Characterizing parameters of Jatropha curcas cell cultures for microgravity studies

Jatropha (Jatropha curcas) is a tropical perennial species identified as a potential biofuel crop. The oil is of excellent quality and it has been successfully tested as biodiesel and in jet fuel mixes. However, studies on breeding and genetic improvement of jatropha are limited. Space offers a unique environment for experiments aiming at the assessment of mutations and differential gene expression of crops and in vitro cultures of plants are convenient for studies of genetic variation as affected by microgravity. However, before microgravity studies can be successfully performed, pre-flight experiments are necessary to characterize plant material and validate flight hardware environmental conditions. Such preliminary studies set the ground for subsequent spaceflight experiments. The objectives of this study were to compare the in vitro growth of cultures from three explant sources (cotyledon, leaf, and stem sections) of three jatropha accessions (Brazil, India, and Tanzania) outside and inside the petriGAP, a modified group activation pack (GAP) flight hardware to fit petri dishes. In vitro jatropha cell cultures were established in petri dishes containing a modified MS medium and maintained in a plant growth chamber at 25 ± 2 °C in the dark. Parameters evaluated were surface area of the explant tissue (A), fresh weight (FW), and dry weight (DW) for a period of 12 weeks. Growth was observed for cultures from all accessions at week 12, including subsequent plantlet regeneration. For all accessions differences in A, FW and DW were observed for inside vs. outside the PetriGAPs. Growth parameters were affected by accession (genotype), explant type, and environment. The type of explant influenced the type of cell growth and subsequent plantlet regeneration capacity. However, overall cell growth showed no abnormalities. The present study demonstrated that jatropha in vitro cell cultures are suitable for growth inside PetriGAPs for a period of 12 weeks. The parameters evaluated in this study provide the basic ground work and pre-flight assessment needed to justify a model for microgravity studies with jatropha in vitro cell cultures. Future studies should focus on results of experiments performed with jatropha in vitro cultures in microgravity.     

Cosmic ray detection on the Foton-M2 satellite by a track etch detector stack

In the frame of the European Space Agency (ESA) project called “Biology and Physics in Space”, the returning satellite, Foton-M2, carried an open-to-space exposure platform outside of the satellite body, called as BIOPAN-5, loaded with exo-biological experiments and facilities for radiation dosimetry (RADO). One of the RADO experiments was dedicated to the detection of the primary galactic cosmic rays (GCR) and secondary neutrons by a track etch detector stack. The daily absorbed dose (D) and dose equivalent (H) were calculated from the experimental LET spectra (LET > 10 keV/μm). Under a shielding of ∼2.8 g/cm² the averaged H was found to be 658 ± 8 μSv/d, with a quality factor (Q) of 6.2 ± 1.2. The LET spectra showed a local peak at ∼105 keV/μm suggesting that the majority of tracks were created by trapped protons as it has been predicted by calculations. The low LET dose of the cosmic radiation was determined by 4 TLD stacks, and the total dose was found to be 795 ± 14 μSv/d.     

Estimation of the Love and Shida numbers: h2, l2 using SLR data for the low satellites

In this paper we present results for the global elastic parameters: Love number h₂ and Shida number l₂ derived from the analysis of Satellite Laser Ranging (SLR) data. SLR data for the two low satellites STELLA (H = 800 km) and STARLETTE (H = 810 km) observed during 2.5 years from January 3, 2005 until July 1, 2007 with 18 globally distributed ground stations were analyzed. The analysis was done separately for the two satellites. We do a sequential analysis and study the stability and convergence of the estimates as a function of length of the data set used.     

Heating heavy ions in the polar corona by collisionless shocks: A one-dimensional simulation

Recently a new model for explaining the observations of preferential heating of heavy ions in the polar solar corona was proposed ( and ). In that model the ion energization mechanism is the ion reflection off supercritical quasi-perpendicular collisionless shocks in the corona and the subsequent acceleration by the motional electric field E = −V × B/c. The mechanism of heavy ion reflection is based on ion gyration in the magnetic overshoot of the shock. The acceleration due to the motional electric field is perpendicular to the magnetic field, giving rise to large temperature anisotropy with T_⊥ ? T_∥, in agreement with SoHO observations. Such a model is tested here by means of a one dimensional test particle simulation where ions are launched toward electric and magnetic profiles representing the shock transition. We study the dynamics of O⁵⁺, as representative of coronal heavy ions for Alfvénic Mach numbers of 2–4, as appropriate to solar corona. It is found that O⁵⁺ ions are easily reflected and gain more than mass proportional energy with respect to protons.     

Expression of p53-regulated genes in human cultured lymphoblastoid TSCE5 and WTK1 cell lines after spaceflight in a frozen state

The 53 kDa tumor suppressor protein p53 is generally thought to contribute to the genetic stability of cells and to protect cells from DNA damage through the activity of p53-centered signal transduction pathways. To clarify the effect of space radiation on the expression of p53-dependent regulated genes, gene expression profiles were compared between two human cultured lymphoblastoid cell lines: one line (TSCE5) has a wild-type p53 gene status, and the other line (WTK1) has a mutated p53 gene status. Frozen human lymphoblastoid cells were stored in a freezer in the International Space Station (ISS) for 133 days. Gene expression was analyzed using DNA chips after culturing the space samples for 6 h on the ground after their return from space. Ground control samples were also cultured for 6 h after being stored in a frozen state on the ground for the same time period that the frozen cells were in space. p53-Dependent gene expression was calculated from the ratio of the gene expression values in wild-type p53 cells and in mutated p53 cells. The expression of 50 p53-dependent genes was up-regulated, and the expression of 94 p53-dependent genes was down-regulated after spaceflight. These expression data identified genes which could be useful in advancing studies in basic space radiation biology. The biological meaning of these results is discussed from the aspect of gene functions in the up- and down-regulated genes after exposure to low doses of space radiation.     

Estimation of the elastic Earth parameters (h2, l2) using SLR data

We present results for the global elastic parameters h₂ and l₂ derived from the analysis of Satellite Laser Ranging (SLR) data. SLR data for the two satellites LAGEOS 1 and LAGEOS 2 observed during 2.5 years from January 3, 2005 until July 1, 2007 with 18 globally distributed ground stations were analysed using different approaches. The analysis was done separately for the two satellites and approaches to estimate the two elastic parameters independently and together were performed. We do a sequential analysis and study the stability of the estimates as a function of length of the data set used. The adjusted final values for h₂ equal to 0.6151 ± 0.0008 and 0.6152 ± 0.0008, and those for l₂ equal to 0.0886 ± 0.0003 and 0.0881 ± 0.0003 for LAGEOS 1 and LAGEOS 2 tracking data are compared to other independently derived estimates. These parameters and their errors achieve stability at about the 24 and 27 month time interval for h₂ and l₂, respectively.     

XMM-Newton observations across the Cygnus Loop from northeastern rim to southwestern rim

We have observed the Cygnus Loop from the northeast (NE) to the southwest (SW) with XMM-Newton. We extracted spectra from 3′-spaced annular regions across the Loop and fitted them either with a one-kT_e-component non-equilibrium ionization (NEI) model or with two-kT_e-component NEI model. We found that the two-kT_e-component model yields significantly better fits in almost all the spectra than the one-kT_e-component model. Judging from the abundances, the high-kT_e-component in the two-temperature model must be fossil ejecta while the low-kT_e-component comes from the swept-up interstellar medium (ISM). The distributions of Ne, Mg, Si, and S for fossil ejecta appear to retain the onion-skin structure at the time of a supernova explosion, suggesting that significant overturning of the ejecta has not occurred yet. Comparing the relative abundances of fossil ejecta estimated for the entire Cygnus Loop with those from theoretical calculations for Type-II SN, the mass of the progenitor star is likely to be ∼13 M_⊙. The trends of the radial profiles of kT_e and emission integral for the swept-up ISM are adequately described by the Sedov model, suggesting that the swept-up ISM is concentrated in a shell-like structure. Comparing our data with the Sedov model, we found the ambient medium density to be ∼0.7 cm⁻³. Then, we estimated the total mass of the swept-up ISM and the age of the remnant to be ∼130 M_⊙ and 13,000 years, respectively. Note that if the explosion occurred within a stellar wind cavity, then the density in the cavity, the total swept-up mass in the cavity, and the age of the remnant are estimated to be ∼0.14 cm⁻³, ∼25 M_⊙, and ∼10,000 years, respectively.     

Compact frequency standard using atoms trapped on a chip

We present a compact atomic frequency standard based on the interrogation of magnetically trapped ⁸⁷Rb atoms. Two photons, in the microwave and radiofrequency domain excite the atomic transition. At a magnetic field of 3.23 G this transition from ∣F = 1, m_F = −1〉 to ∣F = 2, m_F = 1〉 is 1st order insensitive to magnetic field variations. Long Ramsey interrogation times can thus be achieved, leading to a projected stability in the low 10⁻¹³ at 1 s. This makes this device a viable alternative to LITE and HORACE as a good candidate for replacing or complementing the rubidium frequency standards and passive hydrogen masers already on board of the GPS, GLONASS, and GALILEO satellites. Here we present preliminary results. We use an atom chip to cool and trap the atoms. A coplanar waveguide is integrated to the chip to carry the Ramsey interrogation signal, making the physics package potentially as small as (5 cm)³. We describe the experimental apparatus and show preliminary Ramsey fringes of 1.25 Hz linewidth. We also show a preliminary frequency stability σ_y = 1.5 × 10⁻¹²τ^−1/2 for 10 < τ < 10³ s. This represents one order of magnitude improvement with respect to previous experiments.     

Efficacy of oxygen-supplying capacity of Azolla in a controlled life support system

Azolla shows high growth and propagation rates, strong photosynthetic O₂-releasing ability and high nutritional value. It is suitable as a salad vegetable and can be cultured on a multi-layered wet bed. Hence, it possesses potential as a fresh vegetable, and to release O₂ and absorb CO₂ in a Controlled Ecological Life Support System in space. In this study, we investigated the O₂-providing characteristics of Azolla in a closed chamber under manned, controlled conditions to lay a foundation for use of Azolla as a biological component in ground simulation experiments for space applications. A closed test chamber, representing a Controlled Ecological Life Support System including an Azolla wet-culture device, was built to measure the changes in atmospheric O₂ and CO₂ concentrations inside the chamber in the presence of coexisting Azolla, fish and men. The amount of O₂ consumed by fish was 0.0805–0.0831 L kg⁻¹ h⁻¹ and the level of CO₂ emission was 0.0705–0.0736 L kg⁻¹ h⁻¹; O₂ consumption by the two trial volunteers was 19.71 L h⁻¹ and the volume of respiration-released CO₂ was 18.90 L h⁻¹. Under 7000–8000 Lx artificial light and Azolla wet-culture conditions, human and fish respiration and Azolla photosynthesis were complementary, thus the atmospheric O₂ and CO₂ concentrations inside chamber were maintained in equilibrium. The increase in atmospheric CO₂ concentration in the closed chamber enhanced the net photosynthesis efficiency of the Azolla colony. This study showed that Azolla has strong photosynthetic O₂-releasing ability, which equilibrates the O₂ and CO₂ concentrations inside the chamber in favor of human survival and verifies the potential of Azolla for space applications.     

Jovian periodicities (∼10 h, ∼40 min) on Ulysses’ Distant Jupiter Encounter observations around the Halloween CIR events

We analyzed data from four different instruments (HI-SCALE, URAP, SWOOPS, VHM/FGM) onboard Ulysses spacecraft (s/c) and we searched for possible evidence of Jovian emissions when the s/c approached Jupiter during the times of Halloween events (closest time approach/position to Jupiter: February 5, 2004/R = 1683 R_J,θ = ∼49°). In particular, we analyzed extensively the low energy ion measurements obtained by the HI-SCALE experiment in order to examine whether low energy ion/electron emissions show a symmetry, and whether they are observed at north high latitudes upstream from the jovian bow shock, as is known to occur in the region upstream from the south bow shock as well ( Marhavilas et al., 2001). We studied the period from October 2003 to March 2004, as Ulysses moved at distances 0.8–1.2 AU from the planet at north Jovicentric latitudes <75°, and we present here an example of characteristic Jovian periodicities in the measurements around a CIR observed by Ulysses on days ∼348–349/2003 (R = 1894 R_J,θ = 72°). We show that Ulysses observed low energy ion (∼0.055–4.7 MeV) and electron (>∼40 keV) flux and/or spectral modulation with the Jupiter rotation period (∼10 h) as well as variations with the same period in solar wind parameters, radio and magnetic field directional data. In addition, characteristic strong ∼40 min periodic variations were found superimposed on the ∼10 h ion spectral modulation. Both the ∼10 h and ∼40 min ion periodicities in HI-SCALE measurements were present in several cases during the whole period examined (October 2003 to March 2004) and were found to be more evident during some special conditions, for instance during enhanced fluxes around the start (forward shock) and the end (reverse shock) of CIRs. We infer that the Jovian magnetosphere was triggered by the impact of the CIRs, after the Halloween events, and it was (a) a principal source of forward and reverse shock-associated ion flux structures and (b) the cause of generation of ∼10 h quasi-periodic magnetic field and plasma modulation observed by Ulysses at those times.     

Change in refractivity of the atmosphere and large variation in TEC associated with some earthquakes,observed from GPS receiver

The present study reports the analysis of GPS based TEC for our station Surat (21.16°N, 72.78°E) located at the northern crest of equatorial anomaly region in India at times close to some earthquake events (M ? 5) during the year 2009 in India and its neighbouring regions. The TEC data used in the study are obtained from GPS Ionospheric Scintillation and TEC Monitoring (GISTM) system. The TEC data has been analysed corresponding to 11 earthquakes in low solar activity period and quiet geomagnetic condition. We found that, out of 11 cases of earthquakes (M > 5) there were seven cases in which enhancement in TEC occurred on earthquake day and in other four cases there was depletion in TEC on earthquake day. The variation in refractivity prior to earthquake was significant for the cases in which the epicentre lied within a distance of 600 km from the receiving station. By looking into the features on temporal enhancement and depletion of TEC a prediction was made 3–2 days prior to an earthquake (on 28 October 2009 in Bhuj – India). The paper includes a brief discussion on the method of potentially identifying an impending earthquake from ionospheric data.     

HORACE: A compact cold atom clock for Galileo

HORACE (HOrloge à Refroidissement d’Atomes en Cellule = clock based on atoms cooled from vapour cell) is a compact cold caesium atom clock developed in SYRTE at Paris Observatory. This clock can operate both on ground and in microgravity environment. Design of HORACE is based on isotropic light cooling, allowing performing the whole clock sequence (cooling, atomic preparation, Ramsey interrogation and detection) at the same place. Compared to more conventional cold atom clocks such as atomic fountains, the use of isotropic light cooling simplifies both the optical part of the setup and the detection sequence, and leads to a drastic size reduction of the physics package. Very good short-term performances have been demonstrated at SYRTE since relative frequency instability of 2.2 × 10⁻¹³ τ^−1/2 has been obtained. Optimization of the long term stability is still under progress and current results show relative frequency instability around 3 × 10⁻¹⁵ in 10⁴ s of integration. With these performances, HORACE appears as a good candidate both for Galileo’s ground segment clock and for onboard Galileo clock.     

A biological method of including mineralized human liquid and solid wastes into the mass exchange of bio-technical life support systems

The main obstacle to using mineralized human solid and liquid wastes as a source of mineral elements for plants cultivated in bio-technical life support systems (BLSS) is that they contain NaCl. The purpose of this study is to determine whether mineralized human wastes can be used to prepare the nutrient solution for long-duration conveyor cultivation of uneven-aged wheat and Salicornia europaea L. plant community. Human solid and liquid wastes were mineralized by the method of “wet incineration” developed by Yu. Kudenko. They served as a basis for preparing the solutions that were used for conveyor-type cultivation of wheat community represented by 5 age groups, planted with a time interval of 14 days. Wheat was cultivated hydroponically on expanded clay particles. To reduce salt content of the nutrient solution, every two weeks, after wheat was harvested, 12 L of solution was removed from the wheat irrigation tank and used for Salicornia europaea cultivation in water culture in a conveyor mode. The Salicornia community was represented by 2 age groups, planted with a time interval of 14 days. As some portion of the nutrient solution used for wheat cultivation was regularly removed, sodium concentration in the wheat irrigation solution did not exceed 400 mg/L, and mineral elements contained in the removed portion were used for Salicornia cultivation. The experiment lasted 4 months. The total wheat biomass productivity averaged 30.1 g · m⁻² · day⁻¹, and the harvest index amounted to 36.8%. The average productivity of Salicornia edible biomass on a dry weight basis was 39.3 g · m⁻² · day⁻¹, and its aboveground mass contained at least 20% of NaCl. Thus, the proposed technology of cultivation of wheat and halophyte plant community enables using mineralized human wastes as a basis for preparing nutrient solutions and including NaCl in the mass exchange of the BLSS; moreover, humans are supplied with additional amounts of leafy vegetables.     

Nusselt number for convection driven by tidal and radiogenic heating

The ‘traditional’ formulas giving the Nusselt number Nu as a function of Rayleigh number Ra cannot be used for low and moderate values of Ra. Moreover, the recent progress in 3D numerical modeling makes possible to determine the Nusselt number Nu as a function of Rayleigh number Ra for convection driven by radiogenic and tidal heating. We found that for low and moderate Ra: Nu(Ra) = ε(Ra+ξ)^λ where λ depends on rheology and boundary conditions, ε depends only on the mode of heating, and ξ = ε^−1/λ · Nu(Ra) makes possible to develop a parameterized theory of convection in medium size icy satellites. We also indicate some differences between convection driven by tidal and radiogenic heating and convection driven exclusively by radiogenic heating.     

Different mechanisms controlling interannual phytoplankton variation in the South China Sea and the western North Pacific subtropical gyre: A satellite study

A time series of remotely-sensed chlorophyll a (chl a) in 1997–2010 was evaluated to determine mechanisms of phytoplankton variation in recent decade in the South China Sea (SCS) and the western North Pacific subtropical gyre (WNPSG). Satellite-derived sea surface temperature (SST) and aerosol optical thickness (AOT) were used as proxies for vertical nutrient supply and atmospheric aerosol, respectively. Chl a in the WNPSG was not significantly correlated with SST (r = 0.18, p > 0.05), but was with AOT (r = 0.31, p < 0.05), indicating the chl a was influenced by atmospheric deposition. Chl a in the SCS was negatively correlated with SST (r = −0.60, p < 0.05) and was positively with AOT (r = 0.20, p < 0.05). The correlation between AOT and chl a in the SCS does not reflect a major contribution from atmospheric deposition to chl a; instead, the relationship resulted from concurrence of the peaks of AOT and wind speed, which drive water mixing and nutrient supply. Consequently, chl a in the SCS would be regulated primarily by the nutrient supply from deep waters. Because SST was controlled by the ENSO teleconnection in the SCS, the chl a was coupled with ENSO events. The present study demonstrated that interannual phytoplankton variation could be controlled by different factors even in neighboring oligotrophic regions.     

Goddard Robotic Telescope – Optical follow-up of GRBs and coordinated observations of AGNs

Since it is not possible to predict when a Gamma-Ray Burst (GRB) will occur or when Active Galactic Nucleus (AGN) flaring activity starts, follow-up/monitoring ground telescopes must be located as uniformly as possible all over the world in order to collect data simultaneously with Fermi and Swift detections. However, there is a distinct gap in follow-up coverage of telescopes in the eastern U.S. region based on the operations of Swift. Motivated by this fact, we have constructed a 14″ fully automated optical robotic telescope, Goddard Robotic Telescope (GRT), at the Goddard Geophysical and Astronomical Observatory. The aims of our robotic telescope are (1) to follow-up Swift/Fermi GRBs and (2) to perform the coordinated optical observations of Fermi Large Area Telescope (LAT) AGN. Our telescope system consists of off-the-shelf hardware. With the focal reducer, we are able to match the field of view of Swift narrow instruments (20′ × 20′). We started scientific observations in mid-November 2008 and GRT has been fully remotely operated since August 2009. The 3σ upper limit in a 30 s exposure in the R filter is ∼15.4 mag; however, we can reach to ∼18 mag in a 600 s exposures. Due to the weather condition at the telescope site, our observing efficiency is 30–40% on average.     

Depth dose distribution measurement on the Foton-M2 bio-satellite by TLD technique

In the framework of “Biology and Physics in Space” project of the European Space Agency (ESA), a returning satellite, Foton-M2, carried an open-to-space sample holder outside of the satellite body, called as BIOPAN-5, loaded with exo-biological experiments and dosemeters for RAdiation DOsimetry (RADO). One of the RADO experiments (Teflon – TLD) was dedicated to dose distribution measurements of the cosmic radiation by thermo-luminescent (TL) technique. It was found that the maximum surface absorbed dose rate, averaged over the first ∼8 mg/cm² thickness, was ∼2 Gy/d and showed a location dependence due the shading effect of the satellite construction elements. The dose rate decreased nearly by 3 orders of magnitude below 500 mg/cm².     

Rotational constants of linear and/or bent Cn+1H and CnN(n = 1–6): A DFT study

The geometries, dipole moments, and rotational constants for the linear and/or bent cations, C_n+1H⁺ and C_nN⁺(n = 1–6), were studied by the B3LYP method with the modest basis sets. For C_nH⁺(n = odd; 3, 5, 7) and C_nN⁺(n = even; 2, 4, 6), the theoretical rotational constants (B_es) of closed-shell singlet C₃H⁺, C₅H⁺, C₇H⁺, CCN⁺, C₄N⁺, and C₆N⁺ were calculated to be about 11,244, 2420, 885.2, 11,970, 2439, and 880.8 MHz, respectively. By contrast, the triplets are stable than the corresponding singlets for C_nH⁺(n = odd; 2, 4, 6) and C_nN⁺(n = even; 3, 5) except CN⁺.     

Studies of the geocenter motion using 16-years DORIS data

An accuracy of geocenter motion estimation is strongly dependent on the geodetic network size and stations distribution over the Earth’s surface. From this point of view DORIS system has an advantage, as its ground network of beacons consists of more than 50 sites, equally distributed over the Earth’s surface. Aiming to study variations of the geocenter movements, the results of DORIS data analysis for the time span 1993.0–2009.0 (inawd06.snx series), performed at the Analysis Centre of the Institute of astronomy of the Russian Academy of Sciences, have been used. DORIS data processing was made with GIPSY/OASIS II software, developed by Jet Propulsion Laboratory and modified for DORIS data processing by Institute Géographique National. Standard deviations of stations coordinates are estimated at the level 0.5–4.0 cm (internal consistency), depending on the number of satellites used in the solution. RMS of estimated components of the DORIS satellites orbits, compared with the solutions of other IDS analysis centres, do not exceed 1–2 cm. Weekly solutions for coordinates have been transformed from free network solutions (inawd06.snx series) to a well defined terrestrial reference frame ITRF2005 with the use of seven parameters of Helmert transformation, which were examined with a view to study variations of the geocenter movements (ina05wd01.geoc time series). In order to estimate linear trend, amplitudes, periods and phases of geocenter variation a method of linear regression was applied. The evaluated amplitudes of annual variations are of the order of 5–7 mm for X and Y components and 27–29 mm for Z component. Semi-annual amplitudes are also noticeable in all components (1–34 mm for X, Y and Z components). Secular trends in the DORIS geocenter coordinates are: −1.2, −0.1 and −0.3 mm/year for X, Y and Z directions respectively.     

Solar cycle and latitude dependence of high-beta suprathermal plasma conditions in interplanetary space between 1.3 and 5.4 AU

The analysis of energetic particles and magnetic field measurements from the Ulysses spacecraft has shown that in a series of events, the energy density contained in the suprathermal tail particle distribution is comparable to or larger than that of the magnetic field, creating conditions of high-beta plasma. In this work we analyze periods of high-beta suprathermal plasma occurrences (β_ep > 1) in interplanetary space, using the ratio (β_ep) of the energetic particle (20 keV to ∼5 MeV) and magnetic field energy densities from measurements covering the entire Ulysses mission lifetime (1990–2009) in order to reveal new or to reconfirm some recently defined interesting characteristics. The main key-results of the work are summarized as follows: (i) we verify that high-beta events are detected within well identified regions corresponding mainly to the vicinity of shock surfaces and magnetic structures, and associated with energetic particle intensity enhancements due to (a) reacceleration at shock-fronts and (b) unusually large magnetic field depressions. (ii) We define three considerable features for the high-beta events, concentrated on the next points: (a) there is an appreciable solar-activity influence on the high-beta events, during the maximum and middle solar-cycle phase, (b) the annual peak magnitude and the number of occurrences of high events are well correlated with the sunspot number, (c) the high-beta suprathermal plasma events present a spatial distribution in heliographic latitudes (HL) up to ∼±80°, and a specific important concentration on the low (−25° ? HL < −6°, 6° < HL ? 25°) and median (−45° ? HL < −25°, 25° < HL ? 45°) latitudes. We also reconfirm by a statistical analysis the results of Marhavilas and Sarris (2011), that the high-beta suprathermal plasma (β_ep > 1) events are characterized by a very large parameter β_ep (up to 1732.5), a great total duration (406 days) and a large percentage of the Ulysses-mission lifetime (which is equal to 6.34% of the total duration with usable measurements, and 11.3% of the duration in presence of suprathermal particles events).