Challenges for coring deep permafrost on Earth and Mars

A scientific drilling expedition to the High Lake region of Nunavut, Canada, was recently completed with the goals of collecting samples and delineating gradients in salinity, gas composition, pH, pe, and microbial abundance in a 400 m thick permafrost zone and accessing the underlying pristine subpermafrost brine. With a triple-barrel wireline tool and the use of stringent quality assurance and quality control (QA/QC) protocols, 200 m of frozen, Archean, mafic volcanic rock was collected from the lower boundary that separates the permafrost layer and subpermafrost saline water. Hot water was used to remove cuttings and prevent the drill rods from freezing in place. No cryopegs were detected during penetration through the permafrost. Coring stopped at the 535 m depth, and the drill water was bailed from the hole while saline water replaced it. Within 24 hours, the borehole iced closed at 125 m depth due to vapor condensation from atmospheric moisture and, initially, warm water leaking through the casing, which blocked further access. Preliminary data suggest that the recovered cores contain viable anaerobic microorganisms that are not contaminants even though isotopic analyses of the saline borehole water suggests that it is a residue of the drilling brine used to remove the ice from the upper, older portion of the borehole. Any proposed coring mission to Mars that seeks to access subpermafrost brine will not only require borehole stability but also a means by which to generate substantial heating along the borehole string to prevent closure of the borehole from condensation of water vapor generated by drilling.     

Suppression of the water ice and snow albedo feedback on planets orbiting red dwarf stars and the subsequent widening of the habitable zone

M stars comprise 80% of main sequence stars, so their planetary systems provide the best chance for finding habitable planets, that is, those with surface liquid water. We have modeled the broadband albedo or reflectivity of water ice and snow for simulated planetary surfaces orbiting two observed red dwarf stars (or M stars), using spectrally resolved data of Earth's cryosphere. The gradual reduction of the albedos of snow and ice at wavelengths greater than 1 μm, combined with M stars emitting a significant fraction of their radiation at these same longer wavelengths, means that the albedos of ice and snow on planets orbiting M stars are much lower than their values on Earth. Our results imply that the ice/snow albedo climate feedback is significantly weaker for planets orbiting M stars than for planets orbiting G-type stars such as the Sun. In addition, planets with significant ice and snow cover will have significantly higher surface temperatures for a given stellar flux if the spectral variation of cryospheric albedo is considered, which in turn implies that the outer edge of the habitable zone around M stars may be 10-30% farther away from the parent star than previously thought.     

Classification of modern and old Río Tinto sedimentary deposits through the biomolecular record using a life marker biochip: implications for detecting life on Mars

The particular mineralogy formed in the acidic conditions of the Río Tinto has proven to be a first-order analogue for the acid-sulfate aqueous environments of Mars. Therefore, studies about the formation and preservation of biosignatures in the Río Tinto will provide insights into equivalent processes on Mars. We characterized the biomolecular patterns recorded in samples of modern and old fluvial sediments along a segment of the river by means of an antibody microarray containing more than 200 antibodies (LDCHIP200, for Life Detector Chip) against whole microorganisms, universal biomolecules, or environmental extracts. Samples containing 0.3-0.5?g of solid material were automatically analyzed in situ by the Signs Of LIfe Detector instrument (SOLID2), and the results were corroborated by extensive analysis in the laboratory. Positive antigen-antibody reactions indicated the presence of microbial strains or high-molecular-weight biopolymers that originated from them. The LDCHIP200 results were quantified and subjected to a multivariate analysis for immunoprofiling. We associated similar immunopatterns, and biomolecular markers, to samples with similar sedimentary age. Phyllosilicate-rich samples from modern fluvial sediments gave strong positive reactions with antibodies against bacteria of the genus Acidithiobacillus and against biochemical extracts from Río Tinto sediments and biofilms. These samples contained high amounts of sugars (mostly polysaccharides) with monosaccharides like glucose, rhamnose, fucose, and so on. By contrast, the older deposits, which are a mix of clastic sands and evaporites, showed only a few positives with LDCHIP200, consistent with lower protein and sugar content. We conclude that LDCHIP200 results can establish a correlation between microenvironments, diagenetic stages, and age with the biomarker profile associated with a sample. Our results would help in the search for putative martian biomarkers in acidic deposits with similar diagenetic maturity. Our LDCHIP200 and SOLID-like instruments may be excellent tools for the search for molecular biomarkers on Mars or other planets.     

LIFE: Life Investigation For Enceladus A Sample Return Mission Concept in Search for Evidence of Life

Abstract Life Investigation For Enceladus (LIFE) presents a low-cost sample return mission to Enceladus, a body with high astrobiological potential. There is ample evidence that liquid water exists under ice coverage in the form of active geysers in the "tiger stripes" area of the southern Enceladus hemisphere. This active plume consists of gas and ice particles and enables the sampling of fresh materials from the interior that may originate from a liquid water source. The particles consist mostly of water ice and are 1-10?μ in diameter. The plume composition shows H(2)O, CO(2), CH(4), NH(3), Ar, and evidence that more complex organic species might be present. Since life on Earth exists whenever liquid water, organics, and energy coexist, understanding the chemical components of the emanating ice particles could indicate whether life is potentially present on Enceladus. The icy worlds of the outer planets are testing grounds for some of the theories for the origin of life on Earth. The LIFE mission concept is envisioned in two parts: first, to orbit Saturn (in order to achieve lower sampling speeds, approaching 2 km/s, and thus enable a softer sample collection impact than Stardust, and to make possible multiple flybys of Enceladus); second, to sample Enceladus' plume, the E ring of Saturn, and the Titan upper atmosphere. With new findings from these samples, NASA could provide detailed chemical and isotopic and, potentially, biological compositional context of the plume. Since the duration of the Enceladus plume is unpredictable, it is imperative that these samples are captured at the earliest flight opportunity. If LIFE is launched before 2019, it could take advantage of a Jupiter gravity assist, which would thus reduce mission lifetimes and launch vehicle costs. The LIFE concept offers science returns comparable to those of a Flagship mission but at the measurably lower sample return costs of a Discovery-class mission. Key Words: Astrobiology-Habitability-Enceladus-Biosignatures. Astrobiology 12, 730-742.     

Europa Lander

A Europa Lander mission has been assigned high priority for the post-2005 time frame in NASA's Space Science Enterprise Strategic Plan. Europa is one of the most scientifically interesting objects in the solar system because of the strong possibility that a liquid water ocean exists underneath its ice-covered surface. The primary scientific goals of the proposed Europa Lander mission are to characterize the surface material from a recent outflow and look for evidence of pre-biotic and possibly biotic chemistry. The baseline mission concept involves landing a single spacecraft on the surface of Europa with the capability to acquire samples of material, perform detailed chemical analysis of the samples, and transmit the results to Earth. This paper provides a discussion of the benefits and status of the key spacecraft and instrument technologies needed to accomplish the science objectives. Also described are variations on the baseline concept including the addition of small auxiliary probes and an experimental ice penetration probe.     

Venus, Mars, and the ices on Mercury and the moon: astrobiological implications and proposed mission designs

Venus and Mars likely had liquid water bodies on their surface early in the Solar System history. The surfaces of Venus and Mars are presently not a suitable habitat for life, but reservoirs of liquid water remain in the atmosphere of Venus and the subsurface of Mars, and with it also the possibility of microbial life. Microbial organisms may have adapted to live in these ecological niches by the evolutionary force of directional selection. Missions to our neighboring planets should therefore be planned to explore these potentially life-containing refuges and return samples for analysis. Sample return missions should also include ice samples from Mercury and the Moon, which may contain information about the biogenic material that catalyzed the early evolution of life on Earth (or elsewhere). To obtain such information, science-driven exploration is necessary through varying degrees of mission operation autonomy. A hierarchical mission design is envisioned that includes spaceborne (orbital), atmosphere (airborne), surface (mobile such as rover and stationary such as lander or sensor), and subsurface (e.g., ground-penetrating radar, drilling, etc.) agents working in concert to allow for sufficient mission safety and redundancy, to perform extensive and challenging reconnaissance, and to lead to a thorough search for evidence of life and habitability.     

Subfreezing activity of microorganisms and the potential habitability of Mars' polar regions

The availability of water-ice at the surface in the Mars polar cap and within the top meter of the high-latitude regolith raises the question of whether liquid water can exist there under some circumstances and possibly support the existence of biota. We examine the minimum temperatures at which liquid water can exist at ice grain-dust grain and ice grain-ice grain contacts, the minimum subfreezing temperatures at which terrestrial organisms can grow or multiply, and the maximum temperatures that can occur in martian high-latitude and polar regions, to see if there is overlap. Liquid water can exist at grain contacts above about -20 degrees C. Measurements of growth in organisms isolated from Siberian permafrost indicate growth at -10 degrees C and metabolism at -20 degrees C. Mars polar and high-latitude temperatures rise above -20 degrees C at obliquities greater than ~40 degrees, and under some conditions rise above 0 degrees C. Thus, the environment in the Mars polar regions has overlapped habitable conditions within relatively recent epochs, and Mars appears to be on the edge of being habitable at present. The easy accessibility of the polar surface layer relative to the deep subsurface make these viable locations to search for evidence of life.     

The role of mud volcanoes in the evolution of Hecate Tholus Volcano on the surface of Mars

Hecate Tholus Volcano has undergone numerous changes in its history of evolution. Further, the phenomena occurring on the surface of this volcano endow us with a remarkable perspective of recent martian geological changes. In the vicinity of this volcano cone, most of the lava is covered with a thick layer of loose sediments (probably clay). The presence of such sediments at the base of the volcano cone has led to the formation of several major landslides. Moreover, liquid water flow on the volcano cone has created a myriad of radial channels. The formation of such structures on the cone of a volcano is only plausible as a result of eruption of a mud volcano from its crater. Besides, the constant discharge of mud-like materials as well as hot water from the volcano paves the way for the growth and evolution of hydrothermal organisms.     

FY-2C星在天气与环境监测服务中的应用

介绍了风云二号(FY-2)C气象卫星地面应用系统应用服务中心(ASC)的功能.分析了ASC利用C星监测资料生成如热带气旋监测、雾监测、土壤湿度、火情监测、水情监测、积雪监测和海冰监测等部分人机交互产品的原理和处理方法.实际应用结果表明,C星资料在环境与灾害性天气的监测分析、预报和预警等方面发挥了重要作用.     

Spectral signatures of photosynthesis. I. Review of Earth organisms

Why do plants reflect in the green and have a "red edge" in the red, and should extrasolar photosynthesis be the same? We provide (1) a brief review of how photosynthesis works, (2) an overview of the diversity of photosynthetic organisms, their light harvesting systems, and environmental ranges, (3) a synthesis of photosynthetic surface spectral signatures, and (4) evolutionary rationales for photosynthetic surface reflectance spectra with regard to utilization of photon energy and the planetary light environment. We found the "near-infrared (NIR) end" of the red edge to trend from blue-shifted to reddest for (in order) snow algae, temperate algae, lichens, mosses, aquatic plants, and finally terrestrial vascular plants. The red edge is weak or sloping in lichens. Purple bacteria exhibit possibly a sloping edge in the NIR. More studies are needed on pigment-protein complexes, membrane composition, and measurements of bacteria before firm conclusions can be drawn about the role of the NIR reflectance. Pigment absorbance features are strongly correlated with features of atmospheric spectral transmittance: P680 in Photosystem II with the peak surface incident photon flux density at approximately 685 nm, just before an oxygen band at 687.5 nm; the NIR end of the red edge with water absorbance bands and the oxygen A-band at 761 nm; and bacteriochlorophyll reaction center wavelengths with local maxima in atmospheric and water transmittance spectra. Given the surface incident photon flux density spectrum and resonance transfer in light harvesting, we propose some rules with regard to where photosynthetic pigments will peak in absorbance: (1) the wavelength of peak incident photon flux; (2) the longest available wavelength for core antenna or reaction center pigments; and (3) the shortest wavelengths within an atmospheric window for accessory pigments. That plants absorb less green light may not be an inefficient legacy of evolutionary history, but may actually satisfy the above criteria.     

Tírez lake as a terrestrial analog of Europa

Tírez Lake (La Mancha, central Spain) is proposed as a terrestrial analogue of Europa's ocean. The proposal is based on the comparison of the hydrogeochemistry of Tírez Lake with the geochemical features of the alteration mineralogy of meteoritic precursors and with Galileo's Near Infrared Mapping Spectrometer data on Europa's surface. To validate the astrobiological potential of Tírez Lake as an analog of Europa, different hydrogeochemical, mineral, and microbial analyses were performed. Experimental and theoretical modeling helped to understand the crystallization pathways that may occur in Europa's crust. Calculations about the oxidation state of the hypothetical Europan ocean were estimated to support the sulfate-rich neutral liquid model as the origin of Europa's observed hydrated minerals and to facilitate their comparison with Tírez's hydrogeochemistry. Hydrogeochemical and mineralogical analyses showed that Tírez waters corresponded to Mg-Na-SO(4)-Cl brines with epsomite, hexahydrite, and halite as end members. A preliminary microbial ecology characterization identified two different microbial domains: a photosynthetically sustained community represented by planktonic/benthonic forms and microbial mat communities, and a subsurficial anaerobic realm in which chemolithotrophy predominates. Fluorescence in situ hybridization has been used to characterize the prokaryotic diversity of the system. The subsurficial community seemed to be dominated by sulfate-reducing bacteria and methanogens. Frozen Tírez brines were analyzed by Fourier-transform infrared techniques providing spectra similar to those reported previously using pure components and to the Galileo spectral data. Calorimetric measurements of Tírez brines showed pathways and phase metastability for magnesium sulfate and sodium chloride crystallization that may aid in understanding the processes involved in the formation of Europa's icy crust. The use of fluorescence hybridization techniques for microbial detection and characterization in hypersaline environments makes this methodology strongly advisable for future Europa astrobiological missions.     

Carbonaceous cherts in the Barberton greenstone belt and their significance for the study of early life in the Archean record

The 3.5-3.2 Ga old volcano-sedimentary succession of the Barberton greenstone belt (South Africa) is characterized by lithological units that are repeated in a regular manner. Komatiitic, basaltic, and dacitic volcanic and volcaniclastic sequences are capped by zones of silica enrichment, followed by bedded carbonaceous cherts. Stratiform and crosscutting carbonaceous chert veins are common in silica alteration zones and bedded cherts. A detailed field study of several chert horizons and chert veins that range in age from 3.47 to 3.30 Ga revealed the importance of syndepositional hydrothermal activity for their origin. Bedded cherts consist of silicified detrital and tuffaceous sediments that were deposited on the seafloor. Silicification took place at the sediment-water interface as a result of diffuse upflow of low-temperature hydrothermal fluids, which gave rise to the formation of impermeable chert caps. Fluid overpressure resulted in the breaching of the cap rocks at times. Chert veins contain angular host rock fragments, replace wall rocks, and show evidence of multiple vein fillings and in situ brecciation of earlier generations of vein fillings. They represent hydraulic fractures that were initiated by overpressuring of the hydrothermal system. The vein networks were infilled, partly by hydrothermal chert precipitates, and partly by still unconsolidated (not yet silicified) sedimentary material derived from overlying sedimentary horizons. Field, petrographic, isotopic, and trace element evidence indicate that most carbonaceous matter represents sedimentary material that originated by biogenic processes in the Archean oceans and not by hydrothermal processes in the subsurface.     

不同密度弹丸对水冰的超高速撞击成坑实验

为了研究冰冻天体表面撞击坑的形成与演化，开展了水冰的超高速撞击成坑实验。使用二级轻气炮发射1.0 mm直径的球形弹丸，以3 km/s、5 km/s和7 km/s速度对圆柱状冰块进行撞击。弹丸材料包括聚碳酸酯和不锈钢两种，冰块温度为253 K。实验观察到了不同弹丸和不同速度条件下，冰块中撞击坑的形貌特征。对撞击坑直径、深度和剖面形状进行了测量，并与文献中铝弹丸对水冰的撞击坑进行了比较分析。获得了水冰撞击坑特征随撞击参数的变化规律，结果表明：撞击坑直径和深度的主导机制不同，坑深主要由弹丸侵彻作用形成，而坑径主要由冰块的剥落所致；坑深比坑径具有更强的对于弹丸密度的依赖性，高密度弹丸撞击坑直径具有比低密度弹丸更强的对于撞击速度的依赖性；撞击坑体积与撞击能量成正比，高密度弹丸形成的撞击坑直径表现出“能量缩比”行为，而低密度弹丸形成的撞击坑直径表现出“动量缩比”行为。     

FY-1C在地表灾情和环境监测中的应用

介绍了国家气象中心一年来利用FY-1C极轨气象卫星开展地表监测和环境监测包括火情监测、水情监测、积雪监测、海冰监测及植被监测的情况。指出由于该卫星探测通道比美国NOAA卫星多、过境时机适宜，因而在气象卫星应用于地表灾情和环境监测方面具有明显的优势。     

On laboratory simulation and the effect of small temperature oscillations about the freezing point and ice formation on the evaporation rate of water on Mars

We report measurements of the evaporation rate of water under Mars-like conditions (CO2 atmosphere at 7 mbar and approximately 0 degrees C) in which small temperature oscillations about the freezing point repeatedly formed and removed a thin layer of ice. We found that the average evaporation at 2.7 +/- 0.5 degrees C without an ice layer (corrected for the difference in gravity on Earth and on Mars) was 1.24 +/- 0.12 mm/h, while at -2.1 +/- 0.3 degrees C with an ice layer the average evaporation rate was 0.84 +/- 0.08 mm/h. These values are in good agreement with those calculated for the evaporation of liquid water and ice when it is assumed that evaporation only depends on diffusion and buoyancy. Our findings suggest that such differences in evaporation rates are entirely due to the temperature difference and that the ice layer has little effect on evaporation rate. We infer that the formation of thin layers of ice on pools of water on Mars does not significantly increase the stability of water on the surface of Mars.     

The potential for lithoautotrophic life on Mars: application to shallow interfacial water environments

We developed a numerical model to assess the lithoautotrophic habitability of Mars based on metabolic energy, nutrients, water availability, and temperature. Available metabolic energy and nutrient sources were based on a laboratory-produced Mars-analog inorganic chemistry. For this specific reference chemistry, the most efficient lithoautotrophic microorganisms would use Fe(2+) as a primary metabolic electron donor and NO(3)(-) or gaseous O(2) as a terminal electron acceptor. In a closed model system, biomass production was limited by the electron donor Fe(2+) and metabolically required P, and typically amounted to approximately 800 pg of dry biomass/ml ( approximately 8,500 cells/ml). Continued growth requires propagation of microbes to new fecund environments, delivery of fresh pore fluid, or continued reaction with the host material. Within the shallow cryosphere--where oxygen can be accessed by microbes and microbes can be accessed by exploration-lithoautotrophs can function within as little as three monolayers of interfacial water formed either by adsorption from the atmosphere or in regions of ice stability where temperatures are within some tens of degrees of the ice melting point. For the selected reference host material (shergottite analog) and associated inorganic fluid chemistry, complete local reaction of the host material potentially yields a time-integrated biomass of approximately 0.1 mg of dry biomass/g of host material ( approximately 10(9) cells/g). Biomass could also be sustained where solutes can be delivered by advection (cryosuction) or diffusion in interfacial water; however, both of these processes are relatively inefficient. Lithoautotrophs in near-surface thin films of water, therefore, would optimize their metabolism by deriving energy and nutrients locally. Although the selected chemistry and associated model output indicate that lithoautotrophic microbial biomass could accrue within shallow interfacial water on Mars, it is likely that these organisms would spend long periods in maintenance or survival modes, with instantaneous biomass comparable to or less than that observed in extreme environments on Earth.     

HNIW中间体纯度测定方法

建立了测定六硝六氮杂异戊兹烷基(HNIW)两个主要中间体四乙酰基六氮杂异戊兹烷(TAIW)和四乙酰基二苄基六氮杂异戊兹烷(TADB IW)纯度的高效液相色谱法。采用二极管阵列紫外检测器检测,检测波长230 nm;中间体TAIW采用水作溶剂,DE-613反相色谱柱,甲醇流动相分离;中间体TADB IW采用冰醋酸溶解后水稀释,Symm etry-C18反相色谱柱,甲醇-水流动相分离。结果表明,两中间体分析方法标准偏差均小于1.0%,标准样品回收率均在100.0%左右,准确度和精密度较好,满足HNIW生产工艺和质量控制要求。     

Olivine-respiring bacteria isolated from the rock-ice interface in a lava-tube cave, a Mars analog environment

The boundary between ice and basalt on Earth is an analogue for some near-surface environments of Mars. We investigated neutrophilic iron-oxidizing microorganisms from the basalt-ice interface in a lava tube from the Oregon Cascades with perennial ice. One of the isolates (Pseudomonas sp. HerB) can use ferrous iron Fe(II) from the igneous mineral olivine as an electron donor and O(2) as an electron acceptor. The optimum growth temperature is ～12-14°C, but growth also occurs at 5°C. Bicarbonate is a facultative source of carbon. Growth of Pseudomonas sp. HerB as a chemolithotrophic iron oxidizer with olivine as the source of energy is favored in low O(2) conditions (e.g., 1.6% O(2)). Most likely, microbial oxidation of olivine near pH 7 requires low O(2) to offset the abiotic oxidation of iron. The metabolic capabilities of this bacterium would allow it to live in near-surface, icy, volcanic environments of Mars in the present or recent geological past and make this type of physiology a prime candidate in the search for life on Mars.     

Habitable zone limits for dry planets

Most discussion of habitable planets has focused on Earth-like planets with globally abundant liquid water. For an "aqua planet" like Earth, the surface freezes if far from its sun, and the water vapor greenhouse effect runs away if too close. Here we show that "land planets" (desert worlds with limited surface water) have wider habitable zones than aqua planets. For planets at the inner edge of the habitable zone, a land planet has two advantages over an aqua planet: (i) the tropics can emit longwave radiation at rates above the traditional runaway limit because the air is unsaturated and (ii) the dry air creates a dry stratosphere that limits hydrogen escape. At the outer limits of the habitable zone, the land planet better resists global freezing because there is less water for clouds, snow, and ice. Here we describe a series of numerical experiments using a simple three-dimensional global climate model for Earth-sized planets. Other things (CO(2), rotation rate, surface pressure) unchanged, we found that liquid water remains stable at the poles of a low-obliquity land planet until net insolation exceeds 415 W/m(2) (170% that of modern Earth), compared to 330 W/m(2) (135%) for the aqua planet. At the outer limits, we found that a low-obliquity land planet freezes at 77%, while the aqua planet freezes at 90%. High-obliquity land and aqua planets freeze at 58% and 72%, respectively, with the poles offering the last refuge. We show that it is possible that, as the Sun brightens, an aqua planet like Earth can lose most of its hydrogen and become a land planet without first passing through a sterilizing runaway greenhouse. It is possible that Venus was a habitable land planet as recently as 1 billion years ago.     

SOLID3: a multiplex antibody microarray-based optical sensor instrument for in situ life detection in planetary exploration

The search for unequivocal signs of life on other planetary bodies is one of the major challenges for astrobiology. The failure to detect organic molecules on the surface of Mars by measuring volatile compounds after sample heating, together with the new knowledge of martian soil chemistry, has prompted the astrobiological community to develop new methods and technologies. Based on protein microarray technology, we have designed and built a series of instruments called SOLID (for "Signs Of LIfe Detector") for automatic in situ detection and identification of substances or analytes from liquid and solid samples (soil, sediments, or powder). Here, we present the SOLID3 instrument, which is able to perform both sandwich and competitive immunoassays and consists of two separate functional units: a Sample Preparation Unit (SPU) for 10 different extractions by ultrasonication and a Sample Analysis Unit (SAU) for fluorescent immunoassays. The SAU consists of five different flow cells, with an antibody microarray in each one (2000 spots). It is also equipped with an exclusive optical package and a charge-coupled device (CCD) for fluorescent detection. We demonstrated the performance of SOLID3 in the detection of a broad range of molecular-sized compounds, which range from peptides and proteins to whole cells and spores, with sensitivities at 1-2?ppb (ng?mL?1) for biomolecules and 10? to 103 spores per milliliter. We report its application in the detection of acidophilic microorganisms in the Río Tinto Mars analogue and report the absence of substantial negative effects on the immunoassay in the presence of 50?mM perchlorate (20 times higher than that found at the Phoenix landing site). Our SOLID instrument concept is an excellent option with which to detect biomolecules because it avoids the high-temperature treatments that may destroy organic matter in the presence of martian oxidants.