On the quality of mutations in mammalian cells induced by high LET radiations

The deleterious effects of accelerated heavy ions as component of the space radiation environment on living cells are of increasing importance for long duration human space flight activities. The most important aspect of such densely ionizing particle radiation is attributed to the type and quality of biological damage induced by them. This issue is addressed by investigating cell inactivation and mutation induction at the Hprt locus (coding for hypoxanthine-guanine-phosphoribosyl-transferase) of cultured V79 Chinese hamster cells exposed to densely ionizing radiation (accelerated heavy ions with different LETs from oxygen to gold, specific energies ranging from 1.9 to 69.7 MeV/u, corresponding LET values range from 62 to 13,223 keV/μm) and to sparsely ionizing radiation (200 kV X-rays). 30 spontaneous, 40 X-ray induced and 196 heavy ion induced 6-thioguanine resistant Hprt mutant colonies were characterized by Southern technique using the restriction enzymes EcoRI, PstI and BglII and a full length Hprt cDNA probe isolated from the plasmid pHPT12. Restriction patterns of the spontaneous Hprt mutants were indistinguishable from the wild type pattern, as these mutants probably contain only small deletions or even point mutations in the Hprt locus. In contrast, the overall spectrum of heavy ion induced mutations revealed a majority of partial or total deletions of the Hprt gene. With constant particle fluence (3 × 10⁶ particles/cm²) the quality of heavy ion induced mutations in the Hprt locus depends on physical parameters of the beam (atomic number, specific energy, LET). This finding suggests a relationship between the type of DNA damage and track structure. The fraction of mutants with severe deletions in the Hprt locus after exposure to oxygen ions increases from 65% at 60 keV/μm up to a maximum (100%) at 300 keV/μm and declines with higher LET values to 75% at 750 keV/μm. With heavier ions (Ca- and Au-ions) and even higher LET-values this mutant fraction decreases to 58% at 13,200 keV/μm. Heavy ion induced DNA break points in the Hprt locus are not randomly distributed.     

Comparison of genotoxic damage in monolayer cell cultures and three-dimensional tissue-like cell assemblies

Assessing the biological risks associated with exposure to the high-energy charged particles encountered in space is essential for the success of long-term space exploration. Although prokaryotic and eukaryotic cell models developed in our laboratory and others have advanced our understanding of many aspects of genotoxicity, in vitro models are needed to assess the risk to humans from space radiation insults. Such models must be representative of the cellular interactions present in tissues and capable of quantifying genotoxic damage. Toward this overall goal, the objectives of this study were to examine the effect of the localized microenvironment of cells, cultured as either 2-dimensional (2D) monolayers or 3-dimensional (3D) aggregates, on the rate and type of genotoxic damage resulting from exposure to Fe-charged particles, a significant portion of space radiation. We used rodent transgenic cell lines containing 50–70 copies of a LacI transgene to provide the enhanced sensitivity required to quantify mutational frequency and type in the 1100-bp LacI target as well as assessment of DNA damage to the entire 45-kbp construct. Cultured cells were exposed to high-energy Fe charged particles at Brookhaven National Laboratory’s Alternating Gradient Synchrotron facility for a total dose ranging from 0.1 to 2 Gy and allowed to recover for 0–7 days, after which mutational type and frequency were evaluated. The mutational frequency was found to be higher in 3D samples than in 2D samples at all radiation doses. Mutational frequency also was higher at 7 days after irradiation than immediately after exposure. DNA sequencing of the mutant targets revealed that deletional mutations contributed an increasingly high percentage (up to 27%) of all mutations in cells as the dose was increased from 0.5 to 2 Gy. Several mutants also showed large and complex deletions in multiple locations within the LacI target. However, no differences in mutational type were found between the 2D and the 3D samples. These 3D tissue-like model systems can reduce the uncertainty involved in extrapolating risk between in vitro cellular and in vivo models.     

Analysis of electromagnetic and electrostatic effects of particle impacts on spacecraft

Particle impacts on spacecraft can cause considerable damage, even leading to complete failure. A theory for the resulting vehicle potential changes and the electromagnetic radiation from impact-induced plasma has been published by Close et al. (2010). Here we compare this theory to impacts registered by the Radio and Plasma Wave Science instrumentation on the Cassini spacecraft. We study both low-velocity (16 km/s) large particles (2.6 μm radius) detected in Saturn’s rings and high-velocity (450 km/s) small particles (1 nm radius) in the solar wind. The agreement with the theory is quite good. We also apply these results to earth orbit and conclude that both Electrostatic Discharge and Electromagnetic Pulse radiation are likely and could lead to spacecraft failure.     

Modeling of effect of polarization on UV sky radiance during twilight

The spatial distribution of the vector of the Stokes parameters characterizing the radiance intensity and the radiance polarization describes the radiation field in the atmosphere. A simplified treatment of light as the scalar has only restricted application. A few studies compared previously results of the vector and scalar radiative transfer models and showed that scalar models are in error by up to 10% for many cases. Though several observational conditions were exploited, an effect of polarization on modeling of UV radiance has not been investigated yet for twilight. The paper presents a preliminary study of modeled UV radiance during twilight taking into account polarization. The intensity and the degree of linear polarization of the scattered UV radiance for two cases of the ground-based observations are discussed. In the first case, radiation incoming from the zenith for the solar zenith angles (SZA) from 90° to 98° is under investigation. Radiation in the solar principal plane for the beginning of twilight (SZA = 90.1°) was calculated in the second case. The study showed that the UV radiation field in the twilight atmosphere can be handled correctly only using the vector theory. The errors of scalar radiative transfer strongly depend on wavelength, line of an observation and solar position. The revealed distortion of the zenith radiance caused by using of the scalar approximation reaches maximum of 15% at 340 nm for the solar zenith angle (SZA) equal to 98°. The shorter wavelengths have the smaller errors, about 5% at 305 nm for SZA = 98°, due to the larger part of the single scattered radiance. The error of the scalar modeling may be as large as −17% for radiance incoming from the horizon for SZA = 90.1°. Scalar radiative transfer models underestimate the integral intensity in the principal plane up to 3–4% ± 0.5% at SZA = 90.1° for wavelengths from 320 to 340 nm. This should be taken into account in problems of radiative budget estimation and remote sensing of the atmosphere exploiting the twilight period.     

Genetic susceptibility to radiation.

In the context of space radiation, it is important to know whether the human population includes genetically predisposed radiosensitive subsets. One possibility is that haploinsufficiency for ATM confers radiosensitivity, and this defect involves 1-3% of the population. Using knock-out mice we chose to study cataractogenesis in the lens and oncogenic transformation in mouse embryo fibroblasts to assay for effects of ATM deficiency. Radiation induced cataracts appeared earlier in the heterozygous versus wild-type animals following exposure to either gamma rays or 1 GeV/nucleon iron ions. In addition, it was found that embryo fibroblasts of Atm heterozygotes showed an increased incidence of oncogenic transformation compared with their normal litter-matched counterparts. From these data we suggest that Ataxia Telangiectasia heterozygotes could indeed represent a societally significant radio sensitive subpopulation. Knock-out mice are now available for other genes including BRCA1 and 2, and Mrad9. An exciting possibility is the creation of double heterozygotes for pairs of mutated genes that function in the same signal transduction pathway, and consequently confer even greater radiosensitivity.     

Hypervelocity impacts on HST solar arrays and the debris and meteoroids population

Accurate debris and meteoroid flux models are crucial for the design of manned and unmanned space missions. For the most abundant particle sizes smaller than a few millimetres, knowledge of the populations can only be gained from in situ detectors or the analysis of retrieved space hardware. The measurement of impact flux from exposed surfaces improves with increased surface area and exposure time.A post-flight impact investigation was initiated by the European Space Agency to record and analyse the impact fluxes and any potential resulting damage on the two flexible solar arrays of the Hubble Space Telescope. The arrays were deployed during the first Hubble Space Telescope servicing mission in December 1993 and retrieved in March 2002. They have a total exposed surface area of roughly 120 m², including 42 m² covered with solar cells. This new Hubble post-flight impact study follows a similar activity undertaken after the retrieval of one of the first solar arrays, in 1993. The earlier study provided the first opportunity for a numerical survey of damage to exposed surfaces from more than 600 km altitude, and of impacts from particles larger than 1 mm. The results have proven very valuable in validation of important flux model regimes. The second set of Hubble solar arrays has again provided an unrivalled opportunity to measure the meteoroid and debris environment, now sampled during a long interval in low Earth orbit, and to identify changes in the space debris environment since the previous survey. The retrieved solar array wings exhibit thousands of craters, many of which are visible to the naked eye. A few hundred impacts have completely penetrated the 0.7 mm thick array. The largest impact features are about 7–8 mm in diameter. The cover glass of the solar cells is particularly well suited to the recognition of small impact features by optical and electron microscopy. In this paper, we present the first results of the impact survey. Data upon the abundance of craters of specific measured size ranges are plotted as cumulative flux curves, and compared to the results of model predictions. The most significant change to the particle flux since 1993 is a decrease in the small debris population.     

Dependence of E-sporadic layer response on solar and geomagnetic activity variations from its ion composition

The aim of this paper is to consider an influence of solar and geomagnetic activity level variations on frequency and stochastic parameters of mid-latitude ionosphere sporadic-E layer. Critical frequency of sporadic-E layer, foEs, relative excess of sporadic ionization over monthly median values, foEs − foEm/foEm, and probability of Es layer appearance, PEs, are considered. It has been found that sporadic-E layer parameters response to solar and geomagnetic activity level variations can be both positive (foEs and PEs values are increase) and negative (foEs and PEs values are decrease). In particular, sporadic-E layers response to solar and geomagnetic activity variations are different depending upon layer intensity. It is suggested that revealed differences may be associated with dissimilarity of the layers ion composition (high-intensive layers are composed from metallic ions and low intensive composed from molecular ions).     

Radiosensitivity to high energy iron ions is influenced by heterozygosity for Atm, Rad9 and Brca1

Loss of function of DNA repair genes has been implicated in the development of many types of cancer. In the last several years, heterozygosity leading to haploinsufficiency for proteins involved in DNA repair was shown to play a role in genomic instability and carcinogenesis after DNA damage is induced, for example by ionizing radiation. Since the effect of heterozygosity for one gene is relatively small, we hypothesize that predisposition to cancer could be a result of the additive effect of heterozygosity for two or more genes critical to pathways that control DNA damage signaling, repair or apoptosis. We investigated the role of heterozygosity for Atm, Rad9 and Brca1 on cell oncogenic transformation and cell survival induced by 1 GeV/n⁵⁶Fe ions. Our results show that cells heterozygous for both Atm and Rad9 or Atm and Brca1 have high survival rates and are more sensitive to transformation by high energy iron ions when compared with wild-type controls or cells haploinsufficient for only one of these proteins. Since mutations or polymorphisms for similar genes exist in a small percentage of the human population, we have identified a radiosensitive sub-population. This finding has several implications. First, the existence of a radiosensitive sub-population may distort the shape of the dose–response relationship. Second, it would not be ethical to put exceptionally radiosensitive individuals into a setting where they may potentially be exposed to substantial doses of radiation.     

Cataractogenesis from high-LET radiation and the Casarett model.

Space radiations, especially heavy ions, constitute significant hazards to astronauts. These hazards will increase as space missions lengthen. Moreover, the dangers to astronauts will be enhanced by the persistence, or even the progression, of biological damage throughout their subsequent life spans. To assist in the assessment of risks to astronauts, we are investigating the long-term effects of heavy ions on specific animal tissues. In one study, the eyes of rabbits of various ages were exposed to a single dose of Bragg plateau 20Ne ions (LET infinity approximately equals 30 keV/micrometer). The development of cataracts has shown a pronounced age-related response during the first year after irradiation, and will be followed for two more years. In other studies, mice were exposed to single or fractionated doses of 12C ions (4-cm spread-out Bragg peak; dose-averaged LET infinity = 70-80 keV/micrometer) or 60Co gamma-photons (LET infinity = 0.3 keV/micrometer). Measurements of the frequency of posterior lens opacification have shown that the tissue sparing observed with dose fractionation of gamma-photons was absent when 12C-ion doses were fractionated. Development of posterior lens cataracts was also followed for long periods (up to 21 months) in mice exposed to single doses of Bragg plateau HZE particles (40Ar, 20Ne and 12C ions: LET infinity approximately equals 100, 30 and 10 keV/micrometer, respectively) or 225 kVp X-rays. Based on average cataract levels at the different observation times, the RBE's (RBE = relative biological effectiveness) for the ions were circa 5, 3 and 1-2, respectively, over the range of doses used (0.05-0.9 Gy). Investigations of cataractogenesis are useful for exploring the model of radiation damage proposed by Casarett and by Rubin and Casarett with a tissue not connected directly to the vasculature.     

Launching of a 500,000 cubic meter balloon with the semi-dynamic launching method

Launching a large balloon in a limited launching field is a long standing problem in Japan. The largest balloon ever launched successfully was 200,000 m³ in volume. It was launched in 1973. A larger balloon with a volume of 500,000 m³ was tried later, but it burst during the ascending phase. For launching balloons with a large lift exceeding 500 kg, the conventional static launching method had the most serious problem with possible damage to the polyethylene film of the balloon caused by the holding mechanism. After that, we had developed a new static launching method to launch balloons with a total lift of 1.0 ton. For launching a large balloon with a total lift above 1.5 ton, the new static launching method had a weak point in that if there was an air bubble in the folded part of the balloon, it may puncture the balloon as it is pushed by a spool. To avoid this problem, we developed a semi-dynamic launching method in 1999 using a launcher fixed to the ground leaving a freedom of rotation around the vertical axis. We have launched some balloons using the method and have gradually enriched our experience in using this system.In 2003, we successfully launched a balloon with a volume of 500,000 m³ by using the method. This balloon was made of polyethylene films with a thickness of 20 μm and it is the largest balloon ever launched in Japan.     

From mechanisms to risk estimation--bridging the chasm.

We have a considerable amount of work ahead of us to determine the importance of the wealth of new information emerging in the fields of sub-cellular, cellular and tissue biology in order to improve the estimation of radiation risk at low dose and protracted dose-rate. In this paper, we suggest that there is a need to develop models of the specific health effects of interest (e.g., carcinogenesis in specific tissues), which embody as much of the mechanistic (i.e., biological) information as is deemed necessary. Although it is not realistic to expect that every radiation-induced process should or could be included, we can hope that the major factors that shape the time dependence of evolution of damage can be identified and quantified to the point where reasonable estimations of risk can be made. Regarding carcinogenesis in particular, the structure of the model itself plays a role in determining the relative importance of various processes. We use a specific form of a multi-stage carcinogenic model to illustrate this point. We show in a review of the application of this model to lung cancer incidence and mortality in two exposed populations that for both high- and low-LET radiation, there is evidence of an "inverse dose-rate" or protraction effect. This result could be of some considerable importance, because it would imply that risk from protracted exposure even to low-LET radiation might be greater than from acute exposure, an opinion not currently held in the radiation protection community. This model also allows prediction of the evolution of the risk over the lifetimes of the exposed individuals. One inference is that radiation-induced initiation (i.e., the first cellular carcinogenic event(s) occurring in normal tissue after the passage of the radiation) may not be the driving factor in the risk, but more important may be the effects of the radiation on already-initiated cells in the tissue. Although present throughout the length of the exposure, radiation-induced initiation appears to play a dominating role only very late in life, and only for those individuals who began their exposure early in life. These conclusions are very dependent, of course, on the hypotheses embodied in the initiation-promotion-conversion paradigm of carcinogenesis. We suggest that recently identified processes, such as the "bystander effect", might affect initiation, promotion, and malignant conversion in different ways. Finally, the manner in which the quality of radiation affects these processes must be understood in the context of the mixed high- and low-LET radiations that are found in the space environment. Important directions in critical experiment definition are suggested, including a renewed emphasis on well-designed animal experiments over extended periods of time.     

Simultaneous exposure of mammalian cells to heavy ions and X-rays.

Crews of space missions are exposed to a mixed radiation field, including sparsely and densely ionizing radiation. To determine the biological effectiveness of mixed high-/low-LET radiation fields, mammalian cells were exposed in vitro simultaneously to X-rays and heavy ions, accelerated at the HIMAC accelerator. X-ray doses ranged from 1 to 11 Gy. At the same time, cells were exposed to either 40Ar (550 MeV/n, 86 keV/micrometers), 28Si (100 MeV/n, 150 keV/micrometers), or 56Fe (115 MeV/n, 442 keV/micrometers) ions. Survival was measured in hamster V79 fibroblasts. Structural aberrations in chromosome 2 were measured by chemical-induced premature chromosome condensation combined with fluorescence in situ hybridization in isolated human lymphocytes. For argon and silicon experiments, measured damage in the mixed radiation field was consistent with the value expected using an additive function for low- and high-LET separated data. A small deviation from a simple additive function is observed with very high-LET iron ions combined to X-rays.     

The influence of dose, dose-rate and particle fragmentation on cataract induction by energetic iron ions.

Because activities in space necessarily involve chronic exposure to a heterogeneous charged particle radiation field it is important to assess the influence of dose-rate and the possible modulating role of heavy particle fragmentation on biological systems. Using the well-studied cataract model, mice were exposed to plateau 600 MeV/amu 56Fe ions either as acute or fractionated exposures at total doses of 5 - 504 cGy. Additional groups of mice received 20, 360 and 504 cGy behind 50 mm of polyethylene, which simulates body shielding. The reference radiation consisted of 60Co gamma radiation. The animals were examined by slit lamp biomicroscopy over their three year life spans. In accordance with our previous observations with heavy particles, the cataractogenic potential of the 600 MeV/amu 56Fe ions was greater than for low-LET radiation and increased with decreasing dose relative to gamma-rays. Fractionation of a given dose of 56Fe ions did not reduce the cataractogenicity of the radiation compared to the acute regimen. Fragmentation of the beam in the polyethylene did not alter the cataractotoxicity of the ions, either when administered singly or in fractions.     

A radio study of the mouse,G359.23 − 0.82

The recent detection of a young pulsar powering “the Mouse”, G359.23 − 0.82, as well as detailed imaging of surrounding nebular X-ray emission, have motivated us to investigate the structural details and polarization characteristics of the radio emission from this axisymmetric source with a supersonic bow shock. Using polarization data at 3.6 and 6 cm, we find that the magnetic field wraps around the bow-shock structure near the apex of the system, but downnstream runs parallel to the inferred direction of the pulsar’s motion. The rotation measure (RM) distribution of the Mouse also suggests that the low degree of polarization combined with a high RM ahead of the pulsar result from internal plasma within the bow-shock region. In addition, using sub-arcsecond radio image of the Mouse, we identify modulations in the brightness distribution of the Mouse that may be associated with the unshocked pulsar wind behind the pulsar. Lastly, we discuss the relationship between the Mouse and its neighboring shell-type supernova remnant G359.1 − 0.5 and argue that these two sources could potentially have the same origin.     

Study of >100 GeV electrons with BETS detector using a long duration balloon flight in Antarctica

We have observed cosmic-ray electrons from 10 to 1000 GeV by a long duration balloon flight using Polar Patrol Balloon (PPB) in Antarctica. The observation was carried out for 13 days at an altitude of 35 km in January 2004. The detector is an imaging calorimeter composed of scintillating-fiber belts and plastic scintillators inserted between lead plates. The geometrical factor of detector is about 600 cm²sr and the total thickness of lead absorber is 9 radiation lengths. The performance of the detector has been confirmed by the CERN-SPS beam test and also investigated by Monte-Carlo simulations. New telemetry system using a commercial satellite of iridium, power supply by solar batteries, and automatic level control using CPU have successfully been developed and operated during the flight. We have collected 5.7 × 10³ events over 100 GeV including nearly 100 candidates of primary electrons.     

The nature of nonthermal X-ray filaments near the galactic center

Recent Chandra and XMM-Newton observations reported evidence of two X-ray filaments G359.88−0.08 (SgrA-E) and G359.54+0.18 (the ripple filament) near the Galactic center. The X-ray emission from these filaments has a nonthermal spectrum and coincides with synchrotron emitting radio sources. Here, we report the detection of a new X-ray feature coincident with a radio filament G359.90−0.06 (SgrA-F) and show more detailed VLA, Chandra and BIMA observations of the radio and X-ray filaments. In particular, we show that radio emission from the nonthermal filaments G359.90−0.06 (SgrA-F) and G359.54+0.18 (the ripple) has a steep spectrum whereas G359.88−0.08 (SgrA-E) has a flat spectrum. The X-ray emission from both these sources could be due to synchrotron radiation. However, given that the 20 km s⁻¹ molecular cloud, with its intense 1.2 mm dust emission, lies in the vicinity of SgrA-F, it is possible that the X-rays could be produced by inverse Compton scattering of far-infrared photons from dust by the relativistic electrons responsible for the radio synchrotron emission. The production of X-ray emission from ICS allows an estimate of the magnetic field strength of 0.08 mG within the nonthermal filament. This should be an important parameter for any models of the Galactic center nonthermal filaments.     

The total ozone and UV solar radiation over Stara Zagora,Bulgaria

The results from direct ground-based solar UV irradiance measurements and the total ozone content (TOC) over Stara Zagora (42° 25′N, 25° 37′E), Bulgaria are presented. During the period 1999–2003 the TOC data show seasonal variations, typical for the middle latitudes – maximum in the spring and minimum in the autumn. The comparison between TOC ground-based data and Global Ozone Monitoring Experiment (GOME) satellite-borne ones shows a seasonal dependence of the differences between them.A strong negative relationship between the total ozone and the 305 nm wavelength irradiance was found. The dependence between the two variables is significant (r = −0.62 ± 0.18) at 98% confidence level.The direct sun UV doses for some specific biological effects (erythema and eyes) are obtained. The estimation of the radiation amplification factor RAF shows that the ozone reduction by 1% increases the erythemal dose by 2.3%. The eye-damaging doses are more influenced by the TOC changes and in this case RAF = −2.7%.The amount of these biological doses depended on the solar altitude over the horizon. This dependence was not so strong when the total ozone content in the atmosphere was lower.     

Multi-year behaviour of the midnight OH1 temperature according to observations at Zvenigorod over 2000–2016

Using spectral measurements of the hydroxyl airglow at the Zvenigorod station (56° N, 37° E), Moscow region, over 2000–2016, we obtained the long-term set of data comprising 1822 midnight values of the OH¹ temperature in the mesopause region. These data revealed a 17-year series of its mean annual values, as well as amplitudes and phases of the first two harmonics of its annual variation. The obtained parameters were analyzed to determine statistically relevant characteristics of their long-term variations. As a result, we found that the long-term behaviour of the mean annual OH¹ temperature features a small negative linear trend (?0.07 ± 0.03 K/year) over the addressed period. Besides, its dependence on solar activity is shown to be 4.1 ± 0.5 K/100 SFU. Regarding the long-term behaviour of the mean annual OH¹ temperature, we revealed the existence of two oscillations with 3-year (the amplitude being 1.3 ± 0.2 K) and 4.1-year (the amplitude being 0.6 ± 0.2 K) periods. We obtained empirical relations describing year-to-year variations in the amplitudes and phases of the annual and semi-annual harmonics.     

Measurement of turbulent kinetic energy dissipation rates in the mesosphere by a 3 MHz Doppler radar

A new narrow beam Doppler radar operating at 3.17 MHz has been installed close to the Andøya Rocket Range in Andenes, Norway in summer 2002 in order to improve the ground based capabilities for measurements of turbulence in the mesosphere. The main feature of the radar is a Mills Cross transmitting/receiving antenna consisting of 29 crossed half-wave dipoles. In combination with the modular transceiver system this provides high flexibility in beam forming and pointing. In general, vertical and oblique beams with a minimum one way half-power full-beam width (HPFW) of 6.6° are used. The observations are usually performed with a height resolution of 1 km and with off-zenith beams at 7.3° directed towards NW, NE, SE, and SW. Turbulence intensities have been estimated from the width of the observed signal spectra using an computationally intensive correction method which requires precise knowledge of the antenna radiation pattern. The program uses real-time measurements of the wind field in all determinations. Turbulent kinetic energy dissipation rates based on radar observations are presented and compared with corresponding climatological summer and winter profiles from rocket measurements, as well as with single profiles from model runs for selected periods from September 2003 to Summer 2004. The mean turbulent kinetic energy dissipation rates based on these radar measurements are about 5 mW/kg at 60 km altitude and about 20 mW/kg at 80 km, in reasonable agreement with mean turbulence intensities obtained from previous rocket soundings at Andenes.     

Relationship of solar flare accelerated particles to solar energetic particles (SEPs) observed in the interplanetary medium

Observations of hard X-ray (HXR)/γ-ray continuum and γ-ray lines produced by energetic electrons and ions, respectively, colliding with the solar atmosphere, have shown that large solar flares can accelerate ions up to many GeV and electrons up to hundreds of MeV. Solar energetic particles (SEPs) are observed by spacecraft near 1 AU and by ground-based instrumentation to extend up to similar energies as in large SEP events, but it appears that a different acceleration process, one associated with fast coronal mass ejections is responsible. Much weaker SEP events are observed that are generally rich in electrons, ³He, and heavy elements. The energetic particles in these events appear to be similar to those accelerated in flares. The Ramaty high energy solar spectroscopic imager (RHESSI) mission provides high-resolution spectroscopy and imaging of flare HXRs and γ-rays. Such observations can provide information on the location, energy spectra, and composition of the flare accelerated energetic particles at the Sun. Here, preliminary comparisons of the RHESSI observations with observations of both energetic electron and ion near 1 AU are reviewed, and the implications for the particle acceleration and escape processes are discussed.