太空环境对肿瘤细胞生理特性的影响

将3种肿瘤细胞搭载于“神舟4号”的卫星返回舱内,经过7天太空飞行,回收后对存活细胞进行单克隆化,观察细胞形态,并测定了细胞周期、黏附力及细胞因子表达.结果显示,经太空飞行,小鼠黑色素瘤B16细胞的周期发生改变,G1期细胞明显增多(p<0.05),并表现多种细胞形态;人肺鳞癌细胞L78对血管内皮细胞黏附力明显减弱,但经传代培养其黏附力恢复且超过对照组细胞;Caski细胞IL-2、IL-8、TNF和TGF的表达均明显增加,而L78细胞上述4种细胞因子的表达均显著下降.结论,太空环境可影响肿瘤细胞的某些生理特性,但可否影响肿瘤细胞的免疫原性,仍需做进一步的实验.

EFFECTS ON PHYSIOLOGICAL CHARACTERISTICS OF TUMOR CELL LINES BY SPACE ENVIRONMENT

To investigate the effects on physiological characteristics of tumor cell lines by space environment and inaugurate a new pathway of immune therapy in cancer, three tumor cell lines were carried in the returning module of spacecraft launched by Shenzhou-4 (SZ-4). Three cell lines were returned after 7 days' flying in space. All survival cells were monocoloned and the cell cycle, morphology, adhesive force and expression of cytokines were studied. The cell cycle of mouse melanoma B16 cells was changed after the flight. Comparing with the control group on the ground, the cell number of G1 phase was increased markedly (p < 0.05). With that simulta-neously, B16 cells showed multiple cell morphological changes. Adhesion to vascular endothelial cell was decreased in human lung carcinoma L78 cells, however, it was enhanced after passage for 2-3 times in L78 cells. The expression of cytokine IL-2, IL-8, TNF and TGF were improved in human cervical carcinoma Caski cells. In contrast, the expression of these cytokines was reduced in L78 cells. All these results revealed that physiological function of tumor cells were affected by the space environment. Whether the immunity was strengthened in tumor cell should be further studied.