Medical considerations for extending human presence in space

The prospects for extending the length of time that humans can safely remain in space depend partly on resolution of a number of medical issues. Physiologic effects of weightlessness that may affect health during flight include loss of body fluid, functional alterations in the cardiovascular system, loss of red blood cells and bone mineral, compromised immune system function, and neurosensory disturbances. Some of the physiologic adaptations to weightlessness contribute to difficulties with readaptation to Earth's gravity. These include cardiovascular deconditioning and loss of body fluids and electrolytes; red blood cell mass; muscle mass, strength, and endurance; and bone mineral. Potentially harmful factors in space flight that are not related to weightlessness include radiation, altered circadian rhythms and rest/work cycles, and the closed, isolated environment of the spacecraft. There is no evidence that space flight has long-term effects on humans, except that bone mass lost during flight may not be replaced, and radiation damage is cumulative. However, the number of people who have spent several months or longer in space is still small. Only carefully-planned experiments in space preceded by thorough ground-based studies can provide the information needed to increase the amount of time humans can safely spend in space.     

Space analogue studies in Antarctica

Medical research has been carried out on the Australian National Antarctic Research Expeditions (ANARE) for 50 years. As an extension of this program collaborative Australian/United States research on immunology, microbiology, psychology and remote medicine has produced important data and insight on how humans adapt to the stress of extreme isolation, confinement and the harsh environment of Antarctica. An outstanding analogue for the isolation and confinement of space missions (especially planetary outposts), ANARE has been used as an international research platform by Australia and the United States since 1993. Collaborative research has demonstrated a lowered responsiveness of the immune system under the isolation and confinement of Antarctic winter-over; a reduction of almost 50% in T cell proliferation to mltogen phytohaemogglutinin, as well as changes in latent herpesvirus states and the expansion of the polyclonal latent Epstein-Barr virus infected B cell populations. Although no clinically significant disease has been found to result from these immune changes, research is currently assessing the effects of psychological factors on the immune system. This and associated research performed to date and its relevance to both organisations is discussed, and comment made on possible extensions to the program in both medical and other fields.     

Whole lichen thalli survive exposure to space conditions: results of Lithopanspermia experiment with Aspicilia fruticulosa

The Lithopanspermia space experiment was launched in 2007 with the European Biopan facility for a 10-day spaceflight on board a Russian Foton retrievable satellite. Lithopanspermia included for the first time the vagrant lichen species Aspicilia fruticulosa from Guadalajara steppic highlands (Central Spain), as well as other lichen species. During spaceflight, the samples were exposed to selected space conditions, that is, the space vacuum, cosmic radiation, and different spectral ranges of solar radiation (λ?≥?110, ≥200, ≥290, or ≥400?nm, respectively). After retrieval, the algal and fungal metabolic integrity of the samples were evaluated in terms of chlorophyll a fluorescence, ultrastructure, and CO(2) exchange rates. Whereas the space vacuum and cosmic radiation did not impair the metabolic activity of the lichens, solar electromagnetic radiation, especially in the wavelength range between 100 and 200?nm, caused reduced chlorophyll a yield fluorescence; however, there was a complete recovery after 72?h of reactivation. All samples showed positive rates of net photosynthesis and dark respiration in the gas exchange experiment. Although the ultrastructure of all flight samples showed some probable stress-induced changes (such as the presence of electron-dense bodies in cytoplasmic vacuoles and between the chloroplast thylakoids in photobiont cells as well as in cytoplasmic vacuoles of the mycobiont cells), we concluded that A. fruticulosa was capable of repairing all space-induced damage. Due to size limitations within the Lithopanspermia hardware, the possibility for replication on the sun-exposed samples was limited, and these first results on the resistance of the lichen symbiosis A. fruticulosa to space conditions and, in particular, on the spectral effectiveness of solar extraterrestrial radiation must be considered preliminary. Further testing in space and under space-simulated conditions will be required. Results of this study indicate that the quest to discern the limits of lichen symbiosis resistance to extreme environmental conditions remains open.     

Effects of space flights on human allergic status (IgE-mediated sensitivity)

Suppression of the immune system after space flights of different duration has been reported earlier by Konstantinova [Immune system in extreme conditions, Space immunology. B. 59. M. Science 1988. 289p. (in Russian) [4]; Immunoresistance of man in space flight, Acta Astronautica 23 (1991) 123–127 [5]]. Changes in T- and B-mediated activities of the immune system were demonstrated during and after space flight. However, the influence of the space flight conditions on the allergic status of cosmonauts and astronauts is still unclear. The goal of this investigation was to analyze total blood IgE levels, specific IgE-antibodies and interleukin-4 in blood of Russian crew members before and after space flights to the International Space Station (ISS) and during a long-term isolation study. For this purpose, we used the ELISA assays as well as other special kits. It was noticed that four out of nine cosmonauts had high total serum IgE (more than normal clinical values of 120 IU/ml). At the same time, there were no statistically significant changes in serum IgE levels before and after long-term space flights (128–195 days). A similar situation was observed regarding preflight IgE levels of cosmonauts who performed short-term flights (7–11 days). However, seven out of 11 cosmonauts had increased IgE level in blood post short flights as compared with pre-flight values. We also measured specific IgE-antibodies, because their high concentration may cause the increased production of total IgE indicating sensitization of cosmonauts. This becomes more important when humans spend a longer time in the closed environment of a space vehicle. Also our ground-based investigations showed that a stay in such conditions does not enhance sensitization to allergens (total number of tested allergens 27) including food, inhalants and cross-reactive proteins. Serum interleukin-4 level measured after short- and long-term space flights was identical. A linear correlation between levels of immunoglobulin E and interleukin-4 also was not significant. Despite the fact that our investigations did not establish any influence of space flight on sensitization and development of immediate-type allergic reactions, they demonstrated the necessity to control the allergic status of cosmonauts very carefully both before and after space flights. At the same time, it is necessary to pay special attention to outcomes of atopic individuals with high pre-flight level of total blood IgE.     

Bone and body mass changes during space flight

Long duration space flight has shown us that humans have significant bone loss and mineral changes because they are living in microgravity. Skylab and the longer Salyut and Mir missions, are providing us useful data and allowing us to explore the mechanism involved in skeletal turnover. Bone redistribution occurs throughout space flight with bone loss predominately in the weight bearing bones of posture and locomotion. The primary health hazards which may occur during space flight induced by skeletal changes include signs and symptoms of hypercalcemia, and the risk of kidney stones and metastatic calcification. After flight lengthy recovery of bone mass and the possible increase in the risk of bone fracture should be considered. Continued research studies are being directed toward determining the mechanisms by which bone is lost in space and developing more effective countermeasures by both the US (Schneider and McDonald, 1984 and Schneider, LeBlanc & Huntoon, 1993) and Russian (Grigoriev et. al., 1989) space programs.     

Radiation: risk and protection in manned space flight.

Space radiation is the primary source of hazard for orbital and interplanetary space flight. Radiation levels for different space mission durations, have been established in order to determine the level of hazard. The risk of exceeding the established levels should not be more than 1%. Radiation environment models have been developed to estimate these values. It is possible to build spacecraft shielding based on the calculation of doses and the risk of exceeding these. By reviewing various calculated estimates of the risk, the radiation hazard and the efficiency of protective measures can be established for specific flights.     

Using radiation risk for assessment of space radiation hazard

Radiation hazard caused by exposure during a spaceflight is characterized by radiobiological consequences at all levels of organism. These consequences have a stochastic nature. Even deterministic effects are basically random quantity having all attributes of such mathematical values. The radiation risk is defined in this case as an additional probability of health damage or as a death probability in extreme case. For the manned spaceflight additional peculiarity of a human’s exposure is added. A natural space radiation environment has a stochastic character because solar particle events and crew of a spacecraft can be exposed to dose from background level up to lethal one.The report presents a procedure of radiation risk assessment for quantitative expression of radiation hazard level during a flight and using this value for developing protection recommendations. It is emphasized that the risk value is connected specifically with the time interval of possible hazard’s existent. The form of risk representation must be chosen depending on a time scale of radiobiological processes induced by the exposure (expressing in fact the radiation hazard model). Surviving function specified for the crewmember mortality rate changed by the professional exposure must be used for risk calculation. Solar particle events determine a stochastic character of radiation environment in space that must be taken into account for a risk assessment. The reliability of radiation risk assessment can be used for this goal.     

A Method to Measure Absorptivity and Emissivity of Temperature-Controlling Coatings of Spacecraft Using Radiation Flux of Variable Intensity

A new method of determining the absorptivity and emissivity of surfaces with the use of a radiation flux of variable intensity is described. It is expedient to use this method in order to control the variations of integral optical coefficients of temperature-controlling coatings in the course of a long-term spacecraft flight. The method is developed on the basis of solving the inverse problem for a mathematical model of thermal balance for a thin metal plate. The stability of the algorithm of processing the results of measurements is analyzed for various levels of random errors. Some recommendations concerning the technical implementation of the method in the laboratory and space conditions are given. The method suggested has a number of advantages in comparison with methods used before, especially if it is used in space conditions. The main advantage is the possibility of varying the radiation flux incident on the surface of a specimen under study from an external source according to any smooth law.     

利用地月间空间站的载人登月飞行模式分析

为提高空间站利用率，降低载人登月任务成本，有效开发地月空间，研究了基于地月空间不同轨道空间站的载人登月飞行模式。首先对比直接往返登月飞行模式，对基于空间站的载人登月飞行模式进行任务分析，通过空间站将载人登月任务解耦为载人天地往返任务和登月任务两部分；其次通过轨道设计和稳定性分析提出考虑登月任务需求的地月间空间站可运行轨道和停泊点；最后建立一套飞行模式评价模型，从速度增量需求、飞行时间、空间环境、登月任务窗口、测控条件、交会对接技术难度、后续任务支持性和任务可靠性方面对6种不同位置空间站的登月飞行模式进行分析和定量评价。评价结果表明基于L2点Halo轨道空间站的载人登月飞行模式为更优飞行模式。     

Radiation protection of astronauts in LEO

Radiological protection for space flights is often perceived as a technico-scientific problem. All this is the result of the effects of radiation encountered in space and manned flight conditions. The main characteristics of this radiation come from its complex composition and its large energy spectrum which must be taken into account as well as flux variations by both solar activity and the vehicle position on orbit. Inside a vehicle, structures constitute irregularly distributed shields and lead to a specific dose at each location. To be able to protect the crew, it is first necessary to understand the threat and therefore to identify the radiation environment: extraterrestrial and orbital. As the environment varies with both the orbit position and time, the dose received in each critical organ during missions must be determined and compared with acceptable limits. To counter the threat, which may exceed acceptable limits, a strategy is required, including the complementary aspects of prevention, detection, protection and possibly treatment.     

Mutagenesis in bacterial spores exposed to space and simulated martian conditions: data from the EXPOSE-E spaceflight experiment PROTECT

As part of the PROTECT experiment of the EXPOSE-E mission on board the International Space Station (ISS), the mutagenic efficiency of space was studied in spores of Bacillus subtilis 168. After 1.5 years' exposure to selected parameters of outer space or simulated martian conditions, the rates of induced mutations to rifampicin resistance (Rif(R)) and sporulation deficiency (Spo(-)) were quantified. In all flight samples, both mutations, Rif(R) and Spo(-), were induced and their rates increased by several orders of magnitude. Extraterrestrial solar UV radiation (>110?nm) as well as simulated martian UV radiation (>200?nm) led to the most pronounced increase (up to nearly 4 orders of magnitude); however, mutations were also induced in flight samples shielded from insolation, which were exposed to the same conditions except solar irradiation. Nucleotide sequencing located the Rif(R) mutations in the rpoB gene encoding the β-subunit of RNA polymerase. Mutations isolated from flight and parallel mission ground reference (MGR) samples were exclusively localized to Cluster I. The 21 Rif(R) mutations isolated from the flight experiment showed all a C to T transition and were all localized to one hotspot: H482Y. In mutants isolated from the MGR, the spectrum was wider with predicted amino acid changes at residues Q469K/L/R, H482D/P/R/Y, and S487L. The data show the unique mutagenic power of space and martian surface conditions as a consequence of DNA injuries induced by solar UV radiation and space vacuum or the low pressure of Mars.     

空间辐射环境工程的现状及发展趋势

空间辐射环境是航天器在轨运行所面临的重要环境要素之一,因其诱发的单粒子效应、总剂量效应、位移损伤效应、表面充放电效应、内带电效应等既可引起航天器材料、器件、结构等在轨损伤、性能退化甚至失效,然而又可以利用其开展空间育种等活动。文章从空间辐射环境与模型、空间辐射效应及机理、空间辐射环境与效应试验的评价标准、空间辐射环境效应试验方法、空间辐射环境与效应地面模拟试验设备、空间辐射环境与效应数值模拟、空间辐射环境与效应飞行试验及抗辐射加固技术等角度对空间辐射环境工程的现状进行了评述,进而提出了空间辐射环境工程各个领域的发展趋势。     

深空辐射环境对航天活动的危害及对策

深空辐射环境是影响深空探测任务的一个主要因素,它主要包括太阳粒子事件、银河宇宙射线、俘获辐射带和黑体表面辐射。文章分析了它对航天活动的危害,并探讨了相应的对策。     

Radiation protection in space

Radiation environment, basic concepts of radiation protection, and specific aspects of the space radiation field are reviewed. The discussion of physico-chemical and subcellular radiation effects includes mechanisms of radiation action and cellular consequences. The discussion of radiobiological effects includes unique aspects of HZE particle effects, space flight findings, terrestrial findings, analysis of somatic radiation effects and effects on critical organs, and early and delayed effects. Other topics include the impact of the space flight environment, measurement of radiation exposure, establishing radiation protection limits, limitations in establishing space-based radiation exposure limits, radiation protection measures, and recommendations.     

On the correlation between individual biochemical parameters of human blood serum following space flight and their basal values

The article presents data concerning the osmolality and concentration of electrolytes and hormones regulating their balance for blood serum of 223 cosmonauts and astronauts. The obtained results allow us to judge the constancy of physicochemical parameters for the blood serum of healthy individuals and how they react to extreme conditions of space flight. The parameters used for evaluation included not just absolute values for the examined indices, but also how they responded to space flight, the dependence on baseline values and the interrelationship between ions. These data are important to predict the effect of exposure to extreme conditions and point to what extent the effect depends on the characteristics of the individual.     

Lichens survive in space: results from the 2005 LICHENS experiment

This experiment was aimed at establishing, for the first time, the survival capability of lichens exposed to space conditions. In particular, the damaging effect of various wavelengths of extraterrestrial solar UV radiation was studied. The lichens used were the bipolar species Rhizocarpon geographicum and Xanthoria elegans, which were collected above 2000 m in the mountains of central Spain and as endolithic communities inhabiting granites in the Antarctic Dry Valleys. Lichens were exposed to space in the BIOPAN-5 facility of the European Space Agency; BIOPAN-5 is located on the outer shell of the Earth-orbiting FOTON-M2 Russian satellite. The lichen samples were launched from Baikonur by a Soyuz rocket on May 31, 2005, and were returned to Earth after 16 days in space, at which time they were tested for survival. Chlorophyll fluorescence was used for the measurement of photosynthetic parameters. Scanning electron microscopy in back-scattered mode, low temperature scanning electron microscopy, and transmission electron microscopy were used to study the organization and composition of both symbionts. Confocal laser scanning microscopy, in combination with the use of specific fluorescent probes, allowed for the assessment of the physiological state of the cells. All exposed lichens, regardless of the optical filters used, showed nearly the same photosynthetic activity after the flight as measured before the flight. Likewise, the multimicroscopy approach revealed no detectable ultrastructural changes in most of the algal and fungal cells of the lichen thalli, though a greater proportion of cells in the flight samples had compromised membranes, as revealed by the LIVE/DEAD BacLight Bacterial Viability Kit. These findings indicate that most lichenized fungal and algal cells can survive in space after full exposure to massive UV and cosmic radiation, conditions proven to be lethal to bacteria and other microorganisms. The lichen upper cortex seems to provide adequate protection against solar radiation. Moreover, after extreme dehydration induced by high vacuum, the lichens proved to be able to recover, in full, their metabolic activity within 24 hours.     

柔性绳网动力学建模与天地差异性分析

采用有限元模型研究了柔性绳网系统的动力学特性。针对空间绳网直接弹射展开方式,首先将绳网离散为若干单元,各单元处理为非线性“半阻尼弹簧”模型,然后分别计算各单元所受气动力和重力,最终建立绳网系统多柔体动力学模型。基于所建立的动力学模型分别对柔性绳网在地面和太空展开的动力学过程进行了仿真分析,研究了绳网在展开面积、空间位形和飞行距离等方面的天地差异性及其动力学机理,为未来空间绳网系统的分析设计提供理论参考。     

Main medical results of extended flights on space station Mir in 1986-1990

During 1986-1990 seven prime spacecrews (16 cosmonauts) have flown on-board the Mir orbital complex. The longest space mission duration was 366 days The principal objectives of the medical tasks were the maintenance of good health and performance of the spacecrews and conducting medical research programs which included study of the cardiovascular, motor, endocrine, blood, immune, and metabolic systems. Results obtained point to the ability of humans to readily adapt to a year-long stay in space and maintain good health and performance. Readaptation had a similar course as after other previous long-term space flights of up to 8 months in duration. Primary body system changes were not qualitatively different from findings after flights aboard the Salyut 6 and 7 space stations. In this case, during and after an 11-12 month flight, body system alterations were even less severe which was a result of adequate countermeasure use, their systematic and creative employment and maintenance of required environments to support life and work in space.     

Recent results of the joint ESA-DARA/IBMP experiments Biokosmos "Seeds"

Comparison of experimental data obtained from short (SDEF) and long duration exposure flights (LDEF) have recently led to results which will be significant for longer and/or repeated sojourn of man in space. Under orbital conditions biological stress and damage are induced in test subjects by cosmic radiation, especially the high energetic, densely ionizing component of heavy ions. Plant seeds were successful model systems for a biotest in studying the physiological damages and mutagenic effect caused by ionizing cosmic radiation in particular stem cells. Dosimetrically, the subdivision into charge- and Let-groups reveals the contribution of the intermediate group (LET = 350-1000 MeV/cm) due to the medium heavy ions (Z = 6-10). Their relative contribution increases with the lower inclination of the orbit of LDEF-1; on the other hand, the total fluence becomes higher with longer duration of the flight. The observed endpoints of the biological radiation damage hint at a correlation with particle dose rate rather than with the dose; additionally, data on shielding effects inside and outside the space craft and its exposure were gained from the different SDEF- and LDEF-missions.