W-reactivation and W-mutagenesis in phage phi X 174. |
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| Authors: | F Yatagai T Takahashi S Kitayama |
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| Institution: | The Institute of Physical and Chemical Research, Wako-shi, Japan. |
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| Abstract: | W-reactivation (WR) and W-mutagenesis (WM) were observed after irradiation of phage chi X 174 with He and N ions (4.6 MeV/amu). The relative amount of phage RFI DNA (RARFI) extracted from the infected cells was determined by agarose gel electrophoresis. It was found that RARFI was increased by prior UV-irradiation of host cells and this increase corresponded to the efficiency of WR. |
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