Critical issues in connection with human planetary missions: protection of and from the environment.

Activities associated with human missions to the Moon or to Mars will interact with the environment in two reciprocal ways: (i) the mission needs to be protected from the natural environmental elements that can be harmful to human health, the equipment or to their operations: (ii) the specific natural environment of the Moon or Mars should be protected so that it retains its value for scientific and other purposes. The following environmental elements need to be considered in order to protect humans and the equipment on the planetary surface: (i) cosmic ionizing radiation, (ii) solar particle events; (iii) solar ultraviolet radiation; (iv) reduced gravity; (v) thin atmosphere; (vi) extremes in temperatures and their fluctuations; (vii) surface dust; (viii) impacts by meteorites and micrometeorites. In order to protect the planetary environment. the requirements for planetary protection as adopted by COSPAR for lander missions need to be revised in view of human presence on the planet. Landers carrying equipment for exobiological investigations require special consideration to reduce contamination by terrestrial microorganisms and organic matter to the Greatest feasible extent. Records of human activities on the planet's surface should be maintained in sufficient detail that future scientific experimenters can determine whether environmental modifications have resulted from explorations. Grant numbers: 14056/99/NL/PA.     

Planetary protection—A microbial ethics approach

Planetary protection policies designed to reduce the cross-transfer of life on spacecraft from one planet to another can either be formulated from the pragmatic instrumental needs of scientific exploration, or from ethical principles. I address planetary protection concerns by starting from a normative ethical framework for the treatment of microorganisms. This argues that they have intrinsic value at the level of the individual through to the level of the community, but at the individual level this ethic can only be theoretical. This approach yields a solution to the problem of the inevitable contamination of Mars by human explorers and suggests that in some instances the local contamination of other planets may be acceptable. An exception would be where this contamination would cause destruction of microbial ecosystems. Within the framework of such an ethic, the term ‘planetary protection’ may be normatively too narrow and ‘planetary preservation’ may better describe the activity of controlling cross-inoculation of planets. I discuss an example of a contamination event that might be ethically acceptable within the framework of ‘preservation’, but would be regarded as unacceptable under current planetary ‘protection’ guidelines.     

A Planetary Park system for Mars

The increasing robotic exploration of Mars and eventual human exploration and settlement of that planet threatens to have a significant environmental impact on scientifically important sites and sites of natural beauty in the form of contamination with micro-organisms and spacecraft parts. By definition, the sites that we might wish to preserve are likely to be those to which robots and humans will be sent. An interventionist step to protect pristine regions of Mars with the formation of a Planetary Park system is proposed. Possible locations for the first seven Planetary Parks are suggested. Landing of unmanned craft in these parks would be forbidden. Although global dust storms can carry microorganisms across the planetary surface, the regulations suggested for these parks will allow for the maximum level of preservation. We also suggest that the Planetary Park system could be applied to the Moon.     

Exchange of meteorites (and life?) between stellar systems

It is now generally accepted that meteorite-size fragments of rock can be ejected from planetary bodies. Numerical studies of the orbital evolution of such planetary ejecta are consistent with the observed cosmic ray exposure times and infall rates of these meteorites. All of these numerical studies agree that a substantial fraction (up to one-third) of the ejecta from any planet in our Solar System is eventually thrown out of the Solar System during encounters with the giant planets Jupiter and Saturn. In this paper I examine the probability that such interstellar meteorites might be captured into a distant solar system and fall onto a terrestrial planet in that system within a given interval of time. The overall conclusion is that it is very unlikely that even a single meteorite originating on a terrestrial planet in our solar system has fallen onto a terrestrial planet in another stellar system, over the entire period of our Solar System's existence. Although viable microorganisms may be readily exchanged between planets in our solar system through the interplanetary transfer of meteoritic material, it seems that the origin of life on Earth must be sought within the confines of the Solar System, not abroad in the galaxy.     

Some ecological mechanisms to generate habitability in planetary subsurface areas by chemolithotrophic communities: the Río Tinto subsurface ecosystem as a model system

Chemolithotrophic communities that colonize subsurface habitats have great relevance for the astrobiological exploration of our Solar System. We hypothesize that the chemical and thermal stabilization of an environment through microbial activity could make a given planetary region habitable. The MARTE project ground-truth drilling campaigns that sampled cryptic subsurface microbial communities in the basement of the Río Tinto headwaters have shown that acidic surficial habitats are the result of the microbial oxidation of pyritic ores. The oxidation process is exothermic and releases heat under both aerobic and anaerobic conditions. These microbial communities can maintain the subsurface habitat temperature through storage heat if the subsurface temperature does not exceed their maximum growth temperature. In the acidic solutions of the Río Tinto, ferric iron acts as an effective buffer for controlling water pH. Under anaerobic conditions, ferric iron is the oxidant used by microbes to decompose pyrite through the production of sulfate, ferrous iron, and protons. The integration between the physical and chemical processes mediated by microorganisms with those driven by the local geology and hydrology have led us to hypothesize that thermal and chemical regulation mechanisms exist in this environment and that these homeostatic mechanisms could play an essential role in creating habitable areas for other types of microorganisms. Therefore, searching for the physicochemical expression of extinct and extant homeostatic mechanisms through physical and chemical anomalies in the Mars crust (i.e., local thermal gradient or high concentration of unusual products such as ferric sulfates precipitated out from acidic solutions produced by hypothetical microbial communities) could be a first step in the search for biological traces of a putative extant or extinct Mars biosphere.     

Planetary Defense From Space: Part 1-Keplerian Theory

A system of two space bases housing missiles is proposed to achieve the Planetary Defense of the Earth against dangerous asteroids and comets. We show that the layout of the Earth–Moon system with the five relevant Lagrangian (or libration) points in space leads naturally to only one, unmistakable location of these two space bases within the sphere of influence of the Earth. These locations are at the two Lagrangian points L1 (in between the Earth and the Moon) and L3 (in the direction opposite to the Moon from the Earth).

We show that placing bases of missiles at L1 and L3 would cause those missiles to deflect the trajectory of asteroids by hitting them orthogonally to their impact trajectory toward the Earth, so as to maximize their deflection. We show that the confocal conics are the best class of trajectories fulfilling this orthogonal deflection requirement.

An additional remark is that the theory developed in this paper is just a beginning of a larger set of future research work. In fact, while in this paper we only develop the Keplerian analytical theory of the Optimal Planetary Defense achievable from the Earth–Moon Lagrangian points L1 and L3, much more sophisticated analytical refinements would be needed to:

1. Take into account many perturbation forces of all kinds acting on both the asteroids and missiles shot from L1 and L3;

2. add more (non-optimal) trajectories of missiles shot from either the Lagrangian points L4 and L5 of the Earth–Moon system or from the surface of the Moon itself;

3. encompass the full range of missiles currently available to the US (and possibly other countries) so as to really see “which asteroids could be diverted by which missiles”, even in the very simplified scheme outlined here.

Outlined for the first time in February 2002, our Confocal Planetary Defense concept is a Keplerian Theory that proved simple enough to catch the attention of scholars, representatives of the US Military and popular writers. These developments could possibly mark the beginning of an “all embracing” mathematical vision of Planetary Defense beyond all learned activities, dramatic movies and unknown military plans covered by secret.     

Geochemical constraints on sources of metabolic energy for chemolithoautotrophy in ultramafic-hosted deep-sea hydrothermal systems

Numerical models are employed to investigate sources of chemical energy for autotrophic microbial metabolism that develop during mixing of oxidized seawater with strongly reduced fluids discharged from ultramafic-hosted hydrothermal systems on the seafloor. Hydrothermal fluids in these systems are highly enriched in H(2) and CH(4) as a result of alteration of ultramafic rocks (serpentinization) in the subsurface. Based on the availability of chemical energy sources, inferences are made about the likely metabolic diversity, relative abundance, and spatial distribution of microorganisms within ultramafic-hosted systems. Metabolic reactions involving H(2) and CH(4), particularly hydrogen oxidation, methanotrophy, sulfate reduction, and methanogenesis, represent the predominant sources of chemical energy during fluid mixing. Owing to chemical gradients that develop from fluid mixing, aerobic metabolisms are likely to predominate in low-temperature environments (<20-30 degrees C), while anaerobes will dominate higher-temperature environments. Overall, aerobic metabolic reactions can supply up to approximately 7 kJ of energy per kilogram of hydrothermal fluid, while anaerobic metabolic reactions can supply about 1 kJ, which is sufficient to support a maximum of approximately 120 mg (dry weight) of primary biomass production by aerobic organisms and approximately 20-30 mg biomass by anaerobes. The results indicate that ultramafic-hosted systems are capable of supplying about twice as much chemical energy as analogous deep-sea hydrothermal systems hosted in basaltic rocks.     

AIT阶段微生物灭菌技术在行星保护任务中的应用与发展

行星保护是每一个开展深空探测活动的国家都应遵守的国际化行为。基于我国深空探测任务中行星保护相关的微生物控制需求,文章首先分析了深空探测器在AIT（总装、集成和测试）阶段负载的微生物主要种类和来源,之后综述NASA和ESA采用的干热灭菌（DHMR）、气相过氧化氢（VHP）等微生物灭菌技术在行星保护任务中的应用与研究现状,最后对加快微生物灭菌技术研究以支持我国未来的行星探测任务提出建议。     

Microbial rock inhabitants survive hypervelocity impacts on Mars-like host planets: first phase of lithopanspermia experimentally tested

The scenario of lithopanspermia describes the viable transport of microorganisms via meteorites. To test the first step of lithopanspermia, i.e., the impact ejection from a planet, systematic shock recovery experiments within a pressure range observed in martian meteorites (5-50 GPa) were performed with dry layers of microorganisms (spores of Bacillus subtilis, cells of the endolithic cyanobacterium Chroococcidiopsis, and thalli and ascocarps of the lichen Xanthoria elegans) sandwiched between gabbro discs (martian analogue rock). Actual shock pressures were determined by refractive index measurements and Raman spectroscopy, and shock temperature profiles were calculated. Pressure-effect curves were constructed for survival of B. subtilis spores and Chroococcidiopsis cells from the number of colony-forming units, and for vitality of the photobiont and mycobiont of Xanthoria elegans from confocal laser scanning microscopy after live/dead staining (FUN-I). A vital launch window for the transport of rock-colonizing microorganisms from a Mars-like planet was inferred, which encompasses shock pressures in the range of 5 to about 40 GPa for the bacterial endospores and the lichens, and a more limited shock pressure range for the cyanobacterium (from 5-10 GPa). The results support concepts of viable impact ejections from Mars-like planets and the possibility of reseeding early Earth after asteroid cataclysms.     

Asteroidal resources for space manufacturing

Earth-approaching asteroids (Apollos and Amors) may be competitive candidates as raw materials for space manufacturing. The total energy per unit mass required to transfer material from some of these bodies to high Earth orbit is comparable to that for lunar material. Recent optical studies suggest ordinary and carbonaceous chondrite compositions for these asteroids, with some containing large quantities of metallic iron and nickel, and others, carbon, hydrogen and nitrogen. Discoveries of several new candidate asteroids over the next few years will allow for a better selection of materials and mission possibilities. Material from one of these asteroids, either in raw or manufactured form, could be returned to the vicinity of the Earth by a solar-powered mass-driver reaction engine. With a requirement of ～60 shuttle flights, and with minimal development costs, an automated mission to a 200-m dia. (10⁷ ton) metal-rich asteroid could be carried out by a mass-driver tug assembled in low Earth orbit using shuttle tankage as reaction mass. Such a tug could, within a few years, move the asteroid into high Earth orbit for the manufacturing of ～ 20 satellite power stations using a portion of the asteroid itself as reaction mass. In the next few years over 100 asteroids in this size range could be discovered, orbits determined and composition types classified using existing earthbased and spaceborne search techniques.     

The PROCESS experiment: an astrochemistry laboratory for solid and gaseous organic samples in low-earth orbit

The PROCESS (PRebiotic Organic ChEmistry on the Space Station) experiment was part of the EXPOSE-E payload outside the European Columbus module of the International Space Station from February 2008 to August 2009. During this interval, organic samples were exposed to space conditions to simulate their evolution in various astrophysical environments. The samples used represent organic species related to the evolution of organic matter on the small bodies of the Solar System (carbonaceous asteroids and comets), the photolysis of methane in the atmosphere of Titan, and the search for organic matter at the surface of Mars. This paper describes the hardware developed for this experiment as well as the results for the glycine solid-phase samples and the gas-phase samples that were used with regard to the atmosphere of Titan. Lessons learned from this experiment are also presented for future low-Earth orbit astrochemistry investigations.     

行星车视觉导航与自主控制进展与展望

以视觉为主的行星车自主地形感知、导航、规划与控制系统是其安全高效探测的重要保障。本文通过对已成功开展和计划中的系列行星车任务导航与控制系统进行汇总，重点梳理了行星车多源地形感知、自主全局和局部导航、自主路径规划与控制等若干关键问题的进展情况，展望了未来自主化、智能化的发展趋势，并结合任务需求构建了行星车视觉导航与自主控制研究框架设想。     

The simulated silicification of bacteria--new clues to the modes and timing of bacterial preservation and implications for the search for extraterrestrial microfossils

Evidence of microbial life on Earth has been found in siliceous rock formations throughout the geological and fossil record. To understand the mechanisms of silicification and thus improve our search patterns for evidence of fossil microbial life in rocks, a series of controlled laboratory experiments were designed to simulate the silicification of microorganisms. The bacterial strains Pseudomonas fluorescens and Desulphovibrio indonensis were exposed to silicifying media. The experiments were designed to determine how exposure time to silicifying solutions and to silicifying solutions of different Si concentration affect the fossilization of microbial biofilms. The silicified biofilms were analyzed using transmission electron microscopy (TEM) in combination with energy-dispersive spectroscopy. Both bacterial species showed evidence of silicification after 24 h in 1,000 ppm silica solution, although D. indonensis was less prone to silicification. The degree of silicification of individual cells of the same sample varied, though such variations decreased with increasing exposure time. High Si concentration resulted in better preservation of cellular detail; the Si concentration was more important than the duration in Si solution. Even though no evidence of amorphous silica precipitation was observed, bacterial cells became permineralized. High-resolution TEM analysis revealed nanometer-sized crystallites characterized by lattice fringe-spacings that match the (10-11) d-spacing of quartz formed within bacterial cell walls after 1 week in 5,000 ppm silica solution. The mechanisms of silicification under controlled laboratory conditions and the implication for silicification in natural environments are discussed, along with the relevance of our findings in the search for early life on Earth and extraterrestrial life.     

Microbial survival rates of Escherichia coli and Deinococcus radiodurans under low temperature, low pressure, and UV-Irradiation conditions, and their relevance to possible Martian life

Viability rates were determined for microbial populations of Escherichia coli and Deinococcus radiodurans under the environmental stresses of low temperature (-35 degrees C), low-pressure conditions (83.3 kPa), and ultraviolet (UV) irradiation (37 W/m(2)). During the stress tests the organisms were suspended in saltwater soil and freshwater soil media, at variable burial depths, and in seawater. Microbial populations of both organisms were most susceptible to dehydration stress associated with low-pressure conditions, and to UV irradiation. However, suspension in a liquid water medium and burial at larger depths (5 cm) improved survival rates markedly. Our results indicate that planetary surfaces that possess little to no atmosphere and have low water availability do not constitute a favorable environment for terrestrial microorganisms.     

Rock surfaces as life indicators: new ways to demonstrate life and traces of former life

Life and its former traces can only be detected from space when they are abundant and exposed to the planetary atmosphere at the moment of investigation by orbiters. Exposed rock surfaces present a multifractal labyrinth of niches for microbial life. Based upon our studies of highly stress-resistant microcolonial fungi of stone monument and desert rock surfaces, we propose that microbial biofilms that develop and become preserved on rock surfaces can be identified remotely by the following characteristics: (1) the existence of spectroscopically identifiable compounds that display unique adsorption, diffraction, and reflection patterns characteristic of biogenerated organic compounds (e.g., chlorophylls, carotenes, melanins, and possibly mycosporines), (2) demonstrably biogenic geomorphological features (e.g., biopitting, biochipping, and bioexfoliation), and (3) biominerals produced in association with biofilms that occupy rock surfaces (e.g., oxalates, forsterite, and special types of carbonates, sulfides, and silicates). Such traces or biosignatures of former life could provide macroscopically visible morphotypes and chemically identifiable products uniquely indicative of life.     

Observations from a 4-year contamination study of a sample depth profile through Martian meteorite Nakhla

Morphological, compositional, and biological evidence indicates the presence of numerous well-developed microbial hyphae structures distributed within four different sample splits of the Nakhla meteorite obtained from the British Museum (allocation BM1913,25). By examining depth profiles of the sample splits over time, morphological changes displayed by the structures were documented, as well as changes in their distribution on the samples, observations that indicate growth, decay, and reproduction of individual microorganisms. Biological staining with DNA-specific molecular dyes followed by epifluorescence microscopy showed that the hyphae structures contain DNA. Our observations demonstrate the potential of microbial interaction with extraterrestrial materials, emphasize the need for rapid investigation of Mars return samples as well as any other returned or impactor-delivered extraterrestrial materials, and suggest the identification of appropriate storage conditions that should be followed immediately after samples retrieved from the field are received by a handling/curation facility. The observations are further relevant in planetary protection considerations as they demonstrate that microorganisms may endure and reproduce in extraterrestrial materials over long (at least 4 years) time spans. The combination of microscopy images coupled with compositional and molecular staining techniques is proposed as a valid method for detection of life forms in martian materials as a first-order assessment. Time-resolved in situ observations further allow observation of possible (bio)dynamics within the system.     

月亮女神探月计划及对我国月球与深空探测的思考

日本月亮女神月球探测器在顺利完成各项探测任务后,于北京时间2009年6月11日受控落月.该探月计划在一箭三星组网探测月球背面重力场、有效载荷创新设计、科研活动组织、成果产出、公众参与和科普宣传等方面有许多亮点,对我国探月工程有重要参考价值.文章综合回顾、分析和评述了月亮女神探月计划的任务、探测器、轨道与飞控、重要事件等...     

Issues in planetary protection: policy, protocol and implementation

Planetary protection is NASA's term for the practice of protecting solar system bodies from Earth life while protecting Earth from life that may be brought back from other solar system bodies. Spacefaring nations will soon begin retrieving samples from Mars and other solar system bodies. For these samples, planetary protection is in order, and measures are already in place to prevent the forward contamination of Mars and other bodies by Earth microbes and the backward contamination of Earth by possible extraterrestrial life. A major goal of planetary protection controls on forward contamination is to preserve the planetary record of natural processes by preventing human-caused microbial introductions.     

Stepping stones toward global space exploration

Several nations are currently engaging in or planning for robotic and human space exploration programs that target the Moon, Mars and near-Earth asteroids. These ambitious plans to build new space infrastructures, transport systems and space probes will require international cooperation if they are to be sustainable and affordable. Partnerships must involve not only established space powers, but also emerging space nations and developing countries; the participation of these new space actors will provide a bottom-up support structure that will aid program continuity, generate more active members in the space community, and increase public awareness of space activities in both developed and developing countries. The integration of many stakeholders into a global space exploration program represents a crucial element securing political and programmatic stability. How can the evolving space community learn to cooperate on a truly international level while engaging emerging space nations and developing countries in a meaningful way? We propose a stepping stone approach toward a global space exploration program, featuring three major elements: (1) an international Earth-based field research program preparing for planetary exploration, (2) enhanced exploitation of the International Space Station (ISS) enabling exploration and (3) a worldwide CubeSat program supporting exploration. An international Earth-based field research program can serve as a truly global exploration testbed that allows both established and new space actors to gain valuable experience by working together to prepare for future planetary exploration missions. Securing greater exploitation of the ISS is a logical step during its prolonged lifetime; ISS experiments, partnerships and legal frameworks are valuable foundations for exploration beyond low Earth orbit. Cooperation involving small, low-cost missions could be a major stride toward exciting and meaningful participation from emerging space nations and developing countries. For each of these three proposed stepping stones, recommendations for coordination mechanisms are presented.