超声波预处理的人血小板冻干保存实验

在热孵化的基础上增加超声波辐射以强化海藻糖载入血小板,将处理后的血小板进行冷冻干燥保存,以未经超声波辐射仅受热孵化预处理的血小板为对照组,研究超声波预处理对人血小板冻干保存的影响。结果表明,经25 kHz,0.8 W/cm2的超声波辐射30 min的血小板细胞内海藻糖浓度达到(28.9±4.48)mmol/L,比对照组提高118.9%,超声处理后样品经血液学检测各项指标均为正常。两组血小板同时进行冻干后复水,经检验,样品组冻干血小板数值恢复率为(83.3±4.88)%,血小板分布宽度值(Platelet distribution width,PDW)为(18.9±1.55)%;对照组血小板数值恢复率为(80.8±4.54)%,PDW值为(18.1±1.15)%,两者不存在统计学差异。据此实验结果可以认为,超声波载糖法用于血小板冻干预处理是可行的,这为进一步进行血小板冻干研究提供了参考。

Experimental Study on Lyophilization Preservation of Human Platelets Pretreated by Ultrasound

Trehalose can be loaded into platelets cells by heat incubation,and ultrasound is employed to enhance the loading of trehalose into human platelets.Then treated platelets are freeze-dried and rehydrated.The influence of the pre-treatment of ultrasound radiation on lyophilized platelets are studied,and the platelets only subjected to heat incubation are used as the controls.The results show that the intracellular trahalose concentration is(28.9±4.48) mmol/L after radiation for 30 min by ultrasound(frequency of 25 kHz,intensity of 0.8 W/cm2).This concentration value is 118.9% higher than that of the controls.And the treated platelets show normal characteristics by several hematology examination.After freeze-drying and rehydration,the recovery of the freeze-dried samples is(83.3±4.88)%,PDW is(18.9±1.55)%,while for the control ones,the recovery and the PDW are(80.8±4.54)% and(18.1±1.15)%,respectively.There is no significant differences.Based on the experimental results,the ultrasound used as a pre-treatment of platelets lyophilization is feasible.