Peculiarities of moisture transfer in capillary-porous soil substitutes during space flight.

Aboard the orbital stations, "Salyut-7" and "Mir", investigations on the peculiarities of moisture transfer in capillary-porous bodies (CPB; that is, soil substitutes) in microgravity were conducted by the authors with a specially designed device and an original method. Water distribution in CPB-perlite (fractions 1.5... 2.5 mm) was studied, and theoretical dependances for linear capillary impregnation of CPB were determined.     

Effect of the space environment on the induction of DNA-repair related proteins and recovery from radiation damage.

Recovery of bacterial cells from radiation damage and the effects of microgravity were examined in an STS-79 Shuttle/Mir Mission-4 experiment using the extremely radioresistant bacterium Deinococcus radiodurans. The cells were irradiated with gamma rays before the space flight and incubated on board the Space-Shuttle. The survival of the wild type cells incubated in space increased compared with the ground controls, suggesting that the recovery of this bacterium from radiation damage was enhanced under microgravity. No difference was observed for the survival of radiosensitive mutant rec30 cells whether incubated in space or on the ground. The amount of DNA-repair related RecA protein induced under microgravity was similar to those of ground controls, however, induction of PprA protein, the product of a newly found gene related to the DNA repair mechanism of D. radiodurans, was enhanced under microgravity compared with ground controls.     

In vitro plant cell growth in microgravity and on clinostat.

For the study of gravity's role in the processes of plant cell differentiation in-vitro, a model "seed-seedling-callus" has been used. Experiments were carried out on board the orbital stations Salyut-7 and Mir as well as on clinostat. They lasted from 18 to 72 days. It was determined that the exclusion of a one-sided action of gravity vector by means of clinostat and spaceflight conditions does not impede the formation and growth of callus tissue; however, at cell and subcellular levels structural and functional changes do take place. No significant changes were observed either on clinostat or in space concerning the accumulation of fresh biomass, while the percentage of dry material in space is lower than in control. Both in microgravity (MG) and in control, even after 72 days of growth, cells with a normally developed ultrastructure are present. In space, however, callus tissue more often contains cells in which the cross-section area of a cell, a nuclei and of mitochondria are smaller and the vacuole area--bigger than in controls. In microgravity a considerable decrease in the number of starch-containing cells and a reduction in the mean area of starch grains in amyloplasts is observed. In space the amount of soluble proteins in callus tissue is 1.5 times greater than in control. However, no differences were observed in fractions when separated by the SDS-PAGE method. In microgravity the changes in cell wall material components was noted. In the space-formed callus changes in the concentration of ions K, Na, Mg, Ca and P were observed. However, the direction of these changes depends on the age of callus. Discussed are the possible reasons for modification of morphological and metabolic parameters of callus cells when grown under changed gravity conditions.     

大鼠后肢去负荷体位调节装置设计与实验研究

通过对大鼠尾吊模型进行改进，研制出一种新型可调节体位的大鼠后肢去负荷悬吊装置，研究模拟微重力效应下体液分布变化对大鼠骨代谢的影响.将36只SD大鼠均分为对照组（CON）、头低位后肢去负荷组（HDT）、水平位后肢去负荷组（HH）和头高位后肢去负荷组（HUT）4组，实验21天后，利用DXA检测大鼠的骨密度（BMD）.模拟微重力效应下的三组大鼠后肢均发生严重骨丢失，其中HH和HUT组后肢BMD显著大于HDT组.实验结果表明，体液分布变化可能在模拟微重力效应导致的骨丢失中起到重要作用，新型大鼠后肢去负荷悬吊装置能够调节大鼠体位（体液）进行模拟微重力效应研究.      

Canopy photosynthesis and transpiration in microgravity: gas exchange measurements aboard Mir.

The SVET Greenhouse on-board the Orbital Station Mir was used to measure canopy photosynthesis and transpiration rates for the first time in space. During the Greenhouse IIB experiment on Mir (June-January 1997), carbon and water vapor fluxes from two wheat (cv. Superdwarf) canopies were measured using the US developed Gas Exchange Measurement System (GEMS). Gas analyzers capable of resolving CO2 concentration differences of 5 micromoles mol-1 against a background of 0.9% CO2, are necessary to measure photosynthetic and respiratory rates on Mir. The ability of the GEMS gas analyzers to measure these CO2 concentration differences was determined during extensive ground calibrations. Similarly, the sensitivity of the analyzers to water vapor was sufficient to accurately measure canopy evapotranspiration. Evapotranspiration, which accounted for over 90% of the water added to the root zone, was estimated using gas exchange and used to estimate substrate moisture content. This paper presents canopy photosynthesis and transpiration data during the peak vegetative phase of development in microgravity.     

Marangoni and buoyant convection in an open cavity under reduced and under normal gravity

We give the first full analysis of the microgravity experiment MKB ( arangoni onvektion im offenen oot) conducted on board of the space shuttle during the D1-mission in 1985, together with that of some ground based experiments on thermocapillary flow in a rectangular liquid volume with free upper surface. Streamlines and velocity profiles under μ-g and l-g are presented and discussed. Strong thermocapillary flow was observed under μ-g after the rupture of a dirt-film suppressing surface tension forces. The Nusselt-number under microgravity due to thermocapillary flow for Ma = 4.6 · 10⁵ is at least Nu = 6 ± 1. Under l-g a separation of the surface tension driven convection roll of hot fluid circulating on top of colder fluid was found for large Ma. The coupling and decoupling of buoyant and thermocapillary forces has been further studied in an experiment varying the temperature gradient along the free surface independently from that in the bulk fluid.     

空间在轨流体输运双槽道微重力实验装置

空间在轨流体输运双槽道微重力实验装置通过在微重力环境下对开口槽道中的流动进行观察,可以分析研究微重力下流体输运的稳定特性.双槽道形式的实验装置在单次实验中可同时对两种不同截面,不同流量的槽道流动进行观测,同时可有效提升落塔实验效率,减少不同槽道对比实验中的不确定因素.针对双槽道流体实验装置设计的关键问题,例如密封、压力补偿、设备布局等,提出了实验装置的系统结构及落塔实验步骤.在落塔短时微重力环境中,采用氟化液（HFE7500）流体介质,利用本实验装置成功观测到槽道流体输运流动与失稳现象.      

Water movement on the convex surfaces of porous media under microgravity

A plant growth system for crop production under microgravity is part of a life supporting system designed for long-duration space missions. A plant growth in soil in space requires the understanding of water movement in soil void spaces under microgravity. Under 1G-force condition, on earth, water movement in porous media is driven by gradients of matric and gravitational potentials. Under microgravity condition, water movement in porous media is supposed to be driven only by a matric potential gradient, but it is still not well understood. We hypothesized that under microgravity water in void spaces of porous media hardly moved comparing in void spaces without obstacles because the concave surfaces of the porous media hindered water movement. The objective of this study was to investigate water movement on the convex surfaces of porous media under microgravity. We conducted parabolic flight experiments that provided 20–25?s of microgravity at the top of a parabolic flight. We observed water movement in void spaces in soil-like porous media made by glass beads and glass spheres (round-bottomed glass flasks) in the different conditions of water injection under microgravity. Without water injection, water did not move much in neither glass beads nor glass spheres. When water was injected during microgravity, water accumulated in contacts between the particles, and the water made thick fluid films on the particles surface. When the water injection was stopped under microgravity, water was held in the contacts between the particles. This study showed that water did not move upward in the void spaces with or without the water injection. In addition, our results suggested that the difficulty of water movement on the convex (i.e. particle surfaces) might result in slower water move in porous media under microgravity than at 1G-force.     

Development of plant protoplasts during the IML-1 mission.

During the 8 day IML-1 mission, regeneration of cell walls and cell divisions in rapeseed protoplasts were studied using the Biorack microscope onboard the Space Shuttle "Discovery". Samples from microgravity and 1g protoplast cultures were loaded on microscope slides. Visual microscopic observations were reported by the payload specialist Roberta Bondar, by down-link video transmission and by use of a microscope camera. Protoplasts grown under microgravity conditions do regenerate cell walls but to a lesser extent than under 1g. Cell divisions are delayed under microgravity. Few cell aggregates with maximum 4-6 cells per aggregate are formed under microgravity conditions, indicating that microgravity may have a profound influence on plant cell differentiation.     

空间站微重力流体实验设备需求分析

对国际空间站和中国科学实验卫星及载人飞行器上开展的微重力流体实验情况进行论述和分析,重点分析了国际空间站（ISS）微重力流体科学实验设备情况.根据中国空间微重力流体物理科学发展需求,结合国际空间站微重力流体科学实验对设备的需求,提出了未来在中国空间站开展微重力流体实验时空间实验设备需要重点考虑和解决的问题,同时提出相关设计建议.      

"From seed-to-seed" experiment with wheat plants under space-flight conditions.

An important goal with plant experiments in microgravity is to achieve a complete life cycle, the "seed-to-seed experiment." Some Soviet attempts to reach this goal are described, notably an experiment with the tiny mustard, Arabidopsis thaliana, in the Phyton 3 device on Salyut 7. Normal seeds were produced although yields were reduced and development was delayed. Several other experiments have shown abnormalities in plants grown in space. In recent work, plants of wheat (Triticum aestivum) were studied on the ground and then in a preliminary experiment in space. Biometric indices of vegetative space plants were 2 to 2.5 times lower than those of controls, levels of chlorophyll a and b were reduced (no change in the ratio of the two pigments), carotenoids were reduced, there was a serious imbalance in major minerals, and membrane lipids were reduced (no obvious change in lipid patterns). Following the preliminary studies, an attempt was made with the Svetoblock-M growth unit to grow a super-dwarf wheat cultivar through a life cycle. The experiment lasted 167 d on Mir. Growth halted from about day 40 to day 100, when new shoots appeared. Three heads had appeared in the boot (surrounded by leaves) when plants were returned to earth. One head was sterile, but 28 seeds matured on earth, and most of these have since produced normal plants and seeds. In principle, a seed-to-seed experiment with wheat should be successful in microgravity.     

Preparations for CELSS flight experiments with wheat.

We are planning a short-term experiment with Superdwarf wheat on the U.S. Space Shuttle and a seed-to-seed experiment on the Russian Space Station Mir. The goals of both experiments are to observe effects of microgravity on developmental steps in the life cycle and to measure photosynthesis, respiration, and transpiration by monitoring gas exchange. This requires somewhat different hardware development for the two experiments. Ground-based research aims to understand plant responses to the environments in the space growth chambers that we will use (after some modification): the Plant Growth Unit (PGU) on the shuttle and units called Svet, Svetoblock 2, or Oasis on Mir. Low irradiance levels (100 to 250 micromoles m-2 s-1 at best) pose a particular problem. Water and nutrient supply are also potentially limiting factors, especially in the long-term experiment. Our ground-based studies emphasize responses to low light levels (50 to 400 micromoles m-2 s-1); results show that all developmental steps are delayed by low light compared with plants at 400 micromoles m-2 s-1. We are also testing various rooting substrates for the shuttle experiment. A 1:1:1 mixture of peat:perlite:vermiculite appears to be the best choice.     

Effects of exercise equipment on the microgravity environment.

Numerous types of exercise equipment have flown on manned space flights to evaluate and maintain crew members' physical condition while on orbit. Vibrations associated with the use of some exercise equipment cause concern among microgravity scientists who are usually looking for a quiescent environment in which to run their experiments. We discuss the impact of aerobic (bicycle ergometer, treadmill) and non-aerobic (resistance devices) exercise on the microgravity environment of the Space Shuttle Orbiters and the Mir Space Station. In general, characteristic vibration disturbances due to ergometer exercise show the pedalling frequency at 2.5 to 3 Hz and the crew members' body rocking side-to-side at about half the pedalling frequency. For treadmill exercise, the footfall frequency on the treadmill platform can be clearly seen in the 1 to 2 Hz range, along with upper harmonics. The use of resistance exercise devices does not typically cause vibrations. Several vibration isolation systems used on the Orbiters and planned for the International Space Station are introduced. Finally, the responses of specific experiments to exercise vibrations are outlined.     

侧向微重力环境下板式表面张力贮箱内推进剂晃动行为分析

侧向微重力是航天器在轨飞行时在东西位置保持和南北位置保持状态时所处的加速度环境.由于航天器贮箱内推进剂在侧向加速度环境下的重定位过程易产生晃动,因此侧向加速度环境对贮箱内推进剂管理装置(PMD)的管理能力的要求更高.为确保板式贮箱对推进剂的在轨管理能力,需要开展一系列的数值仿真与试验对PMD的管理能力进行验证.本文以板式表面张力贮箱为研究对象,采用VOF两相流模型对侧向微重力环境下重定位过程中贮箱内推进剂的运动进行数值模拟,并对推进剂运动中的晃动行为进行分析.最终,通过数值模拟结果验证了板式表面贮箱的管理能力,为微重力落塔试验与空间站试验提供参考依据.     

Two-dimensional convection in liquid layer related to crystal growth techniques in space

In this paper, the Marangoni convection including buoyancy convection in two-dimensional fluid model was investigated experimentally and theoretically. The transparent liquid in the test cell was heated and cooled under various conditions. The fluid flow driven by surface tension and by buoyancy force was visualized and measured. The flow patterns, velocity distributions and temperature field were obtained and they were compared with the results of the numerical solutions. The effect of the configuration and the convection in microgravity on crystal growth was discussed. The temperature field of the crystallizing surface was predicted.     

微重力板式贮箱推进剂重定位的数值仿真

板式表面张力贮箱内推进剂重定位对确定推进剂分布情况、研究晃动影响、提高控制精度等具有重要意义．为研究板式贮箱内推进剂重定位的规律,对微重力下板式贮箱内液体重定位问题进行数值仿真．计算时使用三维非定常两相流动流体（VOF）模型,对某一板式贮箱寿命末期在不同微重力加速度情况下各种重定位过程进行数值仿真,得到各种工况下重定位的全过程,以及定位后推进剂的分布情况．数值仿真结果为板式贮箱的设计提供有利依据．     

Don Pettit 国际空间站微重力流体实验与若干现象的解析

Don Pettit 是美国NASA 的资深宇航员, 2002 年11 月至2003 年5 月, 在国际空间站工作期间, 进行了一系列名为Saturday Morning Science的微重力实验. Don Pettit 实验揭示了微重力下一些令人惊奇的现象, 涉及流体力学、气泡动力学、晶体生长以及运动学的诸多基础问题, 是能够获得的国际空间站为数不多的微重力实验资料, 具有重要研究意义. 实验包括14 组流体实验和7 组力学实验, 而流体实验又大致分为水膜实验和水球实验, 包括大尺寸水膜制备、扩散和对流、Marangoni 对流、薄膜结晶、水球/气泡旋转等. 本文对Don Pettit 实验的流体部分进行了介绍, 并对其中的Marangoni对流和薄膜结晶实验现象进行了解析.      

Progress on Microgravity Sciences in China

The main progress of the research activities on microgravity fluid physics, combustion, biotechnology research and fundamental Physics in China are briefly summarized in the present paper. The major space missions and experimental results obtained on board the Chinese recoverable/nonrecoverable satellites and the Chinese manned spaceship named "Shen Zhou" are presented summarily. The recent main activities of the ground-based studies in China are introduced in brief.     

空间飞行与回转器回旋条件下青菜开花与花粉发育的研究

比较研究了SJ-8返回式卫星留轨舱微重力条件与地面三维回转模拟微重力条件下青菜生长与发育情况.研究发现空间微重力条件下青菜开花过程需要大约18 h,明显长于地面对照5 h左右.回转器模拟实验结果表明,改变重力影响了花瓣的伸展与发育及花粉的产量,回转条件下花粉细胞中的微管排列明显不同于静止对照.细胞骨架受到干扰可能是改变重力条件下花粉产量降低的原因之一.本研究首次报道了在空间飞行试验中成功地采用了显微实时图像技术观察植物的开花过程,并获得了从花蕾到开花结束各阶段清晰的图像.      

Effects of microgravity on c-fos gene expression in osteoblast-like MC3T3-E1 cells.

The paper summarizes the data on proliferation and gravity-related gene expression of osteoblasts that were obtained from an experiment conducted under simulated and real microgravity conditions. Simulated microgravity conditions obtained in a clinostat depress proliferation of both osteoblast-like MC3T3-E1 and HeLa carcinoma cells. This depression of proliferation occurs in a collagen gel culture in which the flow of culture medium by rotation may be reduced. Interestingly, MC3T3-E1 cells which are probably one of target cells to microgravity are more sensitive than the HeLa cells. Simulated microgravity inhibited the epidermal growth factor (EGF)-induced c-fos gene expression in the MC3T3-El cells. To examine in detail the effect of real microgravity on the EGF signal transduction cascade in osteoblasts, MC3T3-E1 cells were cultured in the Cell Culture Experiment Module of the sounding rocket TR-1A6. The EGF-induced c-fos expression in cells was depressed under short-term microgravity conditions in the sounding rocket, while the phosphorylation of mitogen-activated protein kinase (MAPK) was not affected compared with the controls grown on the ground. These results suggest that an action site of microgravity in the signal transduction pathway may be downstream of MAPK.