Optimal conditions for simulating microgravity employing NASA designed rotating wall vessels |
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| Institution: | 1. Institute of Crystallography, National Research Council of Italy (CNR), Via Salaria km 29.300, 00015 Monterotondo Scalo, Rome, Italy;2. Department of Molecular Medicine, Center for Health Technologies (CHT), University of Pavia, Via Taramelli 3/b, 27100 Pavia, Italy;3. Department of Pharmacological and Biomolecular Sciences, University of Milan, Via D. Trentacoste 2, 20134 Milan, Italy;4. Department of Public Health, Experimental Medicine and Forensics, University of Pavia, V.le Forlanini 8, Pavia, Italy;5. Department of Occupational Medicine, Toxicology and Environmental Risks, S. Maugeri Foundation, IRCCS, Via S. Boezio 28, 27100 Pavia, Italy |
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| Abstract: | This article discusses the requirements to successfully simulate certain aspects of microgravity employing the NASA designed HARV in the laboratory. Presence of low fluid shear, a clear understanding of the role of the spin of the microcarrier/cell about its own axis, availability of adequate mass transport of nutrients and oxygen, the elimination of collisions between microcarriers and between the microcarrier and walls of the bioreactor, and the use of adequate and appropriate controls are some of the important aspects that enable a successful simulation of certain aspects of microgravity. Results of numerical simulation of the above aspects have been presented and discussed. |
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