The use of mineral crystals as bio-markers in the search for life on Mars.

Photographs that depict presumed fluvial features on the martian surface have led geologists to hypothesize that water flowed across the early martian terrain. From this, it has been further hypothesized that the surface and atmospheric conditions on early Mars were similar to those on early Earth. Because the oldest fossil evidence of life on Earth dates back to this early period, at least 3.5 billion years ago, the possibility exists that the early Martian environment could have also been conducive to the origin of life. To investigate this possibility, universal signatures or bio-markers indicative of past (or present) biological activity must be identified for use in the search for life on Mars. Several potentially applicable biomarkers have been identified and include: organics (e.g., specific classes of lipids and hopanes), suites of specific inorganic and organic compounds, as well as the isotopic ratios of C, N, and S. Unfortunately, all of these bio-markers may be of biologic or abiotic origin; these origins are often difficult to distinguish. Thus, the discovery of any one of these compounds alone is not a bio-marker. Because minerals produced under biologic control have distinctive crystallographies, morphologies, and isotopic ratios that distinguishable from abiotically produced minerals with the same chemical composition, and are stable through geologic time, we propose the use of minerals resulting from biologically controlled mineralization processes as bio-markers.     

Biological nitrogen fixation under primordial Martian partial pressures of dinitrogen.

Early Earth and early Mars were similar enough such that past geochemical and climatic conditions on Mars may have also been favorable for the origin of life. However, one of the most striking differences between the two planets was the low partial pressure of dinitrogen (pN2) on early Mars (18 mb). On Earth, nitrogen is a key biological element and in many ecosystems the low availability of fixed nitrogen compounds is the main factor limiting growth. Biological fixation of dinitrogen on Earth is a crucial source of fixed nitrogen. Could the low availability of dinitrogen in the primordial Martian atmosphere have prevented the existence, or evolution of Martian microbiota? Azotobacter vinelandii and Azomonas agilis were grown in nitrogen free synthetic medium under various partial pressures of dinitrogen ranging from 780-0 mb (total atmosphere=1 bar). Below 400 mb the biomass, cell number, and growth rate decreased with decreasing pN2. Both microorganisms were capable of growth at a pN2 as low as 5 mb, but no growth was observed at a pN2 < or = 1 mb. The data appear to indicate that biological nitrogen fixation could have occurred on primordial Mars (pN2=18 mb) making it possible for a biotic system to have played a role in the Martian nitrogen cycle. It is possible that nitrogen may have played a key role in the early evolution of life on Mars, and that later a lack of available nitrogen on that planet (currently, pN2=0.2 mb) may have been involved in its subsequent extinction.     

Exobiology and future Mars missions: The search for Mars'' earliest biosphere

The primordial Mars may have possessed a thick carbon dioxide atmosphere, with liquid water common on the surface, similar in many ways to the primordial Earth. During this epoch, billions of years ago, the surface of Mars could have been conducive to the origin of life. It is possible that life evolved on Mars to be later eliminated as the atmospheric pressure dropped. Analysis of the surface of Mars for the traces of this early martian biota could provide many insights into the phenomenon of life and its coupling to planetary evolution.     

Planetary protection issues and the future exploration of Mars.

A primary scientific theme for the Space Exploration Initiative (SEI) is the search for life, extant or extinct, on Mars. Because of this, concerns about Planetary Protection (PP), the prevention of biological cross-contamination between Earth and other planets during solar system exploration missions, have arisen. A recent workshop assessed the necessity for, and impact of, PP requirements on the unmanned and human missions to Mars comprising the SEI. The following ground-rules were adopted: 1) information needed for assessing PP issues must be obtained during the unmanned precursor mission phase prior to human landings; 2) returned Mars samples will be considered biologically hazardous until proven otherwise; 3) deposition of microbes on Mars and exposure of the crew to Martian materials are inevitable when humans land; and, 4) human landings are unlikely until it is demonstrated that there is no harmful effect of Martian materials on terrestrial life forms. These ground-rules dictated the development of a conservative PP strategy for precursor missions. Key features of the proposed strategy include: 1) for prevention of forward contamination, all orbiters will follow Mars Observer PP procedures for assembly, trajectory, and lifetime. All landers will follow Viking PP procedures for assembly, microbial load reduction, and bioshield; and, 2) for prevention of back contamination, all sample return missions will have PP requirements which include fail-safe sample sealing, breaking contact chain with the Martian surface, and containment and quarantine analysis in an Earth-based lab. In addition to deliberating on scientific and technical issues, the workshop made several recommendations for dealing with forward and back contamination concerns from non-scientific perspectives.     

Early martian environments: the Antarctic and other terrestrial analogs.

The comparability of the early environments of Mars and Earth, and the biological evolution which occurred on early earth, motivates serious consideration of the possibility of an early martian biota. Environments which could have contained this early martian life and which may presently contain evidence of this former life include aquatic, ice, soil, and rock habitats. Several analogs of these potential early martian environments, which can provide useful information in searching for extinct life on Mars, are currently available for study on Earth. These terrestrial analogs include the perennially ice-covered lakes and sandstone rocks in the Polar Deserts of Antarctica, surface of snowfields and glaciers, desert soils, geothermal springs, and deep subsurface environments.     

Planetary protection and the search for life beneath the surface of Mars.

The search for traces of extinct and extant life on Mars will be extended to beneath the surface of the planet. Current data from Mars missions suggesting the presence of liquid water early in Mars' history and mathematical modeling of the fate of water on Mars imply that liquid water may exist deep beneath the surface of Mars. This leads to the hypothesis that life may exist deep beneath the Martian surface. One possible scenario to look for life on Mars involves a series of unmanned missions culminating with a manned mission drilling deep into the Martian subsurface (approximately 3Km), collecting samples, and conducting preliminary analyses to select samples for return to earth. This mission must address both forward and back contamination issues, and falls under planetary protection category V. Planetary protection issues to be addressed include provisions stating that the inevitable deposition of earth microbes by humans should be minimized and localized, and that earth microbes and organic material must not contaminate the Martian subsurface. This requires that the drilling equipment be sterilized prior to use. Further, the collection, containment and retrieval of the sample must be conducted such that the crew is protected and that any materials returning to earth are contained (i.e., physically and biologically isolated) and the chain of connection with Mars is broken.     

The search for nitrogen compounds on the surface of Mars

Nitrogen is an essential element for life. Specifically, “fixed nitrogen” (i.e., NH₃, NH₄⁺, NO_x, or N that is chemically bound to either inorganic or organic molecules and is releasable by hydrolysis to NH₃ or NH₄⁺) is the form of nitrogen useful to living organisms. To date no direct analysis of Martian soil nitrogen content, or content of fixed nitrogen compounds has been done. Consequently, the planet's total inventory of nitrogen is unknown. What is known is that the N₂ content of the present-day Martian atmosphere is 0.2 mbar. It has been hypothesized that early in Mars' history (3 to 4 billion years ago) the Martian atmosphere contained much more N₂ than it does today. The values of N₂ proposed for this early Martian atmosphere, however, are not well constrained and range from 3 to 300 mbar of N₂. If the early atmosphere of Mars did contain much more N₂ than it does today the question to be answered is, Where did it go? The two main processes that could have removed it rapidly from the atmosphere include: 1) nonthermal escape of N-atoms to space; and 2) burial within the regolith as nitrates and nitrites. Nitrate will be stable in the highly oxidized surface soil of Mars, and will tend to accumulate in the soil. Such accumulations are observed in certain desert environments on Earth. Some NH₄⁺---N may also be fixed and stabilized in the soil by inclusion as a structural cation in the crystal lattices of certain phyllosilicates replacing K. Analysis of the Martian soil for traces of NO₃⁻ and NH₄⁺ during future missions will supply important information regarding the nitrogen abundance on Mars, its past climate as well as its potential for the evolution of life.     

Network science landers for Mars

The NetLander Mission will deploy four landers to the Martian surface. Each lander includes a network science payload with instrumentation for studying the interior of Mars, the atmosphere and the subsurface, as well as the ionospheric structure and geodesy. The NetLander Mission is the first planetary mission focusing on investigations of the interior of the planet and the large-scale circulation of the atmosphere. A broad consortium of national space agencies and research laboratories will implement the mission. It is managed by CNES (the French Space Agency), with other major players being FMI (the Finnish Meteorological Institute), DLR (the German Space Agency), and other research institutes. According to current plans, the NetLander Mission will be launched in 2005 by means of an Ariane V launch, together with the Mars Sample Return mission. The landers will be separated from the spacecraft and targeted to their locations on the Martian surface several days prior to the spacecraft's arrival at Mars. The landing system employs parachutes and airbags. During the baseline mission of one Martian year, the network payloads will conduct simultaneous seismological, atmospheric, magnetic, ionospheric, geodetic measurements and ground penetrating radar mapping supported by panoramic images. The payloads also include entry phase measurements of the atmospheric vertical structure. The scientific data could be combined with simultaneous observations of the atmosphere and surface of Mars by the Mars Express Orbiter that is expected to be functional during the NetLander Mission's operational phase. Communication between the landers and the Earth would take place via a data relay onboard the Mars Express Orbiter.     

A "cryptic" microbial mat: a new model ecosystem for extant life on Mars.

If life were present on Mars to day, it would face potentially lethal environmental conditions such as a lack of water, frigid temperatures, ultraviolet radiation, and soil oxidants. In addition, the Viking missions did not detect near-surface organic carbon available for assimilation. Autotrophic organisms that lived under a protective layer of sand or gravel would be able to circumvent the ultraviolet radiation and lack of fixed carbon. Two terrestrial photosynthetic near-surface microbial communities have been identified, one in the inter- and supertidal of Laguna Ojo de Liebre (Baja California Sur, Mexico) and one in the acidic gravel near several small geysers in Yellowstone National Park (Wyoming, U.S.A.). Both communities have been studied with respect to their ability to fix carbon under different conditions, including elevated levels of inorganic carbon. Although these sand communities have not been exposed to the entire suite of Martian environmental conditions simultaneously, such communities can provide a useful model ecosystem for a potential extant Martian biota.     

Biological space experiments for the simulation of Martian conditions: UV radiation and Martian soil analogues.

The survivability of resistant terrestrial microbes, bacterial spores of Bacillus subtilis, was investigated in the BIOPAN facility of the European Space Agency onboard of Russian Earth-orbiting FOTON satellites (BIOPAN I -III missions). The spores were exposed to different subsets of the extreme environmental parameters in space (vacuum, extraterrestrial solar UV, shielding by protecting materials like artificial meteorites). The results of the three space experiments confirmed the deleterious effects of extraterrestrial solar UV radiation which, in contrast to the UV radiation reaching the surface of the Earth, also contains the very energy-rich, short wavelength UVB and UVC radiation. Thin layers of clay, rock or meteorite material were shown to be only successful in UV-shielding, if they are in direct contact with the spores. On Mars the UV radiation climate is similar to that of the early Earth before the development of a protective ozone layer in the atmosphere by the appearance of the first aerobic photosynthetic bacteria. The interference of Martian soil components and the intense and nearly unfiltered Martian solar UV radiation with spores of B. subtilis will be tested with a new BIOPAN experiment, MARSTOX. Different types of Mars soil analogues will be used to determine on one hand their potential toxicity alone or in combination with solar UV (phototoxicity) and on the other hand their UV protection capability. Two sets of samples will be placed under different cut-off filters used to simulate the UV radiation climate of Mars and Earth. After exposure in space the survival of and mutation induction in the spores will be analyzed at the DLR, together with parallel samples from the corresponding ground control experiment performed in the laboratory. This experiment will provide new insights into the principal limits of life and its adaptation to environmental extremes on Earth or other planets which and will also have implications for the potential for the evolution and distribution of life.     

Development and research program for a soil-based bioregenerative agriculture system to feed a four person crew at a Mars base.

For humans to survive during long-term missions on the Martian surface, bioregenerative life support systems including food production will decrease requirements for launch of Earth supplies, and increase mission safety. It is proposed that the development of "modular biospheres"--closed system units that can be air-locked together and which contain soil-based bioregenerative agriculture, horticulture, with a wetland wastewater treatment system is an approach for Mars habitation scenarios. Based on previous work done in long-term life support at Biosphere 2 and other closed ecological systems, this consortium proposes a research and development program called Mars On Earth(TM) which will simulate a life support system designed for a four person crew. The structure will consist of 6 x 110 square meter modular agricultural units designed to produce a nutritionally adequate diet for 4 people, recycling all air, water and waste, while utilizing a soil created by the organic enrichment and modification of Mars simulant soils. Further research needs are discussed, such as determining optimal light levels for growth of the necessary range of crops, energy trade-offs for agriculture (e.g. light intensity vs. required area), capabilities of Martian soils and their need for enrichment and elimination of oxides, strategies for use of human waste products, and maintaining atmospheric balance between people, plants and soils.     

火星大气模型参数对MSL气动特性的影响

火星大气与地球大气截然不同,飞行器在进入火星时气动特性不同于地球再入. 大气模型的差异主要表现为气体组份、密度和温度等物理参数. 针对火星进入器MSL在进入-下降-着陆过程中的高超声速进入段,利用三维并行程序求解耦合真实气体模型的流体动力学Navier-Stokes方程,分析MSL进入火星大气时大气模型参数对进入器气动特性的影响. 结果表明,通过与海盗号飞行数据的对比,验证了所采用的火星气体模型和计算方法,且其与NASA的 LAURA代码气动特性计算结果也较为一致;大气模型气体性质,即CO₂环境对进入器阻力系数和俯仰力矩系数影响较大,利用空气得到的计算和实验数据必须考虑CO₂效应;密度增大促进了化学非平衡效应,但对进入器气动特性基本没有影响;温度升高大大增强了化学非平衡效应,而对进入器气动特性影响较小.      

The implantation of life on Mars: feasibility and motivation.

Environmental conditions on Mars are extremely hostile, and would be destructive to any organisms which might arrive there unprotected to-day. However, it is a biocompatible planet. Its unalterable astrophysical parameters would allow the maintenance of a much thicker, warmer carbon dioxide atmosphere than that which currently exists. Though very cold (averaging about -60 degrees C), highly oxidizing and desiccated, Mars may possess substantial quantities of the materials needed to support life--in particular, water and carbon dioxide. A general scenario for implanting life on Mars would include three main phases: (1) robotic and human exploration to determine whether sufficiently large and accessible volatile inventories are available; (2) planetary engineering designed to warm the planet, release liquid water and produce a thick carbon dioxide atmosphere; and (3) if no indigenous Martian organisms emerge as liquid water becomes available, a program of biological engineering designed to construct and implant pioneering microbial communities able to proliferate in the newly clement, though still anaerobic, Martian environment. The process of establishing an ecosystem, or biosphere, on a lifeless planet is best termed 'ecopoiesis.' This new word, derived from Greek, means 'the making of an abode for life.' It is by no means clear whether ecopoiesis on Mars is scientifically possible or technologically achievable. Thus we urge that it be one of the objectives of space research during the next century to assess the feasibility of ecopoiesis on Mars.     

Studies in the search for life on Mars.

The ability of living organisms to survive extraterrestrial conditions has implications for the origins of life in the solar system. We have therefore studied the survival of viruses, bacteria, yeast, and fungi under simulated Martian conditions. The environment on Mars was simulated by low temperature, proton irradiation, ultraviolet irradiation, and simulated Martian atmosphere (CO2 95.46%, N2 2.7%, water vapor 0.03%) in a special cryostat. After exposure to these conditions, tobacco mosaic virus and spores of Bacillus, Aspergillus, Clostridium, and some species of coccus showed significant survival.     

Possible complex organic compounds on Mars.

It is suggested that primitive Mars had somehow similar environments as primitive Earth. If life was born on the primitive earth using organic compounds which were produced from the early Earth environment, the same types of organic compounds were also formed on primitive Mars. Such organic compounds might have been preserved on Mars still now. We are studying possible organic formation on primitive and present Mars. A gaseous mixture of CO2, CO, N2 and H2O with various mixing ratios were irradiated with high energy protons (major components of cosmic rays). Hydrogen cyanide and formaldehyde were detected among volatile products, and yellow-brown-colored water-soluble non-volatile substances were produced, which gave amino acids after acid-hydrolysis. Major part of "amino acid precursors" were not simple molecules like aminonitriles, but complex compounds which eluted earlier than free amino acids in cation-exchange HPLC. These organic compounds should be major targets in the future Mars mission. Strategy for the detection of the complex organics on Mars will be discussed.     

Possible applications of aquatic bioregenerative life support modules for food production in a Martian base.

Water is the essential precondition of life in general and also for the establishment of a Martian base suitable for long duration stays of humans. It is not yet proven if there is indeed a "frozen ocean" under the surface of Mars but if this could be verified it would open innovative aspects for the construction of bioregenerative life support systems (BLSS). In a general concept higher plants will play the predominant role in a Martian BLSS. It is not clear, however, how these will grow and bring seed in reduced gravity and there may be differences in the productivity in comparison to Earth conditions. Therefore, organisms which are already adapted to low gravity conditions, namely non-gravitropic aquatic plants and also aquatic animals may be used to enhance the functionality of the Martian BLSS as a whole. It has been shown already with the so-called C.E.B.A.S. MINIMODULE in the STS-89 and STS-90 spaceshuttle missions that the water plant Ceratophyllum demersum has an undisturbed and high biomass production under space conditions. Moreover, the teleost fish species Xiphophorus helleri adapted easily to the micro-g environment and maintained its normal reproductive functions. Based on this findings a possible scenario is presented in which aquatic plant production modules and combined animal-plant production systems may be used for human food production and water and air regeneration in a Martian base.     

The magnetic fields of Mercury,Venus and Mars

Just as clearly as Mariner 10 established that Mercury has an intrinsic magnetic field, the Pioneer Venus orbiter has established that Venus has no significant intrinsic field. This is perhaps the opposite of what might be expected. Mercury, a small planet might be expected to cool rapidly and its internal dynamo to cease, while Venus, which is almost as large as the Earth, should not have lost much heat. On the contrary the source of energy of the Mercury dynamo appears to be extant whereas that of Venus appears to be extinct.The existence of a Martian magnetic field is controversial. No unambiguous signature of a Martian magnetic field has been reported. If the field on the nightside of Mars is of planetary rather than solar origin the Russian Mars spacecraft observations indicate the Martian dipole lies near the planetary equator rather than its pole.     

Planetary protection issues in advance of human exploration of Mars.

Current planetary quarantine considerations focus on robotic missions and attempt a policy of no biological contamination. The presence of humans on Mars, however, will inevitably result in biological contamination and physical alteration of the local environment. The focus of planetary quarantine must therefore shift toward defining and minimizing the inevitable contamination associated with humans. This will involve first determining those areas that will be affected by the presence of a human base, then verifying that these environments do not harbor indigenous life nor provide sites for Earth bacteria to grow. Precursor missions can provide salient information that can make more efficient the planning and design of human exploration missions. In particular, a robotic sample return mission can help to eliminate the concern about returning samples with humans or the return of humans themselves from a planetary quarantine perspective. Without a robotic return the cost of quarantine that would have to be added to a human mission may well exceed the cost of a robotic return mission. Even if the preponderance of scientific evidence argues against the presence of indigenous life, it must be considered as part of any serious planetary quarantine analysis for missions to Mars. If there is life on Mars, the question of human exploration assumes an ethical dimension.     

Phosphorus as a potential guide in the search for extinct life on Mars.

In contrast to the search for extant organisms, the quest for fossil remains of life on Mars need not be guided by the presence of water and organic compounds on the present surface. An appropriate tracer might be the element phosphorus which is a common constituent of living systems. Utilizing terrestrial analogues, it should preferentially exist in the form of sedimentary calcium phosphate (phosphorites), which would have readily resisted changing conditions on Mars. Moreover, higher ratios of P/Th in phosphorites in comparison to calcium phosphates from magmatic rocks give us the possibility to distinguish them from inorganically formed phosphorus deposits at or close to the Martian surface. Identification of anomalous phosphorus enrichments by remote sensing or in situ analysis could be promising approaches for selecting areas preferentially composed of rocks with remains of extinct life.