Effects of a simulated martian UV flux on the cyanobacterium, Chroococcidiopsis sp. 029

Dried monolayers of Chroococcidiopsis sp. 029, a desiccation-tolerant, endolithic cyanobacterium, were exposed to a simulated martian-surface UV and visible light flux, which may also approximate to the worst-case scenario for the Archean Earth. After 5 min, there was a 99% loss of cell viability, and there were no survivors after 30 min. However, this survival was approximately 10 times higher than that previously reported for Bacillus subtilis. We show that under 1 mm of rock, Chroococcidiopsis sp. could survive (and potentially grow) under the high martian UV flux if water and nutrient requirements for growth were met. In isolated cells, phycobilisomes and esterases remained intact hours after viability was lost. Esterase activity was reduced by 99% after a 1-h exposure, while 99% loss of autofluorescence required a 4-h exposure. However, cell morphology was not changed, and DNA was still detectable by 4',6-diamidino-2-phenylindole staining after an 8-h exposure (equivalent to approximately 1 day on Mars at the equator). Under 1 mm of simulant martian soil or gneiss, the effect of UV radiation could not be detected on esterase activity or autofluorescence after 4 h. These results show that under the intense martian UV flux the morphological signatures of life can persist even after viability, enzymatic activity, and pigmentation have been destroyed. Finally, the global dispersal of viable, isolated cells of even this desiccation-tolerant, ionizing-radiation-resistant microorganism on Mars is unlikely as they are killed quickly by unattenuated UV radiation when in a desiccated state. These findings have implications for the survival of diverse microbial contaminants dispersed during the course of human exploratory class missions on the surface of Mars.     

Survival of amino acids in micrometeorites during atmospheric entry

The delivery of amino acids by micrometeorites to the early Earth during the period of heavy bombardment could have been a significant source of the Earth's prebiotic amino acid inventory provided that these organic compounds survived atmospheric entry heating. To investigate the sublimation of amino acids from a micrometeorite analog at elevated temperature, grains from the CM-type carbonaceous chondrite Murchison were heated to 550 degrees C inside a glass sublimation apparatus (SA) under reduced pressure. The sublimed residue that had collected on the cold finger of the SA after heating was analyzed for amino acids by HPLC. We found that when the temperature of the meteorite reached approximately 150 degrees C, a large fraction of the amino acid glycine had vaporized from the meteorite, recondensed onto the end of the SA cold finger, and survived as the rest of the grains heated to 550 degrees C. alpha-Aminoisobutryic acid and isovaline, which are two of the most abundant non-protein amino acids in Murchison, did not sublime from the meteorite and were completely destroyed during the heating experiment. Our experimental results suggest that sublimation of glycine present in micrometeorite grains may provide a way for this amino acid to survive atmospheric entry heating at temperatures > 550 degrees C; all other amino acids apparently are destroyed.     

Spectral signatures of photosynthesis. II. Coevolution with other stars and the atmosphere on extrasolar worlds

As photosynthesis on Earth produces the primary signatures of life that can be detected astronomically at the global scale, a strong focus of the search for extrasolar life will be photosynthesis, particularly photosynthesis that has evolved with a different parent star. We take previously simulated planetary atmospheric compositions for Earth-like planets around observed F2V and K2V, modeled M1V and M5V stars, and around the active M4.5V star AD Leo; our scenarios use Earth's atmospheric composition as well as very low O2 content in case anoxygenic photosynthesis dominates. With a line-by-line radiative transfer model, we calculate the incident spectral photon flux densities at the surface of the planet and under water. We identify bands of available photosynthetically relevant radiation and find that photosynthetic pigments on planets around F2V stars may peak in absorbance in the blue, K2V in the red-orange, and M stars in the near-infrared, in bands at 0.93-1.1 microm, 1.1-1.4 microm, 1.5-1.8 microm, and 1.8-2.5 microm. However, underwater organisms will be restricted to wavelengths shorter than 1.4 microm and more likely below 1.1 microm. M star planets without oxygenic photosynthesis will have photon fluxes above 1.6 microm curtailed by methane. Longer-wavelength, multi-photo-system series would reduce the quantum yield but could allow for oxygenic photosystems at longer wavelengths. A wavelength of 1.1 microm is a possible upper cutoff for electronic transitions versus only vibrational energy; however, this cutoff is not strict, since such energetics depend on molecular configuration. M star planets could be a half to a tenth as productive as Earth in the visible, but exceed Earth if useful photons extend to 1.1 microm for anoxygenic photosynthesis. Under water, organisms would still be able to survive ultraviolet flares from young M stars and acquire adequate light for growth.     

Damage escape and repair in dried Chroococcidiopsis spp. from hot and cold deserts exposed to simulated space and martian conditions

The cyanobacterium Chroococcidiopsis, overlain by 3?mm of Antarctic sandstone, was exposed as dried multilayers to simulated space and martian conditions. Ground-based experiments were conducted in the context of Lichens and Fungi Experiments (EXPOSE-E mission, European Space Agency), which were performed to evaluate, after 1.5 years on the International Space Station, the survival of cyanobacteria (Chroococcidiopsis), lichens, and fungi colonized on Antarctic rock. The survival potential and the role played by protection and repair mechanisms in the response of dried Chroococcidiopsis cells to ground-based experiments were both investigated. Different methods were employed, including evaluation of the colony-forming ability, single-cell analysis of subcellular integrities based on membrane integrity molecular and redox probes, evaluation of the photosynthetic pigment autofluorescence, and assessment of the genomic DNA integrity with a PCR-based assay. Desiccation survivors of strain CCMEE 123 (coastal desert, Chile) were better suited than CCMEE 134 (Beacon Valley, Antarctica) to withstand cellular damage imposed by simulated space and martian conditions. Exposed dried cells of strain CCMEE 123 formed colonies, maintained subcellular integrities, and, depending on the exposure conditions, also escaped DNA damage or repaired the induced damage upon rewetting.     

Survival of rock-colonizing organisms after 1.5 years in outer space

Cryptoendolithic microbial communities and epilithic lichens have been considered as appropriate candidates for the scenario of lithopanspermia, which proposes a natural interplanetary exchange of organisms by means of rocks that have been impact ejected from their planet of origin. So far, the hardiness of these terrestrial organisms in the severe and hostile conditions of space has not been tested over extended periods of time. A first long-term (1.5 years) exposure experiment in space was performed with a variety of rock-colonizing eukaryotic organisms at the International Space Station on board the European EXPOSE-E facility. Organisms were selected that are especially adapted to cope with the environmental extremes of their natural habitats. It was found that some-but not all-of those most robust microbial communities from extremely hostile regions on Earth are also partially resistant to the even more hostile environment of outer space, including high vacuum, temperature fluctuation, the full spectrum of extraterrestrial solar electromagnetic radiation, and cosmic ionizing radiation. Although the reported experimental period of 1.5 years in space is not comparable with the time spans of thousands or millions of years believed to be required for lithopanspermia, our data provide first evidence of the differential hardiness of cryptoendolithic communities in space.     

Microbial rock inhabitants survive hypervelocity impacts on Mars-like host planets: first phase of lithopanspermia experimentally tested

The scenario of lithopanspermia describes the viable transport of microorganisms via meteorites. To test the first step of lithopanspermia, i.e., the impact ejection from a planet, systematic shock recovery experiments within a pressure range observed in martian meteorites (5-50 GPa) were performed with dry layers of microorganisms (spores of Bacillus subtilis, cells of the endolithic cyanobacterium Chroococcidiopsis, and thalli and ascocarps of the lichen Xanthoria elegans) sandwiched between gabbro discs (martian analogue rock). Actual shock pressures were determined by refractive index measurements and Raman spectroscopy, and shock temperature profiles were calculated. Pressure-effect curves were constructed for survival of B. subtilis spores and Chroococcidiopsis cells from the number of colony-forming units, and for vitality of the photobiont and mycobiont of Xanthoria elegans from confocal laser scanning microscopy after live/dead staining (FUN-I). A vital launch window for the transport of rock-colonizing microorganisms from a Mars-like planet was inferred, which encompasses shock pressures in the range of 5 to about 40 GPa for the bacterial endospores and the lichens, and a more limited shock pressure range for the cyanobacterium (from 5-10 GPa). The results support concepts of viable impact ejections from Mars-like planets and the possibility of reseeding early Earth after asteroid cataclysms.     

Influence on photosynthesis of starlight, moonlight, planetlight, and light pollution (reflections on photosynthetically active radiation in the universe)

Photosynthesis on Earth can occur in a diversity of organisms in the photosynthetically active radiation (PAR) range of 10 nmol of photons m(-2) s(-1) to 8 mmol of photons m(-2) s(-1). Similar considerations would probably apply to photosynthetic organisms on Earth-like planets (ELPs) in the continuously habitable zone of other stars. On Earth, starlight PAR is inadequate for photosynthetically supported growth. An increase in starlight even to reach the minimum theoretical levels to allow for photosynthesis would require a universe that was approximately ten million times older, or with a ten million times greater density of stars, than is the case for the present universe. Photosynthesis on an ELP using PAR reflected from a natural satellite with the same size as our Moon, but at the Roche limit, could support a low rate of photosynthesis at full Moon. Photosynthesis on an ELP-like satellite of a Jupiter-sized planet using light reflected from the planet could be almost 1% of the rate in full sunlight on Earth when the planet was full. These potential contributions to photosynthesis require that the contribution is compared with the rate of photosynthesis driven by direct radiation from the star. Light pollution on Earth only energizes photosynthesis by organisms that are very close to the light source. However, effects of light pollution on photosynthesis can be more widespread if the photosynthetic canopy is retained for more of the year, caused by effects on photoperiodism, with implications for the influence of civilizations on photosynthesis.     

Common freshwater cyanobacteria grow in 100% CO2

Cyanobacteria and similar organisms produced most of the oxygen found in Earth's atmosphere, which implies that early photosynthetic organisms would have lived in an atmosphere that was rich in CO2 and poor in O2. We investigated the tolerance of several cyanobacteria to very high (>20 kPa) concentrations of atmospheric CO2. Cultures of Synechococcus PCC7942, Synechocystis PCC7942, Plectonema boryanum, and Anabaena sp. were grown in liquid culture sparged with CO2-enriched air. All four strains grew when transferred from ambient CO2 to 20 kPa partial pressure of CO2 (pCO2), but none of them tolerated direct transfer to 40 kPa pCO2. Synechococcus and Anabaena survived 101 kPa (100%) pCO2 when pressure was gradually increased by 15 kPa per day, and Plectonema actively grew under these conditions. All four strains grew in an anoxic atmosphere of 5 kPa pCO2 in N2. Strains that were sensitive to high CO2 were also sensitive to low initial pH (pH 5-6). However, low pH in itself was not sufficient to prevent growth. Although mechanisms of damage and survival are still under investigation, we have shown that modern cyanobacteria can survive under Earth's primordial conditions and that cyanobacteria-like organisms could have flourished under conditions on early Mars, which probably had an atmosphere similar to early Earth's.     

Bacillus subtilis spore survival and expression of germination-induced bioluminescence after prolonged incubation under simulated Mars atmospheric pressure and composition: implications for planetary protection and lithopanspermia

Bacterial endospores in the genus Bacillus are considered good models for studying interplanetary transfer of microbes by natural or human processes. Although spore survival during transfer itself has been the subject of considerable study, the fate of spores in extraterrestrial environments has received less attention. In this report we subjected spores of a strain of Bacillus subtilis, containing luciferase resulting from expression of an sspB-luxAB gene fusion, to simulated martian atmospheric pressure (7-18 mbar) and composition (100% CO(2)) for up to 19 days in a Mars simulation chamber. We report here that survival was similar between spores exposed to Earth conditions and spores exposed up to 19 days to simulated martian conditions. However, germination-induced bioluminescence was lower in spores exposed to simulated martian atmosphere, which suggests sublethal impairment of some endogenous spore germination processes.     

火星探测器降落伞拉直过程中的“绳帆”现象研究

针对火星探测器降落伞在拉直过程中出现的“绳帆”现象,以及火星探测器降落伞开伞前初始参数和大气密度与地球环境下的差异,建立了火星探测器降落伞拉直过程的数学模型,研究了火星环境下,伞包弹射速度、开伞前进入器的攻角、开伞马赫数以及大气密度对“绳帆”现象的影响。研究结果表明,选择较大的伞包弹射速度,并将开伞前进入器的攻角严格限定在较小范围内,将有利于避免或降低“绳帆”现象的发生。这一研究结果可为我国实施火星探测时减速着陆系统的设计分析提供一定参考。     

Optimal trajectories for the aeroassisted flight experiment

This paper deals with the determination of optimal trajectories for the aeroassisted flight experiment (AFE). The intent of this experiment is to simulate a GEO-to-LEO transfer, where GEO denotes a geosynchronous Earth orbit and LEO denotes a low Earth orbit. Specifically, the AFE spacecraft is released from the Space Shuttle and is accelerated by means of a solid rocket motor toward Earth, so as to achieve atmospheric entry conditions identical with those of a spacecraft returning from GEO. During the atmospheric pass, the angle of attack is kept constant, and the angle of bank is controlled in such a way that the following conditions are satisfied: (a) the atmospheric velocity depletion is such that, after exiting, the AFE spacecraft first ascends to a specified apogee and then descends to a specified perigee; and (b) the exit orbital plane is identical with the entry orbital plane. The final maneuver, not analyzed here, includes the rendezvous with and the capture by the Space Shuttle. In this paper, the trajectories of an AFE spacecraft are analyzed in a 3D space, employing the full system of 6 ODEs describing the atmospheric pass. The atmospheric entry conditions are given, and the atmospheric exit conditions are adjusted in such a way that requirements (a) and (b) are met, while simultaneously minimizing the total characteristic velocity, hence the propellant consumption required for orbital transfer. Two possible transfers are considered: indirect ascent (IA) to a 178 NM perigee via a 197 NM apogee; and direct ascent (DA) to a 178 NM apogee. For both transfers, two cases are investigated: (i) the bank angle is continuously variable; and (ii) the trajectory is divided into segments along which the bank angle is constant. For case (ii), the following subcases are studied; 2, 3, 4 and 5 segments; because the time duration of each segment is optimized, the above subcases involve 4, 6, 8 and 10 parameters, respectively. It is shown that the optimal trajectories of cases (i) and (ii) coalesce into a single trajectory: a two-subarc trajectory, with the bank angle constant in each subarc (bang-bang control). Specifically, the bank angle is near 180° in the atmospheric entry phase (positive lift projection phase) and is near 0° in the atmospheric exit phase (negative lift projection phase). It is also shown that, during the atmospheric pass, the peak values of the changes of the orbital inclination and the longitude of the ascending node are nearly zero; hence, the peak value of the wedge angle (angle between the instantaneous orbital plane and the initial orbital plane) is nearly zero. This means that the motion of the spacecraft is nearly planar in an inertial space.     

行星际空间环境对探测器可靠性影响分析

空间环境是影响航天器可靠性的重要因素。与地球轨道航天器相比,行星际探测任务可能会遭受更加恶劣的空间环境,例如极端温度环境,辐射环境,腐蚀性大气环境、宇宙尘等,再加上行星际任务寿命长,采用先进的器件和材料,空间环境对行星际探测器的可靠性构成严重的威胁,直接关系到探测目标能否实现。因此考虑空间环境对行星际探测器的影响,开展相关的预先研究无论是对于制定行星际空间探测计划,还是搭载仪器的设计都具有非常重要的意义。文章分析了极端温度、辐射环境和行星表面综合环境对探测器的影响,并对开展相关研究提出了建议。     

NEOs as stepping stones to Mars and main-belt asteroids

Human interplanetary missions are constrained by the problem of astronaut exposure to galactic cosmic radiation. This paper surveys the existing on-line near-Earth object (NEO) data base in an effort to identify NEOs that cross both Earth's ad Mars’ orbits and could be used as cosmic ray shields by interplanetary voyagers. The search concentrated on low-inclination Mars-crossing NEOs that approach Earth, Mars, and main-belt asteroids in the 2020–2100 time frame. Both outbound and return transfers were searched for. Several candidates for Earth–Mars, Mars–Earth, and Earth–Vesta transfers have been found from the very incomplete August 2008 data base. Other aspects of this interplanetary transfer option are considered.     

Bacillus subtilis spores on artificial meteorites survive hypervelocity atmospheric entry: implications for Lithopanspermia

An important but untested aspect of the lithopanspermia hypothesis is that microbes situated on or within meteorites could survive hypervelocity entry from space through Earth's atmosphere. The use of high-altitude sounding rockets to test this notion was explored. Granite samples permeated with spores of Bacillus subtilis strain WN511 were attached to the exterior telemetry module of a sounding rocket and launched from White Sands Missile Range, New Mexico into space, reaching maximum atmospheric entry velocity of 1.2 km/s. Maximum recorded temperature during the flight was measured at 145 degrees C. The surfaces of the post-flight granite samples were swabbed and tested for recovery and survival of WN511 spores, using genetic markers and the unique DNA fingerprint of WN511 as recovery criteria. Spore survivors were isolated at high frequency, ranging from 1.2% to 4.4% compared with ground controls, from all surfaces except the forward-facing surface. Sporulation-defective mutants were noted among the spaceflight survivors at high frequency (4%). These experiments constitute the first report of spore survival to hypervelocity atmospheric transit, and indicate that sounding rocket flights can be used to model the high-speed atmospheric entry of bacteria-laden artificial meteorites.     

Penetration of solar cosmic rays into the Earth’s magnetosphere on January 28, 2012

Results of the comparative analysis of the dynamics of SCR fluxes with energies of 1–100 MeV in the interplanetary environment according to the data of the ACE and Wind spacecraft and within the Earth’s magnetosphere according to the data of the GOES-15 and Electro-L satellites in the region of geostationary orbits, and POES-19 and Meteor-M1 in the region of polar caps during two increases in SCR of January 19–31, 2012, are presented. It is shown that the decrease in the efficiency of SCR penetration into the Earth’s magnetosphere in the region of the orbits under study on January 28, 2012, is related to the passage of the Earth’s magnetosphere through the interplanetary environment structure with a quasi-radial interplanetary magnetic field and a small pressure of the solar wind.     

The solution of a model problem of the atmospheric entry of a small meteoroid

Direct simulation Monte Carlo modeling (DSMC) is used to solve the problem of the entry into the Earth’s atmosphere of a small meteoroid. The main aspects of the physical theory of meteors, such as mass loss (ablation) and effects of aerodynamic and thermal shielding, are considered based on the numerical solution of the model problem of the atmospheric entry of an iron meteoroid. The DSMC makes it possible to obtain insight into the structure of the disturbed area around the meteoroid (coma) and trace its evolution depending on entry velocity and height (Knudsen number) in a transitional flow regime where calculation methods used for free molecular and continuum regimes are inapplicable.     

Endolithic cyanobacteria in halite rocks from the hyperarid core of the Atacama Desert

In the driest parts of the Atacama Desert there are no visible life forms on soil or rock surfaces. The soil in this region contains only minute traces of bacteria distributed in patches, and conditions are too dry for cyanobacteria that live under translucent stones. Here we show that halite evaporite rocks from the driest part of the Atacama Desert are colonized by cyanobacteria. This colonization takes place just a few millimeters beneath the rock surface, occupying spaces among salt crystals. Our work reveals that these communities are composed of extremely resistant Chroococcidiopsis morphospecies of cyanobacteria and associated heterotrophic bacteria. This newly discovered endolithic environment is an extremely dry and, at the same time, saline microbial habitat. Photosynthetic microorganisms within dry evaporite rocks could be an important and previously unrecognized target for the search for life within our Solar System.     

Low cost planetary exploration: surrey lunar minisatellite and interplanetary platform missions

In order to meet the growing global requirement for affordable missions beyond Low Earth Orbit, two types of platform are under design at the Surrey Space Centre. The first platform is a derivative of Surrey's UoSAT-12 minisatellite, launched in April 1999 and operating successfully in-orbit. The minisatellite has been modified to accommodate a propulsion system capable of delivering up to 1700 m/s delta-V, enabling it to support a wide range of very low cost missions to LaGrange points, Near-Earth Objects, and the Moon. A mission to the Moon - dubbed “MoonShine” - is proposed as the first demonstration of the modified minisatellite beyond LEO. The second platform - Surrey's Interplanetary Platform - has been designed to support missions with delta-V requirements up to 3200 m/s, making it ideal for low cost missions to Mars and Venus, as well as Near Earth Objects (NEOs) and other interplanetary trajectories. Analysis has proved mission feasibility, identifying key challenges in both missions for developing cost-effective techniques for: spacecraft propulsion; navigation; autonomous operations; and a reliable safe mode strategy. To reduce mission risk, inherently failure resistant lunar and interplanetary trajectories are under study. In order to significantly reduce cost and increase reliability, both platforms can communicate with low-cost ground stations and exploit Surrey's experience in autonomous operations. The lunar minisatellite can provide up to 70 kg payload margin in lunar orbit for a total mission cost US$16–25 M. The interplanetary platform can deliver 20 kg of scientific payload to Mars or Venus orbit for a mission cost US$25–50 M. Together, the platforms will enable regular flight of payloads to the Moon and interplanetary space at unprecedented low cost. This paper outlines key systems engineering issues for the proposed Lunar Minisatellite and interplanetary Platform Missions, and describes the accommodation and performance offered to planetary payloads.     

General solution for the optimal trajectory of an AFE-type spacecraft

The aeroassisted flight experiment (AFE) refers to an experimental spacecraft to be launched and then recovered by the Space Shuttle. It simulates a transfer from a geosynchronous Earth orbit (GEO) to a low Earth orbit (LEO). In this paper, with reference to an AFE-type spacecraft, an actual GEO-to-LEO transfer is considered under the following assumptions: the GEO and LEO orbital planes are identical; both the initial and final orbits are circular; the initial phase angle is given, while the final phase angle is free. The aeroassisted orbital transfer trajectory involves three branches: a preatmospheric branch, GEO-to-entry; an atmospheric branch, entry-to-exit; a post-atmospheric branch, exit-to-LEO. The optimal trajectory is determined by minimizing the total characteristic velocity. The optimization is performed with respect to the velocity impulses at GEO, LEO, and the time history of the angle of bank during the atmospheric pass. It is assumed that the entry path inclination is free and that the angle of attack is constant, = 17.0 deg. The sequential gradient-restoration algorithm is used to compute the optimal trajectory and it is shown that the best atmospheric pass is to be performed with constant angle of bank. The resulting optimal trajectory constitutes an ideal nominal trajectory for the generation of guidance trajectories for two reasons: the fact that the low value of the characteristic velocity is accompanied by relatively low values of the peak heating rate and the peak dynamic pressure; and the simplicity of the control distribution, requiring constant angle of bank.     

Role of the ionosphere for the atmospheric evolution of planets

We have synthesized current understanding, mainly observations, with regard to ion escape mechanisms to space from the ionosphere and exosphere of Titan and Earth-type planets, with the intent to provide an improved input for models of atmospheric evolution on early Earth and Earth-type planets and exoplanets. We focus on the role of the ionosphere and its non-linear response to solar parameters, all of which have been underestimated in current models of ancient atmospheric escape (4 billion years ago). Factors that have been overlooked include the following: (1) Much larger variation of O(+) outflow than H(+) outflow from the terrestrial ionosphere, depending on solar and geomagnetic activities (an important consideration when attempting to determine the oxidized state of the atmosphere of early Earth); (2) magnetization of the ionopause, which keeps ionospheric ions from escaping and controls many other escape processes; (3) extra ionization by, for example, the critical ionization velocity mechanism, which expands the ionosphere to greater altitudes than current models predict; and (4) the large escape of cold ions from the dense, expanded ionosphere of Titan. Here we offer, as a guideline for quantitative simulations, a qualitative diagnosis of increases or decreases of non-thermal escape related to the ionosphere for magnetized and unmagnetized planets in response to changes in solar parameters (i.e., solar EUV/FUV flux, solar wind dynamic pressure, and interplanetary magnetic field).