Changes in plant medium composition after a spaceflight experiment: Potassium levels are of special interest

We present results on the analysis of 100 mL medium samples extracted from sterilized foam (Smithers-Oasis, Kent OH) used to support the growth of a representative dicotyledon (Haplopappus gracilis) and a representative monocotyledon (Hemerocallis cv Autumn Blaze) in NASA’s Plant Growth Unit (PGU) during a 5-day Space Shuttle flight and ground experiments. At recovery, the media remaining within replicate (n = 5) foam blocks (for both the spaceflight and ground experiments) were extracted under vacuum, filtered and subjected to elemental analyses. A unique aspect of this experiment was that all plants were either aseptically-generated tissue culture propagated plantlets or aseptic seedling clones. The design of the PGU facilitated the maintenance of asepsis throughout the mission (confirmed by post-flight microbial sampling) and thus any possible impact of microorganisms on medium composition was eliminated. Concentration levels of some elements remained the same, while some decreased and others increased. There was a significant two-fold difference between the final concentrations of potassium when the Earth-based and microgravity experiments were contrasted.     

Study of the 27-day variations of the galactic cosmic ray intensity and anisotropy

We demonstrate that the general features of the radial and azimuthal components of the anisotropy of galactic cosmic rays can be studied by the harmonic analysis method using data from an individual neutron monitor with cut off rigidity <5 GV. In particular, we study the characteristics of the 27-day (solar rotation period) variations of the galactic cosmic ray intensity and anisotropy, solar wind velocity, interplanetary magnetic field strength and sunspot number. The amplitudes of the 27-day variations of the galactic cosmic ray anisotropy are greater, and the phases more clearly established, in A > 0 polarity periods than in A < 0 polarity periods at times of minimum solar activity. The phases of the 27-day variations of the galactic cosmic rays intensity and anisotropy are opposite with respect to the similar changes of the solar wind velocity in A > 0 polarity periods. No significant dependence of the amplitude of the 27-day variation of the galactic cosmic ray anisotropy on the tilt angle of the heliospheric neutral sheet is found. Daily epicyclegrams obtained by Chree’s method show that the 27-day variations of the galactic cosmic ray anisotropy during A > 0 polarity periods follow elliptical paths with the major axes oriented approximately along the interplanetary magnetic field. The paths are more irregular during A < 0 polarity periods.     

Evaluation of upper body muscle activity during cardiopulmonary resuscitation performance in simulated microgravity

Performance of efficient single-person cardiopulmonary resuscitation (CPR) is vital to maintain cardiac and cerebral perfusion during the 2–4 min it takes for deployment of advanced life support during a space mission. The aim of the present study was to investigate potential differences in upper body muscle activity during CPR performance at terrestrial gravity (+1Gz) and in simulated microgravity (μG). Muscle activity of the triceps brachii, erector spinae, rectus abdominis and pectoralis major was measured via superficial electromyography in 20 healthy male volunteers. Four sets of 30 external chest compressions (ECCs) were performed on a mannequin. Microgravity was simulated using a body suspension device and harness; the Evetts–Russomano (ER) method was adopted for CPR performance in simulated microgravity. Heart rate and perceived exertion via Borg scores were also measured. While a significantly lower depth of ECCs was observed in simulated microgravity, compared with +1Gz, it was still within the target range of 40–50 mm. There was a 7.7% decrease of the mean (±SEM) ECC depth from 48 ± 0.3 mm at +1Gz, to 44.3 ± 0.5 mm during microgravity simulation (p < 0.001). No significant difference in number or rate of compressions was found between the two conditions. Heart rate displayed a significantly larger increase during CPR in simulated microgravity than at +1Gz, the former presenting a mean (±SEM) of 23.6 ± 2.91 bpm and the latter, 76.6 ± 3.8 bpm (p < 0.001). Borg scores were 70% higher post-microgravity compressions (17 ± 1) than post +1Gz compressions (10 ± 1) (p < 0.001). Intermuscular comparisons showed the triceps brachii to have significantly lower muscle activity than each of the other three tested muscles, in both +1Gz and microgravity. As shown by greater Borg scores and heart rate increases, CPR performance in simulated microgravity is more fatiguing than at +1Gz. Nevertheless, no significant difference in muscle activity between conditions was found, a result that is favourable for astronauts, given the inevitable muscular and cardiovascular deconditioning that occurs during space travel.     

Dependence of the 27-day variation of cosmic rays on the global magnetic field of the Sun

We show that the higher range of the heliolongitudinal asymmetry of the solar wind speed in the positive polarity period (A > 0) than in the negative polarity period (A < 0) is one of the important reasons of the larger amplitudes of the 27-day variation of the galactic cosmic ray (GCR) intensity in the period of 1995–1997 (A > 0) than in 1985–1987 (A < 0). Subsequently, different ranges of the heliolongitudinal asymmetry of the solar wind speed jointly with equally important corresponding drift effect are general causes of the polarity dependence of the amplitudes of the 27-day variation of the GCR intensity. At the same time, we show that the polarity dependence is feeble for the last unusual minimum epoch of solar activity 2007–2009 (A < 0); the amplitude of the 27-day variation of the GCR intensity shows only a tendency of the polarity dependence. We present a three dimensional (3-D) model of the 27-day variation of GCR based on the Parker’s transport equation. In the 3-D model is implemented a longitudinal variation of the solar wind speed reproducing in situ measurements and corresponding divergence-free interplanetary magnetic field (IMF) derived from the Maxwell’s equations. We show that results of the proposed 3-D modeling of the 27-day variation of GCR intensity for different polarities of the solar magnetic cycle are in good agreement with the neutron monitors experimental data. To reach a compatibility of the theoretical modeling with observations for the last minimum epoch of solar activity 2007–2009 (A < 0) a parallel diffusion coefficient was increased by ∼40%.     

Characterizing parameters of Jatropha curcas cell cultures for microgravity studies

Jatropha (Jatropha curcas) is a tropical perennial species identified as a potential biofuel crop. The oil is of excellent quality and it has been successfully tested as biodiesel and in jet fuel mixes. However, studies on breeding and genetic improvement of jatropha are limited. Space offers a unique environment for experiments aiming at the assessment of mutations and differential gene expression of crops and in vitro cultures of plants are convenient for studies of genetic variation as affected by microgravity. However, before microgravity studies can be successfully performed, pre-flight experiments are necessary to characterize plant material and validate flight hardware environmental conditions. Such preliminary studies set the ground for subsequent spaceflight experiments. The objectives of this study were to compare the in vitro growth of cultures from three explant sources (cotyledon, leaf, and stem sections) of three jatropha accessions (Brazil, India, and Tanzania) outside and inside the petriGAP, a modified group activation pack (GAP) flight hardware to fit petri dishes. In vitro jatropha cell cultures were established in petri dishes containing a modified MS medium and maintained in a plant growth chamber at 25 ± 2 °C in the dark. Parameters evaluated were surface area of the explant tissue (A), fresh weight (FW), and dry weight (DW) for a period of 12 weeks. Growth was observed for cultures from all accessions at week 12, including subsequent plantlet regeneration. For all accessions differences in A, FW and DW were observed for inside vs. outside the PetriGAPs. Growth parameters were affected by accession (genotype), explant type, and environment. The type of explant influenced the type of cell growth and subsequent plantlet regeneration capacity. However, overall cell growth showed no abnormalities. The present study demonstrated that jatropha in vitro cell cultures are suitable for growth inside PetriGAPs for a period of 12 weeks. The parameters evaluated in this study provide the basic ground work and pre-flight assessment needed to justify a model for microgravity studies with jatropha in vitro cell cultures. Future studies should focus on results of experiments performed with jatropha in vitro cultures in microgravity.     

Inhibitory effect of simulated microgravity on differentiating preosteoblasts

The bone loss induced by microgravity is partly due to the decrease of mature osteoblasts. In the present study, we employed the random positioning machine (RPM) to simulate microgravity and investigated the acute effects of simulated microgravity on the differentiation of 2T3 preosteoblasts. Following 7 days’ culture under normal (1 g) condition, cells were exposed to simulated microgravity for 24 h. The results showed that 24 h treatment of simulated microgravity significantly decreased alkaline phosphatase (ALP) activity without changing the cell morphology. In addition, the mRNA expressions of osteogenic genes, including runt-related gene 2 (Runx2), osterix, osteocalcin (OC), type I collagen (Col I) and bone morphogenetic protein (BMP), were dramatically downregulated. Moreover, western blot analysis of total extracellular signal-regulated kinase (Erk) and phosphorylated Erk (p-Erk) indicated that p-Erk level, which represents the Erk activation status, was increased. Taken together, our results suggested that acute exposure to simulated microgravity inhibited osteoblast differentiation through modulating the expression of osteogenic genes and the Erk activity. These findings provide new insight for bone loss due to microgravity and unloading.     

Simulated microgravity inhibits cell wall regeneration of Penicillium decumbens protoplasts

This work compares cell wall regeneration from protoplasts of the fungus Penicillium decumbens under rotary culture (simulated microgravity) and stationary cultures. Using an optimized lytic enzyme mixture, protoplasts were successfully released with a yield of 5.3 × 10⁵ cells/mL. Under simulated microgravity conditions, the protoplast regeneration efficiency was 33.8%, lower than 44.9% under stationary conditions. Laser scanning confocal microscopy gave direct evidence for reduced formation of polysaccharides under simulated conditions. Scanning electron microscopy showed the delayed process of cell wall regeneration by simulated microgravity. The delayed regeneration of P. decumbens cell wall under simulated microgravity was likely caused by the inhibition of polysaccharide synthesis. This research contributes to the understanding of how gravitational loads affect morphological and physiological processes of fungi.     

Rigidity spectrum of the 27-day variation of the galactic cosmic ray intensity in different epochs of solar activity

We study the temporal evolution of the power rigidity spectrum of the first (27 days) and the second (14 days) harmonics of the 27-day variation of the galactic cosmic ray intensity measured by neutron monitors in the period of 1965–2002. The rigidity spectrum of these variations can be approximated by a power law. We show the rigidity spectra of the first and the second harmonics of the 27-day variation of the galactic cosmic ray intensity have similar time profiles. These spectra are hard (γ ≈ 0.5 ± 0.1) and soft (γ ≈ 1.1 ± 0.2) during solar maximum and minimum activity, respectively. We ascribe this to the alternation of the sizes of the modulation regions responsible for the 27-day variation of the galactic cosmic ray intensity in different epochs of solar activity. Especially, the average radial sizes of the modulation regions of the 27-day variation and the heliolatitudinal extension of the heliolongitudinal asymmetry are smaller during solar minimum than during solar maximum. We show also, that the temporal changes of the power rigidity spectra of the first and the second harmonics of the 27-day variation of the galactic cosmic ray intensity are in a negative correlation with the changes of the rigidity spectrum of the corresponding 11-year variation.     

27-day variations of the galactic cosmic ray intensity and anisotropy in the minimum of the 23rd solar activity cycle

We study the 27-day variations of the solar wind velocity, galactic cosmic ray (GCR) intensity and anisotropy in the last minimum epoch of solar activity (2007–2009, A < 0). The average amplitude of the 27-day variation of the galactic cosmic ray anisotropy (A27A) in the current minimum epoch of solar activity (2007–2009, A < 0) is lesser than in previous positive polarity period as it is expected from the drift theory. So, polarity dependence rule for the 27-day variation of the GCR anisotropy is fully kept. It is a universal principle for the amplitudes of the 27-day variation of the GCR anisotropy. At the same time, the average amplitude of the 27-day variation of the GCR intensity (A27I) remains at the same level as for previous minimum epoch 1995–1997 (A > 0) showing by the same token an violation of its polarity dependence rule established earlier. We assume that this phenomenon could be generally related with the well established 27-day variation of the solar wind velocity being in anti-correlation with the similar changes of the 27-day variation of the GCR intensity. Generally, a character of the heliolongitudinal asymmetry of spatial large-scale structure of the solar wind velocity (SWV) established in the recent minimum epoch, preferentially pronounces in the behavior of the 27-day variation of the GCR intensity than anisotropy. The formation of the 27-day variation of the GCR anisotropy preferentially takes place in a restricted disk like local vicinity in the helioequatorial region, whilst the 27-day variation of the GCR intensity is formed in the global three dimensional vicinity of the heliosphere.     

Modelling the effects of microgravity on the permeability of air interface respiratory epithelial cell layers

Although it has been suggested that microgravity might affect drug absorption in vivo, drug permeability across epithelial barriers has not yet been investigated in vitro during modelled microgravity. Therefore, a cell culture/diffusion chamber was designed specifically to accommodate epithelial cell layers in a 3D-clinostat and allow epithelial permeability to be measured under microgravity conditions in vitro with minimum alteration to established cell culture techniques. Human respiratory epithelial Calu-3 cell layers were used to model the airway epithelium. Cells grown at an air interface in the diffusion chamber from day 1 or day 5 after seeding on 24-well polyester Transwell cell culture inserts developed a similar transepithelial electrical resistance (TER) to cells cultured in conventional cell culture plates. Confluent Calu-3 layers exposed to modelled microgravity in the 3D-clinostat for up to 48 h maintained their high TER. The permeability of the paracellular marker ¹⁴C-mannitol was unaffected after a 24 h rotation of the cell layers in the 3D-clinostat, but was increased 2-fold after 48 h of modelled microgravity. It was demonstrated that the culture/diffusion chamber developed is suitable for culturing epithelial cell layers and, when subjected to rotation in the 3D-clinostat, will be a valuable in vitro system in which to study the influence of microgravity on epithelial permeability and drug transport.     

Effect of salt stress on growth and physiology in amaranth and lettuce: Implications for bioregenerative life support system

Growing plants can be used to clean waste water in bioregenerative life support system (BLSS). However, NaCl contained in the human urine always restricts plant growth and further reduces the degree of mass cycle closure of the system (i.e. salt stress). This work determined the effect of NaCl stress on physiological characteristics of plants for the life support system. Amaranth (Amaranthus tricolor L. var. Huahong) and leaf lettuce (Lactuca sativa L. var. Luoma) were cultivated at nutrient solutions with different NaCl contents (0, 1000, 5000 and 10,000 ppm, respectively) for 10 to 18 days after planted in the Controlled Ecological Life Support System Experimental Facility in China. Results showed that the two plants have different responses to the salt stress. The amaranth showed higher salt-tolerance with NaCl stress. If NaCl content in the solution is below 5000 ppm, the salt stress effect is insignificant on above-ground biomass output, leaf photosynthesis rate, Fv/Fm, photosynthesis pigment contents, activities of antioxidant enzymes, and inducing lipid peroxidation. On the other hand, the lettuce is sensitive to NaCl which significantly decreases those indices of growth and physiology. Notably, the lettuce remains high productivity of edible biomass in low NaCl stress, although its salt-tolerant limitation is lower than amaranth. Therefore, we recommended that amaranth could be cultivated under a higher NaCl stress condition (<5000 ppm) for NaCl recycle while lettuce should be under a lower NaCl stress (<1000 ppm) for water cleaning in future BLSS.     

One dimensional blood flow in a planetocentric orbit

All life on earth is accustomed to the presence of gravity. When gravity is altered, biological processes can go awry. It is of great importance to ensure safety during a spaceflight. Long term exposure to microgravity can trigger detrimental physiological responses in the human body. Fluid redistribution coupled with fluid loss is one of the effects. In particular, in microgravity blood volume is shifted towards the thorax and head. Sympathetic nervous system-induced vasoconstriction is needed to maintain arterial pressure, while venoconstriction limits venous pooling of blood prevents further reductions in venous return of blood to the heart. In this paper, we modify an existing one dimensional blood flow model with the inclusion of the hydrostatic pressure gradient that further depends on the gravitational field modified by the oblateness and rotation of the Earth. We find that the velocity of the blood flow V_B is inversely proportional to the blood specific volume d, also proportional to the oblateness harmonic coefficient J₂, the angular velocity of the Earth ω_E, and finally proportional to an arbitrary constant c. For c = −0.39073 and ξ_H = −0.5 mmHg, all orbits result to less blood flow velocities than that calculated on the surface of the Earth. From all considered orbits, elliptical polar orbit of eccentricity e = 0.2 exhibit the largest flow velocity V_B = 1.031 m/s, followed by the orbits of inclination i = 45°and 0°. The Earth’s oblateness and its rotation contribute a 0.7% difference to the blood flow velocity.     

Upregulation of erythropoietin receptor in UT-7/EPO cells inhibits simulated microgravity-induced cell apoptosis

Hematopoietic progenitor cell proliferation can be altered in either spaceflight or under simulated microgravity experiments on the ground, however, the underlying mechanism remains unknown. Our previous study showed that exposure of the human erythropoietin (EPO)-dependent leukemia cell line UT-7/EPO to conditions of simulated microgravity significantly inhibited the cellular proliferation rate and induced cell apoptosis. We postulated that the downregulation of the erythropoietin receptor (EPOR) expression in UT-7/EPO cells under simulated microgravity may be a possible reason for microgravity triggered apoptosis. In this paper, a human EPOR gene was transferred into UT-7/EPO cells and the resulting expression of EPOR on the surface of UT-7/EPO cells increased approximately 61% (p < 0.05) as selected by the antibiotic G418. It was also shown through cytometry assays and morphological observations that microgravity-induced apoptosis markedly decreased in these UT-7/EPO–EPOR cells. Thus, we concluded that upregulation of EPOR in UT-7/EPO cells could inhibit the simulated microgravity-induced cell apoptosis in this EPO dependent cell line.     

Tilts and polarity separation in the sunspot groups and active regions at the ascending and descending phases of the cycle 23

The Solar Feature Catalogues for sunspots and active regions measured with SOHO/MDI instrument and Ca II K3 spectroheliograph of the Paris-Meudon Observatory are analyzed with the automated classification technique for sunspot groups and active region polarities. We report the first classification results for daily variations of tilt angles (normal and trigonometric ones) in sunspot groups (SG) and active (AR) regions in the cycle 23. The average normal tilts are presented for every year at the ascending and descending phases of the cycle 23 which are similar to those deduced by other authors for the cycles 19–22. The normal tilts of both the sunspot groups and active regions are shown to increase in the ascending phase and a decrease in the descending phase. Similar to SG and AR areas, the trigonometric tilts are shown to have the noticeable North–South asymmetry with the Southern hemisphere dominant in the selected ascending and descending periods. The normal tilt variations with latitude follow Joy’s law revealing a periodicity along the meridian of about 10° and reaching the maximum of 14° at the latitude of about 32° corresponding to the top of the ‘royal zone’ where the sunspots appear. The variations of polarity separation with a latitude are in an anti-phase with those of the tilts reaching a maximum at the latitude of 35° and showing a small positive separation for the groups/active regions in a vicinity of the average tilts ±40°. The ratio R of the polarity separation to the trigonometric tilt fits the linear function of a latitude φ as R = −0.0213φ − 0.1245 confirming positive separation for the polarities of active regions with the average tilts, or the dominance of activity in the Southern hemisphere activity, for the selected period of observations.     

Planetary wave coupling of the atmosphere–ionosphere system during the Northern winter of 2008/2009

This paper presents the global spatial (latitude and altitude) structure and temporal variability of the ∼23-day ionospheric zonally symmetric (s = 0) planetary wave (PW) seen in the Northern winter of 2008/2009 (October 2008–March 2009). It is shown that these ∼23-day ionospheric oscillations are forced from PWs propagating from below. The COSMIC ionospheric parameters f_oF2 and h_mF2 and electron density at fixed altitudes and the SABER temperatures were utilized in order to define the waves which are present simultaneously in the atmosphere and ionosphere. The long-period PWs from the two data sets have been extracted through the same data analysis method. The similarity between the lower thermospheric ∼23-day (s = 0) temperature PW and its ionospheric electron density response provides valuable and strong experimental evidence for confirming the paradigm of atmosphere–ionosphere coupling.     

Simulated microgravity inhibits the proliferation of K562 erythroleukemia cells but does not result in apoptosis

Astronauts and experimental animals in space develop the anemia of space flight, but the underlying mechanisms are still unclear. In this study, the impact of simulated microgravity on proliferation, cell death, cell cycle progress and cytoskeleton of erythroid progenitor-like K562 leukemia cells was observed. K562 cells were cultured in NASA Rotary Cell Culture System (RCCS) that was used to simulate microgravity (at 15 rpm). After culture for 24 h, 48 h, 72 h, and 96 h, the cell densities cultured in RCCS were only 55.5%, 54.3%, 67.2% and 66.4% of the flask-cultured control cells, respectively. The percentages of trypan blue-stained dead cells and the percentages of apoptotic cells demonstrated no difference between RCCS-cultured cells and flask-cultured cells at every time points (from 12 h to 96 h). Compared with flask-cultured cells, RCCS culture induced an accumulation of cell number at S phase concomitant with a decrease at G0/G1 and G2/M phases at 12 h. But 12 h later (from 24 h to 60 h), the distribution of cell cycle phases in RCCS-cultured cells became no difference compared to flask-cultured cells. Consistent with the changes of cell cycle distribution, the levels of intercellular cyclins in RCCS-cultured cells changed at 12 h, including a decrease in cyclin A, and the increasing in cyclin B, D1 and E, and then (from 24 h to 36 h) began to restore to control levels. After RCCS culture for 12–36 h, the microfilaments showed uneven and clustered distribution, and the microtubules were highly disorganized. These results indicated that RCCS-simulated microgravity could induce a transient inhibition of proliferation, but not result in apoptosis, which could involve in the development of space flight anemia. K562 cells could be a useful model to research the effects of microgravity on differentiation and proliferation of hematopoietic cells.     

Near 13.5-day periodicity in Muon Detector data during late 2001 and early 2002

In this study we perform a continuous Morlet wavelet transform method in time series of secondary cosmic rays and 1 AU interplanetary medium parameters for the interval from October 2001 to October 2002. The near 13.5-day periodicity was obtained during late 2001, and it was remarkable for muon data. Even though some works have pointed out that the main activations of the 13.5 day recurrence in near-Earth solar wind are related, e.g., with the heliosheet crossings or to the occurrence at 1 AU of two high speed streams approximately 180° apart in solar longitude per solar rotation, we aim to show that the period of about half the solar rotation during the end months of 2001 present in muon time series was apparently due to the occurrence of non-recurrent interplanetary disturbances. The interconnections among successive Forbush decreases, recovery phases and gradual muon depressions (associated with corotating interaction regions) seem to play an important role in such 13.5-day periodicity.     

In vitro effects of simulated microgravity on Sertoli cell function

With the advent of space flights questions concerning the effects of microgravity (0×G) on human reproductive physiology have received great attention. The aim of this study was to evaluate the influence of 0×G on Sertoli cells. A Sertoli cell line from mouse testis (42GPA9) was analyzed for cytoskeletal and Sex Hormone Binding Globilin (SHBG) changes by immunohistochemistry, for antioxidant content by RT-PCR and for culture medium lactate concentrations by protein chemistry. Cells were cultured for 6, 24 and 48 h on a three-dimensional Random Positioning Machine (3D-RPM); static controls (1×G) were positioned on the supporting frame. At the end of each experiment, cultured cells were either fixed in paraformaldehyde or lysed and RNA-extracted or used for culture medium lactate measurements as needed. At 0×G, Sertoli cytoskeleton became disorganized, microtubules fragmented and SHBG undetectable already after 24 h, with alterations worsening by 48 h. It was evident that various antioxidant systems appreciably increased during the first 24 h but significantly decreased at 48 h. No changes occurred in the 1×G samples. Initially, 0×G seemed to disturb antioxidant protection strategies allowing the testes to support sperm production, thus generating an aging-like state of oxidative stress. Lactate production at 0×G slightly decreased after 24 h. Further experiments are needed in space to investigate upon steroidogenesis and germ cell differentiation within the testis, to rule out male infertility as a possible consequence, which could be a problem, as life expectancy increases.     

Effects of simulated weightlessness on the intestinal mucosal barrier of rats

This study employed a rat tail-suspension model to investigate the effects of simulated weightlessness on the intestinal mucosal barrier. Twenty-four Wistar rats were randomly divided into control (CON), 14-day tail-suspension (SUS-14d), and 21-day tail-suspension (SUS-21d) groups (n = 8 per group). Expression of occludin and zonula occludins-1 (ZO-1), proteins of the tight junction (TJ), in the intestinal mucosa was measured by immunohistochemical analysis, Western blotting, and mRNA fluorescent quantitation PCR. Plasma concentrations of diamine oxidase (DAO) and d-lactate were determined using an enzymatic spectrophotometric assay. Expression of occludin and ZO-1 was reduced in the SUS-14d and SUS-21d groups as compared to the CON group, with lowest expression observed in the SUS-21d group (P < 0.01). Examination by transmission electron microscopy (TEM) of the jejunal epithelium revealed increased intercellular space, decreased TJ and desmosome densities, and destruction of microvilli in the SUS-14d and SUS-21d groups. Plasma DAO and d-lactate concentrations in the SUS-21d group were higher than those in SUS-14d group and significantly higher than those in the CON group (P < 0.01). In all three groups, the expression of occludin and ZO-1 was found to correlate negatively with DAO (P < 0.01) and d-lactate (P < 0.01) concentrations. It is concluded that simulated weightless results in down-regulation of expression of TJ proteins in the rat intestinal mucosa. Simulated weightlessness is proposed to increase intestinal permeability through damage to the TJ.     

Solar and interplanetary precursors of geomagnetic storms in solar cycle 23

Estimating the magnetic storm effectiveness of solar and associated interplanetary phenomena is of practical importance for space weather modelling and prediction. This article presents results of a qualitative and quantitative analysis of the probable causes of geomagnetic storms during the 11-year period of solar cycle 23: 1996–2006. Potential solar causes of 229 magnetic storms (Dst ? −50 nT) were investigated with a particular focus on halo coronal mass ejections (CMEs). A 5-day time window prior to the storm onset was considered to track backward the Sun’s eruptions of halo CMEs using the SOHO/LASCO CMEs catalogue list. Solar and interplanetary (IP) properties associated with halo CMEs were investigated and correlated to the resulting geomagnetic storms (GMS). In addition, a comparative analysis between full and partial halo CME-driven storms is established. The results obtained show that about 83% of intense storms (Dst ? −100 nT) were associated with halo CMEs. For moderate storms (−100 nT < Dst ? −50 nT), only 54% had halo CME background, while the remaining 46% were assumed to be associated with corotating interaction regions (CIRs) or undetected frontside CMEs. It was observed in this study that intense storms were mostly associated with full halo CMEs, while partial halo CMEs were generally followed by moderate storms. This analysis indicates that up to 86% of intense storms were associated with interplanetary coronal mass ejections (ICMEs) at 1 AU, as compared to moderate storms with only 44% of ICME association. Many other quantitative results are presented in this paper, providing an estimate of solar and IP precursor properties of GMS within an average 11-year solar activity cycle. The results of this study constitute a key step towards improving space weather modelling and prediction.