Influence of ionic inorganic solutes on self-assembly and polymerization processes related to early forms of life: implications for a prebiotic aqueous medium

A commonly accepted view is that life began in a marine environment, which would imply the presence of inorganic ions such as Na+, Cl-, Mg2+, Ca2+, and Fe2+. We have investigated two processes relevant to the origin of life--membrane self-assembly and RNA polymerization--and established that both are adversely affected by ionic solute concentrations much lower than those of contemporary oceans. In particular, monocarboxylic acid vesicles, which are plausible models of primitive membrane systems, are completely disrupted by low concentrations of divalent cations, such as magnesium and calcium, and by high sodium chloride concentrations as well. Similarly, a nonenzymatic, nontemplated polymerization of activated RNA monomers in ice/eutectic phases (in a solution of low initial ionic strength) yields oligomers with > 80% of the original monomers incorporated, but polymerization in initially higher ionic strength aqueous solutions is markedly inhibited. These observations suggest that cellular life may not have begun in a marine environment because the abundance of ionic inorganic solutes would have significantly inhibited the chemical and physical processes that lead to self-assembly of more complex molecular systems.     

Understanding strategic decision making in the context of international space activities

This paper argues that a better understanding of strategic decision making processes in different space agencies is relevant to the successful carrying out of international cooperative undertakings in space and that this kind of information should be taken into account when decisions are to be made on engaging in a new partnership. In this context, the paper provides a general framework for the study of strategic decision making in space organisations, with particular emphasis on the influence of culture. Additionally, it offers practical examples of how differences in policy priorities, decision making environments and practices amongst space organisations can affect international cooperative endeavours. A particular concern is with the role of national and corporate cultures in creating and reflecting differences between space agencies. In order to gather information on strategic decision making processes in the space sector, a series of 30 interviews was conducted with top decision makers from NASA, ESA and the space organisations of Brazil, France, Canada, Russia, The Netherlands and the UK.     

Microbial fuel cells applied to the metabolically based detection of extraterrestrial life

Since the 1970s, when the Viking spacecrafts carried out experiments to detect microbial metabolism on the surface of Mars, the search for nonspecific methods to detect life in situ has been one of the goals of astrobiology. It is usually required that a methodology detect life independently from its composition or form and that the chosen biological signature point to a feature common to all living systems, such as the presence of metabolism. In this paper, we evaluate the use of microbial fuel cells (MFCs) for the detection of microbial life in situ. MFCs are electrochemical devices originally developed as power electrical sources and can be described as fuel cells in which the anode is submerged in a medium that contains microorganisms. These microorganisms, as part of their metabolic process, oxidize organic material, releasing electrons that contribute to the electric current, which is therefore proportional to metabolic and other redox processes. We show that power and current density values measured in MFCs that use microorganism cultures or soil samples in the anode are much larger than those obtained with a medium free of microorganisms or sterilized soil samples, respectively. In particular, we found that this is true for extremophiles, which have been proposed as potential inhabitants of extraterrestrial environments. Therefore, our results show that MFCs have the potential to be used for in situ detection of microbial life.     

Chiral symmetry breaking and complete chiral purity by thermodynamic-kinetic feedback near equilibrium: implications for the origin of biochirality

Chiral symmetry breaking occurs when a physical or chemical process spontaneously generates a large excess of one of the two enantiomers--left-handed (L) or right-handed (D)--with no preference as to which of the two enantiomers is produced. From the viewpoint of energy, these two enantiomers can exist with an equal probability, and inorganic processes that involve chiral products commonly yield a racemic mixture of both. The fact that biologically relevant molecules exist only as one of the two enantiomers is a fascinating example of complete symmetry breaking in chirality and has long intrigued the science community. The origin of this selective chirality has remained a fundamental enigma with regard to the origin of life since the time of Pasteur, some 140 years ago. Here, it is shown that two populations of chiral crystals of left and right hand cannot coexist in solution: one of the chiral populations disappears in an irreversible autocatalytic process that nurtures the other one. Final and complete chiral purity seems to be an inexorable fate in the course of the common process of growth-dissolution. This unexpected chiral symmetry breaking can be explained by the feedback between the thermodynamic control of dissolution and the kinetics of the growth process near equilibrium. This "thermodynamic-kinetic feedback near equilibrium" is established as a mechanism to achieve complete chiral purity in solid state from a previously solid racemic medium. The way in which this mechanism could operate in solutions of chiral biomolecules is described. Finally, based on this mechanism, experiments designed to search for chiral purity in a new way are proposed: chiral purity of amino acids or biopolymers is predicted in solid phase from a previously solid racemic medium. This process may have played a key role in the origin of biochirality.     

To the problem of spectrum investigation with an on-board analyser VAR

The spectrum analysis of low frequency processes on board the satellite becomes inevitable in connection with the investigation of the thin structure of specific areas in magnetosphere, main shock wave and solar wind. The necessity to perform the analysis in a rather short period of time is a peculiarity of the thin structure investigation procedure. The evaluation of drawbacks and advantages of various spectral analysis techniques shows that the discrete Fourier transform (DFT) method is most efficient for the case. It enables us to perform a parallel analysis of several processes in different frequency subranges. The amplitudes and frequencies of polyharmonic processes and spectral power of low frequency random processes are determined. The technique described in this paper has been developed at the Institute for Physics and Mechanics of the Ukr. S.S.R. Academy of Sciences to determine the amplitudes and frequencies of the polyharmonic processes components. The analyser VAR designed at the Institute is supposed to be utilized in international cosmic tests.     

The potential for lithoautotrophic life on Mars: application to shallow interfacial water environments

We developed a numerical model to assess the lithoautotrophic habitability of Mars based on metabolic energy, nutrients, water availability, and temperature. Available metabolic energy and nutrient sources were based on a laboratory-produced Mars-analog inorganic chemistry. For this specific reference chemistry, the most efficient lithoautotrophic microorganisms would use Fe(2+) as a primary metabolic electron donor and NO(3)(-) or gaseous O(2) as a terminal electron acceptor. In a closed model system, biomass production was limited by the electron donor Fe(2+) and metabolically required P, and typically amounted to approximately 800 pg of dry biomass/ml ( approximately 8,500 cells/ml). Continued growth requires propagation of microbes to new fecund environments, delivery of fresh pore fluid, or continued reaction with the host material. Within the shallow cryosphere--where oxygen can be accessed by microbes and microbes can be accessed by exploration-lithoautotrophs can function within as little as three monolayers of interfacial water formed either by adsorption from the atmosphere or in regions of ice stability where temperatures are within some tens of degrees of the ice melting point. For the selected reference host material (shergottite analog) and associated inorganic fluid chemistry, complete local reaction of the host material potentially yields a time-integrated biomass of approximately 0.1 mg of dry biomass/g of host material ( approximately 10(9) cells/g). Biomass could also be sustained where solutes can be delivered by advection (cryosuction) or diffusion in interfacial water; however, both of these processes are relatively inefficient. Lithoautotrophs in near-surface thin films of water, therefore, would optimize their metabolism by deriving energy and nutrients locally. Although the selected chemistry and associated model output indicate that lithoautotrophic microbial biomass could accrue within shallow interfacial water on Mars, it is likely that these organisms would spend long periods in maintenance or survival modes, with instantaneous biomass comparable to or less than that observed in extreme environments on Earth.     

The fluid-dynamic disturbances induced on the ISS, based on the first acceleration measurements on board the space station

The different acceleration components on the ISS that are responsible for the generation of convective motions in a fluid cell either in the presence of density gradients or in quasi-isodense processes, are analyzed. The NASA measurements of the quasi-steady and periodic acceleration on the ISS are considered and their effects on fluid-dynamic experiments are computed and discussed under different assumptions. In particular, numerical simulations are carried out to identify the relative importance of linear and pendular accelerations, due to possible rotations of the P/L around its center of mass. The effects caused by variable accelerations created by an isolation mount that exhibits an attenuation factor not constant within the payload volume, caused by the reaction forces of the umbilicals, are computed and analyzed.     

The Martian and extraterrestrial UV radiation environment. Part II: further considerations on materials and design criteria for artificial ecosystems.

Ultraviolet radiation is an important natural physical influence on organism function and ecosystem interactions. The UV radiation fluxes in extraterrestrial environments are substantially different from those experienced on Earth. On Mars, the moon and in Earth orbit they are more biologically detrimental than on Earth. Based on previously presented fluxes and biologically weighted irradiances, this paper considers in more detail measures to mitigate UV radiation damage and methods to modify extraterrestrial UV radiation environments in artificial ecosystems that use natural sunlight. The transmission characteristics of a Martian material that will mimic the terrestrial UV radiation environment are presented. Transmissivity characteristics of other Martian and lunar materials are described. Manufacturing processes for the production of plastics and glass on the lunar and Martian surface are presented with special emphasis on photobiological requirements. Novel UV absorbing configurations are suggested.     

Spatially resolved genomic, stable isotopic, and lipid analyses of a modern freshwater microbialite from cuatro ciénegas, Mexico

Abstract Microbialites are biologically mediated carbonate deposits found in diverse environments worldwide. To explore the organisms and processes involved in microbialite formation, this study integrated genomic, lipid, and both organic and inorganic stable isotopic analyses to examine five discrete depth horizons spanning the surface 25?mm of a modern freshwater microbialite from Cuatro Ciénegas, Mexico. Distinct bacterial communities and geochemical signatures were observed in each microbialite layer. Photoautotrophic organisms accounted for approximately 65% of the sequences in the surface community and produced biomass with distinctive lipid biomarker and isotopic (δ(13)C) signatures. This photoautotrophic biomass was efficiently degraded in the deeper layers by heterotrophic organisms, primarily sulfate-reducing proteobacteria. Two spatially distinct zones of carbonate precipitation were observed within the microbialite, with the first zone corresponding to the phototroph-dominated portion of the microbialite and the second zone associated with the presence of sulfate-reducing heterotrophs. The coupling of photoautotrophic production, heterotrophic decomposition, and remineralization of organic matter led to the incorporation of a characteristic biogenic signature into the inorganic CaCO(3) matrix. Overall, spatially resolved multidisciplinary analyses of the microbialite enabled correlations to be made between the distribution of specific organisms, precipitation of carbonate, and preservation of unique lipid and isotopic geochemical signatures. These findings are critical for understanding the formation of modern microbialites and have implications for the interpretation of ancient microbialite records. Key Words: Microbial ecology-Microbe-mineral interactions-Microbial mats-Stromatolites-Genomics. Astrobiology 12, 685-698.     

Structural and functional changes in man accompanying the weightlessness in "Skylab" flights: a mathematical approach

The present paper reports a kinetic analysis of changes of some physiological parameters, obtained from international literature, after changes in gravitational environment. The overall phenomenology of the adaptation to weightlessness is characterized by a rapid process followed by a slow one. The two processes show half time values differing by about five times. Also in the case of readaptation to gravity, after recovery on the Earth, two well resolved processes, showing different half time values, are observed. It is of interest to notice that the rate of response to weightlessness is lower than that to gravity. Of course, the half time values observed depend on the different physiological parameters considered. In any case, the experimental data suggest a general trend of many adaptive changes, that may all be described by a simple mathematical model.     

Main results and experience obtained on Mir space station and experiment program for Russian segment of ISS

This article presents main scientific and practical results obtained in course of scientific and applied research and experiments on Mir space station. Based on Mir experience, processes of research program formation for the Russian Segment of the ISS are briefly described. The major trends of activities planned in the frames of these programs as well as preliminary results of increment research programs implementation in the ISS' first missions are also presented.     

Evaluation of safety of hypobaric decompressions and EVA from positions of probabilistic theory

Formation and subsequent evolution of gas bubbles in blood and tissues of subjects exposed to decompression are casual processes in their nature. Such character of bubbling processes in a body predetermines probabilistic character of decompression sickness (DCS) incidence in divers, aviators and astronauts. Our original probabilistic theory of decompression safety is based on stochastic models of these processes and on the concept of critical volume of a free gas phase in body tissues. From positions of this theory, the probability of DCS incidence during single-stage decompressions and during hypobaric decompressions under EVA in particular, is defined by the distribution of possible values of nucleation efficiency in "pain" tissues and by its critical significance depended on the parameters of a concrete decompression. In the present study the following is shown: 1) the dimensionless index of critical nucleation efficiency for "pain" body tissues is a more adequate index of decompression stress in comparison with Tissue Ratio, TR; 2) a priory the decompression under EVA performed according to the Russian protocol is more safe than decompression under EVA performed in accordance with the U.S. protocol; 3) the Russian space suit operated at a higher pressure and having a higher "rigidity" induces a stronger inhibition of mechanisms of cavitation and gas bubbles formation in tissues of a subject located in it, and by that provides a more considerable reduction of the DCS risk during real EVA performance.     

非线性连接结构对一个典型卫星频率漂移的影响

文章针对一个典型的卫星桁架结构建立了三自由度运动模型,分别引入间隙非线性和Iwan模型描述了非线性连接结构;利用数值方法研究了系统的频率漂移,讨论了间隙大小、预紧力（重力）、系统阻尼、随机间隙及Iwan模型的参数对频率漂移的影响;对比了两种非线性连接结构下系统频率漂移的特点。研究表明,间隙结合预紧力条件可令结构呈现“硬弹簧-软弹簧”特性的频率漂移,迟滞和间隙两种非线性引起的频漂在幅频曲线上表现出不同的特点。     

3σ准则应用对结构疲劳损伤评价的影响分析

工程研究中经常要应用3σ准则来实现随机振动过程的时域、频域转换。文章应用疲劳损伤分析的频域方法,讨论了3σ准则的应用对于振动疲劳损伤累积评价结果的影响。应用满足Rayleigh分布的窄带模型及其4种典型的修正方法分析疲劳影响因子,数值模拟结果表明,3σ截断后的疲劳损伤累积仅占全部疲劳损伤累积的约70%（取m=6）,与材料疲劳参数m有关,且基于窄带模型的疲劳影响因子分析方法具有一定程度的通用性。     

核热推进载人火星探测方案设计

针对载人火星探测任务，结合我国现有技术基础，提出我国载人火星探测方案，重点研究载人火星探测任务推进系统的设计。首先，综合考虑载人深空探测任务的约束，采用Pork-Chop图设计了适用于不同任务场景的转移轨迹；然后，参考我国空间站技术，基于核热推进系统设计了我国载人火星探测任务的飞船；最后，对核热推进系统的发动机台数和推力进行了优化，得到了适用于不同任务场景的最优推进系统组合方案。本文所研究内容为我国未来载人火星探测任务提供了有益参考。     

The Impact of Variable Accelerations on Crystal Growth onboard Spacecraft by the Floating Zone Method

The numerical investigation of the impact of time-dependent accelerations (vibrations) on the flow and heat and mass transfer in the melt is carried out for the case of modeling the crystal growth by the floating zone method under conditions of microgravity that exist onboard spacecraft. The effects of the Archimedean buoyancy force and of vibrations of the free surface of fluid are considered separately. When solving the problem of the effect of the vibrations of the free surface of fluid, the previously obtained data were used. It is shown that vibrations of the free surface have a much stronger effect on the processes under consideration than the buoyancy. Some problems that are related to the newly discovered effects are discussed. The use of vibroprotected systems and a rotating magnetic field can help solve these problems. We plan to continue our investigations in future spacecraft experiments, in particular, at the International Space Station, which is under construction at the moment.     

用于声纳设备中的分布式遥控遥测技术

给出了一种在声纳站中用ＰＣ型微处理机和ＭＣＳ－８０３１型单片机构成的分布式数据采集和遥控系统。该系统利用ＰＣ机具有良好的人机界面、丰富的软件资源及灵敏多样的显示功能，以及８０３１系列单片机的多机通讯功能相结合。对相距几十米甚至几百米距离上的下位机进行控制和数据采集，在ＰＣ机的ＣＲＴ上进行显示和处理。     

Regeneration of water at space stations

The history, current status and future prospects of water recovery at space stations are discussed. Due to energy, space and mass limitations physical/chemical processes have been used and will be used in water recovery systems of space stations in the near future. Based on the experience in operation of Russian space stations Salut, Mir and International space station (ISS) the systems for water recovery from humidity condensate and urine are described. A perspective physical/chemical system for water supply will be composed of an integrated system for water recovery from humidity condensate, green house condensate, water from carbon dioxide reduction system and condensate from urine system; a system for water reclamation from urine; hygiene water processing system and a water storage system. Innovative processes and new water recovery systems intended for Lunar and Mars missions have to be tested on the international space station.     

Consequences of collisions of natural cosmic bodies with the Earth’s atmosphere and surface

Possible consequences of collisions of natural cosmic bodies with the Earth’s atmosphere and surface are described. The methodological basis of classification of consequences is the solution of meteor physics equations characterizing the trajectory of a body in the atmosphere, namely, the dependence of the body’s velocity and mass on the flight altitude. The solution depends on two dimensionless parameters characterizing the drag altitude and the role of mass loss by a meteoroid during its motion in the atmosphere. Depending on values of these parameters, the degree of effect on the planetary surface considerably changes. In particular, the conditions of cratering and meteorite fall on the planetary surface are obtained. The results are presented in a simple analytical form. They quite match to the real events considered in the paper. Recommendations are given on further investigations into the important problem of interaction of cosmic bodies with planetary atmospheres.     

Numerical Simulation of Geomagnetic Trapping of Anomalous Cosmic Ray Ions

A method for simulating the propagation processes for ions of the anomalous component of cosmic rays in the Earth's magnetic field is described with allowance made for a step-by-step stripping of the ions in the residual atmosphere and their trapping by the geomagnetic field. Numerical results are presented for the geomagnetic trapping of high-energy singly charged oxygen ions penetrating into the stripping region from interplanetary space.