Design of specific hardware to obtain embryos and maintain adult urodele amphibians aboard a space station.

The study of the influence of weightlessness on fertilization and embryonic development of a vertebrate is of importance in the understanding of basic embryogenesis and in the preparation of the future exploration of space. Accordingly, specific hardware was designed to perform experiments on board the MIR space station with an amphibian vertebrate model, taking into account the biological requirements and the multiple constraints of a long-term mission. This paper describes the biological uses and presents the technological specifications of the device developed under CNES management. The hardware was adapted to and is compatible with biological requirements as confirmed by three experiments performed in space on board the orbital MIR station.     

CELSS transportation analysis

Regenerative life support systems based on the use of biological material have been considered for inclusion in manned spacecraft since the early days of the United States space program. These biological life support systems are currently being developed by NASA in the Controlled Ecological Life Support System (CELSS) program. Because of the progress being achieved in the CELSS program, it is time to determine which space missions may profit from use of the developing technology. This paper presents the results of a study that was conducted to estimate where potential transportation cost savings could be anticipated by using CELSS technology for selected future manned space missions.

Six representative missions were selected for study from those included in NASA planning studies. The selected missions ranged from a low Earth orbit mission to those associated with asteroids and a Mars sortie. The crew sizes considered varied from four persons to five thousand. Other study parameters included mission duration and life support closure percentages, with the latter ranging from complete resupply of consumable life support materials to 97% closure of the life support system. The paper presents the analytical study approach and describes the missions and systems considered, together with the benefits derived from CELSS when applicable.     

Structural and functional organisation of regenerated plant protoplasts exposed to microgravity on Biokosmos 9.

Preparatory experiments for the IML-1 mission using plant protoplasts, were flown on a 14-day flight on Biokosmos 9 in September 1989. Thirty-six hours before launch of the biosatellite, protoplasts were isolated from hypocotyl cells of rapeseed (Brassica napus) and suspension cultures of carrot (Daucus carota). Ultrastructural and fluorescence analysis of cell aggregates from these protoplasts, cultured under microgravity conditions, have been performed. In the flight samples as well as in the ground controls, a portion of the total number of protoplasts regenerated cell walls. The processes of cell differentiation and proliferation under micro-g did not differ significantly from those under normal gravity conditions. However, in micro-g differences were observed in the ultrastructure of some organelles such as plastids and mitochondria. There was also an increase in the frequency of the occurrence of folds formed by the plasmalemma together with an increase in the degree of complexity of these folds. In cell cultures developed under micro-g conditions, the calcium content tends to decrease, compared to the ground control. Different aspects of using isolated protoplasts for clarifying the mechanisms of biological effects of microgravity are discussed.     

Particle trajectories in seeds of Lactuca sativa and chromosome aberrations after exposure to cosmic heavy ions on Cosmos Biosatellites 8 and 9.

The potentially specific importance of the heavy ions of the galactic cosmic radiation for radiation protection in manned spaceflight continues to stimulate in situ, i.e., spaceflight experiments to investigate their radiobiological properties. Chromosome aberrations as an expression of a direct assault on the genome are of particular interest in view of cancerogenesis being the primary radiation risk for man in space. In such investigations the establishment of the geometrical correlation between heavy ions' trajectories and the location of radiation sensitive biological substructures is an essential task. The overall qualitative and quantitative precision achieved for the identification of particle trajectories in the order of approximately 10 micrometers as well as the contributing sources of uncertainties are discussed. We describe how this was achieved for seeds of Lactuca sativa as biological test organisms, whose location and orientation had to be derived from contact photographies displaying their outlines and those of the holder plates only. The incidence of chromosome aberrations in cells exposed during the COSMOS 1887 (Biosatellite 8) and the COSMOS 2044 (Biosatellite 9) mission was determined for seeds hit by cosmic heavy ions. In those seeds the incidence of both single and multiple chromosome aberrations was enhanced. The results of the Biosatellite 9 experiment, however, are confounded by spaceflight effects unrelated to the passage of heavy ions.     

基于作战单元任务成功性的可修复备件优化

在分析作战单元任务及备件保障流程的基础上,在考虑装备系统冷备份和热备份的情况下,基于METRIC模型原理,建立了两级保障体制下的作战单元任务成功性评估模型.以备件购置费用为优化目标函数,作战单元任务成功概率为约束条件,运用边际方法给出了备件优化模型的求解方法.最后,通过实例对该方法进行了分析,优化结果符合作战单元备件配置实际情况,从而为部队装备保障指挥人员在作战单元执行任务前科学制定保障方案提供决策支持.     

Observing the earth radiation budget from satellites: Past,present, and a look to the future

Satellite measurements of the radiative exchange between the planet Earth and space have been the objective of many experiments since the beginning of the space age in the late 1950's. The on-going mission of the Earth Radiation Budget (ERB) experiments has been and will be to consider flight hardware, data handling and scientific analysis methods in a single design strategy. Research and development on observational data has produced an analysis model of errors associated with ERB measurement systems on polar satellites. Results show that the variability of reflected solar radiation from changing meteorology dominates measurement uncertainties. As an application, model calculations demonstrate that measurement requirements for the verification of climate models may be satisfied with observations from one polar satellite, provided we have information on diurnal variations of the radiation budget from the ERBE mission.     

Biological life-support systems for Mars mission.

Mars mission like the Lunar base is the first venture to maintain human life beyond earth biosphere. So far, all manned space missions including the longest ones used stocked reserves and can not be considered egress from biosphere. Conventional path proposed by technology for Martian mission LSS is to use physical-chemical approaches proved by the experience of astronautics. But the problem of man living beyond the limits of the earth biosphere can be fundamentally solved by making a closed ecosystem for him. The choice optimum for a Mars mission LSS can be substantiated by comparing the merits and demerits of physical-chemical and biological principles without ruling out possible compromise between them. The work gives comparative analysis of ecological and physical-chemical principles for LSS. Taking into consideration universal significance of ecological problems with artificial LSS as a particular case of their solution, complexity and high cost of large-scale experiments with manned LSS, it would be expedient for these works to have the status of an International Program open to be joined. A program of making artificial biospheres based on preceding experience and analysis of current situation is proposed.     

军用飞机使用可用度仿真论证

使用可用度是军用飞机重要的可靠性维修性保障性RMS(Reliability, Maintainability and Supportability)顶层参数.为了确定合理有效的使用可用度,根据论证阶段的实际情况,综合考虑军用飞机任务需求、初始使用和保障方案以及相似装备的RMS指标等信息,利用Monte-Carlo方法和排队论构建模型.对仿真过程中的任务开始、故障时刻、维修时间的抽样进行了分析,给出了抽样公式.以军用飞机RMS指标和使用保障方案作为仿真输入,反复迭代计算获得一族使用可用度与RMS参数的关系曲线,再综合权衡获得合理的使用可用度和相关RMS指标.给出了可靠性仿真的算法流程和相关的计算公式,并以案例验证了该方法的可行性.仿真模型具有通用性,可根据实际情况修改统计公式,满足不同参数的论证需求.      

Alkylating agent (MNU)-induced mutation in space environment.

In recent years, some contradictory data about the effects of microgravity on radiation-induced biological responses in space experiments have been reported. We prepared a damaged template DNA produced with an alkylating agent (N-methyl-N-nitroso urea; MNU) to measure incorrect base-incorporation during DNA replication in microgravity. We examined whether mutation frequency is affected by microgravity during DNA replication for a DNA template damaged by an alkylating agent. Using an in vitro enzymatic reaction system, DNA synthesis by Taq polymerase or polymerase III was done during a US space shuttle mission (Discovery, STS-91). After the flight, DNA replication and mutation frequencies were measured. We found that there was almost no effect of microgravity on DNA replication and mutation frequency. It is suggested that microgravity might not affect at the stage of substrate incorporation in induced-mutation frequency.     

Effects of rectilinear acceleration, caloric and optokinetic stimulation of human subjects in the Spacelab D-1 mission

A set of vestibular experiments was performed during the course of the German Spacelab D-1 mission from 30 October to 6 November 1985 by a consortium of experimenters from various european countries. Similar to the Spacelab SL-1 mission all of the scientific crew members were theoretically and practically trained for the experiments. Baseline measurements for all tests were collected 113, 86, 44, 30 and 18 days prior to the mission and compared with data taken inflight, on the landing day and the consecutive 7 to 14 days.

The hardware comprised mainly a motordriven accelerating platform, the SPACE SLED, and the vestibular helmet, a multi-purpose instrument in support of a variety of vestibular experiments including air-calorisation of the ears, optokinetic stimulation pattern presentation and optical and nystagmographic recording of eye movements.

Measurements of the threshold for the perception of detection of whole body movement did not reveal any dramatic changes in the 2 measured axes inflight when compared to preflight values. Early postflight values show a significantly elevated threshold for all axes in 3 out of 4 subjects.

The caloric nystagmus, already found during the SL-1 mission, was confirmed on all three tested subjects during the D-1 mission. It's amplitude and in some instances it's direction were influenced by horizontal acceleration on the SLED.

The amplitude of optokinetic nystagmus increased when subjects were allowed to free-float over that seen when subjects were fixed.

Stimulation of the neck receptors by roll movements of the body against the fixated head resulted in illusory object motion to the contralateral side. Torsional movements of the eyes during such neck receptor stimulation was present inflight and postflight, while it had not been observed preflight. Most results point to a reduction of otolithic effects in favour of visual and proprioceptive influences for spatial orientation.     

Clover development during spaceflight: a model system.

The development of legume root nodules was studied as a model system for the examination of gravitational effects on plant root development. In order to examine whether rhizobial association with clover roots can be achieved in microgravity, experiments were performed aboard the KC-135 parabolic aircraft and aboard the sounding rocket mission Consort 3. Binding of rhizobia to roots and the initial stages of root nodule development successfully occurred in microgravity. Seedling germination experiments were performed in the sliding block device, the Materials Dispersion Apparatus, aboard STS-37. When significant hydration of the seeds was achieved, normal rates of germination and seedling development were observed.     

复杂可修系统效能中可信度矩阵的构造方法

复杂可修系统可信度建模对保证效能的定量分析至关重要.本文在考虑不同维修状况的基础上,着重对不同任务剖面内相应任务阶段中武器装备可信度进行了深入的定量分析和综合,进而构造出武器装备的可信度矩阵,用于武器装备的效能分析;文中以某地面车辆为例,进一步论证了武器装备可信度矩阵的工程含义,旨在为合理确定一大类武器装备的效能和可信度指标提供理论依据.     

Planetary protection issues in advance of human exploration of Mars.

Current planetary quarantine considerations focus on robotic missions and attempt a policy of no biological contamination. The presence of humans on Mars, however, will inevitably result in biological contamination and physical alteration of the local environment. The focus of planetary quarantine must therefore shift toward defining and minimizing the inevitable contamination associated with humans. This will involve first determining those areas that will be affected by the presence of a human base, then verifying that these environments do not harbor indigenous life nor provide sites for Earth bacteria to grow. Precursor missions can provide salient information that can make more efficient the planning and design of human exploration missions. In particular, a robotic sample return mission can help to eliminate the concern about returning samples with humans or the return of humans themselves from a planetary quarantine perspective. Without a robotic return the cost of quarantine that would have to be added to a human mission may well exceed the cost of a robotic return mission. Even if the preponderance of scientific evidence argues against the presence of indigenous life, it must be considered as part of any serious planetary quarantine analysis for missions to Mars. If there is life on Mars, the question of human exploration assumes an ethical dimension.     

Application of sunlight and lamps for plant irradiation in space bases.

The radiation sources used for plant growth on a space base must meet the biological requirements for photosynthesis and photomorphogenesis. In addition the sources must be energy and volume efficient, while maintaining the required irradiance levels, spectral, spatial and temporal distribution. These requirements are not easily met, but as the biological and mission requirements are better defined, then specific facility designs can begin to accommodate both the biological requirements and the physical limitations of a space based plant growth system.     

考虑多因素的可修系统任务可靠性分配方法

在实际工程中,系统常常是由串联、并联、旁联和表决等模型混合而成的复杂可修系统,目前此类系统的可靠性分配方法多采用等分分配等方法,得到的分配结果往往误差较大。本文提出了一种考虑维修、故障逻辑等多因素影响的可修系统任务可靠性分配方法。该方法以系统故障率为待分配指标,首先对包含维修影响的故障率进行转换,然后利用考虑故障逻辑的评分分配法进行分配,最后通过备件系数进行修正,获得分配结果。新方法能够为在实际工程中可修系统的任务可靠性分配提供一种简便易行的方法。      

Mission simulation as an approach to develop requirements for automation in Advanced Life Support Systems.

This paper examines mission simulation as an approach to develop requirements for automation and robotics for Advanced Life Support Systems (ALSS). The focus is on requirements and applications for command and control, control and monitoring, situation assessment and response, diagnosis and recovery, adaptive planning and scheduling, and other automation applications in addition to mechanized equipment and robotics applications to reduce the excessive human labor requirements to operate and maintain an ALSS. Based on principles of systems engineering, an approach is proposed to assess requirements for automation and robotics using mission simulation tools. First, the story of a simulated mission is defined in terms of processes with attendant types of resources needed, including options for use of automation and robotic systems. Next, systems dynamics models are used in simulation to reveal the implications for selected resource allocation schemes in terms of resources required to complete operational tasks. The simulations not only help establish ALSS design criteria, but also may offer guidance to ALSS research efforts by identifying gaps in knowledge about procedures and/or biophysical processes. Simulations of a planned one-year mission with 4 crewmembers in a Human Rated Test Facility are presented as an approach to evaluation of mission feasibility and definition of automation and robotics requirements.     

基于有限状态机的交会对接飞行任务规划方法

为实现多约束条件下载人航天器交会对接（RVD）飞行任务的快速、准确规划,提出了一种基于有限状态机（FSM）的飞行任务规划方法。通过飞行过程与有限状态机的映射关系建立图形化任务规划模型,以飞行事件为状态,以测控、光照等约束条件为输入信号,驱动有限状态机的状态转移,进行模型求解,实现交会对接飞行任务的自动规划。以中国神舟十号飞行任务中航天员手控交会对接试验为例,进行了规划验证。经对比,规划计算结果与任务实施结果一致,表明所提方法可以实现交会对接飞行任务的快速、准确规划。