Resistance of bacterial endospores to outer space for planetary protection purposes--experiment PROTECT of the EXPOSE-E mission

Spore-forming bacteria are of particular concern in the context of planetary protection because their tough endospores may withstand certain sterilization procedures as well as the harsh environments of outer space or planetary surfaces. To test their hardiness on a hypothetical mission to Mars, spores of Bacillus subtilis 168 and Bacillus pumilus SAFR-032 were exposed for 1.5 years to selected parameters of space in the experiment PROTECT during the EXPOSE-E mission on board the International Space Station. Mounted as dry layers on spacecraft-qualified aluminum coupons, the "trip to Mars" spores experienced space vacuum, cosmic and extraterrestrial solar radiation, and temperature fluctuations, whereas the "stay on Mars" spores were subjected to a simulated martian environment that included atmospheric pressure and composition, and UV and cosmic radiation. The survival of spores from both assays was determined after retrieval. It was clearly shown that solar extraterrestrial UV radiation (λ≥110?nm) as well as the martian UV spectrum (λ≥200?nm) was the most deleterious factor applied; in some samples only a few survivors were recovered from spores exposed in monolayers. Spores in multilayers survived better by several orders of magnitude. All other environmental parameters encountered by the "trip to Mars" or "stay on Mars" spores did little harm to the spores, which showed about 50% survival or more. The data demonstrate the high chance of survival of spores on a Mars mission, if protected against solar irradiation. These results will have implications for planetary protection considerations.     

Survival of Bacillus pumilus spores for a prolonged period of time in real space conditions

To prevent forward contamination and maintain the scientific integrity of future life-detection missions, it is important to characterize and attempt to eliminate terrestrial microorganisms associated with exploratory spacecraft and landing vehicles. Among the organisms isolated from spacecraft-associated surfaces, spores of Bacillus pumilus SAFR-032 exhibited unusually high resistance to decontamination techniques such as UV radiation and peroxide treatment. Subsequently, B. pumilus SAFR-032 was flown to the International Space Station (ISS) and exposed to a variety of space conditions via the European Technology Exposure Facility (EuTEF). After 18 months of exposure in the EXPOSE facility of the European Space Agency (ESA) on EuTEF under dark space conditions, SAFR-032 spores showed 10-40% survivability, whereas a survival rate of 85-100% was observed when these spores were kept aboard the ISS under dark simulated martian atmospheric conditions. In contrast, when UV (>110?nm) was applied on SAFR-032 spores for the same time period and under the same conditions used in EXPOSE, a ～7-log reduction in viability was observed. A parallel experiment was conducted on Earth with identical samples under simulated space conditions. Spores exposed to ground simulations showed less of a reduction in viability when compared with the "real space" exposed spores (～3-log reduction in viability for "UV-Mars," and ～4-log reduction in viability for "UV-Space"). A comparative proteomics analysis indicated that proteins conferring resistant traits (superoxide dismutase) were present in higher concentration in space-exposed spores when compared to controls. Also, the first-generation cells and spores derived from space-exposed samples exhibited elevated UVC resistance when compared with their ground control counterparts. The data generated are important for calculating the probability and mechanisms of microbial survival in space conditions and assessing microbial contaminants as risks for forward contamination and in situ life detection.     

Suborbital and low-thermospheric experiments using sounding rockets in Taiwan

The suborbital flight is a kind of flight, which reaches the space and then comes back to ground without completing one orbital revolution. The atmospheric thermosphere extends from 85 km to 600 km in altitude. Therefore, the suborbital and low-thermospheric experiments to be performed at altitude below 300 km can be combined using the sounding rocket. These experiments include rocket staging, fairing separation, ultrasonic flight, reentry, aerobrake and recovery test, ultraviolet and ionization observations, ozone measurement, etc. The advent of Taiwan's sub-orbital and thermospheric experiments project can be traced back to 1997. This is the year Taiwan's National Space Organization (NSPO) was assigned to be responsible for procuring the sounding rocket for applications in science experiments and space technology research effort. From 1997 to 2010, 8 launches have been completed including one experimental hybrid rocket. All onboard instruments and sensors for sub-orbital and low-thermospheric experiments are developed and integrated by the domestic universities. More launches have been planned in the future. Opportunities for international cooperation in developing new instruments and payloads for future experiments will be possible.     

Sounding rocket experiment of bare electrodynamic tether system

An overview of a sounding rocket, S-520-25th, project on space tether technology experiment is presented. The project is prepared by an international research group consisting of Japanese, European, American, and Australian researchers. The sounding rocket will be assembled by the ISAS/JAXA and will be launched in the summer of 2009. The sounding rocket mission includes two engineering experiments and two scientific experiments. These experiments consist of the deployment of bare electrodynamic tape tether in space, a quick ignition test of hollow cathode system in space, the demonstration of bare electrodynamic tether system in space, and the test of the OML (orbital-motion-limit) current collection theory.     

Survival of spores of the UV-resistant Bacillus subtilis strain MW01 after exposure to low-earth orbit and simulated martian conditions: data from the space experiment ADAPT on EXPOSE-E

In the space experiment "Molecular adaptation strategies of microorganisms to different space and planetary UV climate conditions" (ADAPT), bacterial endospores of the highly UV-resistant Bacillus subtilis strain MW01 were exposed to low-Earth orbit (LEO) and simulated martian surface conditions for 559 days on board the European Space Agency's exposure facility EXPOSE-E, mounted outside the International Space Station. The survival of B. subtilis MW01 spores from both assays (LEO and simulated martian conditions) was determined by a colony-formation assay after retrieval. It was clearly shown that solar extraterrestrial UV radiation (λ≥110?nm) as well as the martian UV spectrum (λ≥200?nm) was the most deleterious factor applied; in some samples only a few spore survivors were recovered from B. subtilis MW01 spores exposed in monolayers. However, if shielded from solar irradiation, about 8% of MW01 spores survived in LEO conditions, and 100% survived in simulated martian conditions, compared to the laboratory controls. The results demonstrate the effect of shielding against the high inactivation potential of extraterrestrial solar UV radiation, which limits the chances of survival of even the highly UV-resistant strain of B. subtilis MW01 in the harsh environments of outer space and the martian surface.     

Microbial rock inhabitants survive hypervelocity impacts on Mars-like host planets: first phase of lithopanspermia experimentally tested

The scenario of lithopanspermia describes the viable transport of microorganisms via meteorites. To test the first step of lithopanspermia, i.e., the impact ejection from a planet, systematic shock recovery experiments within a pressure range observed in martian meteorites (5-50 GPa) were performed with dry layers of microorganisms (spores of Bacillus subtilis, cells of the endolithic cyanobacterium Chroococcidiopsis, and thalli and ascocarps of the lichen Xanthoria elegans) sandwiched between gabbro discs (martian analogue rock). Actual shock pressures were determined by refractive index measurements and Raman spectroscopy, and shock temperature profiles were calculated. Pressure-effect curves were constructed for survival of B. subtilis spores and Chroococcidiopsis cells from the number of colony-forming units, and for vitality of the photobiont and mycobiont of Xanthoria elegans from confocal laser scanning microscopy after live/dead staining (FUN-I). A vital launch window for the transport of rock-colonizing microorganisms from a Mars-like planet was inferred, which encompasses shock pressures in the range of 5 to about 40 GPa for the bacterial endospores and the lichens, and a more limited shock pressure range for the cyanobacterium (from 5-10 GPa). The results support concepts of viable impact ejections from Mars-like planets and the possibility of reseeding early Earth after asteroid cataclysms.     

The O/OREOS mission: first science data from the Space Environment Survivability of Living Organisms (SESLO) payload

We report the first telemetered spaceflight science results from the orbiting Space Environment Survivability of Living Organisms (SESLO) experiment, executed by one of the two 10?cm cube-format payloads aboard the 5.5?kg Organism/Organic Exposure to Orbital Stresses (O/OREOS) free-flying nanosatellite. The O/OREOS spacecraft was launched successfully to a 72° inclination, 650?km Earth orbit on 19 November 2010. This satellite provides access to the radiation environment of space in relatively weak regions of Earth's protective magnetosphere as it passes close to the north and south magnetic poles; the total dose rate is about 15 times that in the orbit of the International Space Station. The SESLO experiment measures the long-term survival, germination, and growth responses, including metabolic activity, of Bacillus subtilis spores exposed to the microgravity, ionizing radiation, and heavy-ion bombardment of its high-inclination orbit. Six microwells containing wild-type (168) and six more containing radiation-sensitive mutant (WN1087) strains of dried B. subtilis spores were rehydrated with nutrient medium after 14 days in space to allow the spores to germinate and grow. Similarly, the same distribution of organisms in a different set of microwells was rehydrated with nutrient medium after 97 days in space. The nutrient medium included the redox dye Alamar blue, which changes color in response to cellular metabolic activity. Three-color transmitted intensity measurements of all microwells were telemetered to Earth within days of each of the 48?h growth experiments. We report here on the evaluation and interpretation of these spaceflight data in comparison to delayed-synchronous laboratory ground control experiments.     

Floating zone experiments with germanium crystals in sounding rockets

A floating zone experiment with a coated surface was performed in a sounding rocket. Analysis of the segregation showed that elimination of free surfaces decreased the convection in the liquid zone. Analysis of radial segregation during floating zone crystal growth under different experimental conditions demonstrated high sensitivity to interface shape and to convection. Under conditions of very weak convection, microgravity and coated surfaces, dopant concentration fields giving severe radial segregation were built up.     

空间目标高分辨距离像运动参数估计

建立了宽带线性调频雷达信号的空间目标回波模型，对目标回波信号进行了分析，分析了空间目标高速运动对目标高分辨一维距离像的影响，提出了用多项式变换进行参数估计的方法。利用估计参数进行运动补偿，消除目标速度和加速度对目标一维距离像的影响。最后给出了仿真结果，验证了本文的分析结果正确和算法有效。     

中国航天固体火箭技术的发展

叙述了20世纪50年代以来中国航天固体火箭推进技术的发展历程，介绍了9种最具代表性的固体火箭发动机的技术特征、研制过程、地面试验和飞行情况，这些发动机分别应用于中国的探空火箭、运载火箭上面级和应用卫星变轨系统。文中还简要地评述了中国固体推进各单项技术的发展水平。     

固液混合火箭发动机研究进展

固液火箭发动机因其推进剂能量较高、安全性好、易实现推力调节、可实现多次启停、药柱稳定型好、温度敏感性低、环保性佳和经济性好的特点,十分符合下一代航天平台绿色环保、智能随控、快速响应的发展需求,在探空火箭、亚轨道飞行器、靶标、小型运载火箭、助推器、上面级动力系统、姿轨控动力系统、着陆器和其他许多民用商业航天领域中都具有良好的应用前景。分析了固液火箭发动机的国内外发展现状及发展趋势,对国内外固液火箭发动机相关的典型项目、工程应用和关键技术发展情况进行了回顾和总结,并以此为基础总结固液火箭发动机技术的发展趋势和有待进一步突破的关键技术。     

UV photochemistry of DNA in vitro and in Bacillus subtilis spores at earth-ambient and low atmospheric pressure: implications for spore survival on other planets or moons in the solar system

Two major parameters influencing the survival of Bacillus subtilis spores in space and on bodies within the Solar System are UV radiation and vacuum, both of which induce inactivating damage to DNA. To date, however, spore survival and DNA photochemistry have been explored only at the extremes of Earth-normal atmospheric pressure (101.3 kPa) and at simulated space vacuum (10(-3)-10(-6) Pa). In this study, wild-type spores, mutant spores lacking alpha/beta-type small, acid-soluble spore proteins (SASP), naked DNA, and complexes between SASP SspC and DNA were exposed simultaneously to UV (254 nm) at intermediate pressure (1-2 Pa), and the UV photoproducts cis,syn-thymine-thymine cyclobutane dimer (c,sTT), trans,syn-thymine-thymine cyclobutane dimer (t,sTT), and "spore photoproduct" (SP) were quantified. At 101.3 kPa, UV-treated wild-type spores accumulated only SP, but spores treated with UV radiation at 1-2 Pa exhibited a spectrum of DNA damage similar to that of spores treated at 10(-6) Pa, with accumulation of SP, c,sTT, and t,sTT. The presence or absence of alpha/beta-type SASP in spores was partly responsible for the shift observed between levels of SP and c,sTT, but not t,sTT. The changes observed in spore DNA photochemistry at 1-2 Pa in vivo were not reproduced by irradiation of naked DNA or SspC:DNA complexes in vitro, suggesting that factors other than SASP are involved in spore DNA photochemistry at low pressure.     

激光驱动飞片系统模拟空间碎片超高速撞击

文章介绍了北京卫星环境工程研究所采用激光驱动飞片系统将厚度5μm的铝质飞片发射到8.3 km/s.该驱动系统采用调Q钕玻璃激光,脉宽15 ns,最大能量达到20 J.针对热控材料、舷窗玻璃及OSR等外部表面功能材料开展了超高速撞击实验研究,取得初步研究结果.实验结果表明激光驱动飞片技术可很好地用于模拟微流星/空间碎片超高速撞击效应研究.     

Sounding rocket developments in Spain

This paper contemplates the efforts and developments in the field of sounding rockets carried out in Spain from the decade of the 1960s to the early 1990s when the use of such vehicles was abandoned worldwide.The initial sounding rocket planning within the National Space Research Programs around 1964 (when Spain joined ESRO) is presented.The status of the rocket technology in Spain in 1964 is analysed, reviewing the main technology gaps and the way they were filled to make the planned developments possible.Three Spanish sounding rockets are presented: the INTA-255 (150 km apogee with formative objectives, first launched in 1969), the INTA-300 (300 km apogee with high characteristics and commercial capabilities, first launched in 1974) and the INTA-100 (115 km apogee being finally a totally national product, first launched in 1980).Some guided rocket vehicle projects which were based, on some way, on the previous sounding rockets activities are also mentioned in this paper.     

Bacillus subtilis spore survival and expression of germination-induced bioluminescence after prolonged incubation under simulated Mars atmospheric pressure and composition: implications for planetary protection and lithopanspermia

Bacterial endospores in the genus Bacillus are considered good models for studying interplanetary transfer of microbes by natural or human processes. Although spore survival during transfer itself has been the subject of considerable study, the fate of spores in extraterrestrial environments has received less attention. In this report we subjected spores of a strain of Bacillus subtilis, containing luciferase resulting from expression of an sspB-luxAB gene fusion, to simulated martian atmospheric pressure (7-18 mbar) and composition (100% CO(2)) for up to 19 days in a Mars simulation chamber. We report here that survival was similar between spores exposed to Earth conditions and spores exposed up to 19 days to simulated martian conditions. However, germination-induced bioluminescence was lower in spores exposed to simulated martian atmosphere, which suggests sublethal impairment of some endogenous spore germination processes.     

粉尘静电加速设备原理及发展现状

微米级空间碎片数量巨大,与航天器的碰撞概率很高,其累积效应会影响航天器的性能.国外已发展了粉尘静电加速设备用于模拟微米级空间碎片的撞击效应.文章对粉尘静电模拟加速设备的原理和发展现状进行了介绍,并对该种设备的优缺点进行了分析讨论.     

Optimal trajectories for the aeroassisted flight experiment

This paper deals with the determination of optimal trajectories for the aeroassisted flight experiment (AFE). The intent of this experiment is to simulate a GEO-to-LEO transfer, where GEO denotes a geosynchronous Earth orbit and LEO denotes a low Earth orbit. Specifically, the AFE spacecraft is released from the Space Shuttle and is accelerated by means of a solid rocket motor toward Earth, so as to achieve atmospheric entry conditions identical with those of a spacecraft returning from GEO. During the atmospheric pass, the angle of attack is kept constant, and the angle of bank is controlled in such a way that the following conditions are satisfied: (a) the atmospheric velocity depletion is such that, after exiting, the AFE spacecraft first ascends to a specified apogee and then descends to a specified perigee; and (b) the exit orbital plane is identical with the entry orbital plane. The final maneuver, not analyzed here, includes the rendezvous with and the capture by the Space Shuttle. In this paper, the trajectories of an AFE spacecraft are analyzed in a 3D space, employing the full system of 6 ODEs describing the atmospheric pass. The atmospheric entry conditions are given, and the atmospheric exit conditions are adjusted in such a way that requirements (a) and (b) are met, while simultaneously minimizing the total characteristic velocity, hence the propellant consumption required for orbital transfer. Two possible transfers are considered: indirect ascent (IA) to a 178 NM perigee via a 197 NM apogee; and direct ascent (DA) to a 178 NM apogee. For both transfers, two cases are investigated: (i) the bank angle is continuously variable; and (ii) the trajectory is divided into segments along which the bank angle is constant. For case (ii), the following subcases are studied; 2, 3, 4 and 5 segments; because the time duration of each segment is optimized, the above subcases involve 4, 6, 8 and 10 parameters, respectively. It is shown that the optimal trajectories of cases (i) and (ii) coalesce into a single trajectory: a two-subarc trajectory, with the bank angle constant in each subarc (bang-bang control). Specifically, the bank angle is near 180° in the atmospheric entry phase (positive lift projection phase) and is near 0° in the atmospheric exit phase (negative lift projection phase). It is also shown that, during the atmospheric pass, the peak values of the changes of the orbital inclination and the longitude of the ascending node are nearly zero; hence, the peak value of the wedge angle (angle between the instantaneous orbital plane and the initial orbital plane) is nearly zero. This means that the motion of the spacecraft is nearly planar in an inertial space.     

多GNSS掩星大气探测卫星星座设计

为减少无线电掩星(RO)大气探测星座的卫星数量并增加探测数据量,将北斗（BD）和GPS、Galileo、GLONASS共同作为探测信源,提出一种多全球导航卫星系统（GNSS）掩星大气探测星座概念和优化设计方法。融合先验大气模型和二维射线追踪算法,建立兼容多GNSS信源的掩星事件前向模拟算法,实现掩星事件快速精确仿真;给出多GNSS掩星大气探测星座参数对探测性能的影响特性,降低了星座模型的复杂度;并利用改进的蚁群算法实现星座参数寻优。设计结果与COSMICII星座相比,卫星数量减少2颗,探测数据量增加了40%,探测均匀性提高了67%。     

Planetary quarantine in the solar system. Survival rates of some terrestrial organisms under simulated space conditions by proton irradiation

We have been studying the survival rates of some species of terrestrial unicellular and multicellular organism (viruses, bacteria, yeasts, fungi, algae, etc.) under simulated interstellar conditions, in connection with planetary quarantine. The interstellar environment in the solar system has been simulated by low temperature, high vacuum (77 K, 4 x 10(-8) torr), and proton irradiation from a Van de Graaff generator. After exposure to a barrage of protons corresponding to about 250 years of irradiation in solar space, tobacco mosaic virus, Bacillus subtilis spores, Staphylococcus aureus, Micrococcus flavus, Aspergillus niger spores, and Clostridium mangenoti spores showed survival rates of 82, 45, 74, 13, 28, and 25%, respectively.     

Potential effects of cosmic dust and rocket exhaust particles on spacecraft charging

Attention is called to the importance of including the roles of cosmic dust particles and rocket exhaust particles in the detailed analysis of spacecraft charging effects, arcing and power drains due to leakage currents. Aspects of the problem pertaining to both low and high (geosynchronous) Earth orbit are discussed. Recommendations are made to assessing the long-term effects of hypervelocity impacts of these particles.