecium caudatum in a closed aquatic system. The system is a natural model of a simple biotic cycle. P. bursaria consumes glucose and oxygen released by its zoochlorella and releases nitrogenous compounds and carbon dioxide necessary for algal photosynthesis. P. caudatum was fed on bacteria. It was shown that the infusoria P. bursaria united in one cycle with Chlorella had a higher competitive ability than P. caudatum. With any initial percentage of the infusoria in the mixed culture, the end portion of P. bursaria reached 90-99%, which was significantly higher than the end potion of the P. caudatum population. It is assumed that the sustenance expenditures of P. caudatum were greater than those of the endosymbiotic paramecium, i.e. the closing of the components into a biotic cycle leads to a decrease in sustenance expenditures.     

National paper of the Russian Federation

Despite its straightened circumstances and doubts about how much political support it receives, Russia's space programme continues — and continues to plan for new activities and applications. This edited version of the National Paper of the Russian Federation, presented at Unispace III in July 1999, details some of the country's major achievements in space in recent years and sets out the main areas it intends to pursue for the early twentieth-first century. While emphasis is placed on communications, remote sensing, navigation and space science, the commitment to manned flight remains.     

System identification of dynamic closed-loop control of total peripheral resistance by arterial and cardiopulmonary baroreceptors.

Prolonged exposure to microgravity in space flight missions (days) impairs the mechanisms responsible for defense of arterial blood pressure (ABP) and cardiac output (CO) against orthostatic stress in the post-flight period. The mechanisms responsible for the observed orthostatic intolerance are not yet completely understood. Additionally, effective counter measures to attenuate this pathophysiological response are not available. The aim of this study was to investigate the ability of our proposed system identification method to predict closed-loop dynamic changes in TPR induced by changes in mean arterial pressure (MAP) and right atrial pressure (RAP). For this purpose we designed and employed a novel experimental animal model for the examination of arterial and cardiopulmonary baroreceptors in the dynamic closed-loop control of total peripheral resistance (TPR), and applied system identification to the analysis of beat-to-beat fluctuations in the measured signals. Grant numbers: NAG5-4989.     

Initial closed operation of the CELSS Test Facility Engineering Development Unit.

As part of the NASA Advanced Life Support Flight Program, a Controlled Ecological Life Support System (CELSS) Test Facility Engineering Development Unit has been constructed and is undergoing initial operational testing at NASA Ames Research Center. The Engineering Development Unit (EDU) is a tightly closed, stringently controlled, ground-based testbed which provides a broad range of environmental conditions under which a variety of CELSS higher plant crops can be grown. Although the EDU was developed primarily to provide near-term engineering data and a realistic determination of the subsystem and system requirements necessary for the fabrication of a comparable flight unit, the EDU has also provided a means to evaluate plant crop productivity and physiology under controlled conditions. This paper describes the initial closed operational testing of the EDU, with emphasis on the hardware performance capabilities. Measured performance data during a 28-day closed operation period are compared with the specified functional requirements, and an example of inferring crop growth parameters from the test data is presented. Plans for future science and technology testing are also discussed.     

Population dynamics of transgenic microorganisms in the different microecosystem conditions.

The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap (r) Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human alpha 2-interferon Km (r) Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. Grant numbers: 99-04-96017, 25, 00-07-9011.     

The Low-Latitude Boundary Layer at the Flanks of the Magnetopause

This is a brief overview on what we know and do not know about the low-latitude boundary layer (LLBL) at the flanks of the magnetotail. On the basis of recent observations, simulations and theories we conclude that reconnection is the dominant process in generating the LLBL and its structure probably even under northward IMF conditions. Part of the LLBL always seems to be on open field lines. Possibly the LLBL possesses a double structure with its outer part open and inner part closed. Anomalous diffusive processes cannot sustain the LLBL but provide sufficient diffusivity for reconnection. Strong diffusion is only expected in narrow localized regions and can make the transition to superdiffusion. Kelvin-Helmholtz instability (KHI) is favoured for northward IMF, producing vortices at the tail flanks. Its contribution to efficient mass transport still remains questionable. Coupling of the LLBL to the ionosphere can strongly affect the internal structure of the LLBL, causing turbulent eddies and detachments of plasma blobs as also field-aligned currents and electron heating. The structure and dynamics of the LLBL are affected by field-aligned electric potentials that decouple the LLBL from the ionosphere. Non-ideal coupling simulations suggest that the dusk flank is decoupled, favouring KHI, while the dawn flank is dominated by currents and turbulence.     

Moving target imaging algorithm for SAR data

A digital processing algorithm for fine-resolution imaging of synthetic aperture radar (SAR) moving targets is described. The targets may have any translational and rotational motion components relative to the data collection platform. The algorithm requires the presence of up to three prominent points in the image of the target; the signals from these points provide estimates of the unknown target motion parameters. Phase compensation and data formatting based on these estimates eliminate motion-induced phase errors. This algorithm has been implemented on a VAX computer and used to process both simulated and real SAR data of moving targets. Results obtained using the simulated data are presented